• Title/Summary/Keyword: Cultivated Technology

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Current technology and industrialization status of cell-cultivated meat

  • Seung Yun Lee;Da Young Lee;Seung Hyeon Yun;Juhyun Lee;Ermie Jr Mariano;Jinmo Park;Yeongwoo Choi;Dahee Han;Jin Soo Kim;Sun Jin Hur
    • Journal of Animal Science and Technology
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    • v.66 no.1
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    • pp.1-30
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    • 2024
  • Interest and investment in cultivated meat are increasing because of the realization that it can effectively supply sufficient food resources and reduce the use of livestock. Nevertheless, accurate information on the specific technologies used for cultivated meat production and the characteristics of cultivated meat is lacking. Authorization for the use of cultivated meat is already underway in the United States, Singapore, and Israel, and other major countries are also expected to approve cultivated meat as food once the details of the intricate process of producing cultivated meat, which encompasses stages such as cell proliferation, differentiation, maturation, and assembly, is thoroughly established. The development and standardization of mass production processes and safety evaluations must precede the industrialization and use of cultivated meat as food. However, the technology for the industrialization of cultivated meat is still in its nascent stage, and the mass production process has not yet been established. The mass production process of cultivated meat may not be easy to disclose because it is related to the interests of several companies or research teams. However, the overall research flow shows that equipment development for mass production and cell acquisition, proliferation, and differentiation, as well as for three-dimensional production supports and bioreactors have not yet been completed. Therefore, additional research on the mass production process and safety of cultivated meat is essential. The consumer's trust in the cultivated meat products and production technologies recently disclosed by some companies should also be analyzed and considered for guiding future developments in this industry. Furthermore, close monitoring by academia and the government will be necessary to identify fraud in the cultivated meat industry.

Proteomic analysis of amino acid metabolism differences between wild and cultivated Panax ginseng

  • Sun, Hang;Liu, Fangbing;Sun, Liwei;Liu, Jianzeng;Wang, Manying;Chen, Xuenan;Xu, Xiaohao;Ma, Rui;Feng, Kai;Jiang, Rui
    • Journal of Ginseng Research
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    • v.40 no.2
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    • pp.113-120
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    • 2016
  • Background: The present study aimed to compare the relative abundance of proteins and amino acid metabolites to explore the mechanisms underlying the difference between wild and cultivated ginseng (Panax ginseng Meyer) at the amino acid level. Methods: Two-dimensional polyacrylamide gel electrophoresis and isobaric tags for relative and absolute quantitation were used to identify the differential abundance of proteins between wild and cultivated ginseng. Total amino acids in wild and cultivated ginseng were compared using an automated amino acid analyzer. The activities of amino acid metabolism-related enzymes and the contents of intermediate metabolites between wild and cultivated ginseng were measured using enzyme-linked immunosorbent assay and spectrophotometric methods. Results: Our results showed that the contents of 14 types of amino acids were higher in wild ginseng compared with cultivated ginseng. The amino acid metabolism-related enzymes and their derivatives, such as glutamate decarboxylase and S-adenosylmethionine, all had high levels of accumulation in wild ginseng. The accumulation of sulfur amino acid synthesis-related proteins, such as methionine synthase, was also higher in wild ginseng. In addition, glycolysis and tricarboxylic acid cycle-related enzymes as well as their intermediates had high levels of accumulation in wild ginseng. Conclusion: This study elucidates the differences in amino acids between wild and cultivated ginseng. These results will provide a reference for further studies on the medicinal functions of wild ginseng.

A study on the hydroponic cultivation of Chinese cabbage for kimchi (김치용 배추의 수경재배에 관한 연구)

  • 한덕철;문성원;김혜자;조재선
    • Korean journal of food and cookery science
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    • v.17 no.5
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    • pp.510-516
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    • 2001
  • Hydroponic cultivation is a technology of raising crops without use of soil. Generally farmers use the method of DFT(deep flow technology)to grow leafy or fruity vegetables; however, systematic and scientific researches are insufficient on this matter. This study investigated the possibility of cultivating Chinese cabbage steadily year long by using the method of DFT. Chinese cabbage was cultivated hydroponically with and without Ge addition, used to prepare kimchi, and the chemical and microbiological characteristics of kimchi were compared. The basic hydroponic cultivation condition was as follows: 30 days after seeding, the raised seeds were moved to a hydroponic bed and given underground water for 3 days so the roots grow normally Standard nutrient solution was provided and the early electric conductivity concentration was maintained between 1.5∼2.5 thickness. The temperature of the solution was maintained between 10 ∼25$^{\circ}C$ to allow the growth of Chinese cabbage. When soil-cultivated, organically cultivated and hydroponically cultivated Chinese cabbages were compared, hydroponically cultivated cabbages were smaller in size and showed less ability to build up and fold leaves into a head, but showed better quality than organically cultivated cabbages. The contents of protein and fat showed no significant differences. The contents of water. Ca, P, Fe, Vitamin A and Niacin were higher in control and Ge-added cabbages compared with soil-grown cabbage. There was no difference between soil-cultivated Chinese cabbage kimchi and hydroponically cultivated Chinese cabbage kimchi.

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Analysis of Ginsenoside Composition of Woods-grown Ginseng Roots

  • Han, Sung-Tai;Shin, Cha-Gyun;Yang, Byung-Wook;Hahm, Young-Tae;Sohn, Uy-Dong;Im, Byung-Ok;Cho, Soon-Hyun;Lee, Boo-Yong;Ko, Sung-Kwon
    • Food Science and Biotechnology
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    • v.16 no.2
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    • pp.281-284
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    • 2007
  • The objective of this research is to provide basic information necessary to differentiate between ginseng (Panax ginseng) grown in woods environments and cultivated ginseng. The ginseng saponin (ginsenoside) contents of Korean woods-grown, 4 year-old cultivated, and 6 year-old cultivated ginsengs were determined via HPLC analysis. The total saponins in the woods-grown ginseng (0.648%) were approximately twice that of the 4 year-old cultivated (0.270%) and the 6 year-old cultivated ginsengs (0.280%). The protopanaxadiols (PD)/protopanaxatriols (PT) ratio of the woods-grown ginseng (3.258%) was higher than that of the 4 year-old cultivated (2.456%) and the 6 year-old cultivated ginsengs (2.183%). The $Rb_1/Rg_1$ ratio of the woods-grown ginseng (10.225%) was also higher than those of the 4 year-old cultivated (3.514%) and the 6 year-old cultivated ginsengs (4.865%).

Chemical Characteristics and Application for Kraft Pulp of Bed Log Wastes (표고골목의 화학적 특성과 펄프재로서의 이용방안)

  • Jeong, Myung-Joon;Kim, Dae-Young;Jo, Byoung-Muk;Oh, Jung-Soo
    • Journal of the Korean Wood Science and Technology
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    • v.33 no.3 s.131
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    • pp.79-88
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    • 2005
  • This research studied the utilization of the bed log wastes as a papermaking grade pulp. Five different bed log samples from shiitake mushroom (Lentinus edodes (Berk.) Sing) cultivation were collected by the cultivating periods of 1 to 5 years. The wood chemical composition and the characteristics of kraft pulping of each sample were investigated. The results of chemical composition showed that the rate of carbohydrate (glucose and xylose) content in sapwood was decreased as the cultivation period was increased. In heartwood, there was no significant difference. The screening yield of non-cultivated bed log from kraft pulping was higher than that of cultivated one, but the reject of cultivated one, especially for 5 year-cultivated, was lower than non-cultivated bed log. The fiber length and width was continuously decreased as the cultivation period was increased. Therefore, the freeness of the pulp from the cultivated bed log was sharply decreased comparing to non-cultivated due to the fiber cutting and the increased fine content. The dry strengths were increased according to the increasing addition level of bed log kraft pulp to KOCC and non-cultivated wood pulp. From the overall results, the pulp from 5 years cultivated bed log can be reasonably used if it is mixed with long fiber pulp for advantages such as reducing beating time.

Spatial Characteristics and Driving Forces of Cultivated Land Changes by Coupling Spatial Autocorrelation Model and Spatial-temporal Big Data

  • Hua, Wang;Yuxin, Zhu;Mengyu, Wang;Jiqiang, Niu;Xueye, Chen;Yang, Zhang
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.15 no.2
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    • pp.767-785
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    • 2021
  • With the rapid development of information technology, it is now possible to analyze the spatial patterns of cultivated land and its evolution by combining GIS, geostatistical analysis models and spatiotemporal big data for the dynamic monitoring and management of cultivated land resources. The spatial pattern of cultivated land and its evolutionary patterns in Luoyang City, China from 2009 to 2019 were analyzed using spatial autocorrelation and spatial autoregressive models on the basis of GIS technology. It was found that: (1) the area of cultivated land in Luoyang decreased then increased between 2009 and 2019, with an overall increase of 0.43% in 2019 compared to 2009, with cultivated land being dominant in the overall landscape of Luoyang; (2) cultivated land holdings in Luoyang are highly spatially autocorrelated, with the 'high-high'-type area being concentrated in the border area directly north and northeast of Luoyang, while the 'low-low'-type area is concentrated in the south and in the municipal area of Luoyang, and being heavily influenced by topography and urbanization. The expansion determined during the study period mainly took place in the Luoyang City, with most of it being transferred from the 'high-low'-type area; (3) elevation, slope and industrial output values from analysis of the bivariate spatial autocorrelation and spatial autoregressive models of the drivers all had significant effects on the amount of cultivated land holdings, with elevation having a positive effect, and slope and industrial output having a negative effect.

A Study of Recycle of Waste Wood After Cultivating Oak Mushroom (II) - On the Structure of Cellulose Crystal Transformation of the Waste Wood - (표고버섯골목의 재활용에 관한 연구 (II) - 폐골목 세포벽 중의 셀룰로오스 결정의 변태구조 -)

  • Kim, Nam-Hun;Lee, Won-Yang
    • Journal of the Korean Wood Science and Technology
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    • v.23 no.3
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    • pp.1-7
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    • 1995
  • The crystal transformation from cellulose I to cellulose II during alkaline swelling of waste wood, which has been used for cultivating oak mushroom(Cortinellus edodes (Berk.) Ito et Imai), was investigated and compared to that of normal wood by a series of X-ray diffraction analysis. When the sapwood of cultivated wood was treated with 20% NaOH solution for 2 hours, the cellulose I can be easily transformed into Na-cellulose I than normal wood or heartwood of cultivated wood. Certainly the formation of Na-cellulose in wood is proportional to alkali swelling duration, and the formation of cultivated sapwood was faster than that of the other woods. Cellulose I in the sapwood of cultivated wood was easily transformed into cellulose II during mercerization, but the sapwood of normal wood and the heartwood of cultivated wood hardly converted to cellulose II. Namely, most of Na-cellulose I in normal wood can be reconverted to cellulose I in the process of washing and drying. Therefore, it can be concluded from this study that in cell wall lignin and hemicellulose can prevent the alkaline swelling of cellulose in wood and the transformation from cellulose I to cellulose II as well.

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Morphological Variation of Two Cultivated Types of Perilla Crop from Different Areas of China

  • Ma, Shi Jun;Lee, Ju Kyong
    • Horticultural Science & Technology
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    • v.35 no.4
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    • pp.510-522
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    • 2017
  • To better understand the morphological variation for Perilla crop in different areas of China, we studied the morphological variation in 87 accessions (84 cultivated var. frutescens and three cultivated var. crispa) from high latitude (Northeast China) and middle latitude (North and Northwest China) areas of China by examining seven quantitative and 10 qualitative characters. Analysis of the morphological variation determined that there was significant morphological differences in five quantitative traits between cultivated var. frutescens and cultivated var. crispa, including effective number of branches (QN2), number of internodes (QN3), number of branches (QN4), length of the largest inflorescence (QN5), and days from germination to flowering (QN7). However, two quantitative traits-plant height and number of florets of the largest inflorescence-did not show any significant differences between cultivated var. frutescens and cultivated var. crispa. In addition, significant differences for six quantitative traits were found between the accessions of cultivated var. frutescens originating from high and middle latitude areas in China, which included QN2, QN3, QN5, number of florets of the largest inflorescence (QN6), and QN7. Principal components analysis (PCA) identified five quantitative characters [plant height (QN1), QN2, QN3, QN4, QN7] and six qualitative characters [fragrance of plant (QL1), color of reverse side of leaf (QL3), degree of pubescence (QL5), color of flower (QL6), shape of leaf (QL7), and hardness of seed (QL10)] that contributed to the positive direction on the first axis. The other quantitative and qualitative characters contributed to the negative direction on the first axis. Most accessions of cultivated var. frutescens and cultivated var. crispa were clearly separated by the first axis. In addition, most accessions of cultivated var. frutescens are from high latitude and middle latitude areas that were clearly separated by the first axis, except for several accessions. The findings from this study will provide useful information towards understanding the morphological variation of Perilla crop according to geographical distribution in high and middle latitude regions of China.

Analysis of major ginsenosides in various ginseng samples

  • Lee, Dong Gu;Lee, Ju Sung;Kim, Kyung-Tack;Kim, Hyun Young;Lee, Sanghyun
    • Journal of Applied Biological Chemistry
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    • v.62 no.1
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    • pp.87-91
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    • 2019
  • The contents of major ginsenosides (ginsenosides Rb1, ginsenoside Rc, ginsenoside Rd, ginsenoside Re, ginsenoside Rf, and ginsenoside Rg1) in ginseng cultivated in different areas in Korea, ginseng that underwent different cultivation processes and ages, and ginseng cultivated in different countries were determined using high-performance liquid chromatography equipped with UV/VIS detector. Ginsenoside Rc was the most abundant ginsenoside in all different ginseng samples. The highest total concentration of major ginsenosides was found in the ginseng cultivated in Jinan (0.931 mg/g) and 4-year grown red ginseng (1.785 mg/g). Major ginsenosides were the most abundant in Korean ginseng (1.264 mg/g), compared to those in Chinese and American ginseng. The results of this study showed the different contents of major ginsenosides in the ginseng samples tested and emphasized which sample could contain high yield of ginsenosides.

Soil properties of cultivation sites for mountain-cultivated ginseng at local level

  • Kim, Choonsig;Choo, Gap Chul;Cho, Hyun Seo;Lim, Jong Teak
    • Journal of Ginseng Research
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    • v.39 no.1
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    • pp.76-80
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    • 2015
  • Background: Identifying suitable site for growing mountain-cultivated ginseng is a concern for ginseng producers. This study was conducted to evaluate the soil properties of cultivation sites for mountain-cultivated ginseng in Hamyang-gun, which is one of the most well-known areas for mountain-cultivated ginseng in Korea. Methods: The sampling plots from 30 sites were randomly selected on or near the center of the ginseng growing sites in July and August 2009. Soil samples for the soil properties analysis were collected from the top 20 cm at five randomly selected points. Results: Mountain-cultivated ginseng was grown in soils that varied greatly in soil properties on coniferous, mixed, and deciduous broad-leaved stand sites of elevations between > 200mand < 1,000 m. The soil bulk density was higher in Pinus densiflora than in Larix leptolepis stand sites and higher in the < 700-m sites than in > 700-m sites. Soil pH was unaffected by the type of stand sites (pH 4.35-4.55), whereas the high-elevation sites of > 700mwere strongly acidified, with pH 4.19. The organic carbon and total nitrogen content were lower in the P. densiflora stand sites than in the deciduous broad-leaved stand sites. Available phosphorus was low in all of the stand sites. The exchangeable cationwas generally higher in the mixed and low-elevation sites than in the P. densiflora and high-elevation sites, respectively. Conclusion: These results indicate that mountain-cultivated ginseng in Korea is able to grow in very acidic, nutrient-depleted forest soils.