• Journal of Physiology & Pathology in Korean Medicine
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• v.20 no.1
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• pp.193-195
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• 2006

The effects of ethanol extract from Lepidolaena clavigera (L. clavigera) on antifungal activity were investigated. The crude ethanol extract of L. clavigera inhibited the growth of the Gram positive bacterium Bacillus subtilis ATCC 19659, (1 mm inhibition zone at 150 ${\mu}g/disc$) and the dermatophytic fungus Trichophyon mentagrophyes ATCC 28185, (2 mm inhibition zone at 150 ${\mu}g/disc$), and cytotoxic to P388 murine leukaemia cells ATCC CCL 46 P388D1, (IC50 >12,500 ${\mu}g/mL\;at\;150\;{\mu}g/disc$) and cytotoxic to BSC monkey kidney cells (@ 5 mg/mL, 150 ${\mu}g/disc$; ++: 50% activity). We suppose that this crude ethanol extract of L. clavigera is the antifungal activity.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.2
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• pp.511-514
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• 2005

The effects of chloroform extract from Riccardia marginata on antifungal activity were investigated. The crude chloroform extract of R. marginata inhibited the growth of the Gram positive bacterium Bacillus subtilis ATCC 19659, (4 mm inhibition zone at $150\;{\mu}g/disc$) and the dermatophytic fungus Trichophyton mentagrophytes ATCC 28185, (6 mm inhibition zone at $150\;{\mu}g/disc$), and inactive to P388 murine leukaemia cells ATCC CCL 46 P388D1, ($IC_{50}\;>25,000\;{\mu}g/mL$ at $150\;{\mu}g/disc$). This crude chloroform extract of R. marginata showed strong antifungal activity against the dermatophytic fungus Trichophyton mentagrophytes.

• Korean Journal of Medicinal Crop Science
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• v.9 no.1
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• pp.45-54
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• 2001

The biological activities of ethanol, ethanol: water(1 : 1v/v) and water extracts from Glycyrrhiza uralensis Fisch, glycyrrhizin and enzymatically hydrolyzed glycyrrhizin were compared. About 50% of the growth of MCF7, A549, Hep3B and AGS cells were inhibited in adding 1.0 g/L of the crude extracts, glycyrrhizin and enzymatically hydrolyzed glycyrrhizin. For example, the ethanol extract inhibited 76%, 66% in MCF7 and Hep3B cells by adding 1.0 g/L. For cytotoxicity on human normal liver cell(WRL-68), the crude extracts were scored as above 26%. For the result of antimutagenecity using CHO V79 cell, the crude extracts proved more effective than other samples. The growth of human immune B and T cells were enhanced up to $1.2{\sim}1.3$ times by adding the crude extracts. In inhibitory effect of ${\alpha}-glucosidase$ activity was showed that the ethanol extract, water extract and ethanol: water (1 : 1v/v) extract were appeared 65%, 68%, 62% in adding 1.0 g/L. The higher enhancement of glutathione -S-transferase activity was observed in the ethanol extract as 257% compared to the control in adding 1.0 g/L. From the results, the biological activities of the crude extracts were equivalent or higher than glycyrrhizin and enzymatically hydrolyzed glycyrrhizin.

• Journal of Ginseng Research
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• v.17 no.1
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• pp.52-60
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• 1993

Panax ginseng has been extensively used in the traditional oriental medicine as a restorative, tonic and Prophylactic agent. Recently, several reports regarding to anticancer effects of Panax ginseng has accumulated. These studies emphasized the fact that the anticancer activities might be due to a glycoside group called ginsenoside or pan.u saponin which has a water soluble characteristic. However, the authors and collaborates demonstrated that a highly lipid soluble component in extract of Panax ginseng roots contains a considerable cytotoxic activities against marine leukemic cells (L1210, P388) and human censer cells (HRT-18, HT-29, HCT48). This study was devised to observe the cytotoxic activities of Petroleum-ether extract of Panax giuseng roots (crude GBD and its Partially Purified fraction from silicic acid column chromatography (7 : 3 GX) against sarcoma-180 (5-180) and Walker carcinosar- coma 256 (Walker 256) in vivo, and murine leukemic Lymphocytes (L1210) and human rectal cancer cells (HRT-18) and human colon cancer cells (HT-29 and HCT48) in vitro. Each cell-line was cultured in medium containing serial concentration of the crude GX or 7 : 3 GX in vitro. A highly lipid soluble compound in the extract of Panax ginseng root was cytocidal to murine leukemic cells and human colon and rectal cancer cells in vitro. In the meantime, ginseng saponin derivatives did not have cytotoxic effects at its corresponding concentration. The growth rates of the cancer cells in medium containing ginseng extracts were inhibited gradually to a significant degree roughly in proportion to the increase of the extract concentration. The cytotoxic activity of 7 : 3 GX was about 3 times more potent than that of crude GX, one unit of cytotoxic activity against L1210 cells being equivalent to 2.54 Ug and 058 Ug for the crude GX and 7 : 3 GX, respectively. The Ri value of the active compound on silica- gel thin layer chromatography with petroleum-ether/ethyl ether/acetic acid mixture (90 : 10 : 1, v/v/v) as a developing so lvent was 053. While, the Panaxydol and Panaxynol as active compounds were purified from Petroleum-ether extract of Panax ginseng root by Drs. Ahn and Kim, and author found out that the one unit of cytotoxic activity of the Panaxydol and Panaxynol against L1210 cells being equivalent to 056 Ug and 0.3918 respectively. The survival times of mice inoculated with S-180 cells were extended about 1.5 to 2 times by the 7 : 3 GX treatment compared with their control group. The significantly decreased hemoglobin values of rats after inoculation with Walker 256 were recovered to normal range by oral administration of the crude Gt The synthetic levels of protein, DNA and RNA in human colon and rectal cancer cells were significantly diminished by treatment with the crude GX, which can explain a part of the origin of its anticancer activity.

• Journal of environmental and Sanitary engineering
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• v.23 no.1
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• pp.17-23
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• 2008

Using the ethanol extract, Achyranthis Radix, various chemical characteristics were investigated. The nutritional compositions of the Achyranthis Radix extract were as follows;moisture 42.3%, crude protein 101.1%, crude fat 2.07%, ash 8.94%, and carbohydrate 36.5%. Among the free sugars, the maximum lactose concentration in the Achyranthis Radix extract was obtained at 0.0526 mg% and fructose, maltose, arabinose, and glucose were followed: 0.3654 mg%, 0.1160 mg%, 0.0365 mg% and 0.027 mg%, respectively. The total amino acid concentration of the Achyranthis Radix extract was 8908.3 mg% and concentrations of lysine, aspartic acid, glutamic acid, and arginine were 989.1 mg%, 954.4 mg%, 841.4 mg% and 763.2 mg%, respectively. Among various long chain fatty acids, the maximum concentrations of palmitic acid and linoleic acid were obtained at 47.8% and 31.058%, respectively. However, in the case of organic acid, only the oxalic acid and malic acid were determined. The potassium concentration in the Achyranthis Radix extract was relatively high and the concentrations of Ca, Mg, Fe, Na, Mn, and Zn were as follows: 275.3 mg%, 281.3 mg%, 119.4 mg%, 37.75 mg%, 10.43 mg% and 3.11 mg%, respectively. These results suggest that the Achyranthis Radix extract might have a possible positive effect for medical and edible purposes.

• Journal of Mushroom
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• v.11 no.3
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• pp.117-123
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• 2013

Hot water soluble extract was prepared from Hericium erinaceus and its neuritogenic activity on PC12h cells was analyzed, which is a clone originating from a rat pheochromocytomon. The moisture content of freeze dried hot water extract was 12.08%. The extract was mainly composed of carbohydrate (51.24%) followed by crude protein (24.04%), crude fat (0.26%), dietary fiber (5.09), and ash (12.18%). Fatty acids, glucan and inorganic constituents were found as minor components. The neuritogenic activity of hot water extract was evaluated under microscopic observation of neurite outgrowth in PC12h cells and by measuring the neurite length of induvidual cell. The extract exhibited strong effect of neurite outgrowth in a dose-dependent manner from 0.01 mg/mL to 1 mg/mL, in which longer neurite outgrowth was observed as the treatment dose increased.

• The Korean Journal of Physiology and Pharmacology
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• v.9 no.1
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• pp.55-62
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• 2005

Very little research has been carried out on safflower seed for the prevention and treatment of the bone deficiency diseases, including osteoporosis, which are supported by scientific evidences. In the present study, $3{\mu}l$ of 0.1% dried crude extract or $2{\mu}l$ of 0.1% dried aqueous fraction were shown to significantly accelerate the rate of differentiation of osteoblast. Also, the crude extract and aqueous fraction increased the $[Ca^{2+}]_i$ of the cultured osteoblast cells: $3{\mu}l$ of 0.1% dried crude extract and $2{\mu}l$ of 0.1% dried aqueous fraction significantly increased the $[Ca^{2+}]_i$ of the cultured osteoblast cells ($8{\times}10^{-4}$) to the extent that it deserves a considerable attention. Furthermore, the crude extract and aqueous fraction increased the $[Ca^{2+}]_i$ of the cultured osteoblast cells, and $300{\mu}M$ $Cd^{2+}$, specific calcium channel blocker, completely blocked the increase. Therefore, the increased $[Ca^{2+}]_i$ of the cultured osteoblast cells by safflower seed component continued to activate calcium channel.

• Korean Chemical Engineering Research
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• v.54 no.3
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• pp.374-379
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• 2016

When using an ionic liquid as co-solvent, the extraction efficiency of anticancer agent paclitaxel from biomass was dramatically improved. However, the residual ionic liquid had a significant negative effect on convenient and feasibility of following concentration and drying steps. In this study, a novel method was developed for the effective drying of the crude extract obtained from biomass extraction with ionic liquid. The residual ionic liquid was easily and conveniently removed by drying alone after pre-treatment and additional washing of a sample with water. The optimal crude extract/water ratio and mixing time for pre-treatment and crude extract/water ratio for additional washing were 1:70 (w/v), 4 min, and 1:100 (w/v), respectively. In the microwave-assisted drying process, the drying time was 9-fold shorter than in the vacuum oven drying process.

• Journal of Dairy Science and Biotechnology
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• v.35 no.2
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• pp.93-97
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• 2017

Linum usitatissimum L. (flaxseed) is emerging as an important functional food ingredient because of its rich contents, namely, ${\alpha}$-linolenic acid (ALA, omega-3 fatty acid), lignans, and fiber, which are potentially beneficial for human health. Furthermore, flax or flaxseed oil has also been incorporated as a functional food ingredient into various foods such as milk, dairy products, and meat products. Flaxseed is known to possess antimicrobial activity in vitro and in vivo, but its growth-stimulating effect on lactic acid bacteria is not clear. Hence, the objective of this study was to determine whether crude flaxseed extract stimulated the growth kefir-isolated lactic acid bacteria in vitro. The result of this study showed that Lactobacillus kefiranofaciens DN1, Lactobacillus brevis KCTC3102, Lactobacillus bulgaricus KCTC3635, and Lactobacillus plantarum KCTC3105 treated with $100{\mu}L$ of crude flaxseed extract showed significantly higher growth than the control treated with $100{\mu}L$ of water (p<0.05). Based on the results of this study, crude flaxseed extract could be used as a growth stimulator for lactic acid bacteria in various food applications, including production of milk and dairy products.

• Korean Journal of Medicinal Crop Science
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• v.13 no.6
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• pp.241-244
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• 2005

This study was carried out to compare growth characteristics, and extract and crude saponin contents of 4-year-old ginseng cultured by direct seeding and transplanting cultivation at several farms of the main producing district, Geumsan and Eumsung. Though root weight per plant of direct seeding cultivation was lower than that of transplanting cultivation, but yield of the former was higher than that of the latter owing to high rate of survival plant and Leaf Area Index. Dry matter partitioning ratio of direct seeding cultivation was high in primary root and low in secondary root because direct seeding cultivation elongated the length of primary root, while it suppressed the growth of secondary root. Ratio of rusty root was decreased in condition of direct seeding cultivation, while the contents of extract and crude saponin were lower than that of transplanting cultivation.