• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.854-858
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• 2005

Methanol and water crude extracts, crude polysaccharides and crude polysaccharides-free of water crude extracts from Shiitake mush-room (Lentinus edodes) were investigated for their antioxidant capacity in total polyphenolics and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Total polyphenolics in the crude polysaccharides-free of water crude extracts were higher than that of the other ones. And crude polysaccharides-free of water crude extracts and water crude extracts show high on DPPH free radical scavenging activity. Positive correlations were found between total polyphenolics and their antioxidant activity.

• Journal of Ginseng Research
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• 제17권3호
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• pp.196-202
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• 1993

A study was performed to compare the anticancer effects of Korean and Chinese red ginseng roots. The whole crude extracts or chloroform, methanol and acetone fractions of the crude extracts were added in the culture medium of three cancer cell lines, a mouse leukemia cell line ($P_{388}$), a human colon carcinoma cell line (HT-29) and a human rectal carcinoma cell line (HRT-18), to screen the growth inhibition effects. The results are summarized as follows : 1. Crude extracts of both Korean and Chinese red ginseng roots inhibited the proliferation of all the three cancer cell lines tested in a dose dependent manner. However, the growth inhibition effects of Korean red ginseng extracts were significantly greater than that of Chinese red ginseng. 2. An acetone fraction showed the greatest antiproliferative effects among the 11'hole crude extracts, chloroform, methanol and acetone fractions of the crude extracts. 3. These results suggest that the active antiproliferative components of the crude extracts are present mostly in the acetone fraction.

• Journal of Ginseng Research
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• 제15권3호
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• pp.192-196
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• 1991

As a part of studios on the Quality control of index components in crude drug preparations, extraction yields of ginseng saponins from crude drug extracts were identified by TLC and quantified by HPLC. So-Shi-Ho-Tang(小柴胡湯), Sa-Kun-Ja-Tang(四君子湯), Yook-Kun-Ja-Tang(六君子湯) and In-sam-Tang(人蔘湯) were extracted with water, 30%-ethanol, 50%-ethanol, 80%-ethanol and absolute ethanol to analyze ginseng saponins in the crude drug extracts prepared with various concentrations of ethanol. Ginseng saponins were extracted considerably more from the extracts with higher concentrations of ethanol than those with water or lower concentrations of ethanol. Extraction yields of ginseng-side-Rb$_1$, -Rb$_2$ and -R$_c$ from four crude drug preparations were the lowest as 4.9~45.9%, 5.0~40.1, and 6.3~43.7% in water extract and the highest as 29.5~62.6%, 26.7~61.4% and 31.4~62.0% in absolute ethanol extract, compared with those of 80%-methanol extracts.

• Archives of Pharmacal Research
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• 제19권6호
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• pp.456-461
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• 1996

Vascular relaxation action of crude extracts of two kinds of Coptidis rhizoma (Coptis chinensis and Coptis japonica, Ranunculaceae) was investigated and compared with that of berberine and palmatine, active alkaloid components of these plants. The results show that total extracts, berberine, and palmatine induced a concentration-dependent vasodilatation of rat thoracic aorta contracted with phenylephrine (PE). Palmatine, unlike to berberine, did not inhibit contraction induced by KCI. In calcium-free media, not only berberine but also crude extracts inhibited calcium-induced contraction. Furthermore, pretreatment of crude extracts inhibited contraction induced by PE noncompetitively. In PE-induced contraction, berberine was 2.5 times more potent than Coptis chinensis in the relaxation of rat aorta in terms of $IC_{50}$ values. Analysis of the effects of crude extracts on the Emax and $IC_{50}(PE)IC_{50}(KCI)$ ratios provides information on selectivity and indicates that extracts exhibit greater inhibition of the contrac tile response induced by PE than by KCI. We concluded that crude extracts have .alpha.-adrenoceptor blocking action and possesses inhibitory effect on calcium influx, which may be at least in part responsible for the antihypertensive action.

• 한국자원식물학회지
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• 제10권4호
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• pp.351-359
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• 1997

3가지의 식물체 추출액을 공시하여 P. capsici에 대한 항균 활성 검정, 고추 역병에 대한 방제효과 및 고추에 미치는 약해를 조사한 결과는 다음과 같다. 1. 3가지 식물체 물 추출액 중에서 일황련 추출애기 균새장 억제효과가 가장 뛰어났으며, 2배의 희석농도에서 70% 이상의 억제 효과가 있었고, methanol 추출액의 경우 1,000배 이상의 희석농도에서도 74.4%의 억제 효과가 있었다. 지모 및 일황련 추출액의 2개 희석농도와 황백나무의 추출원액 처리에 의해 P.capsici의 유주자낭의 발아는 완전히 억제되었다. 2. Methanol에 의한 추출액이 상온에서 물에 의해 추출하거나, 끓여서 추출한 액보다 항균력이 우수하였다. 3. 세 가지 식물체 추출액 모두 저장기간이 길어짐에 따라 고추 역병균에 대한 항균력은 감소하는 경향을 보였다. 4. 세 가지 추출액 모두 고추 역병에 대해서 방제효과가 있었고, 그 중 일황련 추출액이 가자 효과적이며 황백나무 및 지모의 순이었다. 5. 수경 재배형식의 유모검정에서는 고추묘에 약해가 있었으나, pot시험에서는 약해가 없었다. 또한 고추 종자에 추출액을 처리했을 때 발아가 저해되거나 유근의 신장이 억제되는 등 약해가 유발되어쓰나, 고추 잎과 고추 과실에 추출애글 엽면살포 하였을 때에는 아무런 해가 나타나지 않았다.

• 생약학회지
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• 제26권4호
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• pp.355-359
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• 1995

To find serotonin(5-hydroxytryptamine, 5-HT)-antagonizing activities in traditional herbal drugs, crude extracts from 66 kinds of traditional herbal drugs were randomly screened for inhibitory effects on 5-hydroxytryptophan(HTP)-induced diarrhea in mice. Intraperitoneal injection of 5-HTP(2.5 mg/kg) induced diarrhea in 92% of mice, when observed from 10 to 15 min after injection. Crude extracts(2 g/kg) from 66 kinds of traditional herbal drugs were orally pretreated for 1 h before 5-HTP injection. Of the 66 herbal drugs screened, Ephedrae Herba(麻黃), Cimicifugae Rhizoma(升麻), Anisi stellati Fructus(八角茴香), Aurantii Fructus(枳實), Polygalae Radix(遠志) showed the most potent inhibiting activities against 5-HTP(2.5 mg/kg)-induced diarrhea in mice. There are at least 3 possible mechanisms that would be responsible for the inhibitory effect of crude extracts on 5-HTP-induced diarrhea; 1) crude extract-induced inhibition of the activity of aromatic aminoacid decarboxylase catalyzing the conversion of 5-HTP to 5-HT, 2) crude extract-induced blockade of 5-HT receptor(s) in the gastrointestinal tract responsible for 5-HTP-induced diarrhea, 3) crude extract-induced inhibition of gastrointestinal activity, irrespective of 5-HT system. The exact mechanisms and molecules, responsible for the inhibitory effect of crude extracts on 5-HTP-induced diarrhea remain to be clarified.

• 한국식품조리과학회지
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• 제22권4호통권94호
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• pp.514-520
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• 2006

The quality characteristics of doenjang which contained Astragalus membranaceus water extracts were investigated in this study. The contents of moisture, crude protein, and crude fat were not significantly different in the doenjang which contained Astragalus membranaceus water extracts. The crude ash content was significantly higher in the doenjang which contained Astragalus membranaceus water extracts. The doenjang which contained Astragalus membranaceus water extracts showed higher a-value. Antioxidative activity was evaluated by using the thiobarbituric acid reaction products assay and peroxide value. Antioxidative activity and hydrogen donating activity were increased in doenjang which contained Astragalus membranaceus water extracts. Doenjang which contained Astragalus membranaceus water extracts showed no differences with traditional doenjang in sensory profiles of taste, color, flavor, and overall quality. These result showed the applicability and higher quality of doenjang which contained Astragalus membranaceus water extracts.

• Biomolecules & Therapeutics
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• 제12권2호
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• pp.79-84
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• 2004

02PS15 extracts (BuOH, $H_2O$, and crude extracts) significantly inhibited IL-4 and IL-6 secretion from the phytohemagglutinin (PHA)-plus phorbol 12-myristate 13-acetate (PMA)-induced peripheral blood mononuclear cleas (P<0.05). 02PS15 extracts (BuOH and crude extracts) also significantly inhibited the histamine release from rat peritoneal mast cells (P<0.05). Significant reduced levels (P<0.05) of PMA- and A23187-induced IL-8 were observed in the human mast cell line, HMC-1, with O2PS15 extracts (BuOH and crude extracts). 02PS15 extracts (BuOH and crude extract) downregulated the expression of IL-6 and IL-8 in the activated HMC-1. These results suggest that O2PS15 has the inhibitory effect of atopic allergic reaction anil this might be useful for clinical application to treat several allergic diseases such as atopic dermatitis.

• 한국응용과학기술학회지
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• 제31권1호
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• pp.130-135
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• 2014

천연에서 유래한 항산화 및 항용혈 소재개발을 위해 복분자 에탄올 추출물의 생리활성 효능 평가를 실시하였다. 복분자를 에탄올 추출한 후, 획득한 추출물을 섬유아세포주인 HS68에 처리하여 DPPH 라디칼 소거능과 SOD 유사 항산화 효능을 평가하였다. 또한 Sprague Dawley 렛트 적혈구 세포에 과산화수소로 산화를 유도한 후 추출물의 항용혈 효능을 살펴보았다. 그 결과 추출물은 농도 의존적으로 DPPH 라디칼 소거능과 SOD 유사 활성이 증가하는 것으로 나타났다. 렛트 적혈구를 이용한 항용혈 평가에서는 과산화수소를 단독으로 처리한 양성대조군보다 추출물을 처리한 군에서 항용혈율이 농도 의존적으로 유의하게 증가하였다. 이들 결과는 복분자 추출물이 항산화효능과 항용혈 약용 식물 소재로서의 가능성을 내는 것으로 사료된다.

• 생약학회지
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• 제2권4호
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• pp.177-179
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• 1971

The study on cytotoxicities of domestic antitumour crude drugs were carried out in order to evaluate the antitumour activity. The eleven crude drugs were studied in this paper. No cytotoxicities were observed both at 0.1ml of water extracts and alcohol extracts deuted against monkey kidney cell and HeLa-cell after 3 days cultivation at $37^{\circ}C$. The sample shown the heavy cytotoxicities against monkey kidney cell at 0.3ml alcohol extracts diluted sample solution are Lonicerae Flos and Puchrestae Radix.