• Title/Summary/Keyword: Crystal violet

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Characteristics of Potato Common Scab Pathogens from Continuous Cropping Fields in Korea (국내감자 연작지대에서 분리한 더뎅이병원균의 특성)

  • 김주희;이왕휴
    • Korean Journal Plant Pathology
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    • v.12 no.1
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    • pp.109-115
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    • 1996
  • 국내 감자 연작 재배지에서 수집하여 분리동정한 더뎅이병원균인 Streptomyces scabies의 배양적, 형태적, 생리적 특성을 조사한 결과는 다음과 같다. 이병감자에서 분리된 균들은 병원성 균주와 비병원성 균주들로 구분되었고, 이들 간에는 뚜렷한 차이를 보였는데, 전형적인 병징을 나타내는 병원성균주는 비병원성균주와는 달리, 나선형의 포자사슬, 회색 포자, 멜라닌 색소를 생성하고 D-glucose, L-arabinose, D-fructose, D0mannitol, raffinose, rhamnose, sucrose, i-inositol, D-xylose 등의 탄소원을 이용하였으며, 또한 7% NaCl 및 streptomycin sulfate, crystal violet, olean domycin(10$\mu\textrm{g}$/ml, 100$\mu\textrm{g}$/ml)등의 항생물질에 감수성을 나타내었다.

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Potato Common Scab by Streptomyces turgidiscabies (Streptomyces turgidiscabies에 의한 감자 더뎅이병)

  • 김전순;박덕환;임춘근;최용철;함영일;조원대
    • Korean Journal Plant Pathology
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    • v.14 no.5
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    • pp.551-554
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    • 1998
  • Bacterial specs isolated from common scab lesion on potato (Solanum tuberosum L. c.. Dejima) tuber was identified as Streptomyces turgidiscabies. This organism had flexuous spore chains and grey spore mass color, produced melanin pigment on ISP 7, but did not produce on ISP 6. S. turgidiscabies grew on agar media at pH 4.5, used L-arabinose, D-fructose, D-glucose, D-mannitol, raffinose, rhamnose, sucrose, D-xylose and meso-inositol as carbon sources, and was susceptible to 7% NaCl, thallium acetate (10 $\mu\textrm{g}$/ml, 100$\mu\textrm{g}$/ml), crystal violet (0.5 $\mu\textrm{g}$/ml), phenol (0.1%, wt/vol), oleandomycin (100 $\mu\textrm{g}$/ml), and streptomycin (20 $\mu\textrm{g}$/ml).

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Potato Scab Caused by Streptomyces acidiscabies (Streptomyces acidiscabies에 의한 감자 더뎅이병)

  • 김점순;박덕환;최용철;임춘근;홍순영;이승돈;함영일;조원대
    • Korean Journal Plant Pathology
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    • v.14 no.6
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    • pp.689-692
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    • 1998
  • Bacterial species isolated from common scab lesion on potato (Solanum tuberosum L. cv. Dejima) was identified as Streptomyces acidiscabies. This organism had flexuous spore chains and white spore mass color, produced melanin pigment on tyrosine agar medium but did not produce on peptone agar medium. S. acidiscabies grew on agar medium at pH 4.0, used L-arabinose, D-fructose, D-glucose, D-mannitol, rhamnose, sucrose, D-xylose and meso-inositol except reffinose as carbon sources. It was also susceptible to thallium acetate (10 $\mu\textrm{g}$/ml, 100$\mu\textrm{g}$/ml), phenol (0.1%, wt/vol), streptomycin (20 $\mu\textrm{g}$/ml), and was resistant to 7% NaCl, crystal violet (0.5 $\mu\textrm{g}$/ml), penicillin (10 IU/ml) and oleandomycin (25 $\mu\textrm{g}$/ml, 100 $\mu\textrm{g}$/ml).

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Biofilm-forming ability and adherence to poly-(methyl-methacrylate) acrylic resin materials of oral Candida albicans strains isolated from HIV positive subjects

  • Uzunoglu, Emel;Bicer, Arzu Zeynep Yildirim;Dolapci, Istar;Dogan, Arife
    • The Journal of Advanced Prosthodontics
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    • v.6 no.1
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    • pp.30-34
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    • 2014
  • PURPOSE. This study evaluated the adhesion to acrylic resin specimens and biofilm formation capability of Candida albicans strains isolated from HIV positive subjects' oral rinse solutions. MATERIALS AND METHODS. The material tested was a heat-cured acrylic resin (Acron Duo). Using the adhesion and crystal violet assays, 14 oral Candida albicans isolated from HIV-positive subjects and 2 references Candida strains (C. albicans ATCC 90028 and C. albicans ATCC 90128) were compared for their biofilm production and adhesion properties to acrylic surfaces in vitro. RESULTS. There were no significant differences in adhesion (P=.52) and biofilm formation assays (P=.42) by statistical analysis with Mann-Whitney test. CONCLUSION. Denture stomatitis and increased prevalence of candidal carriage in HIV infected patients is unlikely to be related to the biofilm formation and adhesion abilities of C. albicans to acrylic resin materials.

Acridine Orange-induced Changes in Cell Wall of Salmonella pullorum (Acridine Orange에 의한 Salmonella pullorum의 세포벽 변화)

  • Kim, Jong-bae;Mah, Jum-sool
    • Korean Journal of Veterinary Research
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    • v.25 no.2
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    • pp.149-153
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    • 1985
  • Salmonella pullorum strain W was serially passaged on the brain heart infusion agar containing acrdine orange(AO) as a concentration of 100 mcg/ml. S. pullorum AO60 and S. pullorum AO150, which were subcultured 60 and 150 passages on AO media, were examined for permeability barrier function of the cell wall. AO60 and AO150 were appeared to be decreased in susceptibility against hydrophobic substances such as crystal violet, chloramphenicol and rifamycin, which might be resulted from the changes of permeability barrier function of the cell wall. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis of bacterial protein, the protein profiles of AO6O and AO150 didn't differ significantly from W, but increased amount of the band of MW 140,000-145,000 was confirmed. And [G+C] contents of DNA in AO60 and A0150 were decreased than that of W.

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Image-Enhanced Endoscopy in Lower Gastrointestinal Diseases: Present and Future

  • Lee, Han Hee;Lee, Bo-In
    • Clinical Endoscopy
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    • v.51 no.6
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    • pp.534-540
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    • 2018
  • From dye-assisted conventional chromoendoscopy to novel virtual chromoendoscopy, image-enhanced endoscopy (IEE) is continuously evolving to meet clinical needs and improve the quality of colonoscopy. Dye-assisted chromoendoscopy using indigo carmine or crystal violet, although slightly old-fashioned, is still useful to emphasize the pit patterns of the colonic mucosa and predict the histological structures of relevant lesions. Equipment-based virtual chromoendoscopy has the advantage of being relatively easy to use. There are several types of virtual chromoendoscopy that vary depending on the manufacturer and operating principle. IEE plays distinctive roles with respect to histologic characterization of colorectal polyps and prediction of the invasion depth of colorectal cancers. In addition, the newest models of IEE have the potential to increase adenoma and polyp detection rates in screening colonoscopy.

Compact, Wavelength-selectable, Energy-ratio Variable Nd:YAG Laser at Mid-ultraviolet for Chemical Warfare Agent Detection

  • Kim, Jae-Ihn;Cho, Ki Ho;Lee, Jae-Hwan;Ha, Yeon-Chul
    • Current Optics and Photonics
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    • v.3 no.3
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    • pp.243-247
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    • 2019
  • We have developed a compact, wavelength-selectable, Q-switched Nd:YAG laser at mid ultraviolet for chemical warfare agent detection. The fundamental wave at 1064 nm is delivered by a pulsed solid state laser incorporating with a square-type Nd:YAG rod in a resonator closed by two crossed Porro prisms for environmental reliability. The output energy at 213 nm ($5{\omega}$) and 266 nm ($4{\omega}$) by ${\chi}^{(2)}$ process in the sequentially disposed BBO crystals are measured to be 6.8 mJ and 15.1 mJ, respectively. The output wavelength is selected for $5{\omega}$ and $4{\omega}$ by a motorized wavelength switch. The energy ratio of the $5{\omega}$ to the $4{\omega}$ is varied from 0.05 to 0.85 by controlling the phase matching temperature of the nonlinear crystal for sum-frequency generation without change of the output pulse parameters.

In vitro efficacy of N-acetylcysteine in combination with antimicrobial agents against Pseudomonas aeruginosa in canine otitis externa

  • Son, Youngmin;Bae, Seulgi
    • Korean Journal of Veterinary Research
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    • v.61 no.2
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    • pp.16.1-16.6
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    • 2021
  • Pseudomonas aeruginosa is one of the most common pathogenic species associated with canine otitis externa (OE). Their resilience is achieved by forming a biofilm, which allows these bacteria to evade even the harshest of treatments. This study evaluated the in vitro synergistic efficacy of N-acetylcysteine (NAC) with different antimicrobial agents against P. aeruginosa isolated from dogs with OE to develop an effective treatment against P. aeruginosa. The antimicrobial activity was evaluated by the minimum inhibitory concentration test using the microdilution method. The efficacy of antibiofilm formation was evaluated using a crystal violet stain method. The treatment solutions included NAC alone, and in synergy with enrofloxacin, polymyxin B, and gentamicin. NAC alone exhibited antimicrobial and antibiofilm abilities. On the other hand, the combination of NAC and the antibiotics did not show any significant synergistic effects against P. aeruginosa.

Physico-chemical Characteristics of the Inhibitory Substance on the Germination of Mung Bean (녹두 발아 조해물질(阻害物質)의 이화학적(理化學的) 성질(性質))

  • Kim, Kwang-Hyeon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.14 no.1
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    • pp.57-60
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    • 1985
  • The inhibitory substance on the growth of mung bean seedling was amorphous crystal that had a single spot on silica gel thin layer chromatography under ultra-violet irradiation in dark room. The Rf value was 0.85 in n-butanol-acetic acid-formic acid-water(15 : 12 : 3 : 10) system, and 0.92 in n-butanol-acetic acid-water(4 : 1 : 5) system, respectively. Biochemical reaction of the inhibitor was negative to $FeCl_3$, pyrimidine, ninhydrine, folin, fehling, Salkowski and Ehrlich reaction, but the inhibitor had a blue fluorescence from irradiation of ultra-violet rays and had maximum absorption at 252nm on UV spectrum. On IR spectrum, the Peaks of the inhibitor appeared at $3300{\sim}3500,\;2900{\sim}3000,\;1600{\sim}1700,\;and\;1400cm^{(-1)}$. The strain tested was identified to be similar Streptomyces iuteogriseus by morphorogical and physiological characteristics.

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THE EFFECT OF STATIC MAGNETIC FIELDS ON MOLECULAR AND CELLULAR ACTIVITIES (정자기장이 효소와 세포 활성에 미치는 영향)

  • Park, Jae-Gu;Hwang, Hyeon-Shik
    • The korean journal of orthodontics
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    • v.27 no.6 s.65
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    • pp.929-941
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    • 1997
  • Optimal force for orthodontic treatment is the force that produces a rapid rate of tooth movement without discomfort to the Patient or ensuing tissue damage. Recently considerable interest has been generated in the application of magnets as a way to obtain an optimal force. The purpose of the present study was to investigate the effect of static magnetic fields of Sm-Co magnets on molecular and cellular activities. The distance of erythrocyte sedimentation was measured directly, and the activities and the syntheses of $Fe^{2+}$-related enzymes (catalase and NO synthase) and non $Fe^{2+}$-related enzyme (lactic dehydrogenase) were assayed by the spectrophotometer. The growth and the proliferation of osteoblast-like cells $MC_3T_3-E_1$ were determined by the crystal violet staining and the ${^3}H$-thymidine incorporation. The erythrocytes were exposed to the pole face flux density of 1,400 G (gauss), and the enzymes and osteoblast-like cells $MC_{3}T_3-E_1$ were exposed to the flux density of 7,000 G. The results obtained were as follows: 1. The distance of sedimentation of erythrocyte was not affected by the static magnetic fields. 2. The activities of catalase and lactic dehydrogenase were not affected by the static magnetic fields. 3. The intracellular syntheses of NO synthase and lactic dehydrogenase were not affected by the static magnetic fields. 4. The growth and the proliferation of cultured osteoblast-like cells $MC_{3}T_3-E_1$ were not affected by the static magnetic fields. These results suggested that the molecular and cellular activities were not significantly influenced by the static magnetic fields.

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