• The Plant Pathology Journal
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• 제21권1호
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• pp.47-54
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• 2005

Identification of host genes involved in disease progresses and/or defense responses is one of the most critical steps leading to the elucidation of disease resistance mechanisms in plants. Soybean mosaic virus (SMV) is one of the most prevalent pathogen of soybean (Glycine max). Although the soybeans are placed one of many important crops, relatively little is known about defense mechanism. In order to obtain host genes involved in SMV disease progress and host defense especially for virus resistance, two different cloning strategies (DD RT-PCR and Subtractive hybridization) were employed to identify pathogenesis- and defenserelated genes (PRs and DRs) from susceptible (Geumjeong 1) and resistant (Geumjeong 2) cultivars against SMV strain G7H. Using these approaches, we obtained 570 genes that expressed differentially during SMV infection processes. Based upon sequence analyses, differentially expressed host genes were classified into five groups, i.e. metabolism, genetic information processing, environmental information processing, cellular processes and unclassified group. A total of 11 differentially expressed genes including protein kinase, transcription factor, other potential signaling components and resistant-like gene involved in host defense response were selected to further characterize and determine expression profiles of each selected gene. Functional characterization of these genes will likely facilitate the elucidation of defense signal transduction and biological function in SMV-infected soybean plants.

• Journal of Ecology and Environment
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• 제38권2호
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• pp.133-143
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• 2015

Freshwater macrophytes improve the structural heterogeneity of microhabitats in water, often providing an important habitat for zooplankton. Some studies have focused on the overall influence of macrophytes on zooplankton, but the effects of macrophyte in relation to different habitat characteristics of zooplankton (e.g., epiphytic and pelagic) have not been intensively studied. We hypothesized that different habitat structures (i.e., macrophyte habitat) would strongly affect zooplankton distribution. We investigated zooplankton density and diversity, macrophyte characteristics (dry weight and species number), and environmental parameters in 40 shallow wetlands in South Korea. Patterns in the data were analyzed using a self-organizing map (SOM), which extracts information through competitive and adaptive properties. A total of 20 variables (11 environmental parameters and 9 zooplankton groups) were patterned onto the SOM. Based on a U-matrix, 3 clusters were identified from the model. Zooplankton assemblages were positively related to macrophyte characteristics (i.e., dry weight and species number). In particular, epiphytic species (i.e., epiphytic rotifers and cladocerans) exhibited a clear relationship with macrophyte characteristics, while large biomass and greater numbers of macrophyte species supported high zooplankton assemblages. Consequently, habitat heterogeneity in the macrophyte bed was recognized as an important factor to determine zooplankton distribution, particularly in epiphytic species. The results indicate that macrophytes are critical for heterogeneity in lentic freshwater ecosystems, and the inclusion of diverse plant species in wetland construction or restoration schemes is expected to generate ecologically healthy food webs.

• 한국의학물리학회지:의학물리
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• 제27권4호
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• pp.213-219
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• 2016

The position verification of the radiation source utilized in brachytherapy forms a critical factor in determining the therapeutic efficiency. Currently, films are used to verify the source position; however, this method is encumbered by the lengthy time interval required from film scanning to analysis, which makes real-time position verification difficult. In general, the source position accuracy is usually tested in a monthly quality assurance check. In this context, this study investigates the feasibility of the real-time position verification of the radiation source in high dose rate (HDR) brachytherapy with the use of scintillating fibers. To this end, we construct a system consisting of scintillating fibers and a silicon photomultiplier (SiPM), optimize the dosimetric software setup and radiation system characteristics to obtain maximum measurement accuracy, and determine the relative ratio of the measured signals dependent upon the position of the scintillating fiber. According to the dosimetric results based on a treatment plan, in which the dwell time is set at 30 and 60 s at two dwell positions, the number of signals is 31.5 and 83, respectively. In other words, the signal rate roughly doubles in proportion to the dwell time. The source position can also be confirmed at the same time. With further improvements in the spatial resolution and scintillating fiber array, the source position can be verified in real-time in clinical settings with the use of a scintillating fiber-based system.

• 터널과지하공간
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• 제24권4호
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• pp.282-288
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• 2014

스토핑 채광법은 현재 가장 보편적으로 이용되고 있는 지하 금속광 채광법 중 하나이다. 그러나 채광과정에서 발생하는 확정선외 혼입은 광산 전반의 생산성을 떨어뜨리며 때때로 폐광의 주요 원인을 제공하기도 하여 그 예측과 관리가 시급한 실정이다. 이를 위해 본 연구는 서 호주의 한 지하광산에서 조사한 자료를 바탕으로 인공 신경망(ANN)을 이용하여 보다 신뢰성 있는 확정선외 혼입 예측모델을 제시하였다. 또한, 연결가중 알고리즘(CWA)에 의한 요인분석을 통해 확정선외 혼입의 영향인자에 대한 기여도 분석을 실시하였다. 제안된 확정선 외 혼입 예측 ANN 모델의 학습과 시험 단계의 상관계수는 0.9641과 0.7933으로 강한 상관관계를 보였으며, CWA분석결과 발파공 길이(BHL)과 절리 방향 안전계수(SFJ)의 기여도가 18.78%와 17.99%로 다른 인자에 비해 비교적 중요한 영향인자 임을 확인할 수 있었다.

• 항공우주시스템공학회지
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• 제12권2호
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• pp.66-75
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• 2018

본 논문에서는 2MW급 풍력 블레이드의 스파캡을 탄소복합재료, 두께축소율(PRR) 및 상쇄연구(Trade-off study)를 이용해서 경량화 설계를 수행했다. 블레이드 스파캡은 블레이드의 기계적 건전성을 결정하는 가장 핵심적인 요소이다. 가벼우면서도 기계적 신뢰성을 확보할 수 있는 블레이드 스파캡의 형상을 도출하기 위해 주어진 설계하중으로 스파캡의 두께를 변화시키면서 반복적인 구조해석을 실시한다. 파손여부를 판정하기 위해서 Tsai-Wu 및 Puck 파손이론을 사용하였으며, 그 결과 GFRP 복합재료보다 CFRP 복합재료가 동일한 조건에서 약 30% 무게를 경량화 할 수 있었다. 해석 결과를 바탕으로 복합재료 적층두께의 최적값을 도출하여 구조적 성능 향상 및 경량화 된 설계 결과를 제시한다.

• 한국동물위생학회지
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• 제27권4호
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• pp.355-369
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• 2004

The equine herpesvirus type 1 (EHV-1) immediate-early (IE) protein is a potent transactivator responsible for the activation of both early and late genes during the course of infection and is comprised of discrete functional domains that mediate its many functions. Interaction between trans activators such as the IE protein and various components of the RNA polymerase II transcription initiation machinery has been demonstrated to be critical for transactivation. In the present report, it is addressed the hypothesis that the IE protein interacts with various components of transcription machinery to mediate transactivation of target viral genes. In these studies, it is demonstrated that in vitro transcribed and translated IE protein interacts with TFIIB-agarose conjugate but not with TFIID-agarose conjugate. Additional immunoprecipitation studies using nuclear extracts derived from EHV-1 infected RK-13 cells confirmed that the IE protein interacts strongly with TFIIB, but fails to interact with TFIID. IR2, a truncated form of the IE protein lacking the potent transactivation domain and involved in the down-regulation of the IE gene, also interacted with TFIIB but not with TFIID. Studies were also performed to ascertain if particular TBP-associated factors (TAFs) could mediate IE or IR2 binding to TFIID. In vitro transcribed and translated TAF250 added to nuclear extracts generated from EHV-1 infected cells also failed to mediate an interaction between the IE protein or the IR2 protein and TFIID. This study demonstrated that the IE protein mediates transactivation of target viral genes by a mechanism that involves TFIIB. This is in contrast to mechanisms that have been proposed for both the herpes simplex virus ICP4 and VP16 protein which have been proposed to transactivate viral genes through interactions involving both TFIIB and TFIID. This study also intimates that IR2 mediate its repressive effects during the course of EHV-1 infection by a mechanism that involves sequestration of various transcription factors.

• 대한기계학회논문집A
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• 제38권2호
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• pp.121-127
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• 2014

단층 그래핀 시트(Single layer graphene sheet, SLGS)의 찢어짐 모드(모드 III) 파괴 예측을 위한 원자 기반 미세결합요소모델이 개발되었다. 이 모델은 그래핀 시트의 최대 변형률 관계를 예측하기 위해 수정된 모스포텐셜을 사용한다. 면외 전단하중 조건에서 그래핀의 모드 III 파괴를 광범위한 분자역학(Molecular mechanics, MM) 시뮬레이션으로 조사하였다. 분자역학은 원자의 균열선단 근처 원자의 변위를 설명하기 위해 사용되었고, 선형탄성파괴역학은 이 영역 바깥의 영역을 설명하기 위해 사용되었다. 해석 결과 분자역학 방법이 SLGS의 전단 물성 계산뿐만 아니라 armchair 및 zigzag 방향 모드 III 파괴인성 연구에도 단순하면서도 신뢰할만하다는 것을 보여준다. SLGS 의 모드 III 파괴인성은 zigzag 방향에 대해 $0.86MPa{\sqrt{m}}$, armchair 방향에 대해 $0.93MPa{\sqrt{m}}$로 예측되었다.

• Nuclear Engineering and Technology
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• 제37권1호
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• pp.91-100
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• 2005

Kyung-hee Thorium Fuel (KTF), a heterogeneous thorium-based seed and blanket design concept for pressurized light water reactors, is being studied as an alternative to enhance proliferation resistance and fuel cycle economics of PWRs. The proliferation resistance characteristics of the KTF assembly design were evaluated through parametric studies using neutronic performance indices such as Bare Critical Mass (BCM), Spontaneous Neutron Source rate (SNS), Thermal Generation rate (TG), and Radio-Toxicity. Also, Fissile Economic Index (FEI), a new index for gauging fuel cycle economy, was suggested and applied to optimize the KTF design. A core loaded with optimized KTF assemblies with a seed-to-blanket ratio of 1: 1 was tested at the Korea Next Generation Reactor (KNGR), ARP-1400. Core design characteristics for cycle length, power distribution, and power peaking were evaluated by HELIOS and MASTER code systems for nine reload cycles. The core calculation results show that the KTF assembly design has nearly the same neutronic performance as those of a conventional $UO_2$ fuel assembly. However, the power peaking factor is relatively higher than that of conventional PWRs as the maximum Fq is 2.69 at the M$9^{th}$ equilibrium cycle while the design limit is 2.58. In order to assess the economic potential of a heterogeneous thorium fuel core, the front-end fuel cycle costs as well as the spent fuel disposal costs were compared with those of a reference PWR fueled with $UO_2$. In the case of comprising back-end fuel cycle cost, the fuel cycle cost of APR-1400 with a KTF assembly is 4.99 mills/KWe-yr, which is lower than that (5.23 mills/KWe-yr) of a conventional PWR. Proliferation resistance potential, BCM, SNS, and TG of a heterogeneous thorium-fueled core are much higher than those of the $UO_2$ core. The once-through fuel cycle application of heterogeneous thorium fuel assemblies demonstrated good competitiveness relative to $UO_2$ in terms of economics.

• 디지털융복합연구
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• 제19권4호
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• pp.89-101
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• 2021

창업 초기 창업가의 역량이 미진하고, 자원이 풍부하지 않으면서 네트워크에 한계가 있는 스타트업은 지속적 스케일업이 어렵다. 본 연구는 ICT 스타트업 중에서 스케일업에 성공한 �� 스타트업을 사례로 선정하여 ERIS 모델을 기반으로 ICT 스타트업이 성공하는 첫 단계에서 발생하는 스케일업 과정에 대한 사례분석을 진행했다. 분석결과, 창업가의 경험적 역량, 다양한 산업 간의 지식의 융합, 공공기관 주도의 연구개발 과제의 참여, 고객과 기업간의 소통 채널 운영, 프로젝트형 캠페인의 활용이 ICT 스타트업의 스케일업에 중요하게 작용하는 것으로 나타났다. 학술적으로 본 연구는 ERIS 모델이 향후 ICT 스타트업의 스케일업과 신시장 기업 성공요인 분석 모델로써 의미가 있음을 확인 및 검증한 것에 그 의미가 있다. 또한 실무적으로는 ICT 스타트업의 초기 시장 경쟁력을 확보하고 스케일업을 하기 위한 전략적 전개의 참조사례로 활용될 수 있을 것이다.

• 대한토목학회논문집
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• 제26권5C호
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• pp.323-331
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• 2006

지반의 저항인자인 침식특성을 정량화하여 이를 세립토 지반의 세굴심 산정에 반영하는 것은 세계적인 추세이다. 이러한 노력의 일환으로 국내에서 많은 장대 해상교량이 계획 또는 시공되고 있는 서해안 지역 세립토 지반의 침식특성을 실험을 통해 분석하였다. 대상지역으로는 인천대교 현장, 강화 초지대교 현장, 환경교 현장, 군산 장항지구 등 4곳을 선정하였으며, 총 34개의 불교란시료를 채취하여 세굴률 실험을 수행하였다. 지반의 침식능을 나타내는 한계전단응력은 지반의 비배수전단 강도와 비례하는 경향을 보였으며, 점성을 가진 세립토가 세굴에 대한 저항이 보다 큰 것으로 나타났다. 실험을 통해 지역별 침식특성을 분석 및 체계화하여 서해안 지역 장대교량 건설시 보다 정확한 세굴심도 산정 및 기초 설계를 위한 기초자료로 제공하고자 한다.