• Bulletin of the Korean Chemical Society
• /
• v.28 no.2
• /
• pp.246-250
• /
• 2007

The crude extract of kohlrabi (Brassica oleracea gongylodes) was prepared as a rich source of peroxidase and its enzymatic and specific activities in the presence of cross-linked polyvinylpyrrolidone as a stabilizer were determined. This extract was used to catalyze the oxidation of thiamine in the presence of hydrogen peroxide to thiochrome at pH 8.0. Thiochrome shows strong fluorescence at 436 nm with excitation at 370 nm. Based on the obtained results, a sensitive and simple spectrofluorimetric method was developed for the determination of thiamine. In the optimum conditions, the calibration graph was linear from 2 × 10-7 to 1 × 10-4 mol L-1, with a detection limit of 6.2 × 10-8 mol L-1. The relative standard deviation (RSD) was 1.2% for 5 × 10-6 mol L-1 thiamine. The method was successfully applied to the determination of thiamine in vitamin B1 and vitamin B complex tablets and vitamin B complex syrup.

• Ocean Systems Engineering
• /
• v.7 no.2
• /
• pp.107-120
• /
• 2017

Spud-can is used for fixing jack-up rig on seabed. It needs to be inserted up to the required depth during the installation process to secure enough soil reaction and prevent overturning accidents. On the other hand, it should be extracted from seabed soils as fast as possible during the extraction process to minimize the corresponding operational cost. To achieve such goals, spud-can may be equipped with water-jetting system including monitoring and control. To develop such a smart spud-can, a reliable numerical simulation tool is essential and it has also to be validated against physical model tests. In this regard, authors developed a numerical simulation tool by using a commercial program ANSYS with extended Drucker-Prager (EDP) formula. Authors also conducted small-scale (1/100) physical model tests for verification and calibration purpose. By using the numerical model, a systematic parametric study is conducted both for sand and K(kaolin)-clay with varying important soil parameters and the best estimated soil properties of the physical test are deduced. Then, by using the selected soil properties, the numerical and experimental results for a sand/K-clay multi-layer case are cross-checked to show reasonably good agreement. The validated numerical model will be useful in the next-stage study which includes controllable water-jetting.

• International Journal of Naval Architecture and Ocean Engineering
• /
• v.1 no.2
• /
• pp.64-69
• /
• 2009

The two-dimensional unsteady flow around a vertical axis turbine for tidal stream energy conversion was investigated using a computational fluid dynamics tool solving the Reynolds-Averaged Navier-Stokes equations. The geometry of the turbine blade section was NACA653-018 aiifoil. The computational analysis was done at several different angles of attack and the results were compared with the corresponding experimental data for validation and calibration. Simulations were then carried out for the two-dimensional cross section of a vertical axis turbine. The simulation results demonstrated the usefulness of the method for the typical unsteady flows around vertical axis turbines. The optimum turbine efficiency was achieved for carefully selected combinations of the number of blades and tip speed ratios.

• Bulletin of the Korean Space Science Society
• /
• 2010.04a
• /
• pp.28.2-28.2
• /
• 2010

영상레이더 위성으로부터 획득된 SAR 영상의 상대적/절대적 방사(radiometric) 정밀도를 만족시키기 위해서는 궤도상에서 검보정을 수행하여야 한다. 일반적으로 상대 방사 정밀도의 보정을 위해서는 아마존 일대와 같이 일정한 지표 반사도를 지니는 넓은 지역을 촬영함으로써, 지상에서 모델링된 안테나 패턴의 이상 유무를 검증한다. 절대 방사 정밀도를 결정하기 위해서는 보정계수(calibration constant)를 구해야 하는데, 이를 위해서 RCS(radar cross section) 값이 기 알려져 있는 지상의 CR(corner reflector)를 관측해야 한다. 대부분의 SAR 위성의 경우, 각 입사각별로 여러 개의 빔(beam)이 독립적으로 운용되고, 위성의 경로가 각 pass 사이 거의 일정한 간격을 가지기 때문에, 지상의 CR들에 대한 빔의 접근성이 상당히 제약을 많이 받게 된다. 즉, 개별 빔이 촬영할 수 있는 CR의 개수 및 동일 CR에 대한 촬영 빈번도가 많이 작을 수 있다는 것을 의미한다. 특히, CR이 빔폭의 중심에서 관측되어야 하는 요구사항이 추가로 반영될 경우 그 빈도수는 더욱더 영향을 받게된다. 이 연구에서는 고도 550 km, 28일의 지상반복주기(repeat ground track)를 가지는 여명 궤도(dawn-dusk orbit)를 가정하고, 각 빔별로 그 빔에 할당된 하나의 CR만을 촬영해야 된다는 조건하에, 빔 접근성의 요구사항을 최대로 만족시킬 수 있는 CR의 좌표들을 구하였다. 동시에, J2항만을 고려한 이상적 28일 지상반복궤도를 적용한 경우와 모든 중력섭동항을 적용한 최적궤도를 적용한 경우를 비교하여, 실질적 검보정 일정을 수립하였다.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2006.06a
• /
• pp.83-84
• /
• 2006

In this paper, we examine discharge characteristics of cross-linked polyethylene (since then; XLPE) according to thickness. Voltage was applied to power frequency by step method, and calibration of discharge was set to 50[pC] (slope=8.333). After the voltage was applied, for 10 [sec] (600 [cycle]), occurring discharge and number were detected. Determine of input pattern is difficult because discharge pattern is irregular. Therefore we investigated pattern using the K-means Analysis and Weibull function. Also we investigated variation of centroid and cluster.

• Geomechanics and Engineering
• /
• v.13 no.6
• /
• pp.1045-1055
• /
• 2017

Tensile strength is considered key properties for characterizing rock material in engineering project. It is determined by direct and indirect methods. Point load test is a useful testing method to estimate the tensile strengths of rocks. In this paper, the effects of rock shape on the point load index of gypsum are investigated by PFC2D simulation. For PFC simulating, initially calibration of PFC was performed with respect to the Brazilian experimental data to ensure the conformity of the simulated numerical models response. In second step, nineteen models with different shape were prepared and tested under point load test. According to the obtained results, as the size of the models increases, the point load strength index increases. It is also found that the shape of particles has no major effect on its tensile strength. Our findings show that the dominant failure pattern for numerical models is breaking the model into two pieces. Also a criterion was rendered numerically for determination of tensile strength of gypsum. The proposed criteria were cross checked with the results of experimental point load test.

• Journal of Preventive Veterinary Medicine
• /
• v.42 no.4
• /
• pp.171-176
• /
• 2018

A study of the tissue depletion of florfenicol (FFC) administered orally to pigs at a dose of 0.05 kg/ton feed for 7 days was performed. Sixteen healthy cross swine were administered with FFC. Four treated animals were arbitrarily selected to be sacrificed 1, 3 and 5 days after the end of treatment. FFC residue concentrations in muscle, liver, kidney, and fat were determined using high-performance liquid chromatography (HPLC) with ultraviolet photometric detector at 230 nm. The correlation coefficient ($R^2$) of the calibration curve for florfenicol amine (FFCa) was > 0.997 and the limits of detection and quantification were 0.012 and $0.040{\mu}g/mL$, respectively. Recovery rates in swine edible tissues ranged from 79.1 to 93.5%. In the FFC-treated group, FFC residues at 3 days post-treatment were below the maximum residue limits (MRLs) in muscle, kidney and fat, and those at 5 days post-administration were below the MRLs in all edible tissues. These results suggest that the withdrawal period of FFC after the drug treatment might be 5 days, which is a sufficient amount of time for reduction of the FFC residues below the MRLs in all edible tissues.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.23 no.10
• /
• pp.1204-1211
• /
• 2012

Measurement and analysis results of the extracted radiation-patterns from the field-experiments which were conducted to acquire the generic technology for calibration and validation of the satellite SAR system(Synthetic Aperture Radar) are presented in this study. Prototype of active transponder is adjustable within maximum 63.1 dBsm of RCS (Radar Cross Section) and includes the receiving-function with external receiver. To increase an accuracy of these field experiments, we repetitively measured satellite SAR systems of the same operating mode(i.e., COSMO-SkyMed No. 2 & 3, hh-pol., strip-map himage mode, 3 m resolution). Then, the reliability of experimental results was cross-checked through analysis of the RCS of active transponder on SAR image. The property of azimuth radiation patterns of satellite SAR system extracted from them has $0.352^{\circ}$ of HPBW(half-power beamwidth), $0.691^{\circ}$ of FNBW(first-null beamwidth), and 11.17 dB of PSLR(peak to side lobe ratio), respectively.

• Proceedings of the Ginseng society Conference
• /
• 2002.10a
• /
• pp.491-501
• /
• 2002

A cross-examination between KT&G Central Research Institute and Guangzhou Institute for Drug Control was carried out in order to select optimum conditions for extraction, separation and determination of ginsenosides in red ginseng and to propose a better method for the quantitative analysis of ginsenosides. The optimum extraction conditions of ginsenosides from red ginseng were as follows: the extraction solvent, $70\%$ methanol; the extraction temperature, $100^{\circ}C;$ the extraction time, 1 hour for once; and the repetition of extraction, twice. The optimum separation conditions of ginsenosides on the SepPak $C_{18}$ cartridge were as follows: the loaded amount, 0.4 g of methanol extract; the washing solvents, distilled water of 25 ml at first and then $30\%$ methanol of 25 ml; the elution solvent, $90\%$ methanol of 5 ml. The optimum HPLC conditions for the determination of ginsenosides were as follows: column, Lichrosorb $NH_2(25{\times}0.4cm,$ 5${\mu}m$, Merck Co.); mobile phase, a mixture of acetonitrile/water/isopropanol (80/5/15) and acetonitrile/water/isopropanol (80/20/15) with gradient system; and the detector, ELSD. On the basis of the optimum conditions a method for the quantitative analysis of ginsenosides were proposed and another cross-examination was carried out for the validation of the selected analytical method conditions. The coefficient of variances (CVs) on the contents of ginsenoside-$Rg_{1}$, -Re and $-Rb_1$ were lower than $3\%$ and the recovery rates of ginsenosides were $89.4\~95.7\%,$ which suggests that the above extraction and separation conditions may be reproducible and reasonable. For the selected HPLC/ELSD conditions, the CVs on the detector responses of ginsenoside-Rg, -Re and $-Rb_1$) were also lower than $3\%$, the regression coefficients for the calibration curves of ginsenosides were higher than 0.99 and two adjacent ginsenoside peaks were well separated, which suggests that the above HPLC/ELSD conditions may be good enough for the determination of ginsenosides.

• Journal of Biosystems Engineering
• /
• v.37 no.3
• /
• pp.177-183
• /
• 2012

Purpose: In near infrared spectroscopy, interactance configuration of a light source and a spectrometer probe can provide more information regarding fruit internal attributes, compared to reflectance and transmittance configuration. However, there is no through study on the parameters of interactance measurement setup. The objective of this study was to investigate the effect of the parameters on the estimation of soluble solids content (SSC) and firmness of muskmelons. Methods: Melon samples were taken from greenhouses at three different harvesting seasons. The prediction models were developed at three distances of 2, 5, and 8 cm between the light source and the spectrometer probe, three measurement points of 2, 3, and 6 evenly distributed on each sample, and different number of fruit samples for calibration models. The performance of the models was compared. Results: In the test at the three distances, the best results were found at a 5 cm distance. The coefficient of determination ($R_{cv}{^2}$) values of the cross-validation were 0.717 (standard error of prediction, SEP=$1.16^{\circ}Brix$) and 0.504 (SEP=4.31 N) for the estimation of SSC and firmness, respectively. The minimum measurement point required to fully represent the spectral characteristics of each fruit sample was 3. The highest $R_{cv}{^2}$ values were 0.736 (SEP=$0.87^{\circ}Brix$) and 0.644 (SEP=4.16 N) for the estimation of SSC and firmness, respectively. The performance of the models began to be saturated when 60 fruit samples were used for developing calibration models. The highest $R_{cv}{^2}$ of 0.713 (SEP=$0.88^{\circ}Brix$) and 0.750 (SEP=3.30 N) for the estimation of SSC and firmness, respectively, were achieved. Conclusions: The performance of the prediction models was quite different according to the condition of interactance measurement setup. In designing a fruit grading machine with interactance configuration, the parameters for interactance measurement setup should be chosen carefully.