DOI QR코드

DOI QR Code

Effects of Several Seaweed Extracts on the Viability of Human Keratinocyte HaCaT Cells

각종 해조류 추출물들의 인간 피부세포주 HaCaT에 대한 활력 영향

  • Yoon, Seung-Je (Department of Biotechnology and Institute of Fisheries Sciences, Pukyong National University) ;
  • Cho, Yeon-Sook (School of Food Science and Food Service Industry, Yeungnam University) ;
  • Nam, Ju-Hyun (Division of Food Nutrition and Culinary Arts, Daegu Technical College) ;
  • Lee, Hyung-Ho (Department of Biotechnology and Institute of Fisheries Sciences, Pukyong National University) ;
  • Kim, Eliya (Fisheries and Oceans Canada, Pacific Biological Station) ;
  • Hong, Yong-Ki (Department of Biotechnology and Institute of Fisheries Sciences, Pukyong National University)
  • 윤승제 (부경대학교 생물공학과 및 수산과학연구소) ;
  • 조연숙 (영남대학교 식품외식학부) ;
  • 남주현 (대구공업대학 식품영양조리과) ;
  • 이형호 (부경대학교 생물공학과 및 수산과학연구소) ;
  • ;
  • 홍용기 (부경대학교 생물공학과 및 수산과학연구소)
  • Published : 2008.02.29

Abstract

The cellular viability of the human keratinocyte cell line HaCaT was compared after adding seaweed extracts to the culture medium. The viability was measured using a quick, quantitative, spectrophotometric crystal violet inclusion method. Of 36 common seaweed species tested, methanol extracts from Sargassum sagamianum and Gigartina tenella enhanced the viability of HaCaT cells by 1.6-fold, as compared to control cells, while methanol extracts from Dictyota dichotoma, Pachymeniopsis elliptica, and Enteromorpha linza decreased the viability to less than half that of controls.

Keywords

References

  1. Ahmed, S.A., R.M. Gogal Jr. and J.E. Walsh. 1994. A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: An alternative to H3-thymine incorporation assay. J. Immunol. Methods, 170, 211-224 https://doi.org/10.1016/0022-1759(94)90396-4
  2. Ali, M.S. and M.K. Pervez. 2003. Ring-A hydroxylated dolastanes from the marine brown alga Dictyota dichotoma (Huds.) Lamour. Nat. Prod. Res., 17, 281-286 https://doi.org/10.1080/1057563031000072514
  3. Ali, M.S., M.K. Pervez, F. Ahmed and M.Saleem. 2004. Dichotenol-A, B and C: The C-16 oxidized secodolastanes from the marine brown alga Dictyota dichotoma (Huds.) Lamour. Nat. Prod. Res., 18, 543-549 https://doi.org/10.1080/14786410310001622059
  4. Alonso-Lebrero, J.L., C. Dominguez-Jimenez, R. Tejedor, A. Brieva and J.P. Pivel. 2003. Photoprotective properties of a hydrophilic extract of the fern Polypodium leucotomoson human skin cells. J. Photochem. Photobiol., 70, 31-37 https://doi.org/10.1016/S1011-1344(03)00051-4
  5. Berry, M.N., A.M. Edwards and G.J. Barritt. 1991. Isolated hepatocytes. Preparation, Properties and Applications. Elsevier, New York, 1-47
  6. Cannell, J.P.R. 1993. Algae as a source of biologically active products. Pestie Sci., 39, 147-153 https://doi.org/10.1002/ps.2780390208
  7. Denizot, F. and R. Lang. 1986. Rapid colorimetric assay for cell growth and survival. J. Immunol. Methods, 89, 271-277 https://doi.org/10.1016/0022-1759(86)90368-6
  8. Deters, A.M., C. Lengsfeld and A. Hensel. 2005. Oligo- and polysaccharides exhibit a structure-dependent bioactivity on human keratinocytes in vitro. J. Ethnopharm., 102, 391-399 https://doi.org/10.1016/j.jep.2005.06.038
  9. Do, J.R., Y.J. Nam, J.H. Park and J.H. Jo. 1997. Studies on chemical composition of red algae. J. Kor. Fish. Soc., 30, 428-431
  10. Donguibogam Committee. 1999. Translated Donguibogam. Bubinmunwha Press, Seoul, 1-2198
  11. Hecker, H., R. Johannisson, E. Koch and C.P. Siegers. 2002. In vitro evaluation of the cytotoxic potential of alkylphenols from Ginkgo biloba L. Toxicology, 177, 167-177 https://doi.org/10.1016/S0300-483X(02)00189-0
  12. Indegaard, M. and K. Ostgaard. 1991. Polysaccharides for food and pharmaceutical uses. In: Seaweed Resources in Europe: Uses and Potential. Guiry, M.D. and G. Blunden, eds. Wiley & Sons Ltd., 169-183
  13. Ito, M. 1984. Microassay for studying anticellular effects of human interferon. J. Interf. Res., 4, 603-608 https://doi.org/10.1089/jir.1984.4.603
  14. Jin, Q., S. Dong and C. Wang. 2005. Allelopathic growth inhibition of Prorocentrum micans (Dinophyta) by Ulva pertusa and Ulva linza (Chlorophyta) in laboratory cultures. Eur. J. Phycol., 40, 31-37 https://doi.org/10.1080/09670260400019741
  15. Lincoln, A.R., K. Strupinski and M.J. Walker. 1991. Bioactive compounds from algae. Life Chem. Rep., 8, 97-183
  16. Matsuzaki, S. and K. Iwamura. 1980. Application of seaweeds to human nutrition and medicine. In: Proceedings of the International Symposium for Food from the Sea. Noell, H., ed. Bremerhaven, Germany, 162-185
  17. Park, J.C., Y.I. Jang, M.S. Doo, S.H. Kim and J.W. Choi. 1996. Effect of methanolic extract of Pachymeniopsis ellipticaon lipids components of hyperlipidemic rats. J. Kor. Soc. Food Sci. Nutr., 25, 958-962
  18. Sanchez, L., M. Mitjans, M.R. Infante and M.P. Vinardell. 2006. Potential irritation of lysine derivative surfactants by hemolysis and HaCaT cell viability. Toxicol. Lett., 161, 53-60 https://doi.org/10.1016/j.toxlet.2005.07.015
  19. Smit, A.J. 2004. Medicinal and pharmaceutical uses of seaweed natural products: A review. J. Appl. Phycol., 16, 245-262 https://doi.org/10.1023/B:JAPH.0000047783.36600.ef
  20. Suh, K.H., K.H. Ahn, H.S. Lee, H.G. Lee, J.K. Cho and Y.K. Hong. 1999. Biosorption of Pb and Cr by using Sargassum sagamianum. J. Kor. Fish. Soc., 32, 399-403
  21. Tseng, C.K. and C.F. Chang. 1984. Chinese seaweeds in herbal medicine. Hydrobiologia, 116/117, 152-154
  22. Yoon, J.A., K.W. Yu, W.J. Jun, H.Y Cho, Y.S. Son and H.C. Yang. 2000. Purification of blood anticoagulant polysaccharide from Pachymeniopsis elliptica. J. Kor. Soc. Food Sci. Nutr., 29, 908-916

Cited by

  1. 우수한 탈취율과 항산화능을 갖는 다시마 추출물의 규명 vol.31, pp.3, 2008, https://doi.org/10.5764/tcf.2019.31.3.195