• Journal of Plant Biotechnology
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• 제38권4호
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• pp.241-250
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• 2011

식물유의 거의 대부분은 triacylglycerol (TAG) 형태로 종자에 축적되어있으며 이는 종자가 발아할 때에 필수적인 에너지공급원이자 동물과 인간들에게 필수지방산과 중요한 에너지원이다. 최근 식용유의 건강기능성으로 수요증가와 더불어 바이오디젤과 산업원료 등의 산업적 수요도 증가함에 따라 더욱 중요한 자원이 되고 있다. 그래서 생명공학기술로 종자유의 함량을 증진하고자 하면 지질 생합성에 탄소의 유입에 관여하는 조절 유전자를 과발현 또는 억제하는 것이 결정적으로 중요하다. 본 총설에서는 지질함량에 영향을 미치는 것으로 여겨지는 후보 유전자들에 대해 기술하고 이들의 지방 함량 증대 가능성을 조사하였다. 식물의 지방산의 생합성과 종자유의 축적에 관여하는 유전자들은 크게 구분하자면 첫째, TAG가 생합성되기 위해 필요한 전구체를 합성하는 유전자, 둘째, 지방산합성과 TAG 축적에 관여하는 유전자, 셋째, 종자 발달과 종자유 축적에 관여하는 전사인자 유전자가 있다. 종자유 함량을 결정하는 대사들은 앞에서 언급했듯이 매우 복잡하기 때문에 최근에 전사인자의 조절이 다수의 지방생산 대사 유전자를 동시 조작하여 형질전환 식물에서 종자유 함량이 증진하는 것보다 더 바람직한 접근법으로 여겨지고 있다. 그러나 전사조절유전자의 과발현에 의해 나쁜 농업형질의 유도 같은 문제점도 해결해야 한다.

• 한국가축번식학회지
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• 제7권2호
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• pp.57-71
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• 1983

1. Diluted chicken semen can be preserved at 2 to 5$^{\circ}C$ for 24 to 48 hr with resultant fertility of greater than 90% of that of fresh semen. Turkey semen can be preserved at 10 to 15$^{\circ}C$ for 6 to 24 hr and provide economical fertility. 2. Frozen chicken semen has given variable results; a 21 to 93% fertility ranges as compared to 92 to 94% expected with fresh semen. Highest fertility levels obtained with frozen turkey semen intravaginally inseminated have been 61 and 63% using DMSO and glycerol, respectively, as cryoprotectants. 3. The use of glycerol as a cryoprotectant reauires that its concentration in semen be reduced to less than 2% either by dialysis or centrifugation after thawing and before intravaginal insemination if optimal fertility is to be obtained. 4. The temperature at which cryoprotectants are added to semen and the time allowed for equilibration are important for subsequent fertility pre- and post-freezing. 5. The type of container used for packaging the semen, freeze or cooling rates, thaw rates and level of cryoprotectant all interact in affecting cell survival. 6. Plastic freeze straws as a packaging device for semen offers the following advantages: easy to handle, require minimal storage space, offer a wide range of freeze and thaw rates, and insemination can be made directly from them upon thawing. 7. Controlled slow cooling rates of 1 to 8$^{\circ}C$/min have thus far provided the best results for cooling chicken semen throught the transition phase change (liquid to solid) or critical temperature range of ＋5 to -20 or -35$^{\circ}C$. 8. Highest fertilities have been achieved with frozen chicken semen where a slow thaw rate (2。 to 5$^{\circ}C$) has been used regardless of the freeze rate. 9. To maintain a constant high level of fertility throughout a breeding season with frozen semen, a higher absolute number of spermatozoa must be inseminated (2 to 3 times as many) as compared to fresh semen since a, pp.oximately 50% are destroyed during processing and freezing. 10. The quality of semen may vary with season and age of the male. Such changes in sperm quality could be accentuated by storage effects. Thus, the correct number of spermatozoa may very well vary during the course of a breeding period. 11. As to time of insemination, it is best to avoid inseminating chicken hens within 1-2 hr after or 3-5 hr before oviposition; and turkey hens during or 7-10 hr before oviposition. 12. The physiological receptiveness of the oviduct at the time of insemination is a very important biological factor influencing fertility levels throughout the breeding season.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제35권1호
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• pp.31-37
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• 2013

Purpose: Maxilla and mandible have different patterns of cortical and trabecular bone and different bone mineral densities, even though both are components of the jaw bone. However, cellular differences between maxilla- and mandible derived osteoblasts (OBs) have rarely been studied. We hypothesize that maxilla- and mandible-derived OBs show different responses to $17{\beta}$-estradiol (E2), which is one of the critical factors for bone formation. This study compares skeletal site-specific cell responses between maxilla- and mandible-derived human OBs to E2. Methods: Maxilla- and mandible-derived OBs derived from an identical donor were separately isolated from a total of five normal healthy subjects aged 18~44 years old, cultured with a treatment of 100 nM estrogen. The responses between maxilla- and mandible-derived OBs to E2 were evaluated and compared using cell proliferation, alkaline phosphatase (ALP) activity and gene expression of osteoprotegerin (OPG), ALP, insulin-like growth factor-1 (IGF-1), and estrogen receptor ${\alpha}$ ($ER{\alpha}$). Results: E2 did not have any distinct effects on the proliferation of both types of OBs. Mandible-derived OBs exhibited higher ALP activity than maxilla-derived OBs in the non-treated condition, which was common in all tested individuals. ALP activities of both types of OBs showed a minor increasing tendency with the treatment of E2, even though there was no statistical significance in some specimens. The gene expression of OB by E2 was diverse, depending on the individuals. There was increased expression of OPG, IGF-1, or $ER{\alpha}$ gene in the part of subjects, which was more repeated in maxilla-derived OBs. In particular, OPG or ALP induction by E appeared less frequently in mandible-derived OBs. Conclusion: Current results revealed that E2 affects maxilla- and mandible-derived OBs into facilitating the osteogenic process despite individual differences. Mandible-derived OBs are less sensitive to bone-forming gene expression by E2.

• 한국재료학회지
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• 제19권1호
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• pp.28-32
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• 2009

Silicon carbide (SiC) is a promising material for power device applications due to its wide band gap (3.26 eV for 4H-SiC), high critical electric field and excellent thermal conductivity. The Schottky barrier diode is the representative high-power device that is currently available commercially. A field plate edge-terminated 4H-SiC was fabricated using a lift-off process for opening the Schottky contacts. In this case, Ni/Ti dual-metal contacts were unintentionally formed at the edge of the Schottky contacts and resulted in the degradation of the electrical properties of the diodes. The breakdown voltage and Schottky barrier height (SBH, ${\Phi}_B$) was 107 V and 0.67 eV, respectively. To form homogeneous single-metal Ni/4H-SiC Schottky contacts, a deposition and etching method was employed, and the electrical properties of the diodes were improved. The modified SBDs showed enhanced electrical properties, as witnessed by a breakdown voltage of 635 V, a Schottky barrier height of ${\Phi}_B$=1.48 eV, an ideality factor of n=1.04 (close to one), a forward voltage drop of $V_F$=1.6 V, a specific on resistance of $R_{on}=2.1m{\Omega}-cm^2$ and a power loss of $P_L=79.6Wcm^{-2}$.

• 대한통합의학회지
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• 제12권2호
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• pp.121-131
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• 2024

Purpose : This study aimed to investigate the leisure satisfaction of participants in the Geod-Jyu walking program and to examine the relationship between health promotion behaviors and quality of life. In addition, we investigated the impact of leisure satisfaction with walking among program participants on health promotion behaviors and quality of life. By clarifying these relationships, we aimed to identify factors of leisure satisfaction with walking that could enhance participants' health promotion behaviors and quality of life. Methods : A survey was conducted among 301 participants enrolled in the Geod-Jyu walking program in Chungcheongnam-do, with 288 responses analyzed. Data analysis included Pearson's correlation, confirmatory factor analysis, and multiple regression analysis using SPSS 27.0. Result : Leisure satisfaction exhibited a positive correlation with health promotion behavior (r=.544, p<.01) and quality of life (r=.478, p<.01). Furthermore, health promotion behavior showed a positive correlation with quality of life (r=.636, p<.01). Leisure satisfaction positively influenced quality of life, with physiological satisfaction (β=.16, t=2.32) and relaxation satisfaction (β=.15, t=2.04) emerging as notable contributors. In addition, leisure satisfaction had a significant positive impact on health promotion behaviors, with psychological satisfaction (β=.24, t=3.09) and educational satisfaction (β=.20, t=3.09) playing key roles. Health promotion behavior had a positive impact on quality of life, with all sub variables exhibiting significant positive effects in the following order: self-actualization (β=.24, t=4.16), stress management (β=.22, t=3.97), exercise (β=.22, t=4.05), and health responsibility (β=.12, t=2.14). Conclusion : The findings indicate that factors related to physical and rest satisfaction, as well as health-promoting behaviors facilitated by walking, significantly affected the quality of life among Geod-Jyu participants. Thus, promoting leisure satisfaction and engaging in health-promoting activities through walking can enhance overall well-being. Encouraging participation in the Geod-Jyu walking program is critical for physical and psychological benefits, and for promoting healthier behaviors. Further development of Geod-Jyu is expected to enhance walking satisfaction, promote self-care skills for health management, and positively influence quality of life.

• Journal of Preventive Medicine and Public Health
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• 제30권1호
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• pp.209-228
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• 1997

In ordo to investigate the professional job perception and job satisfaction of interns and residents and its related factors, the author conducted survey using self administered questionnaire for 2 months(August and September, 1996). The study subjects were 562 interns and residents who worked at one university hospital(tertiary hospital) and two general hospitals(secondary hospital) in Taegu, Korea. Among them, 297 (52.8%) responded completely. The results were as follows ; Respondents thought that people component was most important, science and status components were next among professional value scales which meant the desirable attitudes required to conduct professional works. But, the score of professional value scales was generally high and not affected by other variables in multiple linear regression analysis. They seemed to have very normative and ideal perceptions about professional values. The score of professionalism scales, which were attitudinal and behavioral traits about professional job, of high grade residents was significantly higher than that of interns and lower grade residents. The score of reference to professional organization and autonomy factor were increasing significantly as the grade increased. Working conditions and perception for socioeconomic status of doctors influenced the score of professionalism scale significantly. It seemed that professional socialization was made during the training periods of interns and residents. Most of respondents answered that current socioeconomic status of doctors were middle and high strata but they responded that the socioeconomic status of doctors would fall in the future. They seemed to have a pessimistic thought about doctor's status. Generally the respondents thought that they were satisfied with doctor job and fit to the job, but 51.9% answered that if possible they would get other jobs. It seemed to reflect their critical thinking on doctors' status. Perceptions about socioeconomic status of doctors, professional value and professionalism influenced job satisfaction significantly. The interns and residents had high sense of calling to doctors and thought that doctors were socially important job. Generally they were satisfied with their job. Interns and residents had normative thoughts about the trait which compentent doctors must have. During the training period, they seemed to have attitude and perception as a professional and to make professional socializations.

• 지질공학
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• 제21권4호
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• pp.281-293
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• 2011

이 연구에서는 과거 산사태의 이력이 있을 뿐만 아니라 추가적인 산사태가 예상되는 부산 황령산의 한 산사면에 대하여 산사태의 발달원인 및 특성에 대해 알아보기 위하여, 연구지역에 대한 (연도별)항공사진, 강우특성, 단열의 발달특성, GIS를 기반으로 한 지형분석, 그리고 수목들의 손상 및 기울어짐에 대한 통계학적 분석을 시도하였다. 그 결과, 북서-남동 방향의 사면파괴가 관찰되는 이 산사면은 1975년까지 비교적 안정한 상태를 유지하였으나, 1975년과 1985년 사이에 취약한 지질구조, 사면절개 및 집중호우와 같은 외적요인이 복합적으로 영향을 미쳐 비교적 큰 규모의 산사태가 발생하였음이 확인된다. 이는 도시형 산사태의 주요 원인 중 하나인 용지 및 택지개발을 위한 사변의 절취 시, 지질구조, 절취사면의 안식각 및 강우특성 동을 면밀히 검토하는 것이 중요함을 보여준다. 또한 기울어진 수목의 기울어진 방향과 각도에 대한 분석결과는 1985년 이후 낙석 및 포행의 형태로 추가적으로 진행되는 산사태가 주로 서쪽방향으로 진행되고 있으며, 산사면의 북서쪽 지점에 이들이 집중되고 있음을 지시한다. 이는 산사태 연구에 있어 손상되거나 변형된 수목을 이용한 분석방법이 매우 유용할 수 있음을 지시하고, 산사태의 예측 및 방재에도 적절히 활용될 수 있을 것으로 사료된다.

• 한국지반환경공학회 논문집
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• 제13권8호
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• pp.85-94
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• 2012

실내시험을 통하여 현장여건을 고려한 스미어 영향을 평가하기 위하여 3종류(C:M=1:0, C:M=0.5:0.5, C:M=0:1)의 재성형 시료를 이용하여 다양한 조건의 3차원 실내 모형시험을 30종 실시하여 스미어 존의 발생범위를 측정하고 대표적인 위치에서 시료를 채취하여 스미어 존 내에서의 투수계수의 변화특성을 검토하였다, 연구결과, C:M=1:0(점토 100%)의 경우 맨드렐 관입길이, 맨드렐의 크기, 토압고려여부, 단위중량 및 맨드렐의 형상의 순으로 스미어 존의 발생범위에 큰 영향을 미치는 것을 알았다. 따라서, 현장에서 맨드렐의 관입, 인발의 영향을 크게 받는 장척의 배수재를 설치하는 경우에는 기존의 실내시험을 통한 연구결과에 비해 스미어 존이 더욱 크게 형성되며, 스미어 존(smear zone)의 직경 ($d_s$)은 맨드렐(mandrel) 환산직경($d_m$)의 약 1.89~2.48배로 나타났다. 지반밀도와 맨드렐관입특성이 동일한 조건인 경우에 실트의 함유량이 높은 지반일수록 스미어 존의 발생범위는 넓어지는 것을 알았다. 현장의 토압이 크게 고려될수록, 맨드렐 사이즈가 클수록, 단위중량이 작을수록 스미어 존의 발생범위는 적게 나타났다. 맨드렐 관입, 인발에 의한 투수계수 비($k_{hs}/k_{ho}$)는 재성형시료를 사용한 본 실험에서 평균적으로 약 0.70~0.85의 범위를 나타냈으며, 투수계수저하의 영향요인과 영향치는 스미어 존의 발생범위와 유사하게 나타나는 것을 알았다.

• 의료ㆍ복지 건축 : 한국의료복지건축학회 논문집
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• 제22권4호
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• pp.37-45
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• 2016

Purpose: The purpose of this paper is to analyze a sample of single corridor, pod type and composite type of ICUs in terms of patient visibility, spatial configurations and nurse walking distance focused on Korean cases. Methods: The measures of static visibility were used to quantify the patient visibility (upper third part of the patient bed) from the nurse station. The measure of space programme and area distribution (patient zone percentage, staff zone percentage, circulation zone percentage, corridor length per bed, distance from nurse station (NS) to patient bed and departmental gross square meter (DGSM) per patient bed) were calculated by using AutoCAD and MS Excel programs. In the second step of analysis the values of space distribution were compared among the three type of ICUs as well as the nurse walking distance, DGSM per bed and gross factor. in the third step of analysis regression analysis was conducted for the possible correlation between visibility and space programme and area distribution factors as well as nurse walking distance factors. Results: (1) It was found that on average composite type unit offer highest value of patient visibility followed by pod type, while single corridor type unit offers the minimum value of patient visibility among the three plan typologies. (2) Average patient visibility and DGSM per bed shows a strong positive correlation ($r^2=0.75$) and p=0.026. (3) Average patient visibility and average distance from NS has a strong negative correlation ($r^2=0.78$), and P=0.02. (4) On average composite type unit offer the minimum walking distance from NS (7.22 meter) followed by pod type unit (8.35 meter) and single corridor (9.76 meter). (5) Maximum distance from NS was noted in single corridor (18.19 meter) followed by pod type unit (15.14 meter) and Composite type unit (11.1 meter). Implications: This study may contribute to the visibility analysis of existing and future ICU design in Korea to achieve maximum patient visibility and reduced nurse walking distance.

• 생명과학회지
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• 제32권3호
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• pp.241-248
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• 2022

NF-κB는 염증과 선천성 면역에 중요한 전사인자이며 anti-apoptotic gene을 유도하여 세포의 생존과 발암에도 깊이 연관되어 있으며 많은 신호전달분자 및 신호전달회로와 연결되어있다. 한편, Hsp90는 NF-κB의 활성을 조절한다는 보고가 이루어졌으나 그 구체적인 기전은 알려져 있지 않다. 본 연구에서는 IKK compelx를 구성하는 인자들의 발현 plasmid를 이용하여 NF-κB의 활성조절에서 Hsp90와 IKKγ의 연관성 및 역할을 연구하였다. 그 결과 Hsp90는 IκBα의 인산화와 분해를 촉진하여 NF-κB를 활성화시켰고, NIK과 LPS에 의한 NF-κB의 활성화는 Hsp90에 의해 더욱 활성이 증가하였다. IKKγ는 Hsp90에 의해 증가된 IκBα의 인산화와 분해를 더욱 촉진함으로써 Hsp90의 NF-κB 활성화 작용을 상승시켰다. 이러한 Hsp90와 IKKγ에 의한 NF-κB의 활성화 현상은 항상 활성화 된 상태를 유지하는 IKKβ-EE mutant를 이용한 검토에서도 입증되었다. 또한 IKKγ의 deletion mutant를 이용한 검토에서 IKKγ의 N-말단에 위치하는 IKKβ 결합부위와 C-말단에 위치하는 leucine zipper 및 zinc finger 부위는 IKKγ와 Hsp90의 NF-κB에 대한 상호협력적 촉진작용에 필요하지 않았다. 또한 Hsp90에 의해 촉진된 세포내 pro-inflammatory cytokine들의 발현은 IKKγ에 의해 더욱 상승하였다. 이러한 결과로부터 Hsp90와 IKKγ의 상호작용을 차단한다면 NF-κB의 과다활성으로 인한 질병의 예방과 치료에 도움을 줄 수 있을 것으로 사료된다.