• Journal of The Korean Society of Grassland and Forage Science
• /
• v.36 no.2
• /
• pp.89-97
• /
• 2016

This study was carried out to determine the effect of mixed pasture using domestic varieties orchardgrass 'Kodione' and tall fescue 'Purumi' on forage yields and botanical composition from 2013 to 2015 in middle region of Korea. The following mixed pastures were used in this study: treatment A (orchardgrass oriented mixture with imported varieties), treatment B (orchardgrass oriented mixture), treatment C (orchardgrass and tall fescue oriented mixture), treatment D (tall fescue oriented mixture), treatment E (tall fescue oriented mixture without orchardgrass), treatment F (only orchardgrass), and treatment G (only tall fescue). In botanical compositions at last cutting time in 2015, treatment A had orchardgrass at 7.1%, tall fescue at 47.9%, kentucky bluegrass at 15.2% and white clover at 25%. Meanwhile, treatment B had orchardgrass at 25.9%, tall fescue at 47.0%, kentucky bluegrass at 4.9% and white clover at 18.3%. Consequentially, in botanical composition from 2014 to 2015, treatment A was changed more compare to other treatments B, C, D and E. The average of dry matter (DM) yield for 2 years of treatment E (18,369 kg/ha) was the highest among the seven treatments, but there was no significant difference among other treatments except treatment A (p>0.05). The crude protein (CP) and in vitro dry matter digestibility (IVDMD) of treatment A were 14.5% and 74.8%, and treatment E were 14.1% and 73.0%, respectively. The content of neutral detergent fiber (NDF) and acid detergent fiber (ADF) were lower in treatment E and A than other treatments, and treatment E showed high content of total digestible nutrient (TDN). Therefore, tall fescue oriented mixture using domestic tall fescue variety Purumi had good forage productivity, quality and botanical composition in middle region of Korea for the establishment of grassland.

• Food Engineering Progress
• /
• v.15 no.4
• /
• pp.346-354
• /
• 2011

This study was carried out to fortify the antimicrobial activity of yoghurt by adding liquorice extract to it. The liquorice extracts (1 mg/mL) showed relatively high antibacterial activity against H. pylori KCCM 40449 (p < 0.05). The solvent liquorice extracts of minimal inhibitory concentrations (MIC) against H. pylori KCCM 40449 were 25- 100 ${\mu}g$/mL. Lactobacillus amylovorus DU-21 with high EPS production ability were inoulated to milk after the addition of different amounts of liquorice extracts (0.0%, 0.05%, 0.1% and 0.2%). The physico-chemical characteristics of yoghurts added with liquorice extracts were examined. The initial pH, titratable acidity, viscosity and viable cell counts of the yoghurt added liquorice extracts were 3.41-3.51, 1.021-1.091%, 1,686-1,930 cp and 9.41-9.38 Log CFU/mL, respectively. The viscosity and syneresis of yoghurt were better than that of the control. Antimicrobial activity against H. pylori KCCM 40449 increased with increasing addition of liquorice extract. However, the sensory score of yoghurt added with different amounts of liquorice extracts was lower than that of the control (p < 0.05). As a result of the sensory evaluations, the flavor, taste, texture, color and overall acceptability of the yoghurt with 0.05% liquorice extract were found to be much better than those of the other groups (p < 0.05). Overall, the optimal amount of liquorice extract added in the manufacture of yoghurt was 0.05% of the total weight. Further studies on increment of antimicrobial activity and palatability of liquorice extract added yoghurt are necessary.

• Journal of the Korea Concrete Institute
• /
• v.19 no.1
• /
• pp.121-130
• /
• 2007

To investigate the enhancement in strength and deformation capacities of concrete confined by FRP composites, tests under axial loads were carried out on three groups of thirty six short columns in circular section with diverse GFRP confining reinforcement. The major test variables considered include fiber content or orientation, wrap or tube type by varying the end loading condition, and continuous or discontinuous confinement depending on the presence of vortical spices between its two halves. The circumferential FRP strains at failure for different types of confinements were also investigated with emphasis. Various analytical models capable of predicting the ultimate strength and strain of the confined concrete were examined by comparing to observed results. Tests results showed that FRP wraps or tubes provide the substantial increase in strength and deformation, while partial wraps comprising the vertical discontinuities fail in an explosive manner with less increase in strength, particularly in deformation. A bilinear stress-strain response was observed throughout all tests with some variations of strain hardening. The failure hoop strains measured on the FRP surface were less than those obtained from the tensile coupons in all tests with a high degree of variation. In overall, existing predictive equations overestimated ultimate strengths and strains observed in present tests, with a much larger scatter related to the latter. For more accuracy, two simple design- oriented equations correlated with present tests are proposed. The strength equation was derived using the Mohr-Coulomb failure criterion, whereas the strain equation was based on entirely fitting of test data including the unconfined concrete strength as one of governing factors.

• Microbiology and Biotechnology Letters
• /
• v.43 no.2
• /
• pp.105-111
• /
• 2015

An alginate degrading enzyme from the Vibrio crassostreae PKA 1002 strain was used to hydrolyze the water extract of Sargassum thunbergii. To obtain the optimum degrading conditions for the S. thunbergii water extract, the mixture of the water extract and enzyme was incubated at 30℃ for 0, 3, 6, 12, and 24 h, and its alginate degrading ability was measured by reducing sugar and viscosity. A temperature of 30℃ for a period of 6 h was found to be the optimal condition for the enhancement of the alginate’s degrading ability. The pH of the enzymatic hydrolysate was not significantly different from that of the water extract. Overall lightness decreased, but redness and yellowness increased after enzymatic hydrolysis. Total phenolic compounds did not differ between the water extract and the enzymatic hydrolysate. DPPH radical scavenging activity and the reducing power of the enzymatic hydrolysate were lower than those of the water extract. However, the chelating effect of the enzymatic hydrolysate (80.08% at 5 mg/ml) was higher than that of the water extract (62.29%). These results indicate that the enzymatic hydrolysate possesses an anti-oxidant activity by way of the action of the chelating effect.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.19 no.2
• /
• pp.115-120
• /
• 1999

This experiment was carried out to investigate the effects of additives on the quality of alfalfa(Medicago sativa L.) silage at the forage experimental field, National Livestock Research Institute, RDA, Suwon in 1996. The experiment was arranged in a randomized block design with three replications. The treatments consisted of different additives(control, formic acid, molasses, inoculant A and inoculant B). Crude protein(CP) and Nitrogen free extract(NFE) content of alfalfa silage with additives were higher compared with those obtained control, but Crude ash(CA) and Crude fiber(CF) content of control(11.5 and 39.6%) were higher than those of additive treated plots. ADF and NDF contents of control silage were the highest as 36.2 and 48.6%, respectively, and increased by 4% compared with those obtained before ensiling. The acidity(pH) of control was the highest(5.45), but the lowest in inoculant A(4.32). Inoculant teratment significantly reduced acidity of silage compared with control. DM content of control silage was the lowest but DM loss of control silage was higher than that of additive treated silage. Ammonia-N content was the highest at formic acid treatment and level of Lactobacillus was decreased at control and formic acid treated silage. Total organic acid and lactic acid content of inoculant A and inoculant B were higher than those of other treated plots. The results of this study indicate that the treatment of LAB(Lactic acid bacteria) could be recommended as producing high quality of alfalfa silage.

• Proceedings of the Korea Contents Association Conference
• /
• 2003.05a
• /
• pp.344-350
• /
• 2003

In this paper, we propose a novel sharing method ordering the events occurring between users collaborated with the common telecomputing environment. We realize the sharing method with multimedia data to improve the coworking effect using teleprocessing network. This sharing method advances the efficiency of communicating projects such as remote education, tele-conference, and co-authoring of multimedia contents by offering conveniences of presentation, group authoring, common management, and transient event productions of the users. As for the conventional sharing white board system, all the multimedia contents segments should be authored by the exclusive program, and we cannot use any existing contents or program. Moreover we suffer from the problem that ordering error occurs in the teleprocessing operation because we do not have any line-up technology for the input ordering of commands. Therefore we develop a method of retrieving input and output events from the windows system and the message hooking technology which transmits between programs in the operating system In addition, we realize the allocation technology of the processing results for all sharing users of the distributed computing environment without any error. Our sharing technology should contribute to improve the face-to-face coworking efficiency for multimedia contents authoring, common blackboard system in the area of remote educations, and presentation display in visual conference.

• Molecules and Cells
• /
• v.38 no.3
• /
• pp.210-220
• /
• 2015

Athletic performance is an important criteria used for the selection of superior horses. However, little is known about exercise-related epigenetic processes in the horse. DNA methylation is a key mechanism for regulating gene expression in response to environmental changes. We carried out comparative genomic analysis of genome-wide DNA methylation profiles in the blood samples of two different thoroughbred horses before and after exercise by methylated-DNA immunoprecipitation sequencing (MeDIP-Seq). Differentially methylated regions (DMRs) in the pre-and post-exercise blood samples of superior and inferior horses were identified. Exercise altered the methylation patterns. After 30 min of exercise, 596 genes were hypomethy-lated and 715 genes were hypermethylated in the superior horse, whereas in the inferior horse, 868 genes were hypomethylated and 794 genes were hypermethylated. These genes were analyzed based on gene ontology (GO) annotations and the exercise-related pathway patterns in the two horses were compared. After exercise, gene regions related to cell division and adhesion were hypermethylated in the superior horse, whereas regions related to cell signaling and transport were hypermethylated in the inferior horse. Analysis of the distribution of methylated CpG islands confirmed the hypomethylation in the gene-body methylation regions after exercise. The methylation patterns of transposable elements also changed after exercise. Long interspersed nuclear elements (LINEs) showed abundance of DMRs. Collectively, our results serve as a basis to study exercise-based reprogramming of epigenetic traits.

• Korean Journal of Poultry Science
• /
• v.30 no.1
• /
• pp.17-28
• /
• 2003

A feeding trial was carried out to evaluate the effects of feeding Lactobacillus reuteri culture(LRC) on the performance, nutrients digestibility, intestinal microflora, serum metabolites, ammonia generation and litter dampness in broiler chicks. Four hundred eighty, one day old male chicks were fed into none, 0.1, 0.2 and 0.4% of LRC supplementation fer seven weeks. Basal diets excluded antibiotics contained ME 3,100, 3,100 kcal/kg, and CP 22.0, 20.0% for starter and grower, respectively. Weight gain of chicks fed LRC was significantly higher than no supplemental group in overall period(P<0.05). Feed intake was the highest in the 0.1% LRC, but not statistically different from other treatments. Feed conversion showed no significance among treatments. Viable Lactobacillus spp. number of chicks fed 0.2 and 0.4% LRC was significantly higher in cecum at seven weeks of age compared to the none(P<0.05). The tendency of anaerobes number was similar to Lactobacillus spp in ileum and cecum. Total number of E. coli and Salmonella were no difference in all treatments. In serum metabolites, feeding LRC increased triglyceride, and inorganic phosphorus, but no different total protein, albumin, total cholesterol, glucose, blood urea nitrogen and Ca. Nutrients digestibility improved significantly in 0.4% LRC compared to that of none(P<0.05). Fecal NH$_3$, gas generation was greatly decreased in the LRC supplemental groups(P<0.05). Moisture contents of bedding was also significantly decreased in LRC feeding group. It was concluded from the present study that feeding Lactobacillus reuteri culture improved the growth performance and nutrients digestibility of broiler chicks and minimize the fecal noxious gas emission.

• Korean journal of food and cookery science
• /
• v.20 no.4
• /
• pp.342-351
• /
• 2004

Rice flour was roasted at different temperatures and times and tarakjuk was made from these roasted rice flours. Chemical composition, in vitro starch digestibility (IVSD) and protein digestibility (IVPD) of the roasted rice flours and tarakjuk were determined. Changes in quality characteristics such as pH, viscosity, IVSD and IVPD of tarakjuk during refrigerated storage were also investigated. For roasted rice non, the protein content ranged from 6.52∼7.13% on a dry basis, while for tarakjuk, the range was 3.74 ∼4.0%. On the other hand, the Ca level of tarakjuk ranged from 1,278.36∼1,340.87 ppm. Rice flours showed decreasing IVSD and increasing IVPD on roasting at 145$^{\circ}C$ and 165$^{\circ}C$, whereas they showed increasing IVSD and decreasing IVPD at 185$^{\circ}C$, respectively. Roasting also influenced the pH, viscosity, IVSD md IVPD of tarakjuk made from these roasted rice flours. As the roasting temperature and time increased, tarakjuk showed lower pH and viscosity, however it showed higher IVSD and IVPD. The pH of tarakjuk, except that of control, decreased with storage, whereas viscosity increased significantly. IVSD decreased up to 4 days of storage, after which it increased again until 14 days of storage. On the other hand IVPD increased up to 7 days of storage, but there was no additional significant increase after 14 days of storage. These results suggest that depending on the nutritional purpose, appropriate conditions for roasting of rice flour and storage of tarakjuk may be recommended for the commercialization of tarakjuk.

• Korean Journal of Poultry Science
• /
• v.44 no.2
• /
• pp.113-122
• /
• 2017

This study was conducted to investigate the effect of dietary supplementation with Lactobacillus, medicinal plant extracts, and plant extracts on growth performance, cecal microflora, relative organ weight, and lymphocyte profile in broiler chickens. One hundred broilers (5 wk old) were used and divided into five (treatment) ${\times}$ two (challenged Salmonella) groups containing 10 broilers. The treatments were as follows: no antibiotics group (NC), antibiotics group (PC), 0.1% lactic acid bacteria group (LB), 100 ppm medicinal plant extract group (MPE), and 100 ppm herb extract group (HE). A basal diet was formulated as 3,100 kcal/kg ME and 20% CP based on corn and soybean meal. Broilers were fed with the experimental diets with no challenge for 1 wk and with challenge of SG for 2 wk. SG were added in water as $1.0{\times}10^6cfu/L$. Final body weight and weight gain were significantly decreased following challenge with SG (P<0.05). However, with the addition of antibiotic alternatives, growth performance was improved and reduction of performance following challenge with SG was lowed compared with that of the NC treatment (P<0.05). Coliform bacteria and Salmonella but not lactic acid bacteria increased with the addition of antibiotic alternatives (P<0.05). Lactobacillus increased significantly with the addition of lactic acid bacteria compared with the NC and PC treatments (P<0.05). The weight of liver, spleen, and bursa of Fabricius increased with addition of antibiotic alternatives (P<0.05). WBC was highly reduced at 4 days after challenge with SG, but was normally maintained thereafter. There was no significant difference in coliform bacteria, Salmonella, organ weights, or WBC with the addition of antibiotic alternatives. Finally, Lactobacillus, medicinal plant extract, and herb extract can be used as antibiotic alternatives; but one alternative completely can't alternate as antibiotics. Therefore, further studies are needed to investigate the synergistic effects of two or more antibiotic alternatives.