• Title/Summary/Keyword: Crystal violet inclusion method

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Effects of Several Seaweed Extracts on the Viability of Human Keratinocyte HaCaT Cells (각종 해조류 추출물들의 인간 피부세포주 HaCaT에 대한 활력 영향)

  • Yoon, Seung-Je;Cho, Yeon-Sook;Nam, Ju-Hyun;Lee, Hyung-Ho;Kim, Eliya;Hong, Yong-Ki
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.41 no.1
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    • pp.68-72
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    • 2008
  • The cellular viability of the human keratinocyte cell line HaCaT was compared after adding seaweed extracts to the culture medium. The viability was measured using a quick, quantitative, spectrophotometric crystal violet inclusion method. Of 36 common seaweed species tested, methanol extracts from Sargassum sagamianum and Gigartina tenella enhanced the viability of HaCaT cells by 1.6-fold, as compared to control cells, while methanol extracts from Dictyota dichotoma, Pachymeniopsis elliptica, and Enteromorpha linza decreased the viability to less than half that of controls.