• Title/Summary/Keyword: Coriolus versicolor

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Decay Resistance and Effectiveness of CCA Preservative against Decay on the 4 Imported Softwoods (수입침엽수(輸入針葉樹) 4수종(樹種)의 내후성(耐朽性) 및 CCA계(系) 목재방부제(木材防腐劑) 처리(處理)에 따른 방부효과(防腐效果))

  • Lee, Jong Shin;Kim, Young Sik;Han, Kie Sun
    • Korean Journal of Agricultural Science
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    • v.22 no.1
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    • pp.42-48
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    • 1995
  • With the aim to investigation of decay resistance and optimum concentration in chrome-copper-arsenic(CCA) preservative treatment on the imported softwoods from Siberia and North america, preservative absorption after CCA impregnation, weight losses and degradation patterns by decay fungi were examined. The density and latewood rate of Siberia softwoods(Spruce and Larch) were higher than those of North america softwoods(Douglas-fir and Western hemlock), resulting in the decrease of the CCA preservative absorption in the Siberia softwoods. In the case of untreated softwoods, decay resistance against Coriolus versicolor was lower than against Tyromyces palustris. For CCA treated softwoods, preservative effectiveness increased with increase in concentration of CCA solution. When treated with 0.7% CCA solution, efficiency value was more than 80 and 90 for C. versicolor and T. palustris, respectively. From this results, in the CCA preservative treatment for imported softwoods, it can be concluded that optimum concentration of CCA solution is approximately 0.7%. The absorption of CCA preservative distributed in the range of 3.8 and $5.5kg/m^3$. After exposure to testing fungi, in the untreated softwoods, bore holes formed in the cell walls and bordered pits, moreover, bordered pit canals enlarged by the fungi. However, 0.5% CCA treated softwoods was almost no deterioration in the cell walls and bordered pits due to decay.

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Degradation of Three Aromatic Dyes by White Rot Fungi and the Production of Ligninolytic Enzymes

  • Jayasinghe, Chandana;Imtiaj, Ahmed;Lee, Geon-Woo;Im, Kyung-Hoan;Hur, Hyun;Lee, Min-Woong;Yang, Hee-Sun;Lee, Tae-Soo
    • Mycobiology
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    • v.36 no.2
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    • pp.114-120
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    • 2008
  • This study was conducted to evaluate the degradation of aromatic dyes and the production of ligninolytic enzymes by 10 white rot fungi. The results of this study revealed that Pycnoporus cinnabarinus, Pleurotus pulmonarius, Ganoderma lucidum, Trametes suaveolens, Stereum ostrea and Fomes fomentarius have the ability to efficiently degrade congo red on solid media. However, malachite green inhibited the mycelial growth of these organisms. Therefore, they did not effectively decolorize malachite green on solid media. However, P. cinnabarinus and P. pulmonarius were able to effectively decolorize malachite green on solid media. T. suaveolens and F. rosea decolorized methylene blue more effectively than any of the other fungi evaluated in this study. In liquid culture, G. lucidum, P. cinnabarinus, Naematoloma fasciculare and Pycnoporus coccineus were found to have a greater ability to decolorize congo red. In addition, P. cinnabarinus, G lucidum and T. suaveolens decolorized methylene blue in liquid media more effectively than any of the other organisms evaluated in this study. Only F. fomentarius was able to decolorize malachite green in liquid media, and its ability to do so was limited. To investigate the production of ligninolytic enzymes in media containing aromatic compounds, fungi were cultured in naphthalene supple mented liquid media. P. coccineus, Coriolus versicolor and P. cinnabarinus were found to produce a large amount of laccase when grown in medium that contained napthalene.

Screening and Evaluating of Wood-Rotting Fungi for Lignin Degradation and Ligninolytic Enzyme Production (II) - Laccase Production by Lignin-Degrading Fungi - (리그닌분해(分解)와 리그닌분해효소(分解酵素) 생산(生産)을 위한 목재부후균(木材腐朽菌)의 선발(選拔)과 평가(評價) (II) - 리그닌분해균(分解菌)에 의한 laccase 생산(生産) -)

  • Jung, Hyeun-Chae;Park, Seur-Kee;Kim, Byeong-Soo;Park, Chong-Yawl
    • Journal of the Korean Wood Science and Technology
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    • v.24 no.4
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    • pp.74-81
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    • 1996
  • 리그닌분해능(分解能)이 높은 균주(菌株)로 선발(選拔)된 Coriolus versicolor-13 (CV-13), LKY-7 및 LKY-12세 균주(菌株)에 대하여 균체외(菌體外) laccase 생산(生産)을 검토(檢討)하였다. Glucose-peptone broth에서 균체외(菌體外) laccase활성(活性)은 CV-13의 경우 3일 이상배양후(以上培養後)에 나타났고 LKY-7과 LKY-12균주(菌株)의 laccase 활성(活性)은 배양(培養) 2일째에 검출(檢出)되었다. 탄소원(炭素源)으로서는 maltose가 glucose와 비슷한 laccase 생산효과(生産效果)를 나타냈고 질소원(窒素源)으로서는 유기태질소(有機態窒素)가 무기태(無機態) 질소(窒素)보다 효과적(效果的)이었다. Laccase 유도물질(誘導物質)로서는 2,5-Xylidine이 가장 우수하였으며 1mM 이하(以下)의 농도(濃度)에서는 유도효과(誘導效果)가 크게 나타났으나 1.5mM 이상(以上)의 농도(濃度)에서는 laccase생산(生産)이 억제(抑制)되었고, 균사생장(菌絲生長) 초기(初期)에 첨가(添加)하는 것이 효과적(效果的)으로 나타났다. SDS-PAGE 후, CV-13 균주(菌株)의 균체외(菌體外) 단백질(蛋白質)에서는 약 69, 66, 25, 23, 19kDa 크기의 laccase band가 5개 나타났고 LKY-7 균주(菌株)에서는 27kDa과 19kDa 크기의 2개 band가, LKY-12 균주(菌株)에서는 22, 20, 17kDa 크기의 laccase band가 3개 나타났다.

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Optimal Production and Characterization of Fibrinolytic Enzyme from Fomitella fraxinea Mycelia. (Fomitella fraxinea 균사체로부터 Fibrin분해효소의 최적생산 및 효소적 특성)

  • 이종석;백형석;박상신
    • Microbiology and Biotechnology Letters
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    • v.30 no.4
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    • pp.325-331
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    • 2002
  • investigated to maximize the production of fibrinolytic enzyme from Fomitella fraxinea mycelia. Among the tested media, Coriolus versicolor medium (CVM) showed the highest production for the enzyme. 2% galactose, 0.6% yeast extract and 0.1% $NaNO_3$, 0.1% $K_2HPO_4$, and 0.05% $MgSO_4$.$7H_2O$ as carbon, nitrogen, phosphorus, and inorganic salt sources resulted in the maximum level of the enzyme activity, respectively. The enzyme production from F. fraxinea was reached to highest level after the cultivation for 10 days at $25^{\circ}C$ and pH 9. The enzyme activity of culture supernatant was most active at $40^{\circ}C$ and pH 10. The activity of the enzyme was inhibited by phenylmethylsulfonylfluoride and aprotinin, suggesting that it is a serine protease.

Macrophage Stimulating Activity of Exo-Biopolymer from Submerged Culture of Lentinus edodes with Rice Bran

  • Yu, Kwang-Won;Shin, Kwang-Soon;Choi, Yang-Mun;Suh, Hyung-Joo
    • Journal of Microbiology and Biotechnology
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    • v.14 no.4
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    • pp.658-664
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    • 2004
  • To find a new utilization of rice bran, nine higher fungi were examined for the production of exo-biopolymer with macrophage stimulating activity from rice bran. Among the exo-biopolymers produced from submerged cultures, Lentinus edodes showed the highest activity, followed by Grifola frondosa, Schizophyllum commune, and Coriolus versicolor. L. edodes also had the most potent macrophage stimulating activity in a liquid culture rather than in a solid culture. In order to improve rice bran utilization and the yield of exo-biopolymer with macrophage stimulating activity, the treatment of Rapidase effectively increased the macrophage stimulating activity (about 30% increase), whereas the other enzymes (Econase, Viscozyme, Ultraflo, Celluclast, and Thermylase) treatments did not increase the macrophage stimulating activity. Exo-biopolymer with macrophage stimulating activity from L. edodes contained mainly neutral sugars (58.7%) with considerable amounts of uronic acid (32.2%) and a small amount of proteins (9.1%). Component sugars of exo-biopolymer consisted of mainly arabinose, galactose, glucose, mannose, and xylose (0.95:0.81:0.96:1.00:0.39, respectively). When the exo-biopolymer was treated with $NaIO_4, NaClO_2$, and pronase, the $NaClO_2$ treatment and pronase digestion had little effect, whereas $NaIO_4$ oxidation significantly decreased the macrophage stimulating activity (47.6% reduction at $100\mug/ml$). Therefore, the carbohydrate moiety in exo-biopolymer from L. edodes plays an important role in the expression of the macrophage stimulating activity.

Screening of inhibitory effect of 40 herbs on platelet aggregation induced by ADP (40종(種) 한약재(韓藥材)의 adenosine diphosphate에 의한 혈소판(血小板) 응집(凝集) 저해작용(沮害作用) 검색(檢索))

  • Cho, Young-Joo;Kim, Sung-Hoon
    • Journal of Haehwa Medicine
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    • v.5 no.1
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    • pp.185-198
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    • 1996
  • After evaluation of antithrombotic effect of 40 herbs on platelet aggregation induced by ADP(Adenosine diphosphate), these results were obtained as follows: 1. Crude drugs exerting over 30 % of in Chinemys reevesii (Gray)hibition on platelet aggregation induced by ADP were Ganoderma japonicum (Fr.) Lloyd., Panax ginseng C. A. Mey., Gastrodia elata Bl., Thea sinensis, Chinemys reevesii (Gray), Cuscuta chinensis Lam., Cervus nippon Temminck., Biota orientalis (L.) Endl., Coriolus versicolor, Cinnamomum cassia Presl., Sophora flavescens Ait., Amomum villosum Lour., Carthamus tinctorius L., Rubus chingii Hu., Poria cocos (Schw.) Wolf., Laminana japonica Aresch., Ligustrum lucidum Ait., Angelica sineusis (Oliv.), Cyperus rotundas L., Ginkgo biloba L., Zingiber officinale Rosc., Prunus persica (L.) Batsch., Schizandra chinensis (Turcz.) Baill. and Plantago asiatica L.. 2. Of crude drugs having showed over 50% of inhibitory effect on platelet aggregation, at the concentration of $100{\mu}g/m{\ell}$, the inhibitory rates were 82.2% in Ganoderma japonicum (Fr.) Lloyd., 55% in Panax ginseng C. A. Mey., 50.8% in Gastrodia elata Bl., while at the concentration of $200{\mu}g/m{\ell}$, antithrombotic rates were 89.4% in Ganoderma japonicum (Fr.) Lloyd., 59.2% in Panax ginseng C. A. Mey., 57.9% in Thea sinensis, 52.7% in Gastrodia elata Bl.. These results suggest that the study sholuld be necessary on antithrombotic effect of solvent fractions of Ganoderma japonicum (Fr.) Lloyd., Panax ginseng C. A. Mey., Gastrodia elaha B1. and Thea sinensis and isolation of effective compound from above drugs.

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Antiturmor Activities of Protein-bound Polysaccharide Extracted from Mycelia of Mushroom (버섯 균사체에 의한 암세포 성장억제 효과)

  • 권석형;김춘년;김철용;권석태;박기문;황보식
    • The Korean Journal of Food And Nutrition
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    • v.16 no.1
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    • pp.15-21
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    • 2003
  • The purpose of this study is to observe the effect of protein-bound polysaccharide (PBP) on proliferation of Th1 cells and cytotoxicity of cancer cell. Mushrooms (Ganoderma lucidum, Agaricus blazei, Lentinus edodes, Coriolus versicolor and Phellinus linteus) were fractionated by 100$^{\circ}C$ hot water for 3hr. PBP was stimulated and proliferated Th1 cells most at 10 mg/$m\ell$ concentration and the percentage of cell proliferation was 40%. It was estimated cytotoxicity of PBP against 7 kinds of cancer cell lines. Antitumor activities of Agaricus blazei against P388D1 and L1210 (tumor cell lines) were 2.4% and 39.7% survival rate, and Lentinus edodes was 48.4% and 52.5% survival rate, respectively. PBP mixtures of Agaricus and Lentinus edodes prolonged (27∼40%) significantly the survival rate of mice intraperitoneally implanted with sarcoma 180.

Comparison of Antibacterial and Antioxidant Activities of Mushroom Mycelium Culture Extracts Cultivated in the Citrus Extracts (감귤농축액 첨가배지에서 배양한 버섯균사체 추출물의 항균활성 및 항산화활성 비교)

  • Kim Man-Chul;Kim Min-Joo;Kim Taeg;Park Guen-Tae;Son Hong-Joo;Kim Gi-Young;Choi Woo-Bong;Oh Duck-Chul;Heo Moon-Soo
    • KSBB Journal
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    • v.21 no.1 s.96
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    • pp.72-78
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    • 2006
  • This study was carried out to investigate the antimicrobial and antioxidative effects of mycelium cultural extract from mushroom. Mushroom mycelium was grown in a defined synthetic liquid medium and citrus extracts, and the culture extracts were examined for antioxidant and antibacterial activity. Myceliums of Phellinus linteus, Cordyceps militaris, Coriolus versicolor, Sparassic crispa, Agaricus blazei, lnonotus obliquus, Lentinus edodes, Hericium erinacium, Gonoderma lucidium in 10% citrus extract supplemented medium and synthesis medium were incubated in a shaking incubator (120 rpm, $24{\sim}30^{\circ}C$ ) for $7{\sim}15$ days. The antimicrobial activity of the culture fluid of mushroom mycelium grown in submerged liquid culture was tested against 12 microorganisms which were fish pathogens and common bacterial species. The culture extracts showed high activity against Vibrio sp. and had poor effect on Streptocouus sp., S. parauberis, S. iniae. The culture extracts obtained from the synthetic medium showed $30{\sim}93%$ of the 1,1-diphenyl-2-picrylhydrazyl radical scavenger activity, the culture extracts obtained from the citrus extracts medium exhibited antioxidant activity up to 55%.

Anti-oxidative Activities of Commercial Edible Plant Extracts Distributed in Korea (국내 유통 중인 식용식물 추출물의 항산화효과)

  • Kim, Kyung-Bum;Yoo, Ki-Hwan;Park, Ha-Yan;Jeong, Jong-Moon
    • Applied Biological Chemistry
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    • v.49 no.4
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    • pp.328-333
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    • 2006
  • Many plant extracts are known to have antioxidative effects. However, their activities can be reduced or disappeared during mass production process. The purpose of this study is to compare antioxidative effects of edible plant extracts distributed in Korea. forty three kinds of edible plant extracts commercially available in Korea were selected and investigated for their total phenolics contents and antioxidative potentials(DPPH radical and superoxide anion radical scavenging activities). In contents of total phenolics, the commercial plant extracts from Artemisia annua(whole plant), Ilex paraguariensis(leaf, Silybum marianum(fruit and leaf, Ulmus pumila(bark), Coliolus versicolor(fruit), and Curcuma longa(root and stem) contained over 70 mg/g of powder, DPPH radical scavenging activities($SC_{50}$, 50% scavenging concentration) of A. annua, I. paraguariensis, Pinus densiflora(leaf),S. marianum, U. pumila, and C. longa were $53.96{\pm}0.81\;ppm,\;24.61{\pm}2.12\;ppm,\;35.96{\pm}1.11\;ppm,\;57.46{\pm}2.13\;ppm,\;55.25{\pm}1.65\;ppm\;and\;12.99{\pm}1.67ppm$, respectively, while that of positive control(vitamin C) was $3.86{\pm}0.81\;ppm$. $SC_{50}$ values against superoxide anion radical of A. annua, Cinnamomum zeylanicum(bark), I. paraguariensis, Rubus coreanus(fruit and leaf), Morus alba(leaf), P. densiflora, S. marianum, U. pumila, C. versicolor, C. longa, Perilla frutescens var. acuta(leaf), and H. sabdariffa(leaf and newer) were $53.21{\pm}1.83ppm,\;50.12{\pm}2.12ppm,\;5.59{\pm}0.84ppm,\;41.60{\pm}8.93ppm,\;20.19{\pm}0.97ppm,\;15.19{\pm}1.66ppm,\;21.20{\pm}1.88ppm,\;15.71{\pm}0.91ppm,\;55.48{\pm}2.42ppm,\;52.12{\pm}2.44ppm,\;23.80{\pm}1.98ppm\;and\;11.14{\pm}0.51ppm$, respectively($SC_{50}$ value of vitamin C: $9.61{\pm}0.93ppm$). In particular, both 1 paraguariensis and P. densiflora had high content of phenolics as well as high scavenging activities of DPPH radical and superoxide anion radical. Consequently, above two commercial extracts may be useful as a source of antioxidative nutraceutics.

Effect of PS-K on nile tilapia, Oreochromis niloticus, as an adjuvant and prophylactic agent (나일 틸라피아에 대한 질병예방제 및 면역보조제로서의 PS-K 효과 분석)

  • Park, Kyun-Hyun;Ha, Jai-Yi;Huh, Min-Do;Huh, Sung-Hoi;Jeong, Hyun-Do
    • Journal of fish pathology
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    • v.10 no.1
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    • pp.45-52
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    • 1997
  • A protein-bound polysaccharide preparation, PS-K, isolated from Coriolus versicolor was evaluated for its ability to enhance resistance against Edwardsiella tarda, causal agent of classical edwardsiellosis in tilapia, Oreochromis niloticus. Fish treated with a dose rate of 0.1 mg PS-K $g^{-1}$ body weight were challenged by intraperitoneal injection of E. tarda with different concentrations. Against low burdens of injections($1.2{\times}10^7$ and $1.2{\times}10^8$ cfu/fish), PS-K treated fish did not show any mortality compared to 50% and 100% mortality of control groups, respectively. However there was no increase of resistance against challenge with high concentrations of E. tarda ($1.2{\times}10^9$ and $1.2{\times}10^{10}$cfu /fish) except few days delaying of death. Tilapia injected with PS-K one day after intraperitoneal inoculation of E tarda($1.2{\times}10^7$ cfu/fish) showed 100% survival rate compared to control group of 50% survival rate. The result indicates the potential of PS-K as a prophylactic agent in aquaculture. The increased antibody response in fish received PS-K one week prior to FKC administration suggested the influence of PS-K on the specific humoral immunity and increased resistance against bacterial infection.

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