• Journal of Korea Multimedia Society
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• v.14 no.1
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• pp.103-111
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• 2011

Legacy Java card which adapts a Java platform loads and executes an application when electronics power is provided. However, recently the most Java cards are embedded into a mobile terminal as USIM cards, therefore the power is continually provided for the smart cards. In this case, operation of a Java card system needs to consider its operating system to be advanced in memory management, object management and transaction mechanism. In this paper, we present a high performance Java Card system which is able to have efficient installation, loading and execution of application by applying a new memory management of the smart card that has safe power system.

• The Journal of Korean Society of Virology
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• v.26 no.2
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• pp.141-150
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• 1996

본 연구에서는 $CD4^+$ T 세포를 일회성으로 감염시킬 수 있고 $CD4^+$ T 세포에 HIV-1 envelope 유전자를 전달할 수 있는 바이러스 입자를 생산하는 HIV-1 complementation system을 개발하였고 이 system을 이용하여 $CD4^+$ T 세포에 선택적으로 HIV-1 envelope 당단백질을 발현시켰다. 이 system은 Gag/Gag-Pol expressor와 Env expressor로 구성되어있다. Gag/Gag-Pol expressor는 바이러스 입자 생산에 필요한 구조단백질과 기능단백질을 발현시키지만 packaging signal이 결핍되어 바이러스 입자로 유전자가 들어가지 못하도록 제조되었다. Env expressor는 Tat, Rev와 envelope 당단백질을 발현시키고 packaging signal을 갖고 있어 바이러스 입자로 envelope 유전자가 들어가도록 제조되었다. Gag/Gag-Pol expressor로부터 Gag와 Gag-Pol의 발현은 Rev 단백질을 요구하였고 Env expressor로부터 Rev 단백질 이 제공될 때 Gag와 Gag-Pol 단백질은 효율적으로 발현되었다. Gag/Gag-Pol과 Env expressor로 cotransfection된 COS-1 세포에서 $CD4^+$ T 세포를 일회성으로 감염시킬 수 있는 바이러스 입자가 생산되었다. 생산된 바이러스 입자는 $CD4^+$ T 세포에 HIV-1 envelope 유전자를 전달하여 envelope 당단백질을 발현시켰고 복제 가능한 자손 바이러스의 생성을 유도하지 못하였다. 본 연구에서 개발된 방법은 $CD4^+$ T 세포에서 envelope 당단백질의 기능을 분석하고 관심 있는 유전자를 $CD4^+$ T 세포에 전달하는 바이러스 입자의 생산에 이용할 수 있다.

• 한국신재생에너지학회:학술대회논문집
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• 2007.06a
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• pp.804-807
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• 2007

The acid gas removal (AGR) system was designed and installed to remove $H_2S$ in coal syngas in the pilot-scale coal gasification system for producing chemicals like Dimethyl Ether(DME). The syngas from the coal gasification at the rate of $100{\sim120$ $Nm^3$/hr included pollutants such as fly ash. $H_2S$, COS, $NH_3$, etc. The designed temperature and pressure of the AGR system are below 50oC and 8 kg/$cm^2$. Fe-chelate was used as an absorbent. $H_2S$ was stably removed below 0.5 ppm in the AGR system when the concentration of $H_2S$ was $150{\sim}450$ ppm. The pH of Fe-chelate solution was also stably maintained between $8{\sim}9$. FeMgO absorbent was also tested to remove $H_2S$ in the lab-scale AGR system and $H_2S$ was also removed below 0.5 ppm in the initial operation.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.6
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• pp.1281-1286
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• 2009

Our government operates earned value management system (EVMS)of construction schedule and cost for improving earned management system of the construction projects. but There is two unification Method construction schedule and cost to estimate construction progress currently. This weight a staff work. therefore this study present unification method which operate integrate schedule and cost by using work task. In order to inflect verification and model of schedule and cost, this study develop prototype of EVMS.

• Biomedical Science Letters
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• v.16 no.4
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• pp.279-285
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• 2010

Endogenous cannabinoids (endocannabinoids) display various pharmacological effects including pain control, anti-inflammation, and neuroprotection. The synthesis and release of endocannabinoids are regulated under both physiological and pathological conditions. The main degrading enzyme of endocannabinoid is fatty acid amide hydrolase (FAAH). Therefore we have developed the fluorescence-based assay system for FAAH. We established stable CosM6 cell lines expressing human FAAH. We also synthesized 2-oxo-2H-chromen-7-yl decanoate (DAEC) as a fluorogenic substrate for FAAH. When crude membrane extracts stably expressing FAAH was incubated with DAEC at $25^{\circ}C$, FAAH reacted specifically to DAEC and catalyzes the hydrolysis of DAEC into decanoic acid and highly fluorescent coumarin. Furthermore, the serin hydrolase inhibitor, phenylmethanesulfonylfluoride, inhibited the coumarin release to the reaction buffer in concentration dependent manner. This assay system is suitable for high-throughput screening since this system has simple experimental procedure and measurement method.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.62 no.3
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• pp.332-341
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• 2013

SCADA RTU is a remote control unit equipped in substations for automatic operation of a power system. The RTU monitors and measures the operation status of remote substations to transmits the signals to upper SCADA system and receives control command signals from dispatch center to actuate corresponding apparatus. The RTU receives the standard time from GPS receiver to synchronize the times for all devices and acquires the status event of power apparatus using DIM. Without the time synchronization between GPS and RTU, stable operation of the power system and accurate analysis of the system fault are impossible due to the time error of SOE. In this paper, a test is performed to identify if the operation time of SCADA RTU DIM coincides with the standard time. And we tried to find the reason of error, to minimize the time discrepancy. Through this study, RTU operation time could be synchronized with the standard time within 10[ms] to improve the reliability of SOE data.

• Korean Journal of Environmental Biology
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• v.34 no.2
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• pp.107-115
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• 2016

Currently, there are 442 operating nuclear power plants in the world, and 62 more are under construction. According to this reasoning, the treatment of radioactive waste is important to prevent the environmental ecosystem including humans, animals, and plants. Especially, a leakage of radioactive waste causes not only regional problem but also serious global one. In this study, we demonstrate the effect of radioisotopes (e.g., cesium, strontium, and cobalt) on a 3D culture cell. To develop the 3D cell culture system, we used a 96-well-culture plate with biocompatible agarose hydrogel. Using this method, we can perform the 3D cell culture system with three different cell lines such as HeLa, HepG2, and COS-7. In addition, we conducted a cell viability test in the presence of radioisotopes. Interestingly, the 3D morphological cells showed 42% higher cell viability than those on the 2D against cesium. This result indicates that the 3D platform provides cells morphological and physiological characteristic similar to in vivo grown tissues. Moreover, it overcomes the limitation of conventional cell culture system that can't reflect in vivo systems. Finally, we believe that the proposed approach can be applied a new strategy for simple high-throughput screening and accurate evaluation of metal toxicity assay.

• Communications of the Korean Mathematical Society
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• v.12 no.1
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• pp.157-163
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• 1997

Based on a n-regular polygon $P_n$, we show that $r_n = 1/(2 \sum^{[(n-4)/4]+1}_{j=0}{cos 2j\pi/n)}$ is the ratio of contractions $f_i(1 \leq i \leq n)$ at each vertex of $P_n$ yielding a symmetric gasket $G_n$ associated with the just-touching I.F.S. $g_n = {f_i $\mid$ 1 \leq i \leq n}$. Moreover we see that for any odd n, the ratio $r_n$ is still valid for just-touching I.F.S $H_n = {f_i \circ R $\mid$ 1 \leq i \leq n}$ yielding another symmetric gasket $H_n$ where R is the $\pi/n$-rotation with respect to the center of $P_n$.

• IEMEK Journal of Embedded Systems and Applications
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• v.11 no.5
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• pp.259-265
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• 2016

In this paper, we present an architecture for the fault isolation by applying virtualization-based multi-stack technologies. We propose the simultaneous sharing and switching mechanism using virtualied serial communications. Each guest OS has its own virtual serial device. The distribution module provides communications between the guest OS's through the virtual serial devices and simultaneously detect the liveness of the guest OS. The suggested mechanism has been implemented in VirtualBox and shows satisfactory performance in transmission speed and data sharing capability with virtual RS232.

• 한국신재생에너지학회:학술대회논문집
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• 2007.11a
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• pp.457-460
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• 2007

석탄가스화 기술은 석탄을 고온/고압 조건에서 가스화 반응시켜 CO와 $H_2$가 주성분인 합성가스(syngas)로 전환시키는 기술이다. 그러나 가스화 반응으로 인해 합성가스 내에는 불순물인 $H_2S$, COS, $NH_3$ 등의 오염 물질이 발생하게 되며, 가스터빈의 부식, 촉매의 피독, 전극의 성능 저하 현상 등을 일으켜 효율을 저하시키게 된다. 이에 본 연구에서는 FeMgO 촉매를 제거용매로 사용하여 $H_2S$를 효과적으로 제거하기 위하여 Lab-scale 탈황 설비를 제작하였으며, 석탄 가스화 운전에 연계하여 합성가스 내 포함된 산성가스 정제 특성에 관한 연구를 진행하였다.