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http://dx.doi.org/10.11614/KSL.2022.55.4.368

Analysis of Stomach Contents of Marine Orgnaisms in Gwangyang Bay and Yeosu Fish Market Using DNA Metabarcoding  

Gun Hee Oh (Department of Ocean Integrated Science, Chonnam National University)
Yong Jun Kim (Department of Ocean Integrated Science, Chonnam National University)
Won-Seok Kim (Department of Ocean Integrated Science, Chonnam National University)
Cheol Hong (Department of Ocean Integrated Science, Chonnam National University)
Chang Woo Ji (Fisheries Science Institute, Chonnam National University)
Ihn-Sil Kwak (Department of Ocean Integrated Science, Chonnam National University)
Publication Information
Korean Journal of Ecology and Environment / v.55, no.4, 2022 , pp. 368-375 More about this Journal
Abstract
Gut contents analysis is essential to predict the impact of organisms on food source changes due to variations of the habitat environment. Previous studies of gut content analysis have been conducted using traditional methods, such as visual observation. However, these studies are limited in analyzing food sources because of the digestive process in gut organ. DNA metabarcoding analysis is a useful method to analyze food sources by supplementing these limitations. We sampled marine fish of Pennahia argentata, Larimichthys polyactis, Crangon affinis, Loligo beka and Sepia officinalis from Gwangyang Bay and Yeosu fisheries market for analyzing gut contents by applying DNA metabarcoding analysis. 18S rRNA v9 primer was used for analyzing food source by DNA metabarcoding. Network and two-way clustering analyses characterized the relationship between organisms and food sources. As a result of comparing metabarcoding of gut contents for P. argentata between sampled from Gwangyang Bay and the fisheries market, fish and Copepoda were analyzed as common food sources. In addition, Decapoda and Copepoda were analyzed as common food sources for L. polyactis and C. affinis, respectively. Copepoda was analyzed as the primary food source for L. beka and S. officinalis. These study results demonstrated that gut contents analysis using DNA metabarcoding reflects diverse and detailed information of biological food sources in the aquatic environment. In addition, it will be possible to provide biological information in the gut to identify key food sources by applying it to the research on the food web in the ecosystem.
Keywords
gut contents; DNA metabarcoding; 18S rRNA; feeding; fish;
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