• 제목/요약/키워드: Crop analysis

검색결과 3,071건 처리시간 0.025초

Separation and Characterization of Spikelet Proteins at Young Microspore Stage in Rice

  • Woo, Sun-Hee;Kim, Tae-Seon;Cho, Seong-Woo;Ahn, Jung-Gu;Chung, Keun-Yook;Lee, Byung-Moo;Cho, Yong-Gu;Kim, Hong-Sig;Song, Beom-Heon;Lee, Chul-Won;Jong, Seung-Keun
    • 한국작물학회지
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    • 제51권1호
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    • pp.66-72
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    • 2006
  • Spikelet proteins expressed at the young microspore stage in rice were separated and analysed by two-dimensional polyacrylamide gel electrophoresis (2DE). The separated proteins were electro blotted onto a polyvinylidene difluoride (PVDF) membrane, and 50 proteins were analyzed by a gas-phase protein sequencer. The N-terminal amino acid sequences of 20 out of 50 proteins were determined. N-terminal regions of the remaining proteins could not be sequenced because of blocking. The internal amino acid sequences of proteins were determined by sequence analysis of peptides obtained by the Cleveland peptide mapping method. Results revealed the presence of the photosynthetic apparatus at rice young microspore stage. Major proteins identified in this study could be used as a marker for various studies on physiological stresses.

Determination of Fatty Acid Composition in Peanut Seed by Near Infrared Reflectance Spectroscopy

  • Lee, Jeong Min;Pae, Suk-Bok;Choung, Myoung-Gun;Lee, Myoung-Hee;Kim, Sung-Up;Oh, Eun-young;Oh, Ki-Won;Jung, Chan-Sik;Oh, In Seok
    • 한국작물학회지
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    • 제61권1호
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    • pp.64-69
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    • 2016
  • This study was conducted to develop a fast and efficient screening method to determine the quantity of fatty acid in peanut oil for high oleate breeding program. A total of 329 peanut samples were used in this study, 227 of which were considered in the calibration equation development and 102 were utilized for validation, using near infrared reflectance spectroscopy (NIRS). The NIRS equations for all the seven fatty acids had low standard error of calibration (SEC) values, while high R2 values of 0.983 and 0.991 were obtained for oleic and linoleic acids, respectively in the calibration equation. Furthermore, the predicted means of the two main fatty acids in the calibration equation were very similar to the means based on gas chromatography (GC) analysis, ranging from 36.7 to 77.1% for oleic acid and 7.1 to 42.7% for linoleic acid. Based on the standard error of prediction (SEP), bias values, and $R^2$ statistics, the NIRS fatty acid equations were accurately predicted the concentrations of oleic and linoleic acids of the validation sample set. These results suggest that NIRS equations of oleic and linoleic acid can be used as a rapid mass screening method for fatty acid content analysis in peanut breeding program.

미질의 품종 및 환경변이 분석 II. 중산간지 밋 고냉지산미 품질 이화학적 특성 및 식미의 품종 및 산지간 변화 (Analysis of Varietal and Environmental Variation for Rice Quality Components II. Varietal and Environmental Variation in Commercial, Physicochemical, and Palatable Quality Components of Rice Produced in the Hilly and/or High Altitude Areas)

  • 최해춘;지정현;이종섭;조수연;박래경;김영배
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 1991년도 추계 학술대회지
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    • pp.54-55
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    • 1991
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미질의 품종 및 환경변이 분석 I. 중.남부평야지산미 품질, 이화학적 특성 및 식미의 품종 및 산지간 변이 (Analysis of Varietal and Environmental Variation for Rice Quality Components I. Varietal and Environmental Variation in Commercial, Physicochemical, and Palatable Quality Components of Rice Producted in the Middle and/or Southern Plain Areas)

  • 최해춘;지정현;이종섭;조수연;박래경;김영배
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 1991년도 추계 학술대회지
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    • pp.52-53
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    • 1991
  • PDF