• Journal of Pharmaceutical Investigation
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• v.27 no.1
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• pp.1-10
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• 1997

For the purpose of improving the chemotherapeutic index of acyclovir(ACV), it was conjugated with asialofetuin(AF), which has been reported to enter into hepatocytes. When $[H^3]$ acyclovir in itself or its conjugate were administered to rats, the latter was taken up more selectively by the liver than any other tissues. The stability of ACVMP-AF conjugate in phosphate buffer (pH 5.0) and rat liver homogenate showed a pseudo-first order profile. ACVMP-AF, however, was relatively stable in pH7.4 phosphate buffer and rat plasma. The conjugate was added to the isolated rat hepatocyte and cellular uptake was monitored by scintillation counting for up to 6 hours at $37^{\circ}C$. Hepatocytes incubated with the conjugate exhibited radioactivities significantly enhanced over control levels dose-dependently, i.e., a 3-40 fold increase in radioactivities was observed over controls at the conjugate concentrations of $0.1-10\;{\mu}g/ml$. The AUQ in the liver, kidney, spleen, intestine and lung was higher in treatment with ACVMP-AF than that in treatment with ACV. In treatment with ACVMP-AF, the weighted-average overall drug targeting efficiency(Te) for the liver was higher than in treatment with ACV(57.00 vs 13.31 %), and the weighted-average tissue exposure(Re) was 5.03 for the liver. These results indicated that ACVMP-AF conjugate was rapidly taken up by hepatocytes and could be an efficient and selective hepatic targeting system.

• Korean Journal of Animal Reproduction
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• v.20 no.2
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• pp.163-170
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• 1996

The effect of several potential antioxidants and amino acids were examined as a means of increasing the development of in vitro matured and in vitro fertilized oocytes into morulae or blastocysts. Bovine embryos developed to the 2~8 cell stage after in vitro fertilization were cultured for 5 to 6 days at 39$^{\circ}C$ in CR1aa containing varing concentraton of the antioxidants and amino acid in a gas phase consisting of 5% CO2, high humidified air. At 5~6 days, embryo developments were reduced, and embryos were fixed and stained with Hochest 33342 DNA stain to facilitate counting of cells. In experiment 1, the proportion of embryos developed to morulae and blastocysts in CR1aa containing 1mM, 2.5mM taurine (22.6% and 20.4%) was slightly higher than those of 0, 5 and 10mM Taurine (5.7, 5.7 and 3.9%, P<0.05). In experiment 2, addition of glutathione did not improve blastocyst development (P>0.05). In experiment 3, concentations of superoxide dismutase(SOD) ranging from 300 to 1,000 U did not affect the propotion of embryos developing into blastocysts (P>0.05). In experiment 4, addition of 250 U catalase(38.5%) was slighty higher than those of 0, 500 and 1,000U. In experiment 5, the proportion of embryo developed beyond morula stage in CR1aa with taurine plus EDTA was slighty higher than other treatments(15.7, 26.0 and 29.2%), there were no significantly increases in cell number among treatments(P>0.05). These results are indicating that antioxidants and amino acids can increase the proportion of embryos that develop into morulae and blastocysts, but did not increas in cell number of blastocysts.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.21 no.1
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• pp.12-17
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• 2008

In this paper, an I-V characteristics of bypass diode has been studied by counting the shading effect in photovoltaic module. The shadow induces hot spot phenomenon in PV module due to the increase of resistance in the current path. Two different types of PV module with and without bypass diode were fabricated to expect maximum output power with an increasing shading rate of 5 % on the solar cell. Temperature distribution is also detected by shading the whole solar cell for the outdoor test. From the result, the bypass diode works properly over 60 % of shading per cell with constant output power. Maximum power generation in case of solar cell being totally shaded with bypass diode decreases 41.3 % compared with the one under STC(Standard Test Condition). On the other hand, the maximum output power of the module without bypass diode gradually decreases by showing hot spot phenomenon with the increase of shading ratio on the cell and finally indicates 95.5 % of power loss compared with the output under STC. Finally the module temperature measured increases around $10^{\circ}C$ higher than that under STC due to hot-spots which come from the condition without bypass diode. It has been therefore one of the main reasons for degrading the PV module and shortening the durability of the PV system.

• Korean Journal of Optics and Photonics
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• v.7 no.4
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• pp.341-347
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• 1996

The photocount distribution from a quasi-thermal light source, a moving ground glass disk (surface roughness; 9 ${\mu}{\textrm}{m}$) illuminated by a well-stabilized He-Ne laser, is measured by a photon counting system, and analyzed with theoretical calculations. The distribution approaches the Poisson distribution for the long coherence time ${\tau}_c$ compared to the measuring time T. The coherence time ${\tau}_c$ of the quasi-thermal source can be changed by controlling the velocity v of the motor driving the glass disk. By the comparison of experimental results and theory for the condition of T/ ${\tau}_c$ >>1, the coherence time ${\tau}_c$ of the quasi-thermal source is turned out to be in the range of 31.43 $mutextrm{s}$~2.48 $mutextrm{s}$ according to the circumferential velocity of the disk, and compared with the simple calculation of $\sigma$/v.

• Journal of Drive and Control
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• v.16 no.4
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• pp.23-31
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• 2019

The purpose of this study was to evaluate the PTO severeness for an agricultural tractor during disk plow and rotary tillage. The PTO load measurement system was constructed with data acquisition and a PTO torquemeter. Field experiments were conducted at a combination of traveling speed (L3 Low, L3 High) and PTO speed (P1, P2). The load spectrum was generated using the rain-flow counting method, and the SWT method was used to consider the range and mean of the PTO load. The damage sum was calculated by applying a modified miner rule, which is a cumulative damage law. The relative severeness was expressed as the ratio of the lowest damage sum. Relative severeness was higher with the lower PTO gear stage, and higher driving gear stage and it was approximately 40-102 times higher for rotary tillage than disk plow tillage in the same gear stages. The relative severeness was 1010.12 in the rotary tillage under L3 High P1 based on the disk plow tillage under L3 Low P2.

• Journal of IKEEE
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• v.23 no.4
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• pp.1328-1336
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• 2019

To maintain the safe and optimal performance of batteries, accurate estimation of state of charge (SOC) is critical. In this paper, Long short-term memory network (LSTM) based on the artificial intelligence algorithm is applied to address the problem of the conventional coulomb-counting method. Different discharge cycles are concatenated to form the dataset for training and verification. In oder to improve the quality of input data for learning, preprocessing was performed. In addition, we compared learning ability and SOC estimation performance according to the structure of LSTM model and hyperparameter setup. The trained model was verified with a UDDS profile and achieved estimated accuracy of RMSE 0.82% and MAX 2.54%.

• Biomedical Science Letters
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• v.24 no.1
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• pp.30-34
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• 2018

Silver (Ag) has been widely used in commercial products and medical fields since ancient times because of its antibacterial effect. It is harmless and non-toxic to the human body. For this reason, recent research has actively evaluated antimicrobial activity using silver (Ag). In this study, we investigated the inhibitory effect of a silver-based compound, silver phosphate ($Ag_3PO_4$) on the growth of Staphylococcus aureus and the activation of human immunity. First, the inhibitory effect of $Ag_3PO_4$ on the growth of Staphylococcus aureus was confirmed by a growth curve and a colonyounting method. As a result, the growth inhibitory effect increased as the concentration of $Ag_3PO_4$ increased. Specifically, treatment with $5{\mu}g/mL$ of $Ag_3PO_4$ resulted in no bacteria growth, and the colony-counting method showed a remarkable inhibition. In addition, the expression of cytokine IL-8 by $Ag_3PO_4$ was examined to investigate the cellular immune system activation by $Ag_3PO_4$. After pretreatment of Staphylococcus aureus for 1 hour with $50{\mu}g/mL$ $Ag_3PO_4$, an increased IL-8 mRNA expression resulted. In cells treated with $Ag_3PO_4$, we found that the expression of IL-8 was enhanced in a time-dependent fashion compared to non-treated cells. These results indicate that $Ag_3PO_4$ induces antimicrobial activity against Staphylococcus aureus and activates human immunity. These results are expected to contribute to the future study of the mechanism of silver (Ag) and silver-based compounds in relation to antibacterial activity.

• Journal of the Korean Society for Precision Engineering
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• v.24 no.7 s.196
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• pp.120-125
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• 2007

Analysis of elders' behavioral characteristic during showering provides important fundamental data when developing an automatic showering system. We recorded showering behavior of 37 elders' at the front, back, left and right view using four CCTV cameras. The photographic data were analyzed by counting the frequencies of body scrub and identifying zones where hands could reach. And we measured their body sizes, and conducted questionnaire of showering for 302 peoples. Analysis was done according to the analysis criteria established. The human body was divided into 14 zones from a body, and the numbers of body scrub for each zone were counted. The average total numbers of body scrub were 170.8 excluding the head, the face and the neck. The average numbers of body scrub with right hand were 70.2, left hand were 88.2, and both hands were 81.8. The percentages of body scrub frequencies were 16.3% (breast), 12.2%(crotch), 8.8% (abdomen), 7.5% (waist), 8.8% (shoulder), 7% (hips), 0.4% (back), 15.2% (arm), and 23.9% (leg). Elders scrubbed the breast, crotch and abdomen more frequently than the back and hips. According to the video analysis and questionnaire results, the distribution of shower heads (or nozzles) at the front side should be arranged at the ratio of 40% of the trunk(breast, shoulders, abdomen), 20% of the thighs, the calves, and the crotch, respectively. The distribution of shower heads (or nozzles) of the back side were arranged at the ratio of 20% of the waist and the arms, 20% of the back, the thighs, the calves, and the hip, respectively.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.4
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• pp.1851-1857
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• 2014

Objective: The main purpose of this work was to investigate the effect of berberine hydrochloride (BH) on the proliferation, apoptosis, migration, and invasion of CNE-1 nasopharyngeal carcinoma cells. Our results shed light on the functional components of traditional Chinese herbs for potential use in modern medicine. Methods: The CNE-1 cell line was treated with different concentrations of BH and effects on cell viability and proliferation were evaluated using the Cell Counting Kit-8 (CCK-8) assay. Anti-migratory and anti-invasive actions of BH were investigated using wound healing assays and the Millicell Hanging cell culture insert system, respectively. Expression of the epithelial-mesenchymal transition (EMT)-related gene twist (Twist) was analyzed by real-time PCR and Western blotting. Apoptosis was estimated with an annexin-V fluorescein (FITC) apoptosis detection kit, as well as with reference to levels of activated caspase-3 of CNE-1 cells before and after treatment with BH utilizing fluorescence spectroscopy. Results: BH was capable of reducing proliferation and viability of CNE-1 cells in a dose- and time-dependent manner, also demonstrating anti-migratory and anti-invasive capacities which correlated with reduction in expression of Twist. Finally, BH was able to induce significant amounts of apoptosis in CNE-1 cells, as demonstrated by an increase in the activity of caspase-3 and in annexin-V staining following treatment. Conclusion: BH extracted from rhizoma coptidis demonstrated an ability to block proliferation, induce apoptosis, and impair the migration and invasion of the CNE-1 cell line Considering these properties, our results suggest that BH could be an important compound for consideration in the treatment of nasopharyngeal carcinoma.

• The Journal of the Acoustical Society of Korea
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• v.13 no.2E
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• pp.26-31
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• 1994

A new feature extraction technique utilizing strange attractor and artificial neural network for speaker recognition is presented. Since many signals change their characteristics over long periods of time, simple time-domain processing techniques should e capable of providing useful information of signal features. In many cases, normal time series can be viewed as a dynamical system with a low-dimensional attractor that can be reconstructed from the time series using time delay. The reconstruction of strange attractor is described. In the technique, the raw signal will be reproduced into a geometric three dimensional attractor. Classification decision for speaker recognition is based upon the processing or sets of feature vectors that are derived from the attractor. Three different methods for feature extraction will be discussed. The methods include box-counting dimension, natural measure with regular hexahedron and plank-type box. An artificial neural network is designed for training the feature data generated by the method. The recognition rates are about 82%-96% depending on the extraction method.