As a preliminary study in order to develop new varieties of Hericium species, this study was carried out to investigate the optimal temperature for mycelial growth, to figure out the applicability to sawdust cultivation on Quercus mongolica substrate, and to analyze the antioxidant capacity of ergothioneine and polyphenols in Hericium strains preserved in Korea Forest Research Institute (KFRI). In the results of optimal temperature for mycelial growth of eight Hericium erinaceus, it was $20^{\circ}C$ in a strain (KFRI 842), $25^{\circ}C$ in five strains (KFRI 507, 508, 509, 843, 845), and $30^{\circ}C$ in two strains (KFRI 582, 844). Optimal temperature for mycelial growth of H. coralloides (KFRI 713) was $25^{\circ}C$. Four strains (KFRI 508, 843, 844, 713) out of the total nine Hericium strains showed full mycelium growth within 20 days at the optimal temperature on PDA medium in petri-dish (85 mm in diameter). The other strains have need of more time for full mycelium growth. Mushroom production of H. erinaceus ranged from 215 to 384 g of fresh weight and its dry weight was 7 to 9% of it, whereas that of H. coralloides was 299 g of fresh weight and its dry weight was 10% of it. The contents of ergothioneine and polyphenols of H. erinaceus strains were different by strains and those were in the range of $1.6{\sim}3.7$ mg/g dw. and $5.9{\sim}7.8$ mg/g dw., respectively. On the other hand, those of H. coraloides were in the range of 1.7 mg/g dw. and 3.9 mg/g dw., respectively. From the results of correlation ($R^2$ = 0.1) between ergothioneine and polyphenols in the strains, it was found that the total contents of them differ by strains but the ratio of the two compounds was not very different in the strains.
Purpose: To investigate the degree and effect of cyclooxygenase (COX)-2 expression on the survival of patients with glioblastoma multiforme (GM). Materials and Methods: Between 1997 and 2006, thirty consecutive GM patients treated with surgery and postoperative radiotherapy (dose range: $44{\sim}65.1$ Gy, median dose: 61.2 Gy) were included in the study. Three patients were excluded that discontinued radiotherapy before receiving a dose of 40 Gy due to mental deterioration. The expression of the COX-2 protein in surgical specimens was examined by immunohistochemical analysis. Survival analysis and verification were performed with respect to sex, age, performance status, resection extent, radiotherapy dose, and degree of COX-2 expression using the Kaplan-Meier method and the log rank test. Results: The median length of follow-up was 13.3 months (range:$6{\sim}83$ months). Staining for COX-2 was positive in all patient samples. Staining for COX-2 that was positive for over 75% of the tumor cells was found in 24 patients. Staining for COX-2 that was positive in less than 25% of tumor cells was found in 3 patients (10.0%), staining for COX-2 that was positive in 25 to 50% of tumor cells was found in 1 patient (3.3%), staining for COX-2 that was positive in 50 to 75% of tumor cells was found in 2 patients (6.7%) and staining for COX-2 that was positive in 75 to 100% of tumor cells was found in 24 patients (80.0%). The median survival and two-year survival rate were 13.5 months and 17.5%, respectively. The survival rate was influenced significantly by the degree of resection (tumor removal by 50% or more) and radiotherapy dose (59 Gy or greater) (p<0.05). The median survival of patients with staining for COX-2 that was positive in less than 75% of tumor cells and in at least 75% of tumor cells was 15.5 and 13.0 months, respectively (p>0.05), and the two-year survival for these groups was 33.3 and 13.3%, respectively (p>0.05). Conclusion: The absence of a statistical correlation between the degree of COX-2 expression and survival in GM patients, despite the high rate of COX-2 positive tumor cells in the GM patient samples, requires further studies with a larger series to ascertain the prognostic value of the degree of COX-2 expression in GM patients.
Lee, Hyun Dong;Lee, Jae Min;Lee, Yong Jik;Lee, Young Hwan;Hah, Jeong Ok
Clinical and Experimental Pediatrics
/
v.50
no.8
/
pp.774-780
/
2007
Purpose : The anthracyclines (AC) are widely used chemotherapeutic agents for pediatric cancers. However, the therapeutic use of these agents is limited by their cardiotoxicity. The aim of the present study was to investigate the usefulness of plasma B-type natriuretic peptide (BNP) levels as a marker for AC-induced cardiotoxicity compared to echocardiography in Korean children with cancer. Methods : Fifty-five pediatric cancer patients who had received chemotherapy including AC were enrolled. The cumulative AC doses, clinical symptoms, and two echocardiography parameters, left ventricular fractional shortening (LVFS) and left ventricular ejection fraction (LVEF), were studied and compared with plasma BNP levels. Results : In 55 patients, plasma BNP levels were measured 115 times and echocardiographies were performed 64 times. The median cumulative dose of AC was $325mg/m^2$ (range 120-600; mean 345) and the median plasma BNP level was 10 pg/mL (range 5-950; mean 31). The cumulative AC doses correlated significantly with the plasma BNP levels (P=0.002). The plasma BNP levels correlated significantly with LVFS (P=0.018) and LVEF (P=0.025). Dilated cardiomyopathies were identified in three patients. LVFS and LVEF decreased and plasma BNP levels increased in a patient with acute dilated cardiomyopathy and in that with symptomatic chronic dilated cardiomyopathy. However, LVFS, LVEF and plasma BNP levels were normal in a patient with asymptomatic chronic dilated cardiomyopathy. Conclusion : The results of this study demonstrated that plasma BNP levels could be used as a marker for AC-induced cardiotoxicity; they showed good correlation with echocardiography findings in pediatric cancer patients. Plasma BNP levels may be used for the detection and management of AC-induced cardiotoxicity in Korean children with cancer.
Choi, Don-Hyeok;Choi, Kwang-Soon;Hwang, Gil-Son;Kim, Dong-Sup;Kim, Sea-Won;Kang, Ho
Journal of Korean Society of Environmental Engineers
/
v.31
no.10
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pp.893-900
/
2009
To estimate the contribution of epiphytic algae attached on reed to organic matter production in constructed wetland, primary productivity by epiphytic algae was investigated in two sub-wetlands (Banweol and Donhwa wetlands) of the Sihwa Constructed Wetland (CW) with different chemistry of inflows. Chlorophyll a concentration of epiphytic algae was higher in the Banweol wetland (range:37~3,581 mgChl.a/$m^2$surface stem, average:655 mgChl.a/$m^2$surface stem) than the Donhwa wetland (range:87~2,093 mgChl.a/$m^2$surface stem, average:527 mgChl.a/$m^2$surface stem). In contrast, assimilation number (AN) representing photosynthetic activity was higher in the Donhwa wetland with low TN/TP ratio than the Banweol wetland. A negative correlation (r=0.46) was observed between TN/TP ratios of inflows and AN in two wetlands, implying that high photosynthetic activity of epiphytic algae may be related with low TN/TP ratio. The areal primary productivity ranged from 307 to 2,473 mgC/$m^2$/day in the Banweol wetland and from 756 to 2,096 mgC/$m^2$/day in the Donghwa wetland, showing high productivity in summer. Average primary production was lower in the Banweol wetland (1,166 mgC/$m^2$/day) than the Donghwa wetland (1,467 mgC/$m^2$/day), although the standing crop (as chlorophyll a concentration) was high in the Banweol wetland. This result may be due to the low photosynthetic activity of epiphytic algae in the Banweol wetland with high TN/TP ratio. The annual primary production (300 tonC/year) of epiphytic algae contributed 33% of the total production in the Sihwa CW. An excessive organic matter production in constructed wetland can negatively affect the efficiency of water treatment. Therefore, the role of epiphytic algae should be considered in management of constructed wetland for water treatment.
Today, more chronically ill and handicapped people are being cared for at home by a family member caregiver. The task of caring for a family momber may mean that the caregiver has less time and money and more work which may result in increased fatigue and symptoms of illness. This study was done to examine the well-being of family caregivers. Fifty three family caregivers were interviewed. Concepts were measured using existing tools and included : Burden(25 item 5 point scale), Social sup-port (21 item 7 point scale), Health status defined by a symptom checklist(48 item S point scale), and Well -being defined by a quality of life scale (14 item 7 point scale) and caregiving activities. Data collection was done by interview and Q-sort. Social support and well - being were positively correlated as were symptoms and burden. Symptoms and burden were negatively correlated with social support and well-being. Items on the quality of life scale had a mean score range from 3.09 to 4.96. Quality of life related to income was lowest (3.09) but the desire to use more money for the patient was rated 2.90 on the burden scale where the item means ranged from 0.73 to 3.55. The high mean of 3.55 was for obligation to give care and the low 0.73 was (or not feeling that this was helping the patient. Mean scores for symptoms ranged from 0.26 to 2.15 with the 2.15 being for “worry about all the things that have to be done.” Over half of the patients were dependent for help with some activities of daily living. The caregivers reported doing an average of 3.40 out of five patient care activities including bathing (77.4%), shampooing (67.9%), and washing face and hands (49.1%), and 3.74 out of seven home maintenance activities including laundry (98.1%), cooking (83.0%), and arranging bed-ding(75.5%). The caregivers reported their spouse as one of the main sources of social support, including in times of loneliness and anger The mean score for loneliness as burden was 2.15 and ranked fourth and 31 (58.5%) of the sample reported being lonely recently and not being satisfied with the support received. Similarly anger caused by the patient was given a mean score of 2.13, and anger was reported to have been present recently by 38 (71.7%) of the sample and satis-faction with the support given was low. Having someone to help deal with anger ranked twelfth out of 21 items on the social support scale and had a mean score of 3.98 (range 3.49 to 5.98). Spouses were reported as a major source of social support but the fact that 50% of the caregivers were caring for a spouse, may account for the quality of this source of social support having been affected. These caregivers faced the same problems as others at the same stage of life. but because of the situation, there was a strain on their resources, particularly financial and social. In conclusion it was found that burden is correlated negatively to quality of life and positively to symptoms, but in this sample, symptoms and bur-den were scored relatively low. Does this indicate that the caregivers accept caregiving as part of their destiny and accept the quality of their lives with burden and symptoms just being a part of caregiving\ulcorner Does the correlation between the bur-den and symptoms indicate they are a measure of the same phenomenon or that the sample was of a more mobile, less burdened group of caregivers\ulcorner Quality of life was the one variable that was significant in explaining the varience on burden. Further study is needed to validate the conclusions found in this study but they indicate a need for nurses to ap-proach these caregivers with a plan tailored to each individual situation and to give consideration to interventions directed at improving quality of life and expanding social support networks for those caring for spouses.
Journal of the Korean Society of Marine Environment & Safety
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v.20
no.4
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pp.358-371
/
2014
For the systematic scientific management in Cheonsu Bay of Korea, this study was conducedt to survey the particle composition, organic matter(I.L.) and heavy metals in farming and non-farming areas. The sediment of study area showed feature mixed property by sand, silt and clay. The farming area showed superior by fine-grained sediment, non-farming area showed superior by coarse-grained sediment. The organic pollution of farming area were appeared to be heavily polluted more than non-farming area. The concentration of total nitorgen in sediment was higher farming area than non-farming area. Also, in the case of heavy metals pollution in sediments, farming area was higher than non-farming area. The correlation analysis among to heavy metals, organic matter and particle size was found to have a good interrelationship. For evaluation of heavy metals pollution of sediments, three criteria are applied, Enrichment Factor(EF), Geoaccumulation index(Igeo) and NOAA criteria for sediment. In the case of EF, Heavy metals pollution was appeared to artificial effect all heavy metals if except Cu. In the case of Geoaccumulation index, Cu, Al, Pb was shown zero grade, that is non polluted group, and Cd, Hg, Cr was shown to 0~1 grade, that is mid polluted group, As was shown to 2 grade, that is moderately polluted group. In the case of NOAA, pollution levels of heavy metals except Cd belonged to a group of ERL(Effect range low)~ERM(Effect range median).
Song Kwan-Cheol;Lee Sang-Mo;Yoo Sun-Ho;Ryu Kwan-Sik;Park Moo-Eon
Korean Journal of Agricultural and Forest Meteorology
/
v.2
no.4
/
pp.156-166
/
2000
This study was carried out to provide the information on seasonal variations of water content under highway asphalt pavements which influence on the dynamic behaviour and durability of pavements, and to assess the correlation between water content and soil or meteorological factors. Total eight sites for water content measurement which included fives sites in Kyungbu, two sites in Honam, and one site in Youngdong Highway were selected considering the variations in geology, topology and meteorology factors over all the country. Water contents under asphalt pavements were measured up to 170 cm depth every two week for total 13 months of August 1992 through September 1993 using neutron moisture meter(CPN-503DR). The range of water content ($\theta$$_{w}$) at the upper soils of above 50 cm depth was 7~12% and was not quite different regardless of sites, except for Iseo site. However, soil water contents below 60 or 70 cm depth were significantly different between the measurement sites, that is, the lowest water content was 5% at Kyungsan site and the highest water content was 20% at Iseo site. For all the sites, seasonal variations in water content during the experimental period were little, their range was within only 1 to 4%. Seasonal variations of water content in original or cutting area, which were 4% more or less, were slightly larger than in bedding areas, which were below 2%. Water contents under asphalt pavements had statistically significant positive correlations with silt and clay content in soil, but there were little correlations between water content and meteorological factors such as precipitation, relative humidity, mean air temperature, and wind velocity.
Purpose: Abnormal myocardial perfusion may be caused by ventricular preexcitation, but its location, extent, severity and correlation with accessory pathway (AP) are not established. We evaluated perfusion patterns on myocardial perfusion SPECT and location of AP in patients with WPW (Wolff-Parkinson-White) syndrome. Materials and Methods: Adenosine Tc-99m MIBI or Tl-201 myocardial perfusion SPECT was performed in 11 patients with WPW syndrome. Perfusion defects (PD) were compared to AP location based on ECG with Fitzpatrick's algorithm or electrophysiologic study and radiofrequency catheter ablation. Results: Patients had atypical chest discomfort or no symptom. Risk of coronary artery disease (CAD) was below 0.1 in 11 patients using the nomogram to estimate the probability of CAD. Coronary angiography was performed in 4 patients (mid-LAD 50% in one, normal in others). In 4 patients, AP localization was done by electrophysiologic study and radiofrequency catheter ablation (RFCA). Small to large extent ($11.0{\pm}8.5%$, range:$3{\sim}35%$) and mild to moderate severity ($-71{\pm}42.7%$, range:$-2l7{\sim}-39%$) of reversible (n=9) or fixed (n=1) perfusion defects were noted. One patient with right free wall (right lateral) AP showed normal. PD locations were variable following the location of AP. One patient with left lateral wall AP was followed 6 weeks after RFCA and showed significantly decreased PD on SPECT with successful ablation. Conclusion: Myocardial perfusion defect showed variable extent, severity and location in patients with WPW syndrome. Abnormal perfusion defect showed in most of all patients, but it did not seem to be correlated specifically with location of accessory pathway and coronary artery disease. Therefore myocardial perfusion SPECT should be interpreted carefully in patients with WPW syndrome.
Park, Hyung-Soo;Kim, Ji-Hye;Choi, Ki-Choon;Kim, Hyeon-Seop
Journal of The Korean Society of Grassland and Forage Science
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v.36
no.1
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pp.50-57
/
2016
This study was conducted to determine the effect of mathematical transformation on near infrared spectroscopy (NIRS) calibrations for the prediction of chemical composition and fermentation parameters in corn silage. Corn silage samples (n=407) were collected from cattle farms and feed companies in Korea between 2014 and 2015. Samples of silage were scanned at 1 nm intervals over the wavelength range of 680~2,500 nm. The optical data were recorded as log 1/Reflectance (log 1/R) and scanned in intact fresh condition. The spectral data were regressed against a range of chemical parameters using partial least squares (PLS) multivariate analysis in conjunction with several spectral math treatments to reduce the effect of extraneous noise. The optimum calibrations were selected based on the highest coefficients of determination in cross validation ($R^2{_{cv}}$) and the lowest standard error of cross validation (SECV). Results of this study revealed that the NIRS method could be used to predict chemical constituents accurately (correlation coefficient of cross validation, $R^2{_{cv}}$, ranging from 0.77 to 0.91). The best mathematical treatment for moisture and crude protein (CP) was first-order derivatives (1, 16, 16, and 1, 4, 4), whereas the best mathematical treatment for neutral detergent fiber (NDF) and acid detergent fiber (ADF) was 2, 16, 16. The calibration models for fermentation parameters had lower predictive accuracy than chemical constituents. However, pH and lactic acids were predicted with considerable accuracy ($R^2{_{cv}}$ 0.74 to 0.77). The best mathematical treatment for them was 1, 8, 8 and 2, 16, 16, respectively. Results of this experiment demonstrate that it is possible to use NIRS method to predict the chemical composition and fermentation quality of fresh corn silages as a routine analysis method for feeding value evaluation to give advice to farmers.
This study was performed to analyze the pollutants contaminated in the soils and leaves of ornamental trees along the Namhae Express way. The results obtained were as follow ; 1. The contents of total sulfur in soils were generally high in site 8 with slope way and site 9 having more traffic volume, as 87 ppm and 74 ppm, respectively, 2. The contents of heavy metals in soils were lower than those of industrial areas and urban roads, and Fe and Pb contents were higher in sites having much traffic volume. 3. The contents of soluble sulfur in leaves were in the range of 0.08%-0.25%. and those of Pinus strobus and Cedrus deodara were the highest as 0.25% and 0.23%, respectively, and that of Euonymus japonica was 0.08%, the lowest. 4. In the case of heavy metals concentration in leaves, the contents of Fe, Mn, Pb, Zn and Cu were in the range of 68-340 ppm, 101-463 ppm, 2.4-4.9ppm, 33-60 ppm and 1.8-5.1 ppm, respectively. Except Fe, there was not a wide difference between sites and species. 5. In the contents of soluble S, Pb and Zn in leaves, the sites between Jinju and Masan having generally much traffic showed more contents than between Jinju and Hadong. Therefore, it is inferred that contents of S, Pb, and Zn are positive related to the traffic volume. 6. Only for Pb, there was significant correlation between the heavy metals in soils and the leaves at 1% level.
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