• Journal of Photoscience
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• v.3 no.1
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• pp.1-7
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• 1996

When cucumber (Cucumis sativus L.) cotyledon discs were floated on $\delta$-aminolevulinic acid, oxyfluorfen, or rose bengal solution under light condition following 20 h dark incubation, rapid electrolyte leakage from the tissues occurred. The electrolyte leakage from the tissues was dependent on the compounds treated, their concentrations, and the duration of light exposure to the tissues. Dark incubation before exposure to continuous white light enhanced electrolyte leakage from the tissues treated with the compounds and reduced lag period for the activity of the compounds. Electrolyte leakage from the treated tissues was greatly influenced by the light intensity to which they were exposed. Higher light intensities stimulated electrolyte leakage and reduced lag period. Porphyrin biosynthesis inhibitors, gabaculine and 4,6-dioxoheptanoic acid, completely inhibited electrolyte leakage from the oxyfluorfen-treated tissues. Protection against the activity of $\delta$-aminolevulinic acid from electrolyte leakage was complete with 4,6-dioxoheptanoic acid, but not with gabaculine. However, gabaculine and 4,6-dioxoheptanoic acid gave no such protection against rose bengal activity. In summary, our results indicate that $\delta$--aminolevulinic acid, oxyfluorfen, and rose bengal exert their effects by causing electrolyte leakage from the treated tissues in a similar manner, except that oxyfluorfen has an apparent lag period for its action on electrolyte leakage increase. All above compounds require preincubation of treated tissues in darkness and subsequent light exposure with a high intensity for their maximal activities. Our results also support that in the presence of light, $\delta$-aminolevulinic acid and oxyfluorfen cause cellular damage through the indirect generation of singlet oxygen from accumulated tetrapyrroles of porphyrin pathway, whereas rose bengal causes cellular damage through the direct generation of singlet oxygen.

• Horticultural Science & Technology
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• v.32 no.2
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• pp.227-234
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• 2014

The responses to chilling temperature of 12 Korean cucumber varieties was compared to those of two U.S.A. (previously determined cold tolerant NC76 and 'Chipper'), and Chinese and Japanese germplasms. Seedlings of each entry were exposed to $4^{\circ}C$ (Experiment 1) and $1^{\circ}C$ (Experiments 2 and 3) at the first-true leaf stage for eight and nine hours, respectively, under 80% relative humidity (RH) and $149{\mu}moles{\cdot}m^{-2}{\cdot}s^{-1}$ photosynthetic photon flux (PPF). The chilling response [damage rating (DR)] of each accession was based on visual ratings (1 to 5) after treatment, where 1 = no damage, 2 = slight, 3 = moderate, 4 = advanced, and 5 = severe damage. Predictably the cumulative average DR of chilling tolerant line NC76 and 'Chipper' after chilling w as 1 and 1.1, respectively. Korean 'Nacdongchungjang' was most sensitive to chilling temperatures [DR = 2.3] when compared to the other entries examined. The sensitivity to chilling of 'Nacdongchungjang' was followed by Chinese 'Dongguan' [DR = 1.7]. In contrast, 'Saeronchungjang' (DR = 1) and 'Janghyungnachap' (DR = 1) were the most chilling tolerant of the Korean accessions examined and equivalent to the response of line NC76 and 'Chipper'. Nevertheless, chloroplast type genotyping of these accessions with known chilling-linked sdCAPS genomic markers revealed genotypic differences between chilling tolerant lines (NC76 and 'Chipper') and all Korean lines examined.

• Korean Journal of Food Science and Technology
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• v.42 no.4
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• pp.407-413
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• 2010

This study was performed to compare the quality and volatile components of two cucumber (Cucumis sativus L.) cultivars (Cheongnakhab, Ipchunakhab)-grown under organic and conventional conditions. The levels of pH and soluble solids in the organic system were similar to those in the conventional system. The hardness of the pulp in the organic cucumbers was slightly higher than that in conventional cucumber regardless of cultivar. However, the contents of chlorophyll a, b, and total chlorophyll in organic cucumbers were significantly (p<0.05) higher than those in conventional cucumber. The major volatile compound of fresh cucumber was (E,Z)-2,6-nonadienal regardless of cultivar and farming system. When classified by the identified components' functional group, the rate of aldehydes was the highest of all samples. The results indicate that the quality and volatile components in organic cucumbers were similar to those in conventional cucumbers except for hardness and chlorophyll contents regardless of cultivar.

• Korean Journal of Soil Science and Fertilizer
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• v.32 no.2
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• pp.171-181
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• 1999

A field experiments was carried out to investigate the physicochemical properties of soil and cucumber growth in vinyl house when irrigation point was made at 0.2, 1/3, 0.5 and 1.0 bar. The obtained results was summarized as follow: The taxonomic class of the soil used was loam and each content of the required water was 4.4, 7.3, 9.6 and 13.4 mm per each irrigation time at 0.2, 1/3, 0.5 and 1.0 bar treatments in spring culture, respectively. At 0.2 bar and 1.0 bar treatments, interval of irrigation was 2.3 and 14.8 day, the times of irrigation was 37 and 6, and total irrigation volume was 163.5 and 80.3 mm, respectively. After cucumber culture, pH, EC concentration and exchangeable K content of soil at 0.2 bar treatment was distributed near to the level of improvement target while EC, available $P_2O_5$ and exchangeable base content in other treatments were higher compared to improvement target. At 1.0 bar treatment, ratios of the solid and liquid phase were 44.9 and 27.1%, respectively, and bulk density was $1.26g\;cm^{-3}$ which was the highest among the treatments. At 0.2 bar treatment, the ratio of the solid and liquid phase was 41.7 and 22.8%, respectively, and bulk density was $1.09g\;cm^{-3}$ which was the lowest. The root length and radius at 0.2 bar treatment were best, while those at 1/3 bar were worst. At 0.2 bar treatment, the total yield was 7,269 kg and the weight of good products was 5,677 kg which was the highest among treatments. At 0.33 bar treatment, the yield was the lowest with the high ratio of deformity.

• Korean Journal of Organic Agriculture
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• v.27 no.1
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• pp.87-100
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• 2019

The study was initiated to compare crop productivity as affected by a long-term application with homemade liquid fertilizers in leading organic cucumber (Cucumis sativus L.) farms in Suncheon and Kimcheon provinces. A Suncheon farm have applied an EM (effective organism) liquid fertilizer for one year and fifteen years, designating as EM 1-year and EM 15-year plots, respectively, with 4-year and 5-year application of native microbes-liquid fertilizer in Kimcheon farm, designating as Micro 4-year and Micro 5-year plots, respectively. pH in the EM-liquid fertilizer was high to approximately 7.7, and EC in the Micro-liquid fertilizer was 0.1 dS/m higher than those of EM-liquid fertilizer, with similar macro-nutrient concentrations observed in the both liquid fertilizers. Soil EC was the highest to the 10.0 dS/m for the liquid fertilizer with EM 1-year and showed less than 1.5 dS/m for other liquid fertilizer plots. Micro-liquid fertilizer plots had soil OM contents less than 20 g/kg, which was approximately two times less than those of EM plots. Soil microbial properties were not significantly different among the liquid fertilizer plots. SPAD and PS II values were significantly increased by EM 15-year plots with high levels of soil OM and EC. Liquid fertilizer plot with EM 1-year had high concentrations of T-N, Ca, and Na in the cucumber crops but low concentrations of P and Mg, in particular for low K of 1.2% which was two times less than those of desired level for an optimum cucumber growth. The lowest fruit yield was observed for the liquid fertilizer plot with EM 1-year with the highest soil EC accumulated. Liquid fertilizer plot with EM 15-year produced the expanded volume of crop canopy and increased fruit yield. Therefore, long-term of continuous application with an organic liquid fertilizer would have sustainably improved soil stability and the crop productivity.

• Journal of Plant Biotechnology
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• v.35 no.4
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• pp.275-280
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• 2008

One of the limitation for Agrobacterium-mediated transformation via organogenesis from cotyledon explants routinely in cucumber is the production of chimeric plants. To overcome the limitation, Agrobacterium-mediated transformation system via somatic embryogenesis from hypocotyl explants of cucumber (c.v., Eunsung) on the selection medium with paromomycin as antibiotics was developed. The hypocotyl explants were inoculated with Agrobacterium tumefaciens strain EHA101 carrying binary vector pPTN290; then were subsequently cultured on the following media: co-cultivation medium for 2 days, selection medium for $5{\times}14$ days, and regeneration medium. The T-DNA of the vector (pPTN290) carried two cassettes, Ubi promoter-gus gene as reporter and 35S promoter-nptll gene conferring resistance to paromomycin as selectable agent. The confirmation of stable transformation and the efficiency of transformation was based on the resistance to paromomycin indicated by the growth of putative transgenic calli on selection medium amended with 100mg/L paromomycin, and GUS gene expression. Forty eight clones (5.2%) with GUS gene expressed of 56 callus clones with resistance to paromomycin were independently obtained from 928 explants inoculated. Of 48 clones, transgenic plants were only regenerated from 5 clones (0.5%) at low frequency. The histochemical GUS assay in the transgenic seeds ($T_1$) also revealed that the gus gene was successfully integrated and segregated into each genome of transgenic cucumber.

• Journal of Bio-Environment Control
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• v.12 no.4
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• pp.173-179
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• 2003

This study was established to control the overgrowth of cucumber and tomato plug seedlings by controlling of irradiation timings light intensities of red (R) light. Compared with the control, end-of-day (EOD) timing of R irradiation significantly reduced the seeding heights by 21.3% and 14.2% in cucumber and tomato, respectively. In addition, both plant seedlings treated with R light at EOD timing had the thickest stem diameter. Tomato seedlings treated with R light at EOD showed th highest chlorophyll content and the smallest leaf size. Dry weights of above ground plant tissue in both plant seedlings were significantly reduced by EOD R light treatment. And both plant seedlings treated with EOD treatment had the lowest T/R ratio, and the highest compactness rates. The increased intensities of R light resulted in the shorter plant heights in cucumber and tomato plant seedlings. It also reduced the length of hypocotyls and internodes and the size of leaves in the both seedlings. Elevated R light intensities tended to increase the stem diameter and chlorophyll contents. Dry weights of above ground plant tissue and roots in both plant seedlings were reduced by the elevated R irradiation. Cucumber seedlings treated with 2 and 8 $mol{\cdot}m^{-2}{\cdot}s^{-1}$ and tomato seedlings treated with 8 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ had significantly lower T/R ratio the other treatments tested.

• Journal of Practical Agriculture & Fisheries Research
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• v.10 no.1
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• pp.77-90
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• 2008

This study was conducted to investigate the influence of chilling temperature and duration at different seeding stages on yield, fruit characteristics and growth of cucumber in a greenhouse. When 20-day-old cucumber were exposed to 0 and 3℃ for 10 hours, 25 and 30% reduction in the main stem elongation rate and 34 and 37% reduction in total leaf area per plant were observed. The reduction in stem elongation and total leaf area was apparently associated with the increases in chilling duration. Exposure of seedlings to 6℃ failed to causes any significant differences in growth as compared to the unchilled plants. Repeated exposure of seedlings to 3℃ chilling for 10 hours per day increased the chilling injury significantly. The seedlings exposed to low temperature for 3 consecutive days exhibited severe injury as compared to the seedlings exposed to chilling treatment only once or twice. Fruit elongation rate was inhibited by approximately 10%, such as 0.59~2.26cm/day, with chilling of 15 hours at 0℃ as compare to 0.61~2.60cm/day in the non-chilled plants. Chilling treatment at 0~3℃ for 10 hours reduced the percentage of marketable fruits by 25~26%. while it increased the percentage of severely bent fruits significantly. Total fruits yield was reduced by 15~25% in cucumber plants when the chilling treatment was given to 20-day-old seedlings and by 22~37% in 30-day-old seedlings. This shows that, Larger seedlings were more sensitive to chilling. Total yield was also influenced by the duration of chilling. Definitely, at 0℃, 5-hour chilling treatment caused 18% of reduction, 10-hour caused 30%, and 15-hour caused 36%, respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.44 no.2
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• pp.194-199
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• 2011

This study was conducted to evaluate fertigation effects of pig slurry (PS) and chemical fertilizer (CF) in cucumber by investigating the growth and yield, nutrient content and uptake, nutrient use efficiency, and soil characteristics in greenhouse cultivation. The cropping patterns of cucumber were semi-forcing culture and retarding culture, and the experiment involves four treatments: No fertilizer (NF), $26mg\;L^{-1}$ and $52mg\;L^{-1}$ of N application by slurry composting biofiltration liquefied fertilizer (SCBLF), and $52mg\;L^{-1}$ of N treatment by chemical fertilizer. The difference on the plant height of cucumber between SCBLF and CF treatments was no significant, but fresh weight and dry weight of stem and root were higher in $26mg\;L^{-1}$ SCBLF treatment. The Ca content of the leaf was lower in the treatments of SCBLF and the K content of the root was lower in the $52mg\;L^{-1}$ CF treatment. The Ca content of the stem was lowest in the $52mg\;L^{-1}$ CF treatment and the mineral content of the fruit showed no significant difference. In case of semi-forcing culture, the gross yield was lower in the $52mg\;L^{-1}$ CF treatment and the yield of unmarketable fruit was lower in the SCBLF treatments. The nutrient use efficiency of cucumber is as follows: K (8.3 ~ 30.9%), N (4.2 ~ 13.0%), P (1.9 ~ 2.0%). The SCBLF treatments showed higher figure in the soil pH than that of the CF treatment, while EC and the content of $NO_3$-N were higher in the CF treatment than the former. The content of exchangeable K was higher in the plot treated with $52mg\;L^{-1}$ SCBLF, and there were no significant differences in the content of Ca and Mg between the treatments. In conclusion, it is suggested that the application of liquefied manure made from pig slurry may be able to replace the use of chemical fertilizer in nitrogen and potassium.

• Horticultural Science & Technology
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• v.31 no.2
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• pp.159-164
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• 2013

A white powder-like substance, so called 'bloom', is formed on the surface of fruits of many varieties of cucumber. Although it is a natural phenomenon, bloomed fruits are accepted lower in quality compared to bloomless fruits by consumers. The experiments were conducted to obtain basic informations for breeding rootstocks, and to develop promising bloomless rootstocks from basic source materials collected and selected by seed company. The surfaces of bloomless fruits were appeared on the bladder cell of glandular trichome and the epicuticular waxes. Those of bloom fruits appeared with an injured bladder cell and many particles, which were not seen on those of bloomless fruits. The chromaticity was investigated on the surface of the bloom and the bloomless fruits. The 'a' and 'b' value of the bloom and the bloomless fruits did not show any significant difference. The 'L' value was significantly different and that of bloom fruits was higher than that of bloomless fruits. Fruit Si content was conspicuously lower in the fruits of cucumber plants grafted on the bloomless rootstock than in the fruits of those grafted on general bloom rootstocks.