• Title/Summary/Keyword: Critical experiment

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Screening of saline tolerant plants and development of biological monitoring technique for saline stress. II. Responses of emergence and early growth of several crop species to saline stress. (내염성 식물의 탐색 및 생물학적 염해 모니터링 기술의 개발 II.염분 스트레스에 대한 작물의 출현과 초기 생장 반응)

  • Shim, Sang-In;Lee, Sang-Gak;Kang, Byeung-Hoa
    • Korean Journal of Environmental Agriculture
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    • v.17 no.2
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    • pp.122-126
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    • 1998
  • This experiment was conducted to verify the responses of major crop species to saline stress.To determine the saline tolerance of crop species, sevral crop species were cultivated under sand-culture system using Hoagland's nutrient solution whit 200mM NaCl. The crop species showing saline tolerance were cotton(Gossypium indicum), maize(Zea mays), barely(Hordeum vulgare), and wheat(Triticum aestivum) but perilla (Perilla frutescens) and leguminous crops, mung bean(Phaseolus radiatus), azuki bean(Phaseolus angularis) and soy bean(Glycin max) showed very por tolerance. One the typical symptom was the darkening of leaf color due to increase of chlorophyll concentration.Among of the plant families, Fabaceae was the most susceptible but crop species belonging to Poaceae were more proper for cultivating on reclaimed tidal land in the course of desalinazation. It was suggrsted that the crop species belonging to Fabaceae, a sensitive family to soil salinity, must be cultivated when the soil salinity decreased below 10ds/m. To know the critical salinity level for crop growth,salinity of saline soil collected from reclaimed tidal land was adjusted from 10.0ds/m tp41.7ds/m with tap water. It was suggested that the ECs of the soil in which the plant height of each crop spicies was reduced to 50% of control plant were 22.6 and 21.7 ds/m in rice, barley, corn, mung bean, and soy bean,respectively.

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Screening of saline tolerant plants and development of biological monitoring technique for saline stress . 1. Survey of vegetation in saline region and determination of saline tolerance of the plant species of the region. (내염성 식물의 탐색 및 생물학적 염해 모니터링 기술의 개발 1. 염해지 식생분석 및 식물종의 내염성 평가)

  • Kang, Byeung-Hoa;Shim, Sang-In
    • Korean Journal of Environmental Agriculture
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    • v.17 no.1
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    • pp.26-33
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    • 1998
  • This experiment was conducted to classify the plant species occurring in the saline reclaimed land by saline tolerance. The vegetation of reclaimed land was composed of various plant species from halophyte to glycophyte showing different saline tolerances. In the investigated saline region, reclaimed land of Youngjong island, Inchun city, 175 species belonging to 32 families were found. Our survey was carried out in two region, having different salinity due to different desalinization. The electricalconductivity (EC) of more saline region showed 48.0mS/cm nd the other region showed 13.0mS/cm. It is assumed that intensity of precipitation and runoff of rainfall may cause salinity gradient in the investigated region. The plant species occurred in the experimental region were classified as 72 species of annual, 42 species of biennial, and 61 species of perennial according to life cycle. For knowing relationship between vegetation of saline region and saline tolerance of occurring species, we tested the saline susceptibility of plant species collected at the saline regions. Testing plants were cultured by nutrient solution containing 200 mM NaCl, the critical concentration of survival in glycophytes. The saline tolerance was graded by the growing capacity in the sand-culture system. The more saline-tolerant species screened by sand culture were Atriplex gmelini, Suaeda asparagoides, Aster tripolium, Suaeda maritima, Salicornia herbacea, and Suaeda japonica. The most saline tolerant family was Chenopodiaceae. Poaceae, Cyperaceae, and Brassicaceae showed relatively high tolerance to saline stress. In the course of growth under the high saline condition, the most noticeable change was the darkening of leaves by increasing of chlorophyll content. The chlorophyll contents were increased with saline stress in most species.

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Designing mobile personal assistant agent based on users' experience and their position information (위치정보 및 사용자 경험을 반영하는 모바일 PA에이전트의 설계)

  • Kang, Shin-Bong;Noh, Sang-Uk
    • Journal of Internet Computing and Services
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    • v.12 no.1
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    • pp.99-110
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    • 2011
  • Mobile environments rapidly changing and digital convergence widely employed, mobile devices including smart phones have been playing a critical role that changes users' lifestyle in the areas of entertainments, businesses and information services. The various services using mobile devices are developing to meet the personal needs of users in the mobile environments. Especially, an LBS (Location-Based Service) is combined with other services and contents such as augmented reality, mobile SNS (Social Network Service), games, and searching, which can provide convenient and useful services to mobile users. In this paper, we design and implement the prototype of mobile personal assistant (PA) agents. Our personal assistant agent helps users do some tasks by hiding the complexity of difficult tasks, performing tasks on behalf of the users, and reflecting the preferences of users. To identify user's preferences and provide personalized services, clustering and classification algorithms of data mining are applied. The clusters of the log data using clustering algorithms are made by measuring the dissimilarity between two objects based on usage patterns. The classification algorithms produce user profiles within each cluster, which make it possible for PA agents to provide users with personalized services and contents. In the experiment, we measured the classification accuracy of user model clustered using clustering algorithms. It turned out that the classification accuracy using our method was increased by 17.42%, compared with that using other clustering algorithms.

Evaluation on the Behavior of Slip Critical Joints with TS High Strength Bolts Subjected to a Size of Bolt Holes (볼트 구멍 크기에 따른 TS 고력볼트 접합부 거동 평가)

  • Lee, Hyeon Ju;Kim, Kang Seok;Nah, Hwan Seon;Lee, Kang Min;Kim, Hyun Chul
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.15 no.2
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    • pp.136-143
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    • 2011
  • The oversized and slotted holes are frequently required for the built-up in construction sites. The foreign provisions specify the reduction of the slip load subjected to the size of bolt holes and the direction of load. There are no domestic building codes and researches on the bolt holes. Therefore, it is necessary to evaluate a change of joint strength quantitatively according to the bolt-hole size and surface condition by means of experiment. This study was conducted to evaluate the slip load subjected to the size of bolt holes, and measured on a change of clamping force of high strength bolts during 168 and 800 hours to analyze the trend of relaxation after fastening bolts. Torque shear bolts defined on KS B 2819 was used for the specimen. Test results exhibit that the variation on the slip load of the others was below 10% by contrast with the standard hole and the highest rate of relaxation was 2.66% of the initial clamping force at the case of the long-slotted hole of 2.5D.

An Action-Generation Method of Virtual Characters using Programming by Demonstration (Programming by Demonstration을 이용한 가상 캐릭터의 행동 생성 기법)

  • Sung, Yun-Sick;Cho, Kyung-Eun;Um, Ky-Hyun
    • Journal of Korea Game Society
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    • v.11 no.2
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    • pp.141-149
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    • 2011
  • The substantial effort is required to make a virtual character smoothly move like a human being in the virtual environment. Since a virtual character acts on the basis of the actions, it is the most critical to define actions for smooth flow of action. It has been actively studied the programming by demonstration which defines series of actions to be performed by a virtual character based on the actions operated by a person. However, such approaches can't easily draw many sequential actions because they create sequential actions in the same length all the time or restrict the actions used to create such actions. For smooth flow of action, it is required to derive sequential actions as much as possible from the actions of a virtual character and to select the representative set of actions. Accordingly, it is necessary to study how to create sequential actions as reducing diverse limits. This study suggests the approach to select sequential actions suitable for a virtual character by collecting the actions of a character manipulated by a person and deriving a set of actions to be performed by a virtual character. The experiment describes the process to create the actions by applying the approach suggested in this study to the driving game. In accordance with the analysis results, it was found that a set of actions performed by a person was generated without being restricted by a length or a part to divide. Finally, we confirmed that the suggested method selects the best sequential actions, appropriate to virtual character, among more generated actions.

Optimization of monitoring methods for air-borne bacteria in the environmental conditions of pig facilities (무균 돈사 환경 모니터링을 위한 대기 중 미생물 탐지기법 확립)

  • Lee, Deok-Yong;Seo, Yeon-Soo;Kang, Sang-Gyun;Yoo, Han Sang
    • Korean Journal of Veterinary Research
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    • v.46 no.3
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    • pp.255-261
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    • 2006
  • Experimental animals have been used to biological and medical purposes and the animals must be, for these purposes, healthy and clean to microbial infection. However, the animals can be easily exposed to pathogenic microorganism via several routes. Of the routes, environmental conditions are the most important factors to keep the animals healthy and clean, especially air condition. Monitoring of air-condition has been required to keep the animal healthy and clean. However, any guideline is not available for experimental conditions with pigs. Therefore, the sampling times and points were compared in different conditions to establish an optimal protocol for monitoring of air borne bacteria. Tryptic soy agar(TSA), blood agar containing 5% defibrinated sheep blood and Sabraud dextrose agar(SDA) were used as media to capture total bacteria, pathogenic bacteria and fungi, respectively. Two methods, compulsive capture using an air-sampler and capturing fall-down bacteria were used to capture the microorganisms in the air. The points and time of capturing were different at each experiment. Air borne microorganisms were captured at three and five points in the open and closed equipments, respectively. Air was collected using an air-sampler for 1 min and 5 min and the agar plates as open status were left from 30 min to 2hr. At first, we monitored an experimental laboratory which dealt with several pathogenic bacteria and then, a protocol obtained from the investigation was applied to open or close experimental conditions with pigs. Number of bacteria was high from 10:00 to 15:00, especially on 13:30-15:30 but sharply decreased after 17:00. The tendency of the number of bacteria was similar between two methods even though the absolute number was higher with air sampler. Critical difference in the number of cells was observed at 5 min with air sampler and 2 hr with fall-down capturing method. However, 1 min with air sampler and 1 hr with fall-down capturing were the best condition to identify bacterial species collected from the air. Number of bacteria were different depending on the sampling points in closed condition but not in opened condition. Based on our results, a guide-line was suggested for screening air-borne microorganism in the experimental conditions with pigs.

Analysis of Nitrosation Inhibition and Antioxidant Effect by Phyto-Extract Mixture (식물추출 혼합제재인 phyto-extract mixture의 니트로세이션 억제능과 항산화능 분석)

  • Kim, Ji-Hoon;Shin, Mi-Jung;Cho, Hee-Jae;Lee, Sang-Won;Jeong, Jong-Moon
    • Korean Journal of Food Science and Technology
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    • v.33 no.6
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    • pp.656-663
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    • 2001
  • The most representative nitrosamine derived from nicotine, nitrosamine-4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone(NNK), has been reported to cause lung cancer in A/J mice. It has been also demonstrated that NNK-induced lung tumorigenesis involves $O^6-methylguanine(O^6MeG)$ formation, leading to $GC\;{\rightarrow}\;AT$ transitional mispairing during DNA replication. Our in vitro experiment, modified from the method of DBA assay, examined the ability of phyto-extract mixture to inhibit the metabolism of nicotine to nitrosamines. The production of nitromorpholine from morpholine was inhibited about 75% at the concentration of 20 mg/mL of phyto-extract mixture, which was lower than vitamine C and green tea powder. NNK, which is a pro-carcinogen in laboratory animals, is hydroxylated primarily in liver and lung by CYP 1A2, 2A6 and 3A4. A critical phase. of NNK activation is its change to an unstable metabolite methyl-diazohydroxide via CYP-mediated ${\alpha}-hydroxylation$; and then it provides a methyl group to the DNA to form DNA adducts which can easily induce mutations. $Aroclor^R$ 1254 was used to induce CYPs in the liver of a Sprague-Dawley rat. The ability of various test samples to inhibit CYPs that participate in NNK activation was evaluated, following the removal of the liver from the rat. Microsomal CYPlA2 catalyzing the conversion of NNK into strong carcinogenic chemicals was inhibited more efficiently by phyto-extract mixture than green tea powder. These results indicate that phyto-extract mixture can be used to reduce $O^6MeG$ DNA adducts for chemoprevention.

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Measurement of Plasma Parameters (Te and Ne) and Reactive Oxygen Species in Nonthermal Bioplasma Operating at Atmospheric Pressure

  • Choi, Eun Ha;Kim, Yong Hee;Kwon, Gi Chung;Choi, Jin Joo;Cho, Guang Sup;Uhm, Han Sup;Kim, Doyoung;Han, Yong Gyu;Suanpoot, Pradoong
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.141-141
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    • 2013
  • We have generated the needle-typed nonthermal plasma jet by using an Ar gas flow at atmospheric pressure. Diagnostics of electron temperature anddensity is critical factors in optimization of the atmospheric plasma jet source in accordance with the gas flow rate. We have investigated the electron temperature and density of plasma jet by selecting the four metastable Ar emission lines based on the atmospheric collisional radiative model and radial profile characteristics of current density, respectively. The averaged electron temperature and electron density for this plasma jet are found to be ~1.6 eV and ~$3.2{\times}10^{12}cm^{-3}$, respectively, in this experiment. The densities of OH radical species inside the various bio-solutions are found to be higher by about 4~9 times than those on the surface when the argon bioplasma jet has been bombarded onto the bio-solution surface. The densities of the OH radicalspecies inside the DI water, DMEM, and PBS are measured to be about $4.3{\times}10^{16}cm^{-3}$, $2.2{\times}10^{16}cm^{-3}$, and $2.1{\times}10^{16}cm^{-3}$, respectively, at 2 mm downstream from the surface under optimized Ar gas flow 250 sccm.

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Permeation Properties of Single Gases ($N_2$, $O_2$, $SF_6$, $CF_4$) through PDMS and PEBAX Membranes (PDMS와 PEBAX 분리막을 통한 단일기체($N_2$, $O_2$, $SF_6$, $CF_4$) 투과 특성)

  • Kim, Hanbyul;Lee, Minwoo;Park, Wankeun;Lee, Soonjae;Lee, Hyunkyung;Lee, Sanghyup
    • Membrane Journal
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    • v.22 no.3
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    • pp.201-207
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    • 2012
  • In this study, we investigated permeation of single gas ($N_2$, $O_2$, $CF_4$, and $SF_6$) through flat sheet membrane composed of PDMS (poly-dimethylsiloxane) and PEBAX (polyether block amides). Gas permeation experiment was performed with various feed pressure. Permeability was estimated using permeation flux measured by continuous-flow technique. The permeability of gases except $SF_6$ in PDMS were decreased with the upstream pressure increased. $SF_6$ is much more permeable than $CF_4$, which is due to higher critical temperature of $SF_6$. The permeability decreased in the following order: $O_2$ > $N_2$ > $SF_6$ > $CF_4$. On the other hand, the permeability of gases in PEBAX followed the order: $O_2$ > $N_2$ > $CF_4$ > $SF_6$ which are opposite of the order of kinematic diameter (${\AA}$)($SF_6$ > $CF_4$ > $N_2$ > $O_2$). The $SF_6/CF_4$ pure gas selectivity in PDMS was 2.1 at 0.7 MPa.

Cultural and Physiological Conditions for T-2 Toxin Production by Fusarium sp. (Fusarium 균주의 배양 조건 및 생리적 조건에 따른 T-2 toxin의 생성 조건)

  • 홍성희;양규환
    • Korean Journal of Microbiology
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    • v.36 no.2
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    • pp.91-96
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    • 2000
  • The cultural and physiological conditions for the T-2 toxin [4,15-diacetoxy-8-(3-mety1butyloxy)-12,13- epoxy-trichothec-9-en-3-01, $C_{24}H_{30}O_9$] production by Fusarium spp. were studied. Thin layer chromatography (TLC) assay and the microbiological assay uslng Rhodotomla rubra were used to quantitate tbe T- 2 toxin. Among the four strains of Fusarium spp., F tn'cinctum NRRL 3299 was best for T-2 toxin production. In solid culture, white com grit medium was best for T-2 toxm production. Temperature played a critical role in the production of T-2 toxin. T-2 toxin production was favored by long duration of low-temperature incubation. The growth and toxin production were relatively high on galactose, fructose, glucose, and sucrose media, when each was used as a sole carbon source, and relatively low on sorbitol, glycerol, and lactose media. For nitrogen sources, $NH_4^(+) and NO_3^{-}were used well as a sole nitrogen source, but $NO_2^-$ was not used. Initial pH and speed of shaker also affected the production of T-2 toxin. From temperature shifting experiment, it is clear that T-2 toxin metabolic pathway is regulated by temperature-dependent enzyme depression or enzyme induction system.

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