• 한국자원식물학회지
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• 제33권5호
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• pp.536-549
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• 2020

콩은 전세계적으로 재배되는 중요한 작물 중에 하나로 최근, 표준유전체 해독과 함께 유전적, 표현형적으로 다양성을 가진 한국핵심집단이 구축됨에 따라 유전체 기반 분자 육종 연구, 유전자 교정 기술을 활용한 새로운 육종 소재 개발 연구가 가속화될 것으로 예상된다. 유전체 정보 기반 작물의 분자 육종 및 생명공학 연구를 통한 성공적인 작물의 개량을 위해서는 식물의 효율적인 조직배양 기술이 수반되어야 할 것이다. 그러나 반수체 생산, 원형질체 배양 및 형질전환 기술과 같은 콩의 조직배양 효율은 아직까지 높지 않고 일부 계통에 한정되어 이루어지고 있다. 본 논문에서는 콩의 분자육종 및 생명공학 기술의 적용을 위하여 다양한 콩 조직배양 기술에 관한 연구 동향을 분석하고 조직배양 효율에 영향을 미치는 요인들에 대한 정보를 제공하고자 하였다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 춘계학술대회
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• pp.20-20
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• 2022

• Journal of Plant Biotechnology
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• 제44권2호
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• pp.107-114
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• 2017

Implementation of crop improvement programs relies on genetic diversity. To overcome the limited occurrence of natural mutations, researchers and breeders applied diverse methods, ranging from conventional crossing to classical bio-technologies. Earlier generations of knockout and gain-of-function technologies often result in incomplete gene disruption or random insertions of transgenes into plant genomes. The newly developed editing tool, CRISPR/Cas9 system, not only provides a powerful platform to efficiently modify target traits, but also broadens the scope and prospects of genome editing. Customized Cas9/guide RNA (gRNA) systems suitable for efficient genomic modification of mammalian cells or plants have been reported. Following successful demonstration of this technology in mammalian cells, CRISPR/Cas9 was successfully adapted in plants, and accumulating evidence of its feasibility has been reported in model plants and major crops. Recently, a modified version of CRISPR/Cas9 with added novel functions has been developed that enables programmable direct irreversible conversion of a target DNA base. In this review, we summarized the milestone applications of CRISPR/Cas9 in plants with a focus on major crops. We also present the implications of an improved version of this technology in the current plant breeding programs.

• 한국농공학회논문집
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• 제62권2호
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• pp.1-15
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• 2020

In this study, the characteristics of crop damage area by flooding for 113 middle range watersheds during 2000-2016 were analyzed and future crop damage area by flooding were analyzed using 13 GCM outputs such as hourly maximum rainfall, 10-min maximum rainfall, number of days of 80 mm/day, daily rainfall maximum, annual rainfall amount associated with RCP 4.5 and RCP 8.5 scenarios and watershed characteristic data such as DEM, urbanization ratio, population density, asset density, road improvement ratio, river improvement ratio, drainage system improvement ratio, pumping capacity, detention basin capacity, and crop damage area by flooding. A constrained multiple linear regression model was used to construct the relationships between the crop damage area by flooding and other variables. Future flood index related to crop damage may mainly increase in the Mankyung watershed, Southwest part of Youngsan and Sumjin river basin and Southern part of Nackdong river basin. Results are useful to identify watersheds which need to establish strategies for responding to future flood damage.

• BMB Reports
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• 제40권2호
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• pp.247-260
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• 2007

Cinnamate 4-hydroxylase (C4H) is a key enzyme of phenylpropanoid pathway, which synthesizes numerous secondary metabolites to participate in development and adaption. Two C4H isoforms, the 2192-bp BnC4H-1 and 2108-bp BnC4H-2, were cloned from oilseed rape (Brassica napus). They both have two introns and a 1518-bp open reading frame encoding a 505-amino-acid polypeptide. BnC4H-1 is 57.73 kDa with an isoelectric point of 9.11, while 57.75 kDa and 9.13 for BnC4H-2. They share only 80.6% identities on nucleotide level but 96.6% identities and 98.4% positives on protein level. Showing highest homologies to Arabidopsis thaliana C4H, they possess a conserved p450 domain and all P450-featured motifs, and are identical to typical C4Hs at substrate-recognition sites and active site residues. They are most probably associated with endoplasmic reticulum by one or both of the N- and C-terminal transmembrane helices. Phosphorylation may be a necessary post-translational modification. Their secondary structures are dominated by alpha helices and random coils. Most helices locate in the central region, while extended strands mainly distribute before and after this region. Southern blot indicated about 9 or more C4H paralogs in B. napus. In hypocotyl, cotyledon, stem, flower, bud, young- and middle-stage seed, they are co-dominantly expressed. In root and old seed, BnC4H-2 is dominant over BnC4H-1, with a reverse trend in leaf and pericarp. Paralogous C4H numbers in Brassicaceae genomes and possible roles of conserved motifs in 5' UTR and the 2nd intron are discussed.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2006년도 Grop Science and Biotechnology in the 21st Century
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• pp.11-11
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• 2006

• 한국작물학회:학술대회논문집
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• 한국작물학회 1990년도 춘계 학술대회지
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• pp.92-93
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• 1990

• 한국작물학회:학술대회논문집
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• 한국작물학회 2019년도 춘계학술대회
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• pp.130-130
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• 2019

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.23-23
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• 2022

Recently, soybean production and market price are unstable, even if demand of soybean is maintained. Diverse conditions such as climate change, a decrease in rural population, and consuming affect food industry. In this situation, food security is soaring as important key-word again, and MAFRA is promoting policies for improving soybean self-sufficiency with the goal of 40% until 2030. The point of policy is to extend a production and stabilize a demand for soybean with supporting large-scale soybean paddy-field complex. According to the background, soybean breeding and production research in NICS are proceeded with three parts. First, production improvement with soybean cultivation land enlargement and high-yield cultivar development. Various growth period soybean cultivars for double cropping, irrigation management technologies in paddy field, and hyper-yield and specific-region adaptable cultivar development. Second, reduction of production expense with mechanized cultivation and digital-based field management technologies. Third, consumer-friendly and high quality soybeans with high protein cultivar for alternative protein usage and high food process-ability for soy milk, tofu, soybean sprouts, and grain usage. Each part need to be combined and advanced to improve soybean industry and soybean self-sufficiency.

• 한국작물학회지
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• 제59권2호
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• pp.109-127
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• 2014

Maize is one of the most important food and feed crops in the world including Southeast Asia. In spite of numberous efforts with conventional breeding, the maize productions remain low and the loss of yields by drought and downy mildew are still severe in Asia. Genetic improvement of maize has been performed with molecular marker and genetic engineering. Because maize is one of the most widely studied crop for its own genome and has tremendous diversity and variant, maize is considered as a forefront crop in development and estimation of molecular markers for agricultural useful trait in genetics and breeding. Using QTL (Quantitative Trait Loci) and MAS (Marker Assisted Breeding), molecular breeders are able to accelerate the development of drought tolerance or downy mildew resistance maize genotype. The present paper overviews QTL/MAS approaches towards improvement of maize production against drought and downy mildew. We also discuss here the trends and importance of molecular marker and mapping population in maize breeding.