• 한국지형학회지
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• 제28권1호
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• pp.101-114
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• 2021

Junior Ranger is a self-directed interpretation program for young visitors at national parks in the United States. The success of this program can be largely attributed to the role of an activity book which is given an applicant free of charge at a visitor center. This study aims to analyze the main characteristics of activity books for 14 national parks' Junior Ranger and to draw some implications for Korean geomorphological education. Although the activity books are varied in size, volume and printing, all of them offer diverse activities which are composed of different contents related to park resources in four fields and are performed in different ways such as Q&A, picture and word game, and creative activity. The time-consuming activities including attendance at a ranger-led program prevent the participant from making a superficial visit to be a junior ranger. The implications of the study are as follows: Firstly, the geomorphological education for children is needed to be strongly based on field experience learning and to be more carried out using a way of game rather than conventional Q&A, suggesting that it encourages students not to lose their interest for learning. Secondly, it is also necessary for the learning contents to be focused on various resources related to landform as well as landform itself. In addition, a creative activity such as writing verse or drawing feeling should be more applied to the geomorphological education in order to enhance their effects on affective domain beyond cognitive one. It is likely to be an alternative approach to understand landform by internalizing a sense of landform.

• Journal of Microbiology and Biotechnology
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• 제31권2호
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• pp.189-196
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• 2021

Periplanetasin-2 from cockroach exhibits broad-spectrum antimicrobial activity. The underlying antibacterial mechanisms rely on the stimulation of reactive oxygen species overproduction to induce apoptotic cell death. A promising strategy to increase the bioavailability of periplanetasin-2 involves reducing the dose through combination therapy with other antibacterials that show synergistic effects. Thus, the synergistic antibacterial activity of periplanetasin-2 with conventional antibacterial agents and its mechanisms was examined against Escherichia coli in this study. Among the agents tested, the combinations of periplanetasin-2 with vancomycin and chloramphenicol exhibited synergistic effects. Periplanetasin-2 in combination with vancomycin and chloramphenicol demonstrated antibacterial activity through the intracellular oxidative stress response. The combination with vancomycin resulted in the enhancement of bacterial apoptosis-like death, whereas the combination with chloramphenicol enhanced oxidative stress damage. These synergistic interactions of periplanetasin-2 can help broaden the spectrum of conventional antibiotics. The combination of antimicrobial peptides and conventional antibiotics is proposed as a novel perspective on treatments to combat severe bacterial infection.

• Journal of Microbiology and Biotechnology
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• 제34권7호
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• pp.1395-1400
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• 2024

The antimicrobial activity of the natural compounds from plant and food have well discovered since the interest on the beneficial effect of the natural compounds was risen. Quercetin, a flavonoid derived from vegetables, including onions, red leaf lettuces and cherries has been studied for diverse biological characteristics as anti-cancer and anti-microbial activities. The aim of current study is to investigate the specific antibacterial modes of action of quercetin against Escherichia coli. Quercetin decreased the E. coli cell viability and induced the severe damages (oxidative stress, DNA fragmentation) leading to cell death. Reactive oxygen species (ROS) generation was observed during the process, which we confirmed that oxidative stress was the key action of antibacterial activity of quercetin exerting its influence potently. Based on the results of Annexin V and Caspace FITC-VAD-FMK assay, the oxidative damage in E. coli has led to the bacterial apoptosis-like death in E. coli. To sum up, the contribution of ROS generation exerts crucial impact in antibacterial activity of quercetin.

• BMB Reports
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• 제32권6호
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• pp.547-553
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• 1999

Aminoacyl-tRNA synthetases are essential enzymes catalyzing the attachment of specific amino acids to cognate tRNAs. In the present work, the substrate analogue L-methionine hydroxamate was used to identify functional residues located in the active site of the E. coli methionyl-tRNA synthetase (MetRS). This compound inhibited bacteria, yeast, and human MetRS activities to a similar degree, suggesting a conserved active site structure and mechanism between MetRSs of different phylogenetic domains. Mutants of the E. coli MetRS resistant to methionine hydroxamate were also isolated. These mutants contained a substitution either at T10, Y15, or Y94. These residues are highly conserved among the different MetRSs and the mutants showed decreased aminoacylation activity, suggesting their functional and structural significances. The putative roles of these residues are discussed on a structural basis.

• 대한지구과학교육학회지
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• 제5권1호
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• pp.114-123
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• 2012

This study investigates the effect of environmental club activities of the creative experiential learning activities on the improvement of students' Environmental Literacy and Pro-environmental Behavior. For that purpose, This study divided 24 students at Jinhae U Elementary School into test groups of 12 students and Control groups of 12 students. Test groups participated in the 'GomGomi' environmental Club for sixteen times from March 2011 to December 2011. Conclusions of this study include; First, we found that the environmental club activities is effective to improve the students' environmental Literacy overall. Specifically, the Environmental Club Activities was effective in the sub-catagories of environmental Literacy such as 'rights of nature' and 'eco-crisis'. However, there was no significant change in the sub-category of 'human exemptionalism' Second, we found that the environmental club activities is effective to improve students' pro-environmental behavior overall. Specifically, the environmental club activities was effective in the sub-domains of Pro-environmental Behavior such as 'cognitive domain' and 'affective domain'. However, there was no significant change in the sub-domains of 'behavioral domain'. Summary, the environmental club activities was shown to be effective for improving their environmental literacy and pro-environmental behavior; This study implies that the environmental club activities of the creative experiential learning activities would be a effective tool to help students to improve their environmental literacy and pro-environmental behavior.

• 한국환경교육학회지:환경교육
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• 제23권1호
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• pp.50-63
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• 2010

This study is a qualitative case study aiming at understanding and finding out influential factors and barriers when school teachers choose Environmental Education as their Creative Discretionary Activities. To do this, this study focuses on the process of two teachers' choices of the Creative Discretionary Activities in two other elementary schools. The results of this research are follows. Two teachers' choices are affected by several influential factors, divided into external factors and internal factors. External influential factors include culture of school and education, media, chief education officers, parents and school. Internal influential factors are teachers' value and philosophy of education, teachers' experiences, teachers' capacity. These influential factors' forces aren't the same with each other. These influential factors, are influenced by educational, social, national, local, and school level indicated by Taylor et al's (1974) classification criterion. Especially, however, Korea's particular educational environment focusing on university entrance examination strongly affects teachers' choice. School factors also has a big strong effect on teachers' choice. Among others internal actors are the most serious influential factor affecting on teachers' choice.

• 한국정보통신학회논문지
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• 제19권6호
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• pp.1353-1358
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• 2015

창조경제는 산업화시대, 정보화시대, 지식기반경제를 잇는 새로운 경제 패러다임이며, 창조경제의 핵심 키워드는 창의성, 혁신성, 소비자, 지식재산권 보호 및 활용이다. 창의적인 아이디어가 소프트웨어 기술과 결합하여 Apple, Google, Facebook 과 같은 혁신적인 생태계 기반의 시장을 구축한 것이 대표적인 사례라고 하겠다. 이는 창조성에 기반을 둔 지적자산으로 경제적 가치의 중요성이 높게 인식되고, 지식정보 사회를 뛰어넘는 경제성장 동력으로 혁신적 창조성과 아이디어가 요구되고 있다. 이것의 핵심에는 과학과 문화 산업이 융합되어진 과학기술과 업 그리고 문화예술 등 다양한 장르가 융합되어진 콘텐츠 산업의 육성으로 창조경제의 핵심인 동시에 경제 활성화에 기여하기 때문이다. 본 연구에서는 미국의 스미스소니언협회의 사례를 분석하여 창조적인 인력 양성을 위한 과학관의 방향성을 제시 하고자 한다.

• 중소기업융합학회논문지
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• 제4권2호
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• pp.19-24
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• 2014

최근 정부는 '창조경제(Creative Economy)'를 통하여 상상력과 창의성, 과학기술에 기반한 경제 운용, 새로운 시장, 새로운 일자리 창출을 목표로 하고 있다. 이는 또한 과학기술과 ICT, 문화, 인문학 등이 융합하여 산업간 경계부분에서 창조경제의 성과를 이룩할 수 있다는 것이 선진국의 창조경제 벤치마킹에서 증명되고 있다. 특히, 여성 과학기술인이 창조형 선진국의 사례를 보면 중요한 역할을 수행하고 있다. 하지만 한국은 여성 과학기술인이 낮은 수준이고 전문적인 여성과학기술자에 대한 적극적인 지원 및 육성정책도 미흡한 것으로 알려져 있다. 또한 경제 활동 중이던 여성들은 육아와 출산 등으로 인한 경력단절, 복귀에서 겪는 어려움 때문에 30대 초 중반 여성이 경제활동에 참여하는 비율이 적다. 창조경제를 지속적으로 발전시키기 위해서는 이를 이끌어갈 여성 과학기술인의 창조적 교육과 육성, 지원에 힘을 기울여야 한다. 따라서 본고에서는 창조경제 시대의 ICT 산업정책과 여성의 역할과 경제활동 참여 현황, 그리고 창조경제시대의 여성 일자리 창출방안과 문제점을 살펴본다.

• 인지과학
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• 제21권3호
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• pp.409-427
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• 2010

창의성과 지식의 관계를 설명하는 심리학적인 연구는 크게 긴장적 관점과 토대적 관점의 두 관점이 적용되어 오고 있으나, 기존의 연구들은 대부분 창의적 수행 결과를 토대로 한 창의적 산물 중심의 연구로서 창의적 사고 과정에 관한 정보를 제공해주는 데에는 한계점을 지니고 있다. 본 연구에서는 사고 과정에 관한 정보를 제공해줄 수 있는 뇌과학적 기법을 이용하여 지식과 창의성의 관계를 규명하고자 10명의 컴퓨터 전문가를 선정하여 언어적 창의성 과제인 소설쓰기 과제를 제시 한 후 전문 영역과 비전문 영역의 창의적 사고 과정 시 뇌파(EEG) 알파파의 과제 관련 파워값(Task Related Power, TRP)을 비교하였다. 실험은 기준 상태로 눈 감고 안정 상태, 일반적 사고 과정, 전문 분야의 창의적 사고 과정, 비전문 분야의 창의적 사고 과정 시의 EEG를 측정한 후 각각 비교 분석하는 순서로 진행되었다. 연구 결과 전문 영역의 창의적 사고 과정 시 알파파의 활성이 유의하게 감소함을 알 수 있었으며 이러한 현상이 주로 측두엽에서 나타났고, 특히 좌측 측두엽에서 뚜렷하게 관찰됨을 알 수 있었다. 이러한 관찰 결과는 지식이 창의적 사고를 방해할 수 있다는 긴장적 관점을 지지하는 것으로 창의적 사고 과정에 관한 연구를 뇌과학적으로 접근할 수 있음을 시사한다.

• International Journal of Oncology
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• 제54권4호
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• pp.1327-1336
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• 2019

Endothelial progenitor cells (EPCs) are bone marrow (BM)-derived progenitor cells that can differentiate into mature endothelial cells, contributing to vasculogenesis in the blood vessel formation process. Runt-related transcription factor 3 (RUNX3) belongs to the Runt domain family and is required for the differentiation of specific immune cells and neurons. The tumor suppressive role of RUNX3, via the induction of apoptosis and cell cycle arrest in a variety of cancers, and its deletion or frequent silencing by epigenetic mechanisms have been studied extensively; however, its role in the differentiation of EPCs is yet to be investigated. Therefore, in the present study, adult BM-derived hematopoietic stem cells (HSCs) were isolated from Runx3 heterozygous (Rx3^+/-) or wild-type (WT) mice. The differentiation of EPCs from the BM-derived HSCs of Rx3^+/- mice was found to be significantly increased compared with those of the WT mice, as determined by the number of small or large colony-forming units. The migration and tube formation abilities of Rx3^+/- EPCs were also observed to be significantly increased compared with those of WT EPCs. Furthermore, the number of circulating EPCs, defined as CD34⁺/vascular endothelial growth factor receptor 2 (VEGFR2)⁺ cells, was also significantly increased in Rx3^+/- mice. Hypoxia-inducible factor (HIF)-1α was upregulated in Rx3^+/- EPCs compared with WT EPCs, even under normoxic conditions. Furthermore, in a hindlimb ischemic mouse models, the recovery of blood flow was observed to be highly stimulated in Rx3^+/- mice compared with WT mice. Also, in a Lewis lung carcinoma cell allograft model, the tumor size in Rx3^+/- mice was significantly larger than that in WT mice, and the EPC cell population (CD34⁺/VEGFR2⁺ cells) recruited to the tumor was greater in the Rx3^+/- mice compared with the WT mice. In conclusion, the present study revealed that Runx3 inhibits vasculogenesis via the inhibition of EPC differentiation and functions via the suppression of HIF-1α activity.