• 한국약용작물학회지
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• 제27권3호
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• pp.194-201
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• 2019

Background: The Ministry of Environment, Korea recognizes Cotoneaster wilsonii Nakai as a second-class endangered plant. It is a native species that grows in Ulleung-do, Korea. To our knowledge, the bioactivity of this plant has not yet been reported. Therefore, in this study, we have reported the bioactivity of C. wilsonii Nakai. Methods and Results: The anti-oxidant activities of C. wilsonii methanolic extract were investigated in vitro. The anti-oxidant activity was evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay, and the total phenolic and flavonoid content were measured. The leaf methanolic extract had the highest DPPH radical scavenging activity ($IC_{50}$; $15.74{\mu}g/m{\ell}$), and it also had the highest total phenolic and flavonoid content ($220.95mg{\cdot}GAE/g$, and $36.46mg{\cdot}QE/g$ extract respectively). Through high performance liquid chromatography (HPLC) analysis, chlorogenic acid was found to be the predominant among all phenolic compounds, showing a concentration of 84.24 mg/g extract. More than 60% decrease in nitric oxide (NO) production was found in the leaf methanolic extract. Conclusions: To the best of our knowldege, this is the first report of the bioactivities of C. wilsonii. The results demonstrate that the leaf methanolic extract of C. wilsonii shows potent anti-oxidant, and anti-inflammatory activities.

• 한국약용작물학회지
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• 제27권6호
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• pp.412-418
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• 2019

Background: Previously, studies have observed that the leaf extract of Cotoneaster wilsonii Nakai has potent antioxidant and anti-inflammatory activites. Therefore, further research was conducted to separate the active antioxidant and anti-inflammatory compounds in the leaf of the C. wilsonii. Methods and Results: The anti-oxidant effects were evaluated by analyzing the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, estimating the totla phenolic content (TPC) and total flavonoid content (TFC), and using reducing power assay method. The ethyl acetate (EtOAc) fraction demonstrated the highest DPPH radical scavenging activity (IC₅₀; 9.69 ㎍/㎖), and the highest TPC and TFC (345.98 mg·GAE/g, and 74.23 mg·QE/g). Moreover, it indicated the highest effects on nitric oxide production. Therefore, the active compound was separated using the EtOAc fraction, determined on the basis of spectral data, and identified as (+)-catechin. Conclusions: The first compound separated was made from the leaf of C. wilsonii was (+)-catechin, which produced potent anti-oxidant and anti-inflammatory effects.

• 한국환경생태학회지
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• 제17권2호
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• pp.133-143
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• 2003

본 연구 결과 울릉도에 자생하는 섬개야광나무의 자생지를 현재 천연기념물로 지정되어 있는 곳 외에 추가로 탐문조사와 현지조사를 통하여 새로운 자생지 두 군데를 찾아내었다. 현지 조사결과 남양 일몰대는 자생지가 일몰대 부근에 위치하여 사람에 의한 체손이 우려되며, 통구미의 자생지는 자생지의 위치가 산 능선부에 위치하기 때문에 사람의 접근이 불가능하고 치수의 생육이 좋은 것으로 밝혀졌다. 각 자생지에 생육하고 있는 개체는 약 25개체에서 50개체이며, 앞으로 기존의 천연기념물지역을 복원하기 위하여 유용한 정보를 제공할 것으로 판단된다. 섬개야광나무의 형태적 분석결과 꽃의 경우 지역별로 화판장과 화판폭의 차이가 없었지만, 잎의 경우에는 엽장. 엽폭. 엽면적 모두 3개지역에서 차이를 나타내었다 이는 섬개야광나무가 다른 식물과 달리 서식처가 분획화되어 생육하는 지역의 환경적 특징에 의해서 잎의 모습이 차이가 나는 것으로 판단된다.