• Journal of the Korean Applied Science and Technology
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• v.17 no.4
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• pp.213-225
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• 2000

Sophorolipids were biosynthesized using a strain of yeast, Torulopsis bombicola ATCC22214. It has been reported that this yeast gives the highest yields for the production of biosurfactant sophorolipids. Hence, this yeast was used in this study. One of the objectives of this study is to increase the yield of the sophorolipid synthesis. To meet this end, basic culture medium was formulated on the basis of literature research to-date. When this medium was used, the increase in yield from 15% to 150% was observed compared to using the media in the literature. To examine how the interfacial characteristics of sophorolipids change with substrate, glucose (the first carbon source) was maintained in the media and after being cultured for three days, the second carbon sources such as alkanes, vegetable oils, alcohols or organic acids were added. The whole broth was extracted twice with ethyl acetate and the extract was analyzed by thin layer chromatograhy(TLC). After qualitative analyses by TLC, surface tensions of sophorolipids were measured by the Wilhelmy plate method and critical micelle concentration(CMC) was determined using these surface tension data. Also, interfacial tensions were measured by the spinning drop method and emulsions of the three-component water/decane/sophorolipid system were tested. Sophorolipids were effective and efficient in terms of surface tension reduction and CMC, but they were ineffective as emulsifiers because emulsions were separated within 30 minutes.

• Journal of Microbiology and Biotechnology
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• v.30 no.5
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• pp.753-761
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• 2020

To determine the role of pyruvate dehydrogenase complex (PDHC) in Klebsiella pneumoniae, the growth and metabolism of PDHC-deficient mutant in glycerol-based medium were analyzed and compared with those of other strains. Under aerobic conditions, the PDHC activity was fourfold higher than that of pyruvate formate lyase (PFL), and blocking of PDHC caused severe growth defect and pyruvate accumulation, indicating that the carbon flux through pyruvate to acetyl coenzyme A mainly depended on PDHC. Under anaerobic conditions, although the PDHC activity was only 50% of that of PFL, blocking of PDHC resulted in more growth defect than blocking of PFL. Subsequently, combined with the requirement of CO₂ and intracellular redox status, it was presumed that the critical role of PDHC was to provide NADH for the anaerobic growth of K. pneumoniae. This presumption was confirmed in the PDHC-deficient mutant by further blocking one of the formate dehydrogenases, FdnGHI. Besides, based on our data, it can also be suggested that an improvement in the carbon flux in the PFL-deficient mutant could be an effective strategy to construct high-yielding 1,3-propanediol-producing K. pneumoniae strain.

• Journal of Biosystems Engineering
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• v.41 no.3
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• pp.153-160
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• 2016

Purpose: This study was conducted to experimentally investigate the structural safety of and identify critical locations in a front-end loader under impact loads. Methods: Impact and static tests were conducted on a commonly used front-end loader mounted on a tractor. In the impact test, the bucket of the front-end loader with maximum live load was raised to its maximum lift height and was allowed to free fall to a height of 500 mm above the ground where it was stopped abruptly. For the static test, the bucket with maximum live load was raised and held at the maximum lift height, median height, and a height of 500 mm from the ground. Strain gages were attached at twenty-three main locations on the front-end loader, and the maximum stresses and strains were measured during respective impact and static tests. Results: Stresses and strains at the same location on the loader were higher in the impact test than in the static test, for most of measurement locations. This indicated that the front-end loader was put under a severe environment during impact loading. The safety factors for stresses were higher than 1.0 at all locations during impact and static tests. Conclusions: Since the lowest safety factor was higher than 1.0, the front-end loader was considered as structurally safe under impact loads. However, caution must be exercised at the locations having relatively low safety factors because failure may occur at these locations under high impact loads. These important design locations were identified to be the bucket link elements and the connection elements between the tractor frame and front-end loader. A robust design is required for these elements because of their high failure probability caused by excessive impact stress.

• Journal of Welding and Joining
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• v.17 no.1
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• pp.124-132
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• 1999

Thermal fatigue strength of the solder joints is the most critical issue for TSOP(Thin Small Outline Package) because the leads of this package are extremely short and thermal deformation cannot be absorbed by the deflection of the lead. And the TSOP body can be subject to early fatigue failures in thermal cycle environments. This paper was discussed distribution of thermal stresses at near the joint between silicon chip and die pad and investigated their reliability of solder joints of TSOP with 42 alloy clad lead frame on printed circuit board through FEM and 3 different thermal cycling tests. It has been found that the stress concentration around the encapsulated edge structure for internal crack between the silicon chip and Cu alloy die pad. And using 42 alloy clad, The reliability of TSOP body was improved. In case of using 42 alloy clad die pad(t=0.03mm). $$\sigma$_{VMmax}$ is 69Mpa. It is showed that 15% improvement of the strength in the TSOP body in comparison with using Cu alloy die pad $($\sigma$_{VMmax}$=81MPa). In solder joint of TSOP, the maximum equivalent plastic strain and Von Mises stress concentrate on the heel of solder fillet and crack was initiated in it's region and propagated through the interface between lead and solder. Finally, the modified Manson-Coffin equation and relationship of the ratio of $N_{f}$ to nest(η) and cumulative fracture probability(f) with respect to the deviations of the 50% fracture probability life $(N_{f 50%})$ were achieved.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.35 no.10
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• pp.1229-1235
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• 2011

Fatigue tests were conducted on the aluminum alloy, A7075-T6 to determine the most reliable fretting fatigue damage parameter. Specimens with grooves were used, so that either fretting fatigue crack at the pad/specimen interface or plain fatigue crack at the groove could be nucleated, depending on the pad pressure. Both the crack nucleation location and initial crack orientation were examined using optical microscopy, and the results were used to assess the reliability of the various fretting fatigue damage parameters that have been most commonly used in the literature. Finite element analysis was employed to obtain the stress and strain data of the specimen, which were needed to estimate the parameter values and the orientation of the critical plane. It was revealed that both the Fatemi.Socie and McDiarmid parameters, which assume shear-mode fatigue cracking, are the most reliable.

• Korean Journal of Microbiology
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• v.37 no.1
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• pp.49-55
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• 2001

Bacillus anthracis is a gram-positive spore-forming bacterium that causes the disease anthrax. The anthrax toxin contains three components, including the protective antigen (PA), which binds to eucaryotic cell surface receptors and mediates the transport of toxins into the cell. In this study, the entire 2,294-nucleotide protective antigen gene (pag) was sequenced from 4 of B. anthracis strains to identify potential variation in the toxin and to further our understanding of B. anthracis evolution in Korea. Sequence alignment of the entire PA gene from 30 strains representative of the four B. anthracis diversity groups revealed mutations. The mutation of B. anthracis BAK are located adjacent to a highly antigenic region crossing the junction between PA domains 3 and 4 shown to be critical to LF binding. The different mutational combinations observed in this study give rise to 11 PA genotypes and 4PA phenotypes. Three-dimensional analysis of all the amino acid changes (Ala to Val) observed in BAK indicated that these changes are not only close sequentially but also very close in three-dimensional space to the antigenic region importan tfor LF binding. Phylogenetic (cladistic) analysis of the pag corresponded with previous strain grouping based on chromosomal variation, suggesting that plasmid evolution in B. anthracis has occurred with little or no horizontal transfer between the different strains.

• Journal of the Korean Society of Safety
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• v.20 no.1 s.69
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• pp.36-41
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• 2005

PCV(Positive Crankcase Ventilation) valve acts as a flow control valve to get a re-combustion of blow-by gas by having it flow from a crankcase to an inlet manifold suction tube. The blow-by gas of the crankcase should be eliminated or taken properly because it cause corrosion to critical parts, and contributes to increase crankcase pressure that can cause a drop in efficiency. The excessive stress and strain on the PCV valve that remove these harmful gas would be bring the difficult on the flow rate control and failure of the valve. Those condition inevitably induce the accident. Therefore, this study purpose is FEM evaluation of the stresses and deformation in the X3 PCV model according to the change of the differential pressure between inlet and outlet. From results, the maximum equivalent stresses increased linearly according to the increase of the differential pressure at the about 50mm from the inlet position and were under the yield strength of the valve. And the deformations were relatively small regardless of the in-outlet differential pressure variation.

• Transactions of the Korean Society of Automotive Engineers
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• v.13 no.2
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• pp.1-9
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• 2005

An end beam is one of the most important structural members supporting uncovered freight under in-service loading. In general, it needs to endure over 25 years. However fatigue fracture has occurred at dynamic stress concentration location of the end beam because user's specifications demanded high speed and vehicle manufacturer made the uncovered freight car with comparatively low strength and stiffness. For durability analysis, finite element analysis is performed to evaluate the problem of uncovered freight structure and local strain. The uncovered freight car was operated on actual problematic railroad line to measure dynamic stress versus time history on the critical part from which a crack is initiated often. Rainflow cycle counting method was used to estimate fatigue damage at dangerous area under operating condition. Therefore, this study shows that analytical fatigue life at the end beam can be predicted on the basis of S-N curve and structure analysis and has a fairly good correlation with experimental fatigue life.

• Journal of Biosystems Engineering
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• v.36 no.3
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• pp.163-170
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• 2011

Analysis of load on major parts of the tractor power drive line is critical for efficient and optimum design of a tractor. The purpose of this study was to evaluate severeness of the tractor PTO driving axle during rotary tillage operation. First, S-N (stress vs. number of cycle) curve of a PTO driving gear was obtained through the fatigue life test using a PTO dynamometer. Second, PTO severeness was evaluated during rotary tillage operation. Torque measurement system was constructed with strain-gauge sensors to measure torque of a PTO axle, an I/O interface to acquire the sensor signals, and an embedded system to calculate severeness. The severeness of PTO was analyzed using measured torque data during rotary tillage. In the PTO gear life fatigue test, breakage time and bending stress of the gear were measured by tooth widths and torque change during the fatigue life test. The S-N curve showed a good linear relationship between bending stress and number of cycle (life) with a coefficient of determination of 0.97. For PTO severenss evaluation, rotary tillage operations were conducted at two PTO rotational speeds (level-1, level-2) under different paddy and upland field sites with different soil conditions. Results of averaged relative severeness for PTO level-1 and PTO level-2 were 1.96 and 3.34, respectively, at paddy field sites, and they were 1.36 and 2.51, respectively, at upland field sites. The results showed that the PTO driving axle experienced more severe load during rotary tillage at paddy fields than at upland sites, and relative severeness was greater at the higher PTO rotational speed under all of the soil conditions.

• Smart Structures and Systems
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• v.6 no.3
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• pp.183-196
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• 2010

Monitoring and economical design of alternative energy generators such as wind turbines is becoming increasingly critical; however acquisition of the dynamic output data can be a time-consuming and costly process. In recent years, low-cost wireless sensors have emerged as an enabling technology for structural monitoring applications. In this study, wireless sensor networks are installed in three operational turbines in order to demonstrate their efficacy in this unique operational environment. The objectives of the first installation are to verify that vibrational (acceleration) data can be collected and transmitted within a turbine tower and that it is comparable to data collected using a traditional tethered system. In the second instrumentation, the wireless network includes strain gauges at the base of the structure. Also, data is collected regarding the performance of the wireless communication channels within the tower. In both turbines, collected wireless sensor data is used for off-line, output-only modal analysis of the ambiently (wind) excited turbine towers. The final installation is on a turbine with embedded braking capabilities within the nacelle to generate an "impulse-like" load at the top of the tower. This ability to apply such a load improves the modal analysis results obtained in cases where ambient excitation fails to be sufficiently broad-band or white. The improved loading allows for computation of true mode shapes, a necessary precursor to many conditional monitoring techniques.