• Journal of Microbiology and Biotechnology
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• v.31 no.11
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• pp.1583-1590
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• 2021

Studies have demonstrated that PE_PGRS45 is constitutively expressed under various environmental conditions (such as nutrient depletion, hypoxia, and low pH) of the in vitro growth conditions examined, indicating that PE_PGRS45 protein is critical to the basic functions of Mycobacterium tuberculosis. However, there are few reports about the biochemical function and pathogenic mechanism of PE_PGRS45 protein. The fact that this M. tuberculosis gene is not easily expressed in E. coli may be mainly due to the high content of G+C and the use of unique codons. Fusion tags are indispensable tools used to improve the soluble expression of recombinant proteins and accelerate the characterization of protein structure and function. In the present study, His6, Trx, and His6-MBP were used as fusion tags, but only MBP-PE_PGRS45 was expressed solubly. The purification using His6-MBP tag-specific binding to the Ni column was easy to separate after the tag cleavage. We used the purified PE_PGRS45 to immunize New Zealand rabbits and obtained anti-PE_PGRS45 serum. We found that the titer of polyclonal antibodies against PE_PGR45 was higher than 1:256000. The result shows that purified PE_PGRS45 can induce New Zealand rabbits to produce high-titer antibodies. In conclusion, the recombinant protein PE_PGRS45 was successfully expressed in E. coli and specific antiserum was prepared, which will be followed by further evaluation of these specific antigens to develop highly sensitive and specific diagnostic tests for tuberculosis.

• Journal of the Microelectronics and Packaging Society
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• v.29 no.3
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• pp.37-42
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• 2022

Recently, the semiconductor package structures are becoming thinner and more complex. As the thickness decrease, interfacial delamination due to material mismatch can be further maximized, so the reliability of interface is a critical issue in industry field. Especially, the polymers, which are widely used in semiconductor packaging, are significantly affected by the temperature and moisture. Therefore, in this study, the delamination prediction at the interface of package structure was performed through finite element analysis considering the moisture absorption and desorption under the various temperature conditions. The material properties such as diffusivity and saturated moisture content were obtained from moisture absorption test. The hygro-swelling coefficients of each material were analyzed through TMA and TGA after the moisture absorption. The micro-shear test was conducted to evaluate the adhesion strength of each interface at various temperatures considering the moisture effect. The finite element analysis of interfacial delamination was performed that considers both deformation due to temperature and moisture absorption. Consequently, the interfacial delamination was successfully predicted in consideration of the in-situ moisture desorption and temperature behavior during the reflow process.

• Korean Journal of Poultry Science
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• v.48 no.4
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• pp.177-184
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• 2021

Sex identification in day-old chicks is essential in the poultry industry. Currently, the feather-sexing using a sex-linked inheritance is a chick sexing method used extensively in the poultry industry. For chicks produced by feather-sexing, all females are early-feathering (EF) chicks and all males are late-feathering (LF) chicks. Therefore, investigating the effects of EF and LF phenotypes on production traits of chickens is critical. The purpose of this study was to analyze and compare the production performance between EF and LF chickens in Korean native commercial chickens. The results showed that the survival rate of the EF chickens was significantly higher than that of the LF chickens, from hatching to 12 weeks of age (P<0.05), with the highest difference observed in females. However, no significant difference was observed in body weight between the EF and LF chickens at almost all ages. In addition, no significant difference was observed between in feed utility, such as average daily feed intake and feed conversion ratio, between the EF and LF groups. In conclusion, although the survival rate of early-feathering chickens was superior to that of late-feathering chickens, no significant difference was observed in growth performance and feed utility among Korean native chickens. The results suggest that production capacity is not influenced by feathering type in the establishment of a breeder structure and production system for feather-sexing Korean native chickens, which is valuable for the practical application of feather-sexing in the poultry industry.

• Journal of the Korean Geotechnical Society
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• v.38 no.12
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• pp.45-65
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• 2022

In this paper, a diaphragm wall that supports soils and rock was modeled using FLAC, a finite difference analysis program, to evaluate the seismic behavior of temporary retaining structures in a deep excavation. The appropriateness of the numerical model was verified by comparing its results with those of the centrifuge test performed in a similar condition. The bending moment distribution along the diaphragm wall shows a very similar tendency, and the maximum acceleration obtained at the backfill and top of the wall shows a difference within 5%. Based on the developed model, a parametric study was conducted in various input earthquake, ground, and excavation conditions. The maximum structural forces and bending moment under earthquake loading were compared with the maximum values during excavation, from which the critical condition that requires a seismic design was roughly sorted out. The maximum bending moment of a wall that retains soil layers increased 17%. Particularly, the axial force of struts located in loose soils increased 32% under 100 years return period of an earthquake event, which strongly is estimated to require seismic design for structural safety.

• 지역과문화
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• v.7 no.3
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• pp.81-108
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• 2020

This study aims to critically consider the local cultural policies of the government and local governments by village mural. Village murals, which began to be painted in the late 1990s, have been a nationwide cultural phenomenon for decades. In particular, it was consistently used as an important element of various local cultural policies implemented by the government and local governments. The government and local governments have been working on the project for two purposes: 'improving the residential environment' and 'activating tourism' but it has largely focused on 'activating tourism'. This study grasped the concrete status of major village murals across the country through field surveys, one of the case study methods. Also, through the analysis of official documents, the government and local governments operating in the village mural project were analyzed for the policy basis and administrative management plan. As a result of on-site surveys, most of the village murals across the country had problems such as uniform subject matter, low level representation, and poor follow-up management. This is partly based on the experiences and abilities of organizations and artists who participated in the village mural project, but fundamentally, it is due to the projects of the government and local governments that focused on short-term visual tourism rather than long-term improvement of the residential environment. It was confirmed that the projects of the government and local governments were carried out through vertical integration and that there were problems in the actual work, such as requiring specific topics, subject matter, designs and colors. As a result, the village murals 'objectify' the residents and 'romanticized' the place, resulting in a unified visual representation. Therefore, this study suggests that the administration and accounting should be strictly managed, but in actual work, the vertical integration structure should be eased by simplifying various documents and procedures, as well as rich cultural understanding of the target place, selection of various topics, and original subject matter and expressions.

• The Journal of the Convergence on Culture Technology
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• v.8 no.5
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• pp.171-176
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• 2022

This paper examines the historical meaning and value of sound art curating as a key type of interdisciplinary and convergence art practice that has been unfolding since the mid-20th century. Accordingly, this paper summarizes the developmental process from the beginning of 'sound art' to the present, but examines the context of visual art in which the material 'sound' functioned in chronological order, and focuses on curating cases of major sound art exhibitions. The purpose of this study is to analyze the impact and contemporary significance of the provided aesthetic experience. To this end, the text is divided into three sections and developed. The first section recognizes that the late 19th century futurist and Dadaist sound poetry, followed by Marcel Duchamp's 1913 attempt to combine musical score with visual art, had a profound influence on the visual music of avant-garde composer John Cage. This explains why this background caused the emergence of exhibitions dealing with 'sound' as a new medium. The second section explains that in the 1970s, sound as an artistic medium played a role in reflecting the critical relationship with the exhibition space dominated by visuality. In the third section, we analyze the curatorial methodology that allows the audience to experience sound as if it were a visual object within the organization of the exhibition hall from the 1980s to the present. Through this process, this paper critically treats the historical practice of customizing the perceptual structure in the exhibition hall, and considers the meaningful methodology of sound art curating considering the role of sound full of vitality in the contemporary art scene.

• Journal of the Daesoon Academy of Sciences
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• v.28
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• pp.243-265
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• 2017

In this research, I define 'conscience' in Daesoon thought as the 'Restorative capability of returning to one's roots.' The notion of conscience forms a structure of separation and return, and it is connected to the ascetic aim of realizing human perfection. The conscience opens up potential possibilities and leads realization of potential possibilities by returning to the the point of origin. In the middle of separation and return, the conscience acts as the power of subjectivity possessed by human beings which is known as 'In jon (Human Nobility)' in Daesoon thought. The concept of conscience in Daesoon thought is connected with the subjectivity of In jon and free will as well as the character of subjectivity. This shares commonalities with critical thinking, modern characteristics, and the subjectivity of Heidegger's existentialism. Heidegger describes human fate from an existentialist vantage point using terms such as dasein, Geworfenheit, and Entwurf, and establishes human existence as an act of self-recovery from within in a lonely existential establishment. Daesoon thought implies that humanity is the root of ultimate reality, and this description is in sharp contrast with the thrownness (Geworfenheit) of Heidegger's subjectivity. Therefore, Daesoon thought can be seen as unique in its characterization of humanity as being connected to the root of ultimate reality, autonomy, and independent existence.

• Journal of the Korean Geotechnical Society
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• v.23 no.2
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• pp.47-60
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• 2007

Conventional active surface wave measurements performed using a transient or continuous source are often limited in the maximum depth of penetration due to the difficulty of generating low-frequency energy with reasonably portable sources. This limitation may inhibit accurate seismic site response calculations because of the inability to define deeper subsurface structure. By measuring surface wave generated by passive sources including microtremors and cultural noise, it is possible to overcome this problem and develop soil stiffness profiles to much larger depth. Reliability of dispersion estimates from the passive surface wave measurements is critical to present reliable shear wave velocity profiles and can be improved by the measurements and analyses of passive surface waves based on correct understanding of systematic errors included in passive dispersion data. In this study, the systematic errors caused by poor wavenumber resolution and energy leakage into sidelobes in passive tests are mainly explored. Recommendations for reliable passive surface wave measurements and dispersion estimates are presented and illustrated at a site in San Jose, California, U.S.

• Applied Chemistry for Engineering
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• v.34 no.3
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• pp.264-271
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• 2023

In this study, ASCO^Ⓡ SLES-430 surfactant was synthesized by adducting 3 moles of ethylene oxide and 1 mole of propylene oxide to lauryl alcohol followed by a sulfation process, and the structure of the synthesized ASCO^Ⓡ SLES-430 was elucidated by performing FT-IR, ¹H-NMR and ¹³C-NMR analyses. Interfacial properties such as critical micelle concentration, static surface tension, emulsification index, and contact angle were measured, and environmental compatibility indices such as oral toxicity and skin irritation were also estimated for ASCO^Ⓡ SLES-430. Both results were compared with ASCO^Ⓡ SLES-226 and ASCO^Ⓡ SLES-328 SLES surfactants possessing 2 moles and 3 moles of ethylene oxide, respectively. In particular, both foaming ability and foam stability were evaluated for ASCO^Ⓡ SLES-430 and compared with ASCO^Ⓡ SLES-226 and ASCO^Ⓡ SLES-328, which have been widely used in detergent products, in order to test the potential applicability of ASCO^Ⓡ SLES-430 in detergent product formulation for a small capacity built-in washing machine.

• Analyses & Alternatives
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• v.3 no.1
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• pp.93-133
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• 2019

Throughout history, there are three clues of dualistic structures for the development of Hong Kong's nativism. First, inward attribution and outward lookingare two paths to the formation of Hong Kong's nativism. In the dualistic framework of "self-others", nativism is formed. The formation path of "outward looking"can be seen everywhere in the construction history of Hong Kong's nativism. It is under the reflection of "two mirrors" with Britain and Chinese mainland that Hong Kong people acquire the concept of "Hong Kong's nativism". Second, there are two aspects of Hong Kong's nativism: economic and cultural aspect and political aspect. With the gradual development of Hong Kong's history, these two aspects come into being and are closely bound up, thus constituting Hong Kong's nativism today. The third clue is the most critical one. The subjectivity of colonization and decolonization are two different forms of Hong Kong people's subjectivity. These three clues run through the whole process of the construction of Hong Kong nativism, and are carried out in three stages of development: "Origin (1960s-1970s): Preliminary Construction of Hong Kong's Nativism", "Development of Hong Kong's Nativism (1980s-1997): Awakening of Political Aspect" and "Formation and Alienation of Hong Kong's Nativism (1997-present): Deformed "decolonization". Along the evolution of Hong Kong's nativism, with the disintegration of colonialism, Hong Kong people have gradually transited from the subjectivity of colonization to the subjectivity of decolonization, but the process of "decolonization" has not been completed up to now.When nativism loses its native complex from the perspective of "inherent in China", and further develops into the "separatism" of anti-constitutional system and anti-national continuity and unity, it will challenge the stability of the relationship between the central government and the Special Administrative Region under the "one country, two systems". At the same time, it will have a greater impact on the political structure and the rule of law system of Hong Kong, and trigger a series of public law problems that need to be solved urgently. In this regard, on the one hand, we should re-clarify the relationship between the central government and the region under the "one country, two systems" in light of the new situation of democratic political development in Hong Kong, and improve Hong Kong's governance mechanism on the basis of the constitution and the basic law; on the other hand, we should actively learn from the German defensive democracy system to systematically interpret, integrate and apply Hong Kong's existing legal resources so as to effectively curb the development of local separatist forces.