• Journal of Life Science
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• v.20 no.9
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• pp.1294-1298
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• 2010

White spot syndrome virus (WSSV) is one of the most virulent viral agents threatening the penaeid shrimp culture industry. This study was carried out to evaluate the susceptibility of the sand shrimp, Crangon affinis, to WSSV as an alternative experimental model. WSSV caused 100% mortality in C. affinis within 7 days after experimental infection by immersion. Based on challenge studies, it was confirmed that C. affinis could be a potential host in WSSV transmission. Also, the neutralization of WSSV was carried out using an antiserum raised against recombinant envelop protein rVP466 to evaluate the WSSV infection mechanism. A constant amount of WSSV (at $1{\times}10^4$ diluted stocks) was incubated with various amounts of antiserum and then mixed to 20 l reservoir for the immersion challenge of C. affinis for neutralization. At 5 days post challenge, the shrimp in the positive control immersed in the immersion reservoir containing WSSV stock showed 100% mortality. The shrimps challenged with the 3 different mixtures of WSSV and rVP466 antiserum (1:0.1, 1:0.5 and 1:1) showed 100%, 68.8% and 68.8% mortality at 14 days post challenge, respectively. These results indicated that the antiserum raised against rVP466 could block WSSV infection in C. affinis. Therefore, this study confirmed that C. affinis can be naturally infected by WSSV as another potential host and that C. affinis can be used as an alternative experimental animal instead of penaeid shrimps.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.22 no.5
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• pp.351-362
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• 1989

Ecology of Crangon affinis was studied in the Nakdong River Estuary fron June 1988 to May 1989. The growth rate of female was similar to that of male. Size differences, however, were apparent between female and male. Sex ratio varies with season. The major spawning season was from June 1988 to May 1989 except for November 1988. The number of eggs of the largest ovigerous female (35.55mm TL) was over 4,000, while that of the smallest ovigerous female (15.05mm TL) more than 120. The mean number of eggs was 1778. The size of winter eggs is larger than that of the summer eggs. The mean percentage of egg loss from brooding to hatching was $29.06\%$. The rate of egg loss decreases with increasing age. Major component of food organisms includes mysids and polycheates.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 1996.06a
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• pp.136-137
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• 1996

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.6
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• pp.784-790
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• 1999

Species composition and seasonal variation of a shrimp assemblage in the coastal waters of Kori, Korea were studied based on the monthly trawled samples through a year of 1996. In the study period, 11 species of shrimps from 6 families were collected. Of these Crangon affinis predominated throughout the year. The peak abundances occurred in June and November, and low ones in winter and early autumn. More species were collected in winter and spring than summer and autumn. Species diversity indices showed that the shrimp assemblage was more diverse in winter than spring and autumn. Predominance of C. affinis in June, July and November caused high abundances and low diversity indices. The shrimps in the study area can be grouped into two groups on the basis of their occurrence patterns: resident species and seasonal species.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.25 no.5
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• pp.371-382
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• 1992

Biochemical composition, the rates of nitrogen excretion in the form of ammonia, amino acids and total nitrogen, and oxygen consumption of the shrimp Crangon affinis were measured at three temperature regimes $(7,\;15,\;and\;25^{\circ}C)$ and the variations were also determined for starvation periods (1-25 days). The composition of the major biochemical fractions was analysed into carbohydrate: $4.2\%,\;protein:\;68.6\%,\;lipid:\;7.0\%,\;chitin:\;6.3\%,\;and\;ash:\;14.6\%,$ all expressed as percentage of dry weight. Molting frequency was lower at $7^{\circ}C\;than\;25^{\circ}C$ during the period of starvation, and during the same period the higher temperature was, body weight and body compositions the more decreased. Through all starvation periods $O_2$ consumption tended to decrease but total nitrogen tended to increase at any temperature regimes. The dominant form of excreted total nitrogen was ammonia-N at any temperature. From the O:N ratio it appeared that carbohydrate and lipid reserves were quickly exhausted (1-5 days), and that proteins were the substrates oxidized to meet the energetic requirements of C. affinis at any temperature. After 25 days of starvation the O:N ratio remained constant near a value of 8, which indicates that only proteins were being utilized at three temperatures. After 25 days of starvaion C. affinis excreted 23.01ug N/mg body nitrogen per day at $7^{\circ}C,\;32.97\mu g\;N/mg$ body nitrogen per day at $15^{\circ}C,\;and\;44.81\mu g\;N/mg$ body nitrogen per day at $25^{\circ}C$, and lost about 1.75, 2.47 and $3.29^{\circ}C$ of body protein per day at 7, 15, and $25^{\circ}C$ respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.4
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• pp.532-542
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• 1997

Seasonal variation of a shrimp community in the eelgrass (Zostera marina) bed in Kwangyang Bay, Korea was studied based on the monthly collected samples through a year of 1994. The shrimp community in the eelgrassbed was composed of 26 species of shrimps representing 6 families. The community was dominated by Heptacarpus pandaloides, Crangon affinis, Eualus leptognathus, Latreutes acicularis, Heptacarpus rectirostris, Heptacarpus geniculatus, and Latreutes laminirostris. Most of species were the small-sized species with carapace length smaller than 25 mm. The peak abundance occurred in the late winter and spring and low abundances in autumn. More than 10 species were collected every month except lull (8 species). Species diversity indices showed that the shrimp species were more diverse during the late summer, and lesser during the late autumn. Predominance of Crangon affinis in the late autumn caused such a low diversity. The shrimps in the study area could be grouped into three groups on the basis of their occurrence patterns: resident species, seasonal species, and temporary species. More abundant and more diverse shrimps were collected during nighttime than daytime.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.22 no.5
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• pp.342-350
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• 1989

Maturity and spawning of Crangon affinis were studied based on the histological observation. The samples were monthly collected in Nakdong estuary from June, 1988 to May, 1989. The gonad lies on the dorsal side of the thorax. The cavity in which it lies is located below heart and above hepatopancreas. Anterior part of ovary is fused roundly, and the posterior part shows a pair of tubule-like structure. Testis is bilaterally symmetrical; the anterior part shows convoluted tubule, and the posterior part consists of a pair of tubule. Seminal vesicle is connected and opened to the base of the fifth pereiopod. Gametogenesis of ovum and spermatozoon is being repeated in short period without seasonality, and it is formed simultaneously in the whole parts of germinal epithelium. Diameter of a ripe oocyte is ca. $430{\mu}m$. Spermatozoa is oval with distinct nucleoplasm. Reproductive cycle is performed in ca. 40 days. Crangon affinis spawns all year round except November. Incubation period of brooded eggs took $12\~14$ days before hatching in the aquarium at $21^{\circ}C\~24^{\circ}C\;and\;33\%_{\circ}$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.43 no.5
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• pp.521-531
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• 2010

This study examined the community structure and temporal variation of the macro-crustaceans on Jangbong tidal flat, Incheon, Korea. Macro-crustaceans on this tidal flat were collected using a modified otter trawl from November 1999 to January 2001. The macro-crustaceans comprised 32 species, 7,741 individuals, and 29,211.3 gWWt during the study period. The Jangbong tidal flat was dominated numerically by three shrimp (Palaemon carinicauda, Crangon affinis, and Metapenaeus joyneri) and two portunid crabs (Charybdis japonica and Portunus trituberculatus). The macro-crustacean assemblages were divided into two different season and species groups based on the q-mode and r-mode clustering methods. In terms of q-mode, they consisted of a winter species group from December through April and a summer species group from May through November. The r-mode analysis showed that the species group visiting the tidal flat in winter was dominated numerically by palaemonid and crangonid shrimps, while a species group characterized by an abundance of portunid crabs and penaeid shrimps was more frequent in spring, summer, and autumn. In conclusion, our study revealed distinct temporal variation in the tidal flat use by macro-crustaceans around Jangbong Island, Incheon, Korea.

• Korean Journal of Ecology and Environment
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• v.55 no.4
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• pp.368-375
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• 2022

Gut contents analysis is essential to predict the impact of organisms on food source changes due to variations of the habitat environment. Previous studies of gut content analysis have been conducted using traditional methods, such as visual observation. However, these studies are limited in analyzing food sources because of the digestive process in gut organ. DNA metabarcoding analysis is a useful method to analyze food sources by supplementing these limitations. We sampled marine fish of Pennahia argentata, Larimichthys polyactis, Crangon affinis, Loligo beka and Sepia officinalis from Gwangyang Bay and Yeosu fisheries market for analyzing gut contents by applying DNA metabarcoding analysis. 18S rRNA v9 primer was used for analyzing food source by DNA metabarcoding. Network and two-way clustering analyses characterized the relationship between organisms and food sources. As a result of comparing metabarcoding of gut contents for P. argentata between sampled from Gwangyang Bay and the fisheries market, fish and Copepoda were analyzed as common food sources. In addition, Decapoda and Copepoda were analyzed as common food sources for L. polyactis and C. affinis, respectively. Copepoda was analyzed as the primary food source for L. beka and S. officinalis. These study results demonstrated that gut contents analysis using DNA metabarcoding reflects diverse and detailed information of biological food sources in the aquatic environment. In addition, it will be possible to provide biological information in the gut to identify key food sources by applying it to the research on the food web in the ecosystem.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.1
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• pp.78-84
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• 2011

We investigated the stomach contents of finless porpoises collected in the Yellow Sea. Prey organisms in the stomachs of 109 finless porpoises were identified as 11 species of fish, 8 species of shrimp and 4 species of cephalopod. Index of Relative Importance analysis revealed that the porpoises fed mainly on the Japanese sand shrimp (Crangon affinis). The energy density of the prey was estimated to be 5.46 kJ. The daily energy requirement was estimated to be 18,051 kJ/day for a porpoise with an average weight of 32.49 kg. The estimated annual consumption by finless porpoises in the Yellow Sea was 25,454 tons. The average commercial catch in the Yellow Sea by Korean fisheries was 135,913 tons from 2005 to 2009. Assuming that the abundance of finless porpoises is stable, porpoises ate approximately 18.7% of the commercial catch. The fishery catch may be influenced by the consumption by finless porpoises, while fishery bycatch is a cause of porpoise mortality in the Yellow Sea.