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Isolation of Nematophagous Fungi against Root-knot Nematode and Their Growth in Vitro (뿌리혹 선충에 대한 기생 천적 진균 분리 및 이들의 생장에 미치는 환경조사)

  • 정미정;김희규
    • Korean journal of applied entomology
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    • v.27 no.3
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    • pp.149-158
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    • 1988
  • Nematophagous fungi were successfully isolated by baited plating, centrifugation technique of soil, and direct isolation from naturally ingested nematodes. Predominant seven fungi isolated were identified as Artheobotrys arthroboteyides, A.conoides, A. oligospora, Dactylella lobata, Fusatium oxysporum, Monacrosporium ellopsoporum and Harposporium anguillu-lae. Of these, six fungi were tested for cultural characteristics except. H, anguillulae, extre-mely fastidious fungus in artificial media. Among 14 media tested in this experiment, Corn-meal Agar (CMA) and Oatmeal Agar (OMA) were the most suitable media for growing all six nematophagous fungi. Weakly saprophytic M. ellipsospoyum also grew vigoroualy on these two media. The radial growth, dry weight and sporulation of the fungi tested were quite diverse depending on the culture media. D. lobata revealed good growth and abundantly sporulated on Glucoes Peptone Agar (GPA). Although over-all growth of F, oxysporum was not satisfactory on Sucrose Nitrate Agar (SNA), the sporulation was best on this medium. Optimum conditious for mycelial growth and sporulation of nematophagous fungi ranged pH 5-8 and 20-$30^{\circ}C$ on SNA. D. lobata and F, oxysporum grew vigorously and most profusely sporulated on all media tested. They turned out an most promising biocontrol agents for their aggressive growth and sporulation over the ranges of temperature and pH ranges.

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A Study on Vegetable Intakes and Dietary Habits of Middle School Students in Chungnam (충남지역 중학생의 채소 섭취실태와 식습관에 관한 연구)

  • Cho, Hyun-Sun;Kim, Myung-Hee;Choi, Mi-Kyeong
    • Korean Journal of Community Nutrition
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    • v.15 no.4
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    • pp.525-535
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    • 2010
  • The purpose of this study was to provide basic data for nutritional education for juveniles' desirable vegetable intake by analyzing their vegetable intake, dietary habit and nutrient intakes according to gender of middle school students in Chungnam. The average age of the subjects was 15.0, height was 162.5 cm, weight was 53.5 kg and their average BMI was $20.1kg/m^2$. The frequency of eating supper in girls was lower than that in boys. Many respondents answered that a meal-time was 10~20 minutes. Without gender difference, more than 90% respondents thought that vegetables were good for health. As for the preference of vegetable, subjects responded, "I am in the middle", "I like them", "I dislike them", "I like them very much", and "I dislike them very much" in order, without gender difference. The frequent eaten leaf vegetable was Chinese cabbage, the frequent eaten fruit vegetable was cucumber, and the frequent eaten root vegetable was radish. The favorite leaf vegetable was lettuce, the favorite fruit vegetable was corn, and the favorite root vegetable was sweet potato. The preference degrees of taro and ginger were very low. The reasons why they liked a vegetable were that it was delicious and they ate it at home often. In addition, the reason why they disliked vegetables was that they are untasty in flavor or texture and it showed that many students had a prejudice that vegetables were untasty. The intakes of plant protein, dietary fiber, ash and INQs of dietary fiber, calcium, vitamin C, folate, vitamin E in the group with high preference of vegetables were significantly higher than those of low preference group. The study results indicate that intake frequency and preference of root vegetables in juveniles are low and the major reasons of these results are taste and eating experience of vegetables. In addition, the intake amounts of dietary fiber and folate are poor in the subjects with low preference of vegetables. Therefore, families and schools should make efforts that juveniles can recognize the importance of vegetable intake and select various vegetables properly through the development of cooking methods and systematic nutrition education.

Effects of Fruiting Productivity of Grifola frondosa Using Bottle Cultivation according to Different Substrate Composition (잎새버섯에서 배지조성이 병재배 자실체 생산성에 미치는 영향)

  • Kim, Jeong-Han;Jeon, Dae-Hoon;Kang, Young-Ju;Jeoung, Yun-Kyeoung;Lee, Yun-Hae;Chi, Jeong-Hyun
    • The Korean Journal of Mycology
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    • v.44 no.3
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    • pp.150-154
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    • 2016
  • To determine a favorable substrate formulation for Grifola frondosa, physicochemical conditions, culture properties, and yields according to various substrate formulations were investigated. Based on these analyses, T4 (80:5:15 ratio of oak sawdust to dried bean-curd refuse to corn husk) resulted in a shorter cultivation period and higher yields (weight of fresh mushrooms harvested at maturity) than those of other treatments. The physicochemical properties of T4 were pH 5.4, 2.4% crude fat contents, 54 C/N ratio, 74.3% porosity, and 0.26 g/cm3 bulk density. These results emphasize the importance of optimal substrate development on the production efficiency of G. frondosa mushrooms and have implications for commercial applications.

A Study on the Antioxidative and Antimicrobial Activities of the Chopi(Zanthoxylum Pipperitum DC.) Solvent Extracts (초피 용매 추출물의 항산화성 및 항균성에 관한 연구)

  • Ahn, Myung-Soo;Kim, Hyun-Jeung;Han, Mi-Nam;Won, Jong-Sook
    • Journal of the Korean Society of Food Culture
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    • v.19 no.2
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    • pp.170-176
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    • 2004
  • The antioxidative and antimicrobial activity were carried on the Chopi(Zanthoxylum pipperitum) extracts by six kinds of solvents in order to find out new natural food additives. Six solvents were used methanol(MeOH), n-hexan(hexane), chloroform$(CHCl_3)$, ethylacetate(EtOAc), buthanol(BuOH), and water(water) and methanol extract(MEex), hexan extract(HEex), chloroform extract(CHex), ethylacetate extract(EAex), and buthanol extract(BUex), water extract(WAex). The antioxdative activities of them extracts were determined by peroxide value(POV), conjugated diene value(CDV) of corn germ oil storaged for 30 days at $60{\pm}2^{\circ}C$. These extracts were added as 0.02, 0.1, 0.2% of each extracts and compared with ${\alpha}-tocopherol$, and BHT. The antioxidative activities of 0.02% extract were as follows in decreasing order BUex > WAex, BHT, MEex > HEex, EAex, CHex > TOC, and control. While, BUex and CHex among these extracts were shown to be had antimicrobial effects on the microorganism such as Escheria coli, Salmonella typhimurium, Psedomonas aeruginosa, Staphylococcus aureus, and Listeria monocytogenes. Finally to find out the preference of chopi we had made Yugwanzageon (a kind of shallow fried meat ball)by adding chopi powder. The result were similar to control(not added chopi powder) in case of 0.1% chopi adding Yugwanzageon.(p<0.05) Therefore it was thought to be possible that chopi powder was used Yugwanzageon preperation.

Selective Action of Root-Treated Oxyfluorfen and Chlomethoxynil (근부처리(根部處理) Oxyfluorfen과 Chlomethoxynil의 선택작용성(選擇作用性))

  • Lee, Jeung-Joo
    • Korean Journal of Weed Science
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    • v.14 no.2
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    • pp.107-111
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    • 1994
  • Selective action of root-treated oxyfluorfen [(2-chloro-4-thrifluoromethylphenyl)-3'-ethoxy-4'-nitrophenyl ether] and chlomethoxynil [2, 4-dichlorophenyl-3'-methoxy-4'-nitrophenyl ether] were investigated. Oxyfluorfen showed greater activity to all plant species than chlomethoxynil. $^{14}C$-oxyfluorfen was little metabolized in roots of the plant species and more slowly absorbed than $^{14}C$-chlomethoxynil. These results suggest that herbicidal activity of oxyfluorfen at the site of action is higher than chlomethoxynil. In the tested plants, rice, barnyardgrass, sorghum, and corn were absorbed less of the oxyfluorfen and chlomethoxynil than the broad leaf plant species. However, no clear relationship was observed between a degree of tolerance and absorption and metabolism of both herbicides by the plant species.

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Development of Glucoamylase & Simultaneous Saccharification and Fermentation Process for High-yield Bioethanol (고효율 바이오 에탄올 생산을 위한 당화효소 개발 및 동시당화발효 공정 연구)

  • Choi, Gi-Wook;Han, Min-Hee;Kim, Yule
    • KSBB Journal
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    • v.23 no.6
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    • pp.499-503
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    • 2008
  • The bioethanol for use as a liquid fuel by fermentation of renewable biomass as an alternative to petroleum is important from the viewpoint of global environmental protection. Recently, many scientists have attempted to increase the productivity of bioethanol process by developing specific microorganism as well as optimizing the process conditions. In the present study, which is based on our previous investigation on the pretreatment process, theproductivity of bioethanol obtained from simultaneous saccharification and fermentation (SSF) process was compared between various domestic materials including barley, brown rice, corn and sweet potato. Additionally, Solid glucoamylase (SGA; developed in Changhae Co.), from modified strain with UV, was used. The result was compared to commercial glucoamylase (GA). It was observed that the fermentation rate was increased together with the yield which can be derived from the final ethanol concentration. Especially, in the case of brown rice, compared to the experimental results using GA, the final ethanol concentration was 1.25 times higher and 18.4 g/L of the yield was increased. Also, the time required for reaching 95% of the maximum ethanol concentration is significantly reduced, which is approximately 36 hours, compared to 88 hours using GA. It means that SGA has excellent saccharogenic power.

Decreasing Effect of Kochujang on Body Weight and Lipid Levels of Adipose Tissues and Serum in Rats Fed a High-Fat Diet (고지방식이를 섭취한 흰쥐에서 고추장의 체중 및 지방조직과 혈청내의 지질감소효과)

  • 이숙희;공규리;정근옥;박건영
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.6
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    • pp.882-886
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    • 2003
  • Effect of kochujang (Korean red pepper soybean paste) on the reduction of body weight and lipid levels of adipose tissues and serum were investigated in the rats fed a high-fat diet. Male Sprague-Dawley rats were fed for four weeks with diets containing either a normal diet (ND, based on the AIN-76TM diet), high fat diet (HFD, supplemented with 20% corn oil in ND), CK diet (10% commercial kochujang in HFD), TK I diet (10% 0 day (non-) fermented traditional kochujang in HFD), TKII diet (10% 6-month fermented traditional kochujang in HFD), or RPP diet (3% red pepper powder in HFD). CK, TK I and TKII in the diet significantly reduced the body weight gain compared to the control (HFD), whereas red pepper powder had no such effect. TKII supplementation in the high-fat diet reduced epididymal and perirenal fat pad weight significantly (p<0.05). Liver total lipid was lower in TKII diet group than other kochujang and RPP diet groups. TKII and CK decreased the content of triglyceride in epididymal and perirenal fat pad that increased by high-fat diet. The cholesterol content of the perirenal fat pad was the lowest in the TKII diet group. TKII was also effective in lowering serum cholesterol level. These results indicated that properly ripened (fermented for 6-month) traditional kochujang exhibited more suppressive effects on body fat gain and lipid levels of adipose tissue and serum than RPP, commercial kochujang and the kochujang without fermentation.

Effects of Supplemental Vegetable Oils on Ruminal Characteristics, Digestibility and Formation of Fatty Acid Soaps in Sheep (식물성유 첨가가 면양의 반추의 발효특성과 소화율 및 지방산염 형성에 미치는 영향)

  • Kim, D.I.;Choi, J.R.;Cho, S.H.;Lee, D.H.;Chung, T.Y.
    • Journal of Animal Science and Technology
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    • v.46 no.5
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    • pp.783-798
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    • 2004
  • This experiment were conducted to determine the formation of fatty acid soaps, digestibility and ruminal characteristics when vegetable oils were added. Three Corriedale${\times}$ 3 Latin square design. Sheep were fed one of three diets consisting of alfalfa hay and concentrates in a ratio of 70:30. Dietary treatments were 1) no oil, 2) 7% soybean oil and 3) 7% corn oil. At the end of each experimental period, rumen contents from each sheep were collected before feeding and at 1, 3, 6 and 9h after feeding. pH decreased up to the 3 hour after feeding and increased until the end of the collection(p<0.0001). Whereas the concentration of $NH_3$-N showed inverse changes to pH(p<0.0001). Type of oil did not affect the molar concentration of total VFA, acetate, propionate and butyrate. Acetic/propionic acid (A/P) ratio decreased with increasing time(P<0.0001), but the ratio at the end of the experiment became similar to the initial value. No effects were found in the digestibility of dry matter, total-N, crude fiber, ash, NFE, NDF and ADF. The formation of fatty acid soaps(FAS) increased after feeding than 1 hour before feeding(P<0.05) and increased in 7% oils than no oil addition(P<0.05). Long chain fatty acids formed mainly FAS. ince the formation of FAS might decrease negative effects on ruminal haracteristics and total tract digestibility.

Effect of the Acute Phase Response on the Performance and Superoxide Dismutase Activity in Broiler Chicks Fed on Dietary Krill Meal (사료 중 크릴 밀을 급여한 육계의 생산성과 SOD 활성에 미치는 급성기 반응의 영향)

  • Park, I.K.;Kim, J.H.;Im, J.T.;Koh , T.S.
    • Journal of Animal Science and Technology
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    • v.46 no.2
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    • pp.183-192
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    • 2004
  • Two experiments were conducted to examine the effects of the acute phase response on the performance and superoxide dismutase(SOD) activities in liver and erythrocyte of broiler chicks fed dietary krill meals A and B in experiment 1 and krill meal A in experiment 2. The experimental diets are basal diet based on yellow corn and soybean meal and diets substituted 2.0% of krill meal A or B with soybean meal of the basal diet, respectively. Day-old birds fed on the experimental diets and the acute phase response(immunological stress) was activated in the birds on 8-day of age by alternate day injection i.p. with 3 doses the Salmonella typhymurium lipopolysaccharide(LPS) in saline. The values during the acute phase response were compared with those controls injected with saline. The performance; daily gain, feed intake, and feed efficiency were different between dietary krill meal A and B in birds during the acute phase response and in the control. The acute phase response increased relative liver and spleen weights. Recovery of birds from the immunological stress was different between krill meals. Dietary krill meals increased activities of MnSOD and Cu/ZnSOD in erythrocyte cytosols during the actute phase response. Dietary krill meals did not affect the PHA-p response. The results indicated that the dietary krill meals may accentuate oxidative stress during the acute phase response.

Viscometric Properties of Waxy Starches (찰전분류의 이화학적 특성(점성)비교)

  • Kim, Hyong-Soo;Woo, Ja-Won;Yoon, Gae-Soon;Heu, Mun-Hue
    • Applied Biological Chemistry
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    • v.28 no.4
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    • pp.219-225
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    • 1985
  • The viscometric properties of six kinds of waxy starches of Olchal and Hankang(waxy rice), Chalborie (waxy barley), Yullmoo (waxy Job's tears), Chajoe (waxy foxtail millet), Chalsusu (waxy great millet) and Chalocsusu (waxy corn) were investigated. The increase in optical transmittance of 0.1% starch suspensions occurred at $55^{\circ}C$ for Hankang, $60^{\circ}C$ for Olchal, Chalborie, Chajoe and Yullmoo and $65^{\circ}C$ for Chalsusu and Chalocsusu, and further inrreased $70^{\circ}C$ for Hankang and Olchal, $75^{\circ}C$ for Chajoe, Chalsusu, Chalocsusu and Yullmoo and above $85^{\circ}C$ for chalborie. Amylogram on 5%, starch suspensions showed that gelatinization temperature and maximum peak height were 850 B.U. at $62^{\circ}C$ for Hankang, $65^{\circ}C$ and 980 B.U. for Olchae, $64^{\circ}C$ and 1,080 B.U. for Chalborie, and 1,410 B.U. at $69^{\circ}C$ for Yullmoo, and 1,280 B.U. at $71^{\circ}C$ for Chajoe, and 1,260 B.U. at $71^{\circ}C$for Chalsusu and 1,420 B.U. at $70^{\circ}C$ for Chalocsusu. Swelling power of starches was $38{\sim}55$, and Hankang, Olchal, Chajoe, Chalocsusu and Yullmoo starches had higher swelling power than Chalsusu and Chalborie starches. Intrinsic viscosity of them was $1.43{\sim}1.75$ and hardness of them highly relevant to the gelatinization properties.

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