• Title/Summary/Keyword: Coulter Counter

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Comparison of Size Distributions of Rice Flour Measured by Microscope, Sieve, Coulter counter, and Aerodynamic Methods (현미경법, 표준체법, 전기저항법, 공기역학법에 의한 쌀가루의 입도분포의 비교)

  • Kim, Young-Jin;Kim, Sang-Sook
    • Korean Journal of Food Science and Technology
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    • v.26 no.2
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    • pp.184-187
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    • 1994
  • Size distributions of rice flour measured by microscope, sieve, Coulter counter (electrozone) and aerodynamic (Aerosizer) methods were compared. Among the particle sizes measured by four methods, those by microscope and sieve were the smallest and the largest, respectively. Difference was found between the particle sizes measured by Coulter counter and aerodynamic methods.

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Particle Size Distribution Analysis of Mineral Dust in Polar Snow Using a Coulter Counter (쿨터카운터(Coulter Counter)를 이용한 극지 눈시료 중 광물성 먼지의 입자크기분포 분석)

  • Kang, Jung-Ho;Hwang, Heejin;Hong, Sang Bum;Hur, Soon Do
    • Ocean and Polar Research
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    • v.36 no.4
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    • pp.319-326
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    • 2014
  • Mineral dust in the polar snow plays an important role both in the climate system of the Earth and in global biogeochemical cycles. Analysis of the concentration and the particle size distribution of mineral dust has been carried out in the snow from the Antarctic surface snow and the Greenland snowpit. Among the various particle size determination techniques, a Multisizer 3 Coulter Counter in a class 100 clean bench counted all particles between 1.1 and $30.0{\mu}m$ with a $50{\mu}m$ aperture tube. The aperture tube size, the concentration of electrolytes and the accuracy of the particle size distribution were determined in this study. The number concentrations from the Antarctic surface snow were 81,843 particles $mL^{-1}$, but those from the Greenland snowpit were 10,666 particles $mL^{-1}$. In the volume distribution, the distributions of mineral dust in both the Antarctic surface snow and the Greenland snowpit showed lognormal distribution in the size interval 1.1 to $6.0{\mu}m$ with the mode, 3.562 and $3.836{\mu}m$, respectively. The analysis technique using a coulter counter for mineral dust could be used for reconstructing paleoclimates from polar ice cores.

Comparison of Fossomatic and Coulter Counter Methods for Somatic Cell Count in Raw Milk (원유내 체세포수 측정을 위한 Fossomatic과 Coulter Counter 방법의 비교)

  • 이정구;손봉환;이정길;고홍범
    • Korean Journal of Veterinary Service
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    • v.16 no.1
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    • pp.1-10
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    • 1993
  • Samples of bulk herd milk, foremilk, last milk (stripping) and individual cow sample were collected and their somatic cell number were counted with Fossomatic counter (FCC), Coulter counter(CC), direct microscopic somatic cell count(DMSCC) and Califormia mastitis test (CMT), The results were compared and summarized as follows : 1. Mean somatic cell counts of 120 bulk herd milk samples obtained by DMSCC, FCC and CC were 433,203, 481,213 and 676,245 respectively. 2. Mean somatic cell counts of 116 foremilk samples obtained by DMSCC, FCC and CC were 515,035, 611,845 and 725,051 respectively 3. Mean somatic cell counts of 87 last milk samples obtained by DMSCC, FCC and CC were 718,506, 839,874 and 1,041,160 respectively. 4. Mean somatic cell counts of 57 individual cow samples obtained by DMSCC, FCC and CC were 449,258, 491,018 and 521,315 respectively. 5. Mean somatic cell counts of all samples increased with the increasing CMT score, and the cell counts were higher by CC than by FCC. 6. The correlation coefficients between the somatic cell counts by CMT and CC were 0.926 in bulk herd milk, 0.707 in foremilk 0.688 in last milk and 0.675 in individual cow sample, respectively 7. The correlation coefficients between the somatic cell counts by CMT and FCC were 0. 945 in bulk herd milk, 0.705 in foremilk 0.694 in last milk and 0.727 in individual cow sample, respectively. 8. The correlation coefficients between the somatic cell counts by CC and FCC were 0.978 in bulk herd milk, 0.997 in foremilk 0.983 in last milk and 0.985 in individual cow sample, respectively.

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Micro Cell Counter Using a Fixed Control Volume Between Double Electrical Sensing Zones (다수의 계수구역간의 검사체적을 이용한 소형 세포농도센서)

  • Lee Dong Woo;Yi Soyeon;Cho Young-Ho
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.29 no.12 s.243
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    • pp.1615-1620
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    • 2005
  • We present a novel flow-rate independent cell counter using a fixed control volume between double electrical sensing zones. The previous device based on the single electrical cell sensing in a given flow-rate requires an accurate fluid volume measurement or precision flow rate control. The present cell counter, however, offers the flow-rate independent method for the cell concentration measurement with counting cells in a fixed control volume of $22.9{\pm}0.98{\mu}{\ell}$. In the experimental study, using the RBC (Red Blood Cell), we have compared the measured RBC concentrations from the fabricated devices with those from Hemacytometer. The previous and present devices show the maximum errors of $20.3\%\;and\;16.1\%$, which are in the measurement error range of Hemacytometer (about $20\%$). The present device also shows the flow-rate independent performance at the constant flow-rates ($5{\mu}{\ell}/min$ and $10{\mu}{\ell}/min$) and the varying flow-rate (4, 2, and $4{\mu}{\ell}/min$). Therefore, we demonstrate that the present cell counter is a simple and automated method for the cell concentration measurement without requiring an accurate fluid measurement and precision flow-rate control.

Fabrication of electrodes on mcirochannel side wall using shadow evaporation effect (샤도우 증착 효과를 이용한 마이크로 채널내 측벽 전극 제작)

  • 강길환;김규만
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2004.10a
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    • pp.1462-1465
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    • 2004
  • A new method to fabricate metal electrodes on side wall of the microchannel is presented. Coulter counter allows to count the number of cell passing through the microchannel by detecting impedance variation between two electrodes. The relative position of two electrodes is important for sensitivity of impedance measurement. 100nm thick Al electrodes are deposited on the channel side wall by means of shadow evaporation.

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실험동물 마우스의 혈액세포 분포에 관한 연구

  • Ian C. Guest
    • Toxicological Research
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    • v.7 no.1
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    • pp.47-50
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    • 1991
  • Hematological studies in two strains of normal laboratory mice, namely ICR and ddY which were produced in Koren were carried out. Blood analysis was performed by using different methods, a fully automated analyzer (ELT 1, 500), Coulter counter (model S plus IV) and manual technique to examine possible differences resulting from methodolgies'slight differences among the values of blood parameters were obtained. There was a slight difference noted between sexes, however, similar values were obtained from both strains.

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A study on analysis of particle size distribution

  • Min, Shin-Hong
    • Archives of Pharmacal Research
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    • v.3 no.2
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    • pp.65-74
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    • 1980
  • Analysis of particle size distribution of a sample of fine aluminum hydroxide powder was carried out by four different methods, i. e., conductivity, air permeability, gas-adsorption and sedimentation. Each method was reproducible. The results obtained by Coulter counter and sedimentation balance were similar, and the data obtained by Lea and Nurse permeameter and Stroehlein areameter were also similar. But the results differ considerabley between the former and the latter. The advantages and disavantages of each method were discussed briefly and a means of comparing the results with those obtianed by surface area measurements was shown.

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Synchronization of Cell Cycle in Korean Hydrogen Producing Cyanobacterial Strains (한국산 수소생산 남세균 종주들의 세포주기 동조화)

  • Park, Jong-Woo;Ahn, Se-Hee;Kim, Hyung-Seop;Yih, Won-Ho
    • Transactions of the Korean hydrogen and new energy society
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    • v.22 no.5
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    • pp.663-670
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    • 2011
  • Under a daily photoperiod of 14h light and 10h dark synchronization of cell cycle in Korean Cyanothece spp. strains and $Synechococcus$ sp. strain Miami BG043511 was analyzed as to be applicable to enhanced hydrogen production. For all strains peaks of double cell were observed during the light period of a daily cycle. Peaks of maximal cell size measured by a coulter counter appeared at the peak of double cells observed under light microscope reconfirming the synchronization of daily cell cycle. The cell cycle synchronization became weakened within two days when treated with continuous illumination. Rapid detection of the peak time of double cell percentage by coulter counters may contribute to quasi-realtime feedback control for efficient production of photobiological hydrogen by unicellular cyanobacterial strains.

Process Evaluation in Preparing Oil in Water Microemulsion by Dynamic Light Scattering Method (Dynamic Light Scattering Method를 이용한 수중유형 Microemulsion의 제조조건 평가)

  • Min, Shin-Hong;Yang, Joong-Ik;Kwon, Jong-Won;Jeong, Dae-Sik;Jeong, Yeoub
    • Journal of Pharmaceutical Investigation
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    • v.17 no.1
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    • pp.41-46
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    • 1987
  • Oil in water microemulsion containing soybean oil and egg phosphatide was prepared by vacuum high shear mixing and high pressure homogenizing. The laser particle sizer, Coulter counter and photomicroscope were used to determine the particle size distribution at each cycle of homogenizing. Particularly, the laser particle sizer(dynamic light scattering method) was applied to the study of particle size distribution behavior below $1\;{\mu}m$. It was found that the particle size distribution below $1\;{\mu}m$ was shifted to lower size range as the number of passing cycle was increased. Beyond the 7th cycle, however, the particle size distribution was not varied.

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Physicochemical Evaluation of Oil in Water Microemulsions (수중유형 마이크로에멀젼의 물리화학적 평가)

  • Chung, Myung-Hwa;Jheong, Yeoub;Jheong, Dae-Sik;Kwon, Jong-Won;Yang, Joong-Ik;Min, Shin-Hong
    • Journal of Pharmaceutical Investigation
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    • v.18 no.1
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    • pp.9-13
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    • 1988
  • Physicochemical properties of oil in water microemulsions containing soybean oil and egg phosphatide were observed for 3 weeks under the storage condition of $4^{\circ}C$ refrigerator. Changes in major fatty acid content, particle size distribution, rheogram, acid value and pH value were measured by gas chromatograph, laser particle sizer, Coulter counter and rheometer. From above experiments following conclusions were obtained; 1) Mean particle diameter was shifted from 240 to 266mm. 2) No significant changes were observed in the content of major fatty acids of soybean oil, rheogram, acid value and pH value.

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