• Asian-Australasian Journal of Animal Sciences
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• v.32 no.7
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• pp.988-995
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• 2019

Objective: This study was conducted to estimate the temperature and microbial changes of corn silages during aerobic exposure. Methods: Kwangpyeongok (KW) and Pioneer 1543 (PI) corn hybrids were harvested at 29.7% of dry matter and chopped to 3 to 5 cm lengths. Homo (Lactobacillus plantarum; LP) or hetero (Lactobacillus buchneri; LB) fermentative inoculants at $1.2{\times}10^5$ colony forming unit/g of fresh forage was applied to the chopped corn forage which was then ensiled in quadruplicate with a $2{\times}2$ (hybrid${\times}$inoculant) treatment arrangement for 100 days. After the silo was opened, silage was sub-sampled for analysis of chemical compositions, in vitro digestibility, and fermentation indices. The fresh silage was continued to determine aerobic exposure qualities by recorded temperature and microbial changes. Results: The KW silages had higher (p<0.01) in vitro digestibilities of dry matter and neutral detergent fiber than those of PI silages. Silages applied with LB had higher (p<0.001) acetate concentration, but lower (p<0.01) lactate concentration and lactate to acetate ratio than those of LP silages. The interaction effect among hybrid and inoculant was detected in acetate production (p = 0.008), aerobic stability (p = 0.006), and lactic acid bacteria count (p = 0.048). The yeast was lower (p = 0.018) in LB silages than that in LP silages. During the aerobic exposure, PI silages showed higher (p<0.05) temperature and mold than KW silages, while LP silages had higher (p<0.05) lactic acid bacteria and yeast than LB silages. Conclusion: The results indicated that the changes of silage temperature during aerobic exposure seems mainly affected by mold growth, while applied LB only enhanced aerobic stability of PI silages.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.4
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• pp.516-519
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• 2009

This study was conducted to investigate the effects of replacing whole crop corn silage (WCCS) with whole crop rice silage (WCRS) in the total mixed ration (TMR) on nutrient intake, milk yield and its composition in Holstein cows. The Chucheong rice variety (Oryza sativa L. Japonica) and corn (Pioneer 32 P75) were harvested at yellow-ripe stage and were ensiled in round bales and in trench silos, respectively. Two TMR containing either WCCS or WCRS were prepared. These diets were randomly assigned to 16 midlactating Holstein cows (8 cows per treatment) and were fed for 120 days. The first 20 days were used for dietary adaptation and for the next 100 days daily feed intake, milk yield and its composition were recorded. The pH, lactic acid, NDF, ADF, CP, Ca and P contents were similar in WCRS and WCCS. The DM, ash and EE contents of WCRS were greater compared with WCCS. Nutrient (DM, NDF, TDN and CP) intakes were similar in cows fed WCCS- and WCRS-based TMR. Daily and 4% fat corrected milk yield were not affected by the treatments. Milk composition (percent milk fat, protein, lactose and total solids) was similar in cows fed either WCCS- or WCRSbased TMR. The concentration of milk urea N was greater in cows fed WCRS-based TMR than those fed WCCS-based TMR. In conclusion, round-baled WCRS can replace WCCS in the diet of mid- to late-lactating Holsteins without any deleterious effects on feed consumption, milk yield and its composition. The present findings raise the possibility that WCRS can be used as an alternative roughage source in the diets of dairy cows in countries with surplus rice production.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.5
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• pp.620-631
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• 2015

A total of 59 lactic acid bacteria (LAB) strains were isolated from corn stover silage. According to phenotypic and chemotaxonomic characteristics, 16S ribosomal DNA (rDNA) sequences and recA gene polymerase chain reaction amplification, these LAB isolates were identified as five species: Lactobacillus (L.) plantarum subsp. plantarum, Pediococcus pentosaceus, Enterococcus mundtii, Weissella cibaria and Leuconostoc pseudomesenteroides, respectively. Those strains were also screened for antimicrobial activity using a dual-culture agar plate assay. Based on excluding the effects of organic acids and hydrogen peroxide, two L. plantarum subsp. plantarum strains ZZU 203 and 204, which strongly inhibited Salmonella enterica ATCC $43971^T$, Micrococcus luteus ATCC $4698^T$ and Escherichia coli ATCC $11775^T$ were selected for further research on sensitivity of the antimicrobial substance to heat, pH and protease. Cell-free culture supernatants of the two strains exhibited strong heat stability (60 min at $100^{\circ}C$), but the antimicrobial activity was eliminated after treatment at $121^{\circ}C$ for 15 min. The antimicrobial substance remained active under acidic condition (pH 2.0 to 6.0), but became inactive under neutral and alkaline condition (pH 7.0 to 9.0). In addition, the antimicrobial activities of these two strains decreased remarkably after digestion by protease K. These results preliminarily suggest that the desirable antimicrobial activity of strains ZZU 203 and 204 is the result of the production of a bacteriocin-like substance, and these two strains with antimicrobial activity could be used as silage additives to inhibit proliferation of unwanted microorganism during ensiling and preserve nutrients of silage. The nature of the antimicrobial substances is being investigated in our laboratory.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.1
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• pp.22-29
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• 1991

This study was experimented to obtain the basic information on the changeable aspect and improvement of soil fertility in newly-reclaimed sloped land. Silage corn was cultivated under the six different treatments for 4 years. The relation between the amount or ratio of annual changes of soil physico-chemical properties and yield of silage corn were analyzed. Soil bulk density was decreased in 3rd year at topsoil, but that decreased in 4th year at subsoil. Soil organic matter also decreased in 2nd year at topsoil, and decreased continuously at subsoil. Bulk density and hardness of soil depths showed significant negative simple correlation with dry matter yield and cation exchange capacity showed positive. Correlation coefficient of chemical properties with dry matter yield were low. The range of annual changes of moisture percent, hardness and organic matter were wider than the other properties. The significantly different of physical properties were higher than the chemical properties, and those of topsoil were higher than subsoil. According to multiple regression between yield and physico-chemical properties of subsoil, bulk density and cation exchange capacity were in the greatest contribution at the variations, but bulk density was greatest at the ratios.

• Proceedings of the Korean Society of Crop Science Conference
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• 1986.06a
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• pp.72-73
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• 1986

• Journal of The Korean Society of Grassland and Forage Science
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• v.16 no.4
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• pp.299-306
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• 1996

This experiment was conducted from 1991 to 1993 at the forage experimental field, College of Agriculture and Life Sciences, SNU, Suweon to investigate the effects of previous crop residues on growth and yield of succeeding corn(2ea mays L.) and also to determine the best double cropping system necessary to maximize the total dry matter yield of winter forage crops plus corn for silage. In this experiment, treatments consisted of no crop as a control, late maturing Kodiak rye(Seca1e cereale L.), Bamapoli forage rape(Brassica napus Subsp. oleifera L.), G-sprinter oats(Avem sativa L.), Chief crimson clover(Trifolium incarnatum L.), Jackson Italian ryegrass(lo1ium rnultiJlorum L.), Vantage vetch(Vicia sativa L.) and early maturing Koolgrazer rye(Seca1e cereale L.). Corn leaf number and silking date were not significantly affected by previous crop residues, but the leaf number of corn following Italian ryegrass was reduced by 1.9 relative to no previous crop. The plant height and LA1 were significantly reduced during early development when corn followed Italian ryegrass and late maturing Kodiak rye (p<0.05). The dry matter and estimated TDN yields of corn were significantly reduced when corn followed Italian ryegrass and late maturing Kodiak rye. The yield reductions for corn following Italian ryegrass, late maturing Kodiak rye and early maturing Koolgrazer rye, relative to com when no previous crops were planted, were 34, 17 and 8%, respectively. Therefore, the reduction in corn growth and yield in this experiment could be explained by an allelopathic effect resulting from the Italian ryegrass and rye residues. The highest total dry matter yield of 30,509kg/ha was obtained from an early maturing rye-corn double cropping sequence in combination among the eight cropping systems.

• Journal of Microbiology and Biotechnology
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• v.26 no.4
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• pp.739-747
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• 2016

The rational utilization of crop straw as a raw material for natural gas production is of economic significance. In order to increase the efficiency of biogas production from agricultural straw, seasonal restrictions must be overcome. Therefore, the potential for biogas production via anaerobic straw digestion was assessed by exposing fresh, silage, and dry yellow corn straw to cow dung liquid extract as a nitrogen source. The characteristics of anaerobic corn straw digestion were comprehensively evaluated by measuring the pH, gas production, chemical oxygen demand, methane production, and volatile fatty acid content, as well as applying a modified Gompertz model and high-throughput sequencing technology to the resident microbial community. The efficiency of biogas production from fresh straw (433.8 ml/g) was higher than that of production from straw silage and dry yellow straw (46.55 ml/g and 68.75 ml/g, respectively). The cumulative biogas production from fresh straw, silage straw, and dry yellow straw was 365 l^-1 g^-1 VS, 322 l^-1 g^-1 VS, and 304 l^-1 g^-1 VS, respectively, whereas cumulative methane production was 1,426.33%, 1,351.35%, and 1,286.14%, respectively, and potential biogas production was 470.06 ml^-1 g^-1 VS, 461.73 ml^-1 g^-1 VS, and 451.76 ml^-1 g^-1 VS, respectively. Microbial community analysis showed that the corn straw was mainly metabolized by acetate-utilizing methanogens, with Methanosaeta as the dominant archaeal community. These findings provide important guidance to the biogas industry and farmers with respect to rational and efficient utilization of crop straw resources as material for biogas production.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.3
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• pp.146-154
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• 2022

This experiment was conducted to evaluate the growth characteristics and productivity of silage corn varieties developed in Korea. Corn cultivation was carried out using the experimental field in the Pyeongchang campus of Seoul National University (550 m above sea level). There have 10 domestic cultivars (Gwangpyeongok, Dacheongok, Yanganok, Jangdaok, Cheongdaok, Daanok, Sinhwangok, Sinhwangok II, Pyeonggangok, and Hwangdaok) with one imported cultivar (P1543) which tested as a control, and randomized block design with three replications. Among the 100-grains weight of the seeds, Dacheongok was the heaviest, and the germination rate for each variety was 74.6% on average, while that of Daanok and Sinhwangok were over 90%. Sinhwangok was the fastest in tasseling and silking date. The number of days required to be silking date was as slow as 85 days in Dacheongok, Cheongdaok and Pyeonggangok, and as fast as 80 days or less in Sinhwangok, Sinhwangok II and Hwangdaok. The plant height of P1543 was the highest as 344cm, and Hwangdaok and Daanok were short. In terms of the ratio of ears, Daanok had the highest rate of 60.18%, and Jangdaok and Dacheongok had the lowest. There was no significant difference in dry matter content in stover, but P1543 was generally higher in ear and total dry matter content. The dry matter yield was highest in P1543, and the yield of TDN was significantly higher in P1543 and Yanganok. There was a significant difference in the crude protein content of ears and the dry digestibility of stems (p<0.05), while there was no significant difference in the content of each part or element. Combining the above results, Yanganok was the highest in terms of yield, and Dacheongok, Sinhwangok and Pyeonggangok were also recommended for domestically grown corn varieties in the mountainous regions of Gangwon-do.

• Journal of The Korean Society of Grassland and Forage Science
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• v.32 no.3
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• pp.265-274
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• 2012

This study was carried out to investigate the growth characteristics, yield and chemical compositions of corn hybrids for silage according to ridging times in the paddy field cultivation. The experimental design was arranged in a randomized block design with five treatments and three replication. The ridging times of five treatments were untreated (C), 7 leaves (T1: 31 days), 8 leaves (T2: 37 days), 9 leaves (T3: 42 days) and 10 leaves stage (T4: 48 days after sowing) of silage corn. The ridging treatments (T1, T2, T3, T4) were higher (p<0.01) dry matter yield and TDN yield than the unridging treatment (C). However, there were no significant differences among T1, T2, T3 and T4 treatments. The Crude protein content of unridging treatment was decreased (p<0.01), while NDF content was increased (p<0.01) compared to ridging treatments. The contents of mineral and amino acid in ridging treatments were higher than unridging treatment (p<0.05, p<0.01). In particular, mineral and amino acid contents were the highest in T4 and T1, respectively (p<0.01). Based on the above results, ridging work is to be increased the yield quantity and nutritional value of silage corn. And the best result was obtained in T4 treatment of ridging treatments.

• Journal of The Korean Society of Grassland and Forage Science
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• v.43 no.3
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• pp.148-155
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• 2023

This study was aimed to isolate bacterial inoculants producing chitinase and evaluate their application effects on corn silage. Four corn silages were collected from four beef cattle farms to serve as the sources of bacterial inoculants. All isolates were tested against Fusarium graminearum head blight fungus MHGNU F132 to confirm their antifungal effects. The enzyme activities (carboxylesterase and chitinase) were also measured to isolate the bacterial inoculant. Based on the activities of anti-head blight fungus, carboxylesterase, and chitinase, L. buchneri L11-1 and L. paracasei L9-3 were subjected to silage production. Corn forage (cv. Gwangpyeongok) was ensiled into a 10 L mini silo (5 kg) in quadruplication for 90 days. A 2 × 2 factorial design consists of F. graminearum contamination at 1.010⁴ cfu/g (UCT (no contamination) vs. CT (contamination)) and inoculant application at 2.1 × 10⁵ cfu/g (CON (no inoculant) vs. INO (inoculant)) used in this study. After 90 days of ensiling, the contents of CP, NDF, and ADF increased (p<0.05) by F. graminearum contamination, while IVDMD, acetate, and aerobic stability decreased (p<0.05). Meanwhile, aerobic stability decreased (p<0.05) by inoculant application. There were interaction effects (p<0.05) on IVNDFD, NH3-N, LAB, and yeast, which were highest in UCT-INO, UCT-CON, CT-INO, and CT-CON & INO, respectively. In conclusion, this study found that mold contamination could negatively impact silage quality, but isolated inoculants had limited effects on IVNDFD and yeast.