• Journal of Life Science
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• v.26 no.8
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• pp.895-903
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• 2016

This study was carried out to discover the response of cucumber (Cucumis sativus L.) senescence- associated genes (SAGs) to several plant hormones in detached and developing cotyledon. Accordingly, a collection of cucumber SAGs were examined to characterize their gene expression response through semi-quantitative RT-PCR. Cotyledons were excised at day 14 after seed sowing from plantlets, then incubated in 100 μM each of IAA or zeatin solution for up to 4 days in light and darkness. They were collected at 2-day intervals and used for total RNA extraction and subjected to RT-PCR. Gene expression levels of several cucumber SAGs were significantly changed during the incubation period. More than five cucumber SAGs involving SAG 60 responded to the IAA and zeatin treatment. In the ethylene response study, cotyledons were exposed up to 10 days by ethylene gas. Most of the cucumber SAGs did not show immediate response to ethylene in green cotyledon. The exceptions were PCK, SAG 158, and SAG 288 genes, which responded after 1 day of exposure to green cotyledon, while ICL and SAG 281 revealed strong responses after 10 days of being exposed to yellowing cotyledon. These results suggest that several cucumber SAGs react actively in response to starvation or senescence against exogenously applied stimulus. This induced senescence response is able to understand the SAGs role in lipids and amino acids metabolism partly and function in organ senescence during development.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.5
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• pp.272-279
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• 2003

This study was performed to develop a more rapid and simple nutrient diagnosis method of plants than the conventional leaf analysis method. Cucumber (Cucumis sativus L. cv. jangil banbaek) was planted in the mixed media produced by mixing perlite and rock wool at 1:1 (v/v) ratio. The Yamazakki nutrient solution for cucumber was supplied to the media using micro-drip irrigation system. Experimental plots were consisted of no fertilization, deficient fertilization, adequate fertilization, and surplus fertilization for N, P and K. Specific color difference sensor value (SCDSV) measured by chlorophyll meter was closely related to total-N concentration in leaves measured by the conventional method. Nitrate, $PO_4$ and K concentration in petiole sap measured by test strips showed a significant relationship with total-N, P and K concentration in leaves. Linear regression equations between $NO_3$, $PO_4$ and K concentration in petiole sap and total-N, P and K concentration in the leaves were prepared. Optimum levels of $NO_{3}$, $PO_{4}$ and K in petiole sap were obtained by plugging the optimum concentrations of total-N, P and K in the leaves by other researchers into the equations. In conclusion, the SCDSV measured by chlorophyll meter and the concentration of $NO_3$, P and K in petiole sap measured by the test strips would be suitable for rapid estimation of plant nutrient status.

• Research in Plant Disease
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• v.24 no.4
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• pp.328-331
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• 2018

A severe disease with leaf spots and necrotic symptoms was observed in cucumber (Cucumis sativus L.) seedlings in April 2018 at a nursery in Kimjae, Korea (35o 47'09.8"N 127o 2'24.3"E). The infected plants initially showed spots on water-soaked cotyledons which, at later stages, enlarged and spread to the leaves, which the lesions becoming dry and chlorotic. The symptomatic samples were collected from cucumber and the isolates were cultured on LB agar. The representative bacterial strain selected for identification showed fluorescent on King's medium B, was potato rot-positive, levan and arginine dihydrolase-negative, oxidase-negative and tobacco hypersensitivity-positive in LOPAT group 2 as determined by LOPAT tests. A pathogenicity test was carried out on a 3-week-old cucumber. After 3 days of inoculation, leaf spots and necrotic symptoms appeared on the cucumber, similar to the originally infected plants. The infecting bacterial strain was identified as Pseudomonas viridiflava, by 16S rDNA sequence analysis. This is the first report of leaf spot diseases on cucumber caused by P. viridiflava.

• The Plant Pathology Journal
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• v.31 no.4
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• pp.371-378
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• 2015

Surveys of yellowing viruses in plastic tunnels and in open field crops of melon (Cucumis melo cultivar catalupo), oriental melon (C. melo cultivar oriental melon), and cucumber (C. sativus) were carried out in two melon-growing areas in 2014, Korea. Severe yellowing symptoms on older leaves of melon and chlorotic spots on younger leaves of melon were observed in the plastic tunnels. The symptoms were widespread and included initial chlorotic lesions followed by yellowing of whole leaves and thickening of older leaves. RT-PCR analysis using total RNA extracted from diseased leaves did not show any synthesized products for four cucurbit-infecting viruses; Beet pseudo-yellows virus, Cucumber mosaic virus, Cucurbit yellows stunting disorder virus, and Melon necrotic spot virus. Virus identification using RT-PCR showed Cucurbit aphid-borne yellows Virus (CABYV) was largely distributed in melon, oriental melon and cucumber. This result was verified by aphid (Aphis gossypii) transmission of CABYV. The complete coat protein (CP) gene amplified from melon was cloned and sequenced. The CP gene nucleotide and the deduced amino acid sequence comparisons as well as phylogenetic tree analysis of CABYV CPs showed that the CABYV isolates were undivided into subgroups. Although the low incidence of CABYV in infections to cucurbit crops in this survey, CABYV may become an important treat for cucurbit crops in many different regions in Korea, suggesting that CABYV should be taken into account in disease control of cucurbit crops in Korea.

• The Korean Journal of Ecology
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• v.24 no.1
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• pp.67-73
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• 2001

The allelochemicals from aqueous extracts of A. artemisiifolia var. elatior leaves and roots were analyzed and 60 compounds in the leaf and 53 compounds in the roots were inspected. The main compounds were acids,. especially phenolic acids and some non-acids. The total amount of compounds in the aqueous extracts of A. artemisiifolia var. elatior leaves was higher than in the roots. The aqueous extracts had much high inhibiting effects on the germination and seedling elongation of selected plants. In both cases, the inhibiting effects were very different with different selected plants and increased significantly as the concentration of aqueous extracts increased. Capsicum annum, Achyranthes japonica, and Oenothera odorta plants were suffered more significant inhibition effects than Raphnus sativus, Cucumis sativus, Brassica camperstris plants. Aqueous extracts from leaves had slightly higher inhibition effects than the aqueous extracts from the roots.

• Korean Journal of Plant Resources
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• v.26 no.6
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• pp.673-677
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• 2013

In order to examine the effects of Korean cucurbitaceous plants on sonic hedgehog pathway and growth of cancer cells with over-activated hedgehog pathway, we measured the sonic hedgehog conditioned medium (shh-CM) induced alkaline phosphatase (ALP) activity and cell viability of pancreatic cancer cell lines by treatment of cucurbitaceous plants. Among the tested cucurbitaceous plants, Actinostemma lobatum Maxim, Cucumis sativus L., Momordica charantia L., Schizopepon bryoniaefolius Maxim and Trichosanthes kirilowii Max, var. japonica Kitam showed the potent inhibitory effects (> 50 % at $20{\mu}g/mL$) on shh-CM induced ALP activity. We also evaluated the cell viability of pancreatic cancer cells treated with the cucurbitaceous plants. The tested cucurbitaceous plants showed the very weak effects on cancer cell proliferation but, T. kirilowii Max, var. japonica Kitam presented the inhibitory effect of 72.7 % on the proliferation of pancreatic cancer cells at $20{\mu}g/mL$. Taken together, we screened the effects of Korean cucurbitaceous plants on shh-CM induced ALP activity and cell viability of pancreatic cancers to search for the modulators of the hedgehog pathway leading to the inhibition of cancer cell proliferation. T. kirilowii Max, var. japonica Kitam, among the tested cucurbitaceous plants, showed the inhibitory effects on the shh-CM induced ALP activity and the proliferation of pancreatic cancer cells.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2001.11a
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• pp.156-162
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• 2001

최근 겨울동안에 폭설과 강풍과 같은 기상이변이 잦아지면서 기상재해와 함께 정전에 의한 시설 원예작물의 저온피해도 빈번하게 발생하고 있으나, 실제적으로는 어느 정도의 피해가 있는지 파악되지 못하고 있으며 피해 예측에 관한 정보도 축적되어 있지 못한 실정으로 이에 관련된 연구가 요구되고있다. 시설 재배시 겨울철 온도환경 관리에 대한 연구는 주로 난방비 절약을 위한 저온관리 한계온도 구명(Tanaka 등, 1986), 또는 근권부 온도 조절에 의한 작물의 생산성 증진 연구와 같은 실용적 측면의 연구(Lee, 1994; Fujie와 Saidou, 1983) 및 저온 장해의 생리기작(Herner, 1990; Patterson 등, 1976; Reyes와 Jennings, 1994; Wolk와 Herner, 1982; Wright and Simon, 1973)에 관련된 연구가 수행되고 있으며 냉온 피해를 받을 당시의 전후환경조건과 산화작용에 관련된 연구는 토마토(Kerdnaimongkol 등, 1997) 등 몇 개 작물(King 등 1982)에서 보고된바 있다.(중략)

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.1
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• pp.23-27
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• 2014

Nutrient accumulation in surface soil has become a serious problem for cucumber production in greenhouse. However, still in many cases, soil management practices are only focused on maintaining crop yield, regardless of sustainability related with soil chemical properties. This study was conducted to propose a sustainable soil management practice by investigating the impact of cover crop species and nitrogen rate of fertigated solution on cucumber yield and soil chemical properties in greenhouse condition. Rye and hairy vetch were tested as a fallow cover crop, and each amount of urea (1/2, 3/4, 1 times of N fertilizer recommendations), determined by soil testing result, was supplied in fertigation plots as an additional nitrogen source. The result showed that the yield of cucumber was higher in rye treatment than control and hairy vetch treatment. In addition, rye effectively reduced EC and accumulated nutrients from the soil. Meanwhile, N concentration of fertigated solution showed no significant effect on the growth and yield of cucumber. Consequently, these results suggest that it is desirable to choose rye as a fallow catch crop for sustainable cucumber production in greenhouse.

• Horticultural Science & Technology
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• v.17 no.5
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• pp.569-571
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• 1999

The effects of training methods on growth and yield of white spine cucumber (Cucumis sativus L. cv. Sharp-1) were examined. The numbers of nodes, leaf area and root weight were in the order of, from high to low, ateral vine, main plus lateral vine, and main vine training. The total and marketable fruit yields were higher by the lateral training method than those by the other ones. The labor required for training was in the order of, from low to high, lateral vine, main vine, and main plus lateral vine training methods.

• Journal of Bio-Environment Control
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• v.13 no.2
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• pp.96-101
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• 2004

This study was conducted to determine the daily application rate and amount of N and K with fertigation during different growth stages in semi-forcing culture of cucumber plants (Cucumis sativus L. cv. Eunseongbaekdadagi). The diagnostic criteria for N and K also investigated based on petiole sap analysis. The dry weight increased slowly until 30 days after transplanting. The highest dry weights were observed at 60 days after planting, then it decreased. As the plant grew, the contents of N and K in the petiole sap and fruit of cucumber decreased. The daily uptake of N and K were highly correlated with the growing days. The $NO_3$ concentrations in petiole sap were in the range from 3,500 to 4,500 mgㆍ$L^{-1}$ in the early growth stage, but those were in the range from 2,000 to 3,000 mgㆍ$L^{-1}$ after then. However, K concentration in petiole sap were in the rang from 5,000 to 7,000 mgㆍ$L^{-1}$ The fluctuation in petiole sap concentration of K was severe in the monthly fertigation and moderate in the daily fertigation. The fertigation by petiole sap diagnosis forced EC of soil to be low and yield to increase compared to the control.