• Carbon letters
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• v.13 no.3
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• pp.161-166
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• 2012

Carbon materials were synthesized by pyrolysis from fibers of Corn-straw (Zea mays), Rice-straw (Oryza sativa), Jute-straw (Corchorus capsularis) Bamboo (Bombax bambusa), Bagass (Saccharum officinarum), Cotton (Bombax malabaricum), and Coconut (Cocos nucifera); these materials were characterized by scanning electron microscope, X-ray diffraction (XRD), and Raman spectra. All carbon materials are micro sized with large pores or channel like morphology. The unique complex spongy, porous and channel like structure of Carbon shows a lot of similarity with the original anatomy of the plant fibers used as precursor. Waxy contents like tyloses and pits present on fiber tracheids that were seen in the inherent anatomy disappear after pyrolysis and only the carbon skeleton remained; XRD analysis shows that carbon shows the development of a (002) plane, with the exception of carbon obtained from bamboo, which shows a very crystalline character. Raman studies of all carbon materials showed the presence of G- and D-bands of almost equal intensities, suggesting the presence of graphitic carbon as well as a disordered graphitic structure. Carbon materials possessing lesser density, larger surface area, more graphitic with less of an $sp^3$ carbon contribution, and having pore sizes around $10{\mu}m$ favor hydrogen adsorption. Carbon materials synthesized from bagass meet these requirements most effectively, followed by cotton fiber, which was more effective than the carbon synthesized from the other plant fibers.

• Journal of Microbiology and Biotechnology
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• v.31 no.10
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• pp.1446-1454
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• 2021

Herein, we cloned and expressed an endo-β-1,4-glucanase gene (celA1805) from Bacillus subtilis B111 in Escherichia coli. The recombinant celA1805 contains a glycosyl hydrolase (GH) family 8 domain and shared 76.8% identity with endo-1,4-β-glucanase from Bacillus sp. KSM-330. Results showed that the optimal pH and temperature of celA1805 were 6.0 and 50℃, respectively, and it was stable at pH 3-9 and temperature ≤50℃. Metal ions slightly affected enzyme activity, but chemical agents generally inhibited enzyme activity. Moreover, celA1805 showed a wide substrate specificity to CMC, barley β-glucan, lichenin, chitosan, PASC and avicel. The K_m and V_max values of celA1805 were 1.78 mg/ml and 50.09 µmol/min/mg. When incubated with cellooligosaccharides ranging from cellotriose to cellopentose, celA1805 mainly hydrolyzed cellotetrose (G4) and cellopentose (G5) to cellose (G2) and cellotriose (G3), but hardly hydrolyzed cellotriose. The concentrations of reducing sugars saccharified by celA1805 from wheat straw, rape straw, rice straw, peanut straw, and corn straw were increased by 0.21, 0.51, 0.26, 0.36, and 0.66 mg/ml, respectively. The results obtained in this study suggest potential applications of celA1805 in biomass saccharification.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.8
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• pp.1124-1131
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• 2012

Four Thai - rumen fistulated male swamp buffaloes (Bubalus bubalis), about four years old with $400{\pm}20kg$ liveweight, were randomly assigned according to a $4{\times}4$ Latin square design to receive dietary treatments. The treatments were: ground corn cob (GCC) replacement for cassava chip (CC) in concentrate at 0% (T1); GCC replacement at 33% (T2); GCC replacement at 67% (T3); and GCC replacement at 100% (T4), respectively. During the experiment, concentrate was offered at 0.5% BW while 5% urea-treated rice straw was given at ad libitum. The result revealed that there was no effect of GCC replacement on DMI among treatments. In addition, digestibilities of DM, OM and CP were not different while aNDF linearly increased with an increasing level of GCC replacement. However, GCC replacement did not affect rumen fermentation such as ruminal pH, $NH_3$-N and VFA concentration; except C3 proportion which was the highest at 33% replacement while the lowest was at 100% replacement. All replacements of GCC resulted in similar protozoal and bacterial populations and microbial protein synthesis (MPS). Purine derivatives (PD) concentration in urine and PD to creatinine (PDC) index were varied with time of urination and among treatments at 0 to 8 and 8 to 16 h post feeding and higher values were shown among the GCC replacement groups. However at 16 to 24 h-post feeding, it was untraceable. In addition, creatinine concentration was similar among all treatments at every sampling time. Based on the above results, GCC can be used as an energy source for swamp buffalo fed with rice straw. Spot sampling of urine can be used for purine derivatives determination.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 1998.10a
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• pp.473-478
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• 1998

Dead animals amended with agricultural rersidues are utilized ai a compost to promote soil fertility and reduce environmental pollution by traditional methods of livestock mortalities. The full-scale bin composting swine mortality in roofed system with three amendments was very successful in reaching sufficient temperature above 55$^{\circ}C$ long enough for pathogen kill. The average temperature of tile compost material in dead swine amended with corn stover increased rapidly to 64$^{\circ}C$ on the 2nd day after primary composting and dropped to near ambient temperature on the 140th day of composting. The composting temperature of corn stover and wheat straw is more efficient for swine carcass composting than that of sawdust.

• Journal of Animal Environmental Science
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• v.5 no.1
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• pp.45-52
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• 1999

The purpose of this study is to provide information for the livestock for the livestock carcass composting operation in agricultural waste management. Burial, landfilling and incineration of livestock carcass may have environmental regulatory and economic liabilities. Dead animals amended with agricultural residues can be composted and used to promote soil fertility and reduce environmental pollution. In this study we evaluated the effect of amendments on the primary and secondary composting reaction and stability of compost. The full-scale composting bin of swine carcass in roofed system with three amendments was adequate in reaching sufficient temperature above 55$^{\circ}C$ long enough to kill the pathogen. The average temperature of the compost material in dead swine amended with corn stover increased rapidly to 64$^{\circ}C$ on the 2nd day after primary composting and dropped to near ambient temperature on the 140th day of composting. The composting with of corn stover and wheat straw are more efficient for swine carcass composting than that of sawdust. Therefore, the amendment property is an important factor in the design of composting facility.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.1
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• pp.68-72
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• 2004

The effects of the addition of yeast on in vitro roughage degradability and methane production were investigated in order to clarify the effects of yeast on the rumen microbes and to establish methods of rumen manipulation. Three roughages (whole crop corn, rice straw and Italian ryegrass) were incubated for 3, 6, 12 and 24 h with or without dried beer yeast following the method described by Tilley and Terry. Using the same method, these roughages were incubated with or without yeast extract, albumin or purified DNA. In vitro methane production was measured with or without dried beer yeast at 12 and 24 h. The degradability of yeast was found to be 57 and 80% at 12 and 24 h, respectively. The rate of degradation of fraction b was 6.16%/h. There was a significant increase in roughage degradability at 6 h (p<0.05), 12 h (p<0.05) and 24 h (p<0.01) by dried yeast addition. The degradability of all three roughages was higher in the samples treated with yeast extract than in the no addition samples except in the case of rice straw incubated for 12 h. Nevertheless, the magnitude of increment was smaller with the addition of yeast extract than without the addition of yeast. With the addition of purified DNA, there were significant increases in roughage degradability at 6 h (p<0.01), 12 h (p<0.01) and 24 h (p<0.05); however, higher degradability values were detected in the samples to which albumin was added, particularly at 6 h. If the degradability values of the no addition samples with those of samples containing yeast, yeast extract, DNA and albumin were compared, the largest difference was found in the samples to which yeast was added, although it is worth noting that higher values were observed in the yeast extract samples than in the DNA or albumin samples, with the exception of the case of rice straw incubated for 24 h. Methane production was significantly increased at both 12 and 24 h incubation. The increment of roughage degradation and methane production brought about by the addition of dried beer yeast to the samples was thought to be due to the activation of rumen microbes. Water soluble fraction of yeast also seemed to play a role in ruminal microbe activation. The increment of degradability is thought to be partially due to the addition of crude protein or nucleic acid but it is expected that other factors play a greater role. And those factors may responsible for the different effects of individual yeast on ruminal microbes.

• Korean Journal of Poultry Science
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• v.12 no.2
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• pp.97-105
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• 1985

Fifteen 1-yr old roosters were employed in order to investigate the influence of various dietary fibers on the water and Na metabolism of chicken. Wheat bran, pure cellulose and ground rice straw replaced corn in the control diet at 10% level; Pectin replaced corn at 8% level. Three roosters were allotted per dietary treatment and one rooster per replicate. The limit of probability accepted as being significant was P$\leq$.05. The dry matter metabolizabilities of cellulose and rice straw diets were significantly lower, and those of control and pectin diets were significantly higher than that of the wheat bran diet. Pectin fed roosters seemed to drink more water than did the other birds. Also, excreta from roosters fed the pectin diet were significantly wetter than those from other treatments. However, no difference in water intake and fecal moisture were found when !he roosters were colostomized. It was also found that the colostomized birds drank more water than did intact ones. Data of water holding capacity and bulk density of dried excreta showed that cellulose, rice straw and wheat bran in decreasing order were strong in fibrous property. There were no significant differences in C$_{Na+}$ , Cosm and C$_{{H_2}O}$ among all treatments. When the values of urine volume, urine osmolality and C$_{{H_2}O}$ were put together, it can be concluded that 11 out of 15 roosters developed diuresis. More severe diuretic symptoms were developed in roosters when they were fed more fibrous diets. In conclusion, when the four dietary fibers were graded in terms of fibrous property, it turned out to be pectin, cellulose, rice straw and wheat bran in decreasing order.

• Journal of Animal Science and Technology
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• v.44 no.3
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• pp.341-350
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• 2002

Twenty four Hanwoo bulls(mean initial body weight 162$\pm$8.1kg) were employed to induce the regression equations on body weight gain and dry matter intake. The diets were fed in a 3${\times}$4 factorial arrangement; three roughage sources(rice straw, hay, corn silage) and four roughage ratios in ration(100, 60, 40, 20%). Bulls were alloted by incomplete turn over design with four replications. Daily body weight gain was greatest in bulls fed the corn silage as a roughage source and the 20% of roughage ratio, respectively. Bulls fed hay or corn silage only as feed kept in their body weight gain during the whole experimental period, however those fed rice straw only were in a negative body gain hereafter fatteningⅠperiod(350kg of body wt.). Dry matter intake per metabolizable body weight was decreased with increasing body weight. Dry matter intake was greatest in bulls fed the corn silage as a roughage source and the 20% of roughage ratio, respectively. The regression equations on daily body weight gain(DG, kg) and dry matter intake(DMI, kg/day) according to body weight(BW, kg) of Hanwoo bulls were derived as follows; DG = －0.842 + 17.5DMI/$BWkg^{0.75}$ DMI = 4.125 + 0.013BW + 1.075DG － 0.045NDF where, NDF is the percentage of neutral detergent fiber in ration.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.3
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• pp.356-364
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• 2009

The current ruminant feeding models require parameterization of the digestion kinetics of carbohydrate fractions in feed ingredients to estimate the supply of nutrients from a ration. Using an automated gas production technique, statistically welldefined digestion rate of carbohydrate, including soluble carbohydrate, can be estimated in a relatively easy way. In this study, the gas production during in vitro fermentation was measured and recorded by an automated gas production system to investigate degradation kinetics of carbohydrate fractions of a wide range of ruminant feeds: corn silage, rice straw, corn, soybean hull, soybean meal, and cell mass from lysine production (CMLP). The gas production from un-fractionated, ethanol insoluble residue and neutral detergent insoluble residue of the feed samples were obtained. The gas profiles of carbohydrate fractions on the basis of the carbohydrate scheme of the Cornell Net Carbohydrate and Protein System (A, B1, B2, B3 and C) were generated using a subtraction approach. After the gas profiles were plotted with time, a curve was fitted with a single-pool exponential equation with a discrete lag to obtain kinetic parameters that can be used as inputs for modern nutritional models. The fractional degradation rate constants (Kd) of corn silage were 11.6, 25.7, 14.8 and 0.8%/h for un-fractioned, A, B1 and B2 fractions, respectively. The values were statistically well estimated, assessed by high t-value (>12.9). The Kd of carbohydrate fractions in rice straw were 4.8, 21.1, 5.7 and 0.5%/h for un-fractioned, A, B1 and B2 fractions, respectively. Although the Kd of B2 fraction was poorly defined with a t-value of 4.4, the Kd of the other fractions showed tvalues higher than 21.9. The un-fractioned corn showed the highest Kd (18.2%/h) among the feeds tested, and the Kd of A plus B1 fraction was 18.7%/h. Soybean hull had a Kd of 6.0, 29.0, 3.8 and 13.8%/h for un-fractioned, A, B1 and B2, respectively. The large Kd of fraction B2 indicated that NDF in soybean hull was easily degradable. The t-values were higher than 20 except for the B1 fraction (5.7). The estimated Kd of soybean meal was 9.6, 24.3, 5.0%/h for un-fractioned, A and B1 fractions, respectively. A small amount of gas (5.6 ml at 48 ho of incubation) was produced from fermentation of CMLP which contained little carbohydrate. In summary, the automated gas production system was satisfactory for the estimation of well defined (t-value >12) kinetic parameters and Kd of soluble carbohydrate fractions of various feedstuffs that supply mainly carbohydrate. The subtraction approach, however, should be applied with caution for some concentrates, especially those which contain a high level of crude protein since nitrogen-containing compounds can interfere with gas production.

• Journal of Animal Science and Technology
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• v.58 no.2
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• pp.9.1-9.5
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• 2016

Background: Literature is lacking on the effects of feeding by-product feed (BF)-based silage on rumen fermentation parameters, nutrient digestion and nitrogen (N) retention in sheep. Therefore, this study was conducted to determine the effect of replacing rye straw with BF-based silage as a roughage source on ruminal parameters, total-tract apparent nutrient digestibility, and N balance in sheep. Methods: The by-product feed silage was composed of spent mushroom substrate (SMS) (45 %), recycled poultry bedding (RPB) (21 %), rye straw (11 %), rice bran (10.8 %), corn taffy residue (10 %), protected fat (1.0 %), bentonite (0.6 %), and mixed microbial additive (0.6 %). Six sheep were assigned randomly to either the control (concentrate mix + rye straw) or a treatment diet (concentrate mix + BF-based silage). Results: Compared with the control diet, feeding a BF-based silage diet resulted in similar ruminal characteristics (pH, acetate, propionate, and butyrate concentrations, and acetate: propionate ratio), higher (p < 0.05) ruminal NH3-N, higher (p < 0.05) ether extract digestibility, similar crude protein digestibility, lower (p < 0.05) dry matter, fiber, and crude ash digestibilities, and higher (p < 0.05) N retention (g/d) Conclusion: The BF-based silage showed similar energy value, higher protein metabolism and utilization, and lower fiber digestion in sheep compared to the control diet containing rye straw.