• Title/Summary/Keyword: Cow's milk

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Measurement of Lactoferrin, IgA, IgG1, IgG2, Antibacterial Activity, and Lactic Acid Bacterial Growth in Holstein Colostrum (Holstein 초유 중 Lactoferrin, IgA, IgG1, IgG2 정량과 미생물의 성장에 미치는 영향)

  • Renchinthand, Gereltuya;Bae, Hyoung-Churl;Nam, Myoung-Soo
    • Food Science of Animal Resources
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    • v.27 no.4
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    • pp.522-530
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    • 2007
  • This experiment was carried out to measure the content of lactoferrin, IgA, $IgG_1,\;IgG_2$, in Holstein colostrum, and to test the effect of it's colostrum on the antibacterial activity to pathogenic bacteria and the growth stimulation of lactic acid bacteria. Colostrum was collected at the first, second, and third day after parturition in summer and winter season. The levels of lactoferrin, IgA, $IgG_1,\;and\;IgG_2$ in Holstein cow colostrum were 0.30 mg/mL, 0.37 mg/mL, 4.00 mg/mL, 0.37 mg/mL, respectively, on the first day of the summer season whereas they were 1.16 mg/mL, 2.60 mg/mL, 13.35 mg/mL, 1.30 mg/mL on the first day of the winter season, postpartum. Heat treated ($65^{\circ}C$ for 30 min) or non-treated colostrum showed antibacterial activity toward Escherichia coli. The growth of commercial mixed strains (Bifidobacterium longum, Lactobacillus acidophilus, Streptococcus themophilus), L. acidophilus, L. casei, L. bulgaricus, and L. lactis subsp. cremoris were improved in first, second and third day colostrum compared to normal milk. Commercial miked strains (B. longum, L. acidophilus S. themophilus) lowered the pH to 4.97-5.22 and 4.89 while increasing the titratable acidity to 0.75-0.88% and 0.70% in colostrum and normal milk, respectively. However, L. casei, L. bulgaricus, L. lactis subsp. cremoris lowered the pH to 5.96-6.47 and 6.5-6.8 while increasing the titratable acidity to 0.29-0.48% and 0.20-0.25% in colostrum and normal milk, respectively.

Increase of Production Ratio of Pre-selected Superior Dairy Female Offspring by Combination of OPU derived Oocytes and X-bearing Semen

  • Kim, Seong-Su;Choi, Byung-Hyun;Lee, Kyeong-Lim;Jin, Jong-In;Suh, Tae-Kwang;Son, Cheol-Ho;Park, Chan-Ho;Shin, Seung-Oh;Han, Kwang-Jin;Lim, Hyun-Tae;Cho, Kyu-Woan;Kong, Il-Keun
    • Journal of Embryo Transfer
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    • v.30 no.2
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    • pp.73-82
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    • 2015
  • This study was designed to evaluate the possibility of increase through dairy female offspring's ratio by transfer of pre-selected transferrable blastocyst that was produced by pre-selected X-bearing semen with OPU derived oocytes. Elite dairy female cow is demanded strongly compared with male, the so called, farmer wants to produce only an elite female dairy offspring as a candidate female dairy cow for producing milk. In our study, we selected 2 elite dairy bull semen from National Agricultural Cooperative Federation to pre-select X-bearing semen and 5 elite dairy female cows as donor for collecting of OPU derived oocytes. OPU derived embryo production system was carried out an aspiration of immature oocytes from 5 donor cows 2 times per week, total 200 times for 2 to 7 months by an ultrasonographic guided follicular aspiration system and then produced in vitro-produced blastocysts by in vitro maturation, fertilization and culture. Dairy donor semen selected H-319, 320 bull in National Agricultural Cooperative federation was sorted X-bearing semen by flow-cytometer and frozen for using IVF with OPU derived oocytes. Donor cows were selected 5 elite dairy cows from Gyeongju Dairy Cow Community and then disease tests such as 4 kinds of disease before selecting was checked. Oocyte proportion of grade 1 to 3 from total collected oocytes was significantly lower in donor A and B than those in donor C, D and E (82.16 and 70.03% vs. 90.0, 91.78 and 93.57%), respectively (p<0.05). However, number of oocytes per session in donor A, C and E was significantly higher than those in donor B and D ($7.77{\pm}3.26$, $5.85{\pm}2.10$ and $7.03{\pm}2.14$ vs. $4.68{\pm}2.61$ and $5.21{\pm}1.97$ oocytes), but donor A was significantly higher than donor C (p<0.05). Development to blastocyst in donor B, C and E was significantly higher than those in donor A and D (31.0, 25.0 and 25.0% vs. 14.3 and 4.5%), but donor A was not different in donor C and E (p<0.05). Nine out of 10 blastocysts (90.0%) derived from OPU blastocysts were confirmed male embryos that was induced with Y-bearing semen to confirm sex ratio only. Total 96 blastocysts derived from female bearing semen were transferred into synchronized recipients and then confirmed 42 recipients (43.8%) pregnancy rate, 36 offspring (37.5%) and 91.7% female sex ratio (33 female vs. 3 male offspring). Taken together all data, elite dairy female offspring could be produced effectively by in vitro production system between pre-selected x-bearing semen and OPU derived oocytes that would be influential breeder in the breeding of dairy farm to increase effectively elite dairy offspring ratio as well as net income in the dairy farmer.

Studies on Dairy Farming Status, Reproductive Efficiencies and Disorders in New Zealand (I) A Survey on Dairy Farming Status and Milk Yield in Palmerston North Area (뉴질랜드 (Palmerston North) 의 낙농 현황과 번식 및 번식장해에 관한 연구(I) Palmerston North 지역의 낙농 현황과 우유 생산량에 관한 조사 연구)

  • 김중계;맥도날드
    • Korean Journal of Animal Reproduction
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    • v.24 no.1
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    • pp.1-18
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    • 2000
  • Eighty dairy farms in Palmers ton North area in New Zealand were surveyed on 1) general characteristics (10 Questions), 2) milk yield and feed supplementary (7 questions), 3) reproductive efficiencies (12 questions) and 4) reproductive disorders (12 questions) by mail questions from February to July, 1998. Among those 4 items from 38 dairy farms (47.5%), especially in items 1) and 2), overall dairy farming situation, supplementary feeding and milk yields were surveyed and analyzed for Korean dairy farmers (especially in Cheju island) to have better understanding or higher economical gains. The results were as follows. 1. In dairy experience, 21 (45%) among 38 dairy farms surveyed were answered that farming less than 15 years, 15~19 year, 20~25 years and over 26 years experience were 3 (7.9%), 7 (18.4%), 6 (15.8%) and 5 (13.2%) which generally showed longer experience compare to Korean dairy farming situation. In survey of labour input and business goal of dairy farming, self-managing farms, sharemilkers, unpaid family manpowering farms, manager running farms, farms with hired worker, farms with part time helper and other type was 21 (55.3%), 10 (26.3%), 2 (3.5%), 3 (5.3%), 18 (31.6%), 2 (3.5%), and 1 (1.8%), respectively. 2. Analyzing pasture and tillable land, pasture according to feeding scale (200, 300 and 400 heads) were 56, 90 and 165.3 ha, and tillable lands were 51, 78 and 165 ha which showed some differences among feeding scale. In recording methods in 38 farms replied, 36 (95%) dairy handbook and 23 (70%) dual methods taking farms were higher than that of 10 (26.3%) computer and 15(39.5%) well-recorder methods. 3. Dairy waste processing facilities in environmental field were almost perfect except of metropolitan area, and so no problem was developed in its control so far. Hence, 26 farm (68.4%) of pond system was higher rather than those in 8 (21.2%) of using as organic manure after storing feces of dairy cattle, 1(2.6%) bunker system and 3 (7.9%) other type farms. 4. In milking facilities, 33 farms (86.9%) of Harringbone types were higher than those in 3 (7.9%) of Walkthrough types, 1 (2.6%) of Rotary system and other types. Although the construction facilities was not enough, this system show the world-leveled dairy country to attempted to elevate economic gains using the advantage of climatic condition. 5. In milking day and yearly yield per head, average 275 milking days and 87 drying days were longer than that of 228 average milking days in New Zealand. Annual total milk yield per head and milk solid (ms) was 3,990 kg and approximately 319 kg. Dairy milk solid (ms) per head, milk yield, fat percentage was 1.2 kg, 15.5 kg and average 4.83% which was much higher than in other country, and milk protein was average 3.75%. 6. In coclusion, Palmerstone North has been a center of dairy farming in New Zealand for the last 21 years. Their dairy farming history is 6~9 year longer than ours and the average number of milking cows per farm is 355, which is much greater than that (35) of Korea. They do not have dairy barn, but only milking parlors. Cows are taken care of by family 0.5 persons), are on a planned calving schedule in spring (93%) and milked for 240~280 days a year, avoiding winter. Cows are dried according to milk yield and body condition score. This management system is quite different from that of Korean dairy farms. Cows are not fed concentrates, relying entirely on pasture forages and the average milk yield per cow is 3,500 kg, which is about 1/2 milk yield of Korean dairy farms. They were bred to produce high fat milk with an average of 4.5%. Their milk production cost is the lowest in the world and the country's economy relies heavily on milk production. We Korean farmers may try to increase farming size, decreasing labor and management costs.

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A Study on Why Breastfeeding be Interrupted and Knowledge of Breastfeeding (일부 지역에 거주하는 영아어머니의 모유수유의 중단이유와 모유수유 지식정도에 관한 조사연구)

  • Lee, Seon-Ah;Choi, So-Young
    • Journal of Korean Academic Society of Home Health Care Nursing
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    • v.9 no.1
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    • pp.27-36
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    • 2002
  • The purpose of this study was to investigate the reason of interruption and the knowledge of breastfeeding. The samples of 117 mothers who have the babies between age from 1 month to 12 months were selected from one university hospital and one public health care center in J City. The data were collected with the questionnaire during the period from the January. 5. 2002 through January. 30. 2002. Analysis was done by using frequency. percentage. t-test. ANOVA. The results of this study can be summarized as follows. 1. The comparison of the method of feeding by general characteristics. income state(p= .019), rearing supporters(p=.026) and infant age(p=.03) have shown significant difference as the variables affecting breastfeeding practice rate. Breastfeeding rate were 24.8%. mixed- feeding type, 14.5%. milkfeeding type. 60.7%. 2. The major reason which breastfeeding be done was 'for health of baby'. In the period of breastfeeding, a large number of mothers answered' up to. 6 months'. The major reason of breasttfeeding interruption was 'insufficient quantity of breastmilk'. 3. The mean of knowledge on breastfeeding by feeding patterns were the highest in the breastfeeding group(F= 10.228, p= .000). 4. In a grade of each item on knowledge of breastfeeding, over 80% of mothers wrongly answered in two items; components comparison between breastmilk and cow's milk and when the mother fell sick. whether she have to be feed breastmilk. or not. In the conclusion. this study revealed that knowledge of breastfeeding promoted practice of breastfeeding. However we suggest that systemic and supportive breastfeeding program for promoting the knowledge and the practice rate of breastfeeding have to be provided by nurses.

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Relationship Between the Incidence of Displaced Abomasum and Feeding of Cows in Tokachi District, Hokkaido, Northern Japan

  • Mori, F.;Sawada, K.;Watanabe, K.;Ducusin, R.J.T.;Kumase, N.;Tanabe, S.;Uzuka, Y.;Takahashi, J.;Sarashina, T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.1
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    • pp.88-91
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    • 2001
  • In order to investigate the relationship between the incidence of displaced abomasum and feeding, the actual feeding practices and chemical compositions of roughage were examined in 2 kinds of farms at Tokachi district in Hokkaido. Examination of animal health records revealed that the annual milk yield per cow in high incidence (H-DA) farms was significantly higher than that in low incidence (L-DA) farms. The amount of concentrates fed in H-DA farms tended to be higher than that in L-DA farms during lactation. Compared to L-DA farms, the amount of juicy roughage (corn silage and grass silage) and dry roughage (hay, hay cube and roll wrap silage) during lactation in H-DA farms tended to be higher and lower, respectively. Moreover, the amount of roughage and the ratio of roughage to concentrates in H-DA farms tended to be lower than in L-DA farms. The survey indicated that displaced abomasum was associated with insufficient feeding of dietary fiber and overfeeding of concentrates.

Induction of Regulatory Dendritic Cells by Lactobacillus paracasei L9 Prevents Allergic Sensitization to Bovine β-Lactoglobulin in Mice

  • Yang, Jing;Ren, Fazheng;Zhang, Hao;Jiang, Lu;Hao, Yanling;Luo, Xugang
    • Journal of Microbiology and Biotechnology
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    • v.25 no.10
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    • pp.1687-1696
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    • 2015
  • Supplementation with probiotics can protect against the development of food allergies by modulating the host immune response; however, the mechanisms are not fully understood. The objective of this study was to investigate the allergy-reducing effects of regulatory dendritic cells (regDCs) induced by Lactobacillus paracasei L9 (L9) in β-lactoglobulin (BLG)-sensitized mice. The L9 supplement suppressed the aberrant balance of Th1/Th2 responses to BLG in mice, via upregulation of the CD4+CD25+Foxp3+Treg cell responses. The amount of CD4+CD25+Foxp3+Treg cells in mesenteric lymph nodes increased by 51.85%. Furthermore, administration of L9 significantly induced the expression of CD103 and reduced the maturation status of DCs in mesenteric lymph nodes, Peyer's patches, and spleen. Bone marrow-derived dendritic cells (BM-DCs) were activated by L9 in vitro, with an approximate 1.31-fold and 19.57-fold increase in expression of CD103 in CD11c+DCs and the level of IL-10 production, respectively, while the expression of CD86 did not change significantly. These data demonstrate that L9 reduced the BLG allergic sensitization, likely through regDCs mediated active suppression.

Maternal Perception of the Newborn and Confidence and Gratification of Mothering Role (어머니의 신생아 지각, 역할 수행에 대한 자신감 및 만족감)

  • 구현영;문영임
    • Journal of Korean Academy of Nursing
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    • v.28 no.4
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    • pp.920-930
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    • 1998
  • The purpose of this study was to evaluate maternal perception of the newborn, confidence and gratification of mothering role, and contribute to maternal nursing and neonatal nursing. The questionnaire, maternal perception of the newborn(Ⅰ) was completed by 47 mothers at three hospitals in the Kyoung-In area during the first to third day after birth. The questionnaire, maternal perception of the newborn(Ⅱ), confidence and gratification of mothering role were completed at fourth to sixth week after birth. The data were analyzed by a SPSS program. The results were as follows ; 1) The means of perception of the newborn(Ⅰ) and (Ⅱ) were 2.28±4.81 and 3.83±4.84. The means of confidence and gratification of mothering role were 40.38±5.51 and 39.30±6.70. 2) Half of the mothers(48.9%) rated their newborn as better than average newborn at first to third day after birth, and 74.5% of mothers did at fourth to sixth week after birth. 3) The score of maternal perception of the newborn (Ⅰ) was significantly correlated with the score of confidence and gratification of mothering role. The score of maternal perception of the newborn(Ⅱ) was correlated with the score of confidence and gratification of mothering role. The score of confidence and gratification of mothering role was related. 4) Maternal gratification of mothering role was significantly different by maternal perception of the newborn(Ⅰ), and maternal confidence was different by maternal perception of the newborn (Ⅱ). 5) Mothers who had religions and who wanted the pregnancy had higher perception of the newborn(Ⅰ) than those who did not. Mothers whose newborns were boys had higher perception of the newborn(Ⅱ) than those whose newborns were girls. Mothers who wanted the pregnancy had higher confidence of mothering role than those who did not. Mothers who planed cow's milk had lower gratification of mothering role than those who did not. The above findings indicate that mothers who have a different level of perception of the newborn, confidence and gratification of mothering role. Therefore, it is important that maternal perception of the newborn, confidence and gratification of mothering role were precisely evaluated, and nursing intervention for improving perception of the newborn should be provided for mothers.

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Physicochemical and Sensorial Properties of Probiotic Yogurt as Affected by Additions of Different Types of Hydrocolloid

  • Bahrami, Masoud;Ahmadi, Dariush;Alizadeh, Mohammad;Hosseini, Fakhrisadat
    • Food Science of Animal Resources
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    • v.33 no.3
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    • pp.363-368
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    • 2013
  • The main attributes of yogurt that affect consumer satisfaction are taste, consistency, and a firm texture. This study evaluates the influence of xanthan gum, barley beta-glucan, and guar gum in concentrations of 0.05%, 0.1%, 0.2%, and 0.3% on probiotic yogurt. The set-type yogurt samples were prepared by using raw cow's milk. The statistical analysis showed that none of these gum additions had any marked effect on pH, titratable acidity, total solids content, and probiotic bacteria counts of yogurt samples. Evaluations for syneresis and water-holding capacity (WHC) in the yogurt samples were affected by the type and concentration of the stabilizer. Yogurts treated with 0.1% xanthan gum and 0.3% beta-glucan recorded the highest WHC and the least syneresis. The largest amount of gel firmness was recorded in yogurt samples treated with 0.2% xanthan gum and 0.3% beta-glucan. Yogurt samples treated with 0.1% xanthan gum and 0.3% beta-glucan were considered acceptable by trained panelists and gained the highest scores in sensory evaluations. The correlation coefficient between the amount of syneresis, WHC and stiffness of texture was significant compared to scores for sensory evaluation (p<0.01). Results for effects of guar gum on the tested parameters were contrary to the results expected from a gum. According to this study, the use of xanthan gum and beta-glucan are highly recommended for low-fat yogurt production.

Attitudes and preferences of consumers toward food allergy labeling practices by diagnosis of food allergies

  • Ju, Se-young;Park, Jong-Hwan;Kwak, Tong-Kyoung;Kim, Kyu-earn
    • Nutrition Research and Practice
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    • v.9 no.5
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    • pp.517-522
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    • 2015
  • BACKGROUND/OBJECTIVES: The objective of this study was to investigate food allergens and prevalence rates of food allergies, followed by comparison of consumer attitudes and preferences regarding food allergy labeling by diagnosis of food allergies. SUBJECTS/METHODS: A total of 543 individuals living in Seoul and Gyeonggi area participated in the survey from October 15 to 22 in 2013. RESULTS: The results show that the prevalence of doctor-diagnosed food allergies was 17.5%, whereas 6.4% of respondents self-reported food allergies. The most common allergens of doctor-diagnosed and self-reported food allergy respondents were peaches (30.3%) and eggs (33.3%), respectively, followed by peanuts, cow's milk, and crab. Regarding consumer attitudes toward food labeling, checking food allergens as an item was only significantly different between allergic and non-allergic respondents among all five items (P < 0.001). All respondents reported that all six items (bold font, font color, box frame, warning statement, front label, and addition of potential allergens) were necessary for an improved food allergen labeling system. PLSR analysis determined that the doctor-diagnosed group and checking of food allergens were positively correlated, whereas the non-allergy group was more concerned with checking product brands. CONCLUSIONS: An effective food labeling system is very important for health protection of allergic consumers. Additionally, government agencies must develop policies regarding prevalence of food allergies in Korea. Based on this information, the food industry and government agencies should provide clear and accurate food labeling practices for consumers.

Intramammary preparation of enrofloxacin hydrochloride-dihydrate for bovine mastitis (biofilm-forming Staphylococcus aureus)

  • Diana Cordova-Gonzalez;Edgar Alfonseca-Silva;Lilia Gutierrez;Graciela Tapia-Perez;Hector Sumano
    • Journal of Veterinary Science
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    • v.25 no.1
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    • pp.6.1-6.11
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    • 2024
  • Background: Chronic bovine mastitis is linked to biofilm-producing Staphylococcus aureus (bp-Sa) or Staphylococcus coagulase-negative (bp-Scn). Objectives: Bp-Sa and bp-Scn were treated with intramammary preparations of either enrofloxacin HCl·2H2O-dimethyl-sulfoxide-chitosan (enro-C/DMSO/chitosan) or enro-C alone. Their potential to inhibit and degrade biofilm formation in vitro was also assessed. Methods: Milk samples were obtained from the affected quarters in a herd. Phenotypical and genotypical identifications as biofilm-producing Staphylococcus species were carried out. Enro-C/DMSO/chitosan and enro-C alone were assessed to determine their in vitro efficacy in interfering with biofilm formation and their bactericidal effects. A prolonged eight-day treatment with a twice-daily intramammary insertion of 10 mL of enro-C/DMSO/chitosan or enro-C alone was set to evaluate the clinical and bacteriological cures on day 10 in 15 cows per group and the biofilm-inhibiting ability. Results: Fifty-seven percent of the isolates were identified as Staphylococcus spp., of which 50% were bp-Sa, 46% bp-Scn, and 4% Staphylococcus pseudintermedius. One hundred percent of the S. aureus isolated and 77% of Staphylococcus coagulase-negative were biofilm producers. In both groups, the icaA and icaD biofilm-producing genes were identified. The experimental preparation could inhibit biofilm formation, degrade mature biofilms, and have well-defined microbicidal effects on planktonic and biofilm bacteria. The respective clinical and bacteriological cure rates were 100% and 80% for enro-C/DMSO/chitosan and 41.7% and 25% for enro-C alone. Conclusions: Enro-C/DMSO/chitosan eliminates bp-Sa and bp-Scn from cases of chronic bovine mastitis.