• KSBB Journal
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• 제27권3호
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• pp.151-156
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• 2012

Lactic acid is an important product arising from the anaerobic fermentation by lactic acid bacteria (LAB). It is used in the pharmaceutical, cosmetic, chemical, and food industries as well as for biodegradable polymer and green solvent production. The poly lactic acid (PLA) is an important material for bio-plastic manufacturing process. For PLA production by new LAB, we screened LAB isolates from shellfish. A total of 28 LAB were isolated from various shellfishes. They were all Gram positive, oxidase and catalase negative. Based on API 50CHL kit, 7 strains among the 28 isolates were identified as Lactobacillus plantarum, 6 strains as Lactobacillus delbrueckii, 5 strains as Leuconostoc mesenteroides, 3 strains as Lactobacillus brevis, 2 strains as Lactococcus lactis, 1 strain as Lactobacillus salivarius, 1 strain as Lactobacillus paracasei, 1 strain as Lactobacillus pentosus, 1 strain as Lactobacillus fermentum and 1 strain as Pediococcus pentosaceu. Also, we examined the amount of total lactic acid produced by these new strains by HPLC analysis with Chiralpak MA column. One strain E-3 from Mytilus edulis was indentified as Lactobacillus plantarum and found to produce 20.0 g/L of D-form lactic acid from 20 g/L of dextrose. Further studies are underway to increase the D-lactic acid production by E-3.

• KSBB Journal
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• 제24권1호
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• pp.25-29
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• 2009

Saccharomyces cerevisiae의 deletion mutant를 이용하여 ydc1, ypc1, scs7, sur1, csg2, ipt1, Icb4, Icb5, dpll의 deletion이 세라마이드의 생산에 미치는 영향을 고찰하였다. 세라마이드는 ELSD가 연결된 HPLC를 통하여 분석하였으며 ${\triangle}$ydc1 mutant의 세라마이드 생산량이 6 mg ceramide/g cell로 최대량을 나타내었으며 ${\triangle}$sur1 mutant, ${\triangle}$lcb5 mutant, ${\triangle}$dpll mutant의 경우 control로 사용한 BY4742와 비슷한 세라마이드 생산량을 나타내었고, 그 외 ${\triangle}$ypc1 mutant, ${\triangle}$scs7 mutant, ${\triangle}$csg2 mutant, ${\triangle}$ipt1 mutant, ${\triangle}$lcb4 mutant는 BY4742보다 낮은 세라마이드 생산량을 나타내었다.

• 농업과학연구
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• 제43권2호
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• pp.249-257
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• 2016

In this study, rapeseed extracts were obtained by supercritical carbon dioxide fluid extraction of defatted rapeseed to evaluate the stability and antioxidant activity of an oil-in-water (O/W) emulsion system. The oil-in-water emulsions were prepared from stripped soybean oil with different concentrations (0.3, 0.4, 0.5, and 0.6%) of rapeseed extract as an emulsifier. Their emulsion stability was compared to that of emulsions prepared with the commercial emulsifier, Tween 20 (Polysorbate 20, 0.2%). After stripping the soybean oil, the total tocopherol content was reduced from 51.4 g/100 g to 1.1 g/100 g. Emulsion stability and oxidative stability of emulsions prepared with Tween 20 and rapeseed extract as emulsifiers were evaluated. For 30 days droplet sizes of emulsions containing rapeseed extract (0.4, 0.5, and 0.6%) were not significantly different (p > 0.05). Similar results were obtained for emulsion stability (ES) and Turbiscan analysis, suggesting that the addition of rapeseed extract increased emulsion stability. The addition of rapeseed extract at more than 0.4% resulted in an emulsion stability comparable to the addition of 0.2% Tween 20. The antioxidative ability of rapeseed extract increased with the amount added in the emulsion. Moreover, the addition of 0.6% rapeseed extract resulted in the lowest emulsion peroxide values (10.3 mEq/L) among all treatments. Therefore, according to the stability of its antioxidative and physical stability properties, rapeseed extract from super critical extraction could be successfully applied to the food and cosmetic industries.

• KSBB Journal
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• 제19권1호
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• pp.1-11
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• 2004

클로렐라는 생물화학적 성분들을 풍부하게 함유하고 있어서 영양학적으로 사료나 식품으로 이용되며 화장품, 의약품, 심지어 연료로도 이용되고 있다. 클로렐라는 보통 연못이나 호수 등 담수에서 생육하며, 구형 단세포이다. 생식은 무성생식으로 하루에 4-16배로 증식한다. 클로렐라의 대량생산을 위한 배양은 크게 옥외배양법과 발효조 배양법으로 나뉜다. 클로렐라는 필수 아미노산과 생체에 여러 가지 생리적 기능을 수행하는 지방산과 스테롤, 유산균 성장 촉진 원인 물질인 chlorella growth factor (CGF)의 함유되어 있으며, 생체 내에서 중금속 배출기능, 독성물질의 분해, 동맥경화 및 간장장애의 억제, 면역기능 강화, 항암 활성, 장내 유효세균의 증식 촉진, 식품의 풍미 향상 및 보습효과 등의 기능성을 가지고 있어 기능성 생물소재로 주목되고 있다. 클로렐라는 건강기능식품, 화장품, 의약품 소재로서 응용될 수 있을 뿐만 아니라 이산화탄소의 고정화와 수소가스의 생성 등 환경 및 에너지의 생산을 위한 수단으로서의 역할이 전망된다.

• Journal of Microbiology and Biotechnology
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• 제22권1호
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• pp.84-91
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• 2012

Two staphylococcal lipases were obtained from Staphylococcus epidermidis S2 and Staphylococcus aureus S11 isolated from sebaceous areas on the skin of the human face. The molecular mass of both enzymes was estimated to be 45 kDa by SDS-PAGE. S2 lipase displayed its highest activity in the hydrolysis of olive oil at $32^{\circ}C$ and pH 8, whereas S11 lipase showed optimal activity at $31^{\circ}C$ and pH 8.5. The S2 lipase showed the property of cold-adaptation, with activation energy of 6.52 kcal/mol. In contrast, S11 lipase's activation energy, at 21 kcal/mol, was more characteristic of mesophilic lipases. S2 lipase was stable up to $45^{\circ}C$ and within the pH range from 5 to 9, whereas S11 lipase was stable up to $50^{\circ}C$ and from pH 6 to 10. Both enzymes had high activity against tributyrin, waste soybean oil, and fish oil. Sequence analysis of the S2 lipase gene showed an open reading frame of 2,067 bp encoding a signal peptide (35 aa), a pro-peptide (267 aa), and a mature enzyme (386 aa); the S11 lipase gene, at 2,076 bp, also encoded a signal peptide (37 aa), pro-peptide (255 aa), and mature enzyme (399 aa). The two enzymes maintained amino acid sequence identity of 98-99% with other similar staphylococcal lipases. Their microbial origins and biochemical properties may make these staphylococcal lipases isolated from facial sebaceous skin suitable for use as catalysts in the cosmetic, medicinal, food, or detergent industries.

• 한국미생물·생명공학회지
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• 제39권3호
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• pp.189-199
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• 2011

최근에 해조류 유래 기능성소재는 항종양성, 항바이러스성, 항혈액응고 및 면역력 증강 등의 다양한 생리활성기능을 갖는 것으로 알려져 있다. 특히 해조다당류를 저분자화하면 다양한 생체조절기능성이 월등히 높게 나타나고 있음이 보고되고 있다. 따라서 해조다당류를 해조류로부터 효과적으로 추출할 수 있는 추출방법의 최적화 및 해조다당류의 저분자화를 통하여 해조다당류 기능성 증진 방법에 대한 연구가 활발히 진행되고 있다. 본 총설에서는 최근 부각되고 있는 해양 미생물 유래 해조다당류 분해효소를 이용한 기능성 신소재 개발 및 산업적 응용에 대하여 논하고자 한다.

• Journal of Microbiology and Biotechnology
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• 제23권2호
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• pp.237-245
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• 2013

Canthaxanthin (cx) is a potent antioxidant that is chemically synthesized at the industrial scale and has imperative applications in the cosmetic and feed industries. An orange pigmented mesophilic bacterium, designated as K44, was isolated from soil samples of Kargil, India. Biochemical tests, 16S rRNA gene sequencing, and FAME analysis of the bacterium indicated it to belong in the genus Dietzia and is distinct from human isolates. The strain showed 98% 16S rRNA gene sequence homology with Dietzia maris DSM 43102. High-performance liquid chromatography profile of the pigments isolated from K44 showed two major peaks absorbing at 465.3 and 475 nm. The liquid chromatography-mass spectrometry (LC-MS) analysis of both these peaks revealed their m/z to be 564. The molecular weights, LC-MS/MS fragmentation patterns, and ${\lambda}_{max}$ of these fractions corresponded to all-trans- (475 nm) and 9-cis-(465.3 nm) cx isomers. The antioxidant activities of cis- and trans-cx isomers isolated from this bacterium were found to differ, where the cis-isomer showed higher free radical, superoxide radical, and reactive oxygen species scavenging activities than the alltrans- isomer, suggesting that 9-cis-cx is more effective as an antioxidant than the all-trans-cx.

• 한국패션뷰티학회지
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• 제5권4호
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• pp.130-138
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• 2007

The aim of this study was to assess the cosmeceutical activies of four kinds of Korea herb medicine extracts using in cosmetics and related industries. The cosmeceutical activities of extracts were investigated by tyrosinase inhibition, astringent, anti-bacterial and MTT assay for cell viability. In the whitening effect, PA that the highest tyrosinase inhibition activity showed 56% at 10 ppm in ethanol extract. Also water and ethanol extract of RE showed 54%, 68% at 1,000 ppm, respectively, but LE and CA showed lower effect. Astringent effect of water and ethanol extract of PA appeared over 60% at 1,000ppm concentration but other extracts showed no astringent effect. In the anti-bacterial test, water and ethanol extract of PA showed no anti-bacterial effect against all microorganisms. But water and ethanol extract of RE showed anti-bacterial effect on Staphylococcus aureus, ethanol extract of showed on Staphylococcus aureus and Propionibacterium acnes, ethanol extract of CA showed on Candida albicans. The resUlt of stability test showed that the emulsion of containing PA were very stable at various temperature and sun-light test. Viscosity and pH of emulsion did not change. From the results of human patch test to assess the safety of cosmetics containing PA there was no negative reaction on skin was found.

• 융합정보논문지
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• 제11권12호
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• pp.229-235
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• 2021

K-Beauty 미용산업은 고부가가치 창출이 가능한 미래지향적 성장산업으로 부각되고 있다. 이에 본 연구는 소비자들의 미래가치적 니즈와 경쟁력을 갖춘 지속적인 유망 산업으로 발전하기 위한 방향성 제시를 목적으로 K-Beauty 미용산업의 동향, 최신 화장품 산업 트렌드, 글로벌 경쟁력 관련 선행연구와 보고서를 분석하였다. 먼저 혁신성과 창의성을 기반으로 산업과 정부의 부문별 역할 분담을 가능하게 하여 수출효자 산업으로의 사회적, 경제적 가치의 입증을 유도해야 하며, 두 번째는 정보통신기술을 기반으로 뷰티와 IT를 융합하여 혁신네트워크 형성과 확대를 도모해야 성공할 것으로 보여지며, 마지막으로 정부가 제시한 비전에 발맞추어 신규일자리 창출과 산업발전 목표에 맞는 전문인력 양성이라는 과제 해결을 위한 법적, 제도적인 개선이 선행된다면 미래지향적인 K-Beauty 미용산업 활성화를 전략화 할 수 있을 것이라 판단된다.

• Journal of Nutrition and Health
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• 제52권2호
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• pp.168-175
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• 2019

Purpose: Barely sprout is a well-known oriental herbal medicine with a wide range of health benefits. Recent studies have provided scientific evidence of its therapeutic effects with expanded application. This study investigated anti-melanogenic effect of barley sprout water extract (BSE) in murine melanocyte B16F10. Methods: Various concentrations (0, 50, 125, and $250{\mu}g/mL$) of BSE and arbutin (150 ppm) were applied to B16F10 stimulated with or without alpha-melanocyte stimulating hormone (100 nM) for 72 hours. The whitening potency of BSE was determined altered cellular melanin contents. Activity and expression of tyrosinase and microphthalmia-associated transcription factor (MITF) were also assayed. Results: Experimental results revealed that treatment with BSE reduced cellular melanin production by approximately 40% compared to the control. Molecular findings supported that suppressed activity and expression of tyrosinase and MITF proteins by BSE were associated with declined cellular melanogenesis. Furthermore, anti-melanogenic effect of BSE ($250{\mu}g/mL$) was similar to that of arbutin, a commonly used whitening agent. Lastly, polyphenols including p-coumaric, ferulic, and vanillic acids were identified in BSE using HPLC analyses. They might be potential active ingredients showing such melanogenesis-reducing effect. Conclusion: BSE was evident to possess favorable anti-melanogenic potency in an in vitro model. As a natural food sourced material, BSE could be an effective depigmentation agent with potential application in pharmaceutical and cosmetic industries.