• Title/Summary/Keyword: Copepod culture

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The Culture of free-swiming Copepod Secies Apocyclops sp. (Copepod; Cyclopoida)by baking Yeast (빵 이스트를 먹이로 이용한 부유성 코페포다 Apocyclops sp.(Copepod: Cyclopoida)의 배양)

  • Jung, Min-Min;Kim, Hyeung-Sin;Rho, Sum;Rumengan, Innke F.M.;Hagiwara, Atsushi
    • Journal of Aquaculture
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    • v.12 no.4
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    • pp.303-307
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    • 1999
  • Copepod is useful live food organism in the larval rearing. However of fin fish, culcures of copepod species (Specially calanoida and cyclopoida) were very difficult under artificial management conditions. In this study, we report successful culture of a free swimming cyclopodia copepod species (Apocyclops sp.) by easily baker's yeast as food. The maximum culture density of Apocyclops sp. was observed 11,200±2,660 ind/ℓ during the 30 days culture periods in the 1.5ℓ culture tanks. The nauplii were growth up maximum density 6,400±432 ind/ℓ on day 16th. The number of egg carring female was observed maximum density (666.7 ind./ℓ) of two times after 6days and 28 days of initial culture.

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Survival Strategies of the Rotifer Brachionus rotundiformis for Coexisting with the Copepod Apocyclops borneoensis in Laboratory Culture

  • Jung, Min-Min
    • Fisheries and Aquatic Sciences
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    • v.15 no.1
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    • pp.57-62
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    • 2012
  • Interspecific relationship between a euryhaline rotifer Brachionus rotundiformis and a cyclopoid copepod Apocyclops borneoensis was investigated in the laboratory culture. In a mixed culture of B. rotundiformis and A. borneoensis, population growth of B. rotundiformis was suppressed from day 10, while growth in a monoculture population continuously increased throughout the experimental period. However, the population growth of A. borneoensis in the mixed culture did not markedly differ from that in a monoculture population. Suppression of B. rotundiformis growth coincided with a decrease in the numbers of both non-egg-bearing and egg-bearing females, and increasing resting egg formation. Growth of A. borneoensis was not affected by the presence of the rotifer. However, relative growth index of ovisac bearing females in the mixed culture was 1.62 times higher than that in the monoculture. Presence of the copepod did not greatly reduce the food available to the rotifer population. The rotifer B. rotundiformis responded in a unique way, to stresses such as physical damage (filtering by A. borneoensis) with the production of many resting eggs to increase its chances of survival.

Mass culture of the brackish water cyclopoid copepod Paracyclopina nana Smirnov (기수산 요각류 Paracyclopina nana의 대량배양)

  • Lee, Kyun-Woo;Choi, Young-Ung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.8
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    • pp.262-266
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    • 2016
  • Marine copepods are ideal live prey for fish larvae, and many studies on the mass culture of the organism have been reported. This study performed a mass culture of the brackish copepod Paracyclopina nana containing nauplius and C4-adult production methods. In nauplius production, the harvested nauplii over 95% were comprised of N1 and N2. Daily mean nauplius production of two trials for 15 days were $6.9{\times}10^6$ and $7.2{\times}10^6$ individuals, respectively. The densities of the adult females were maintained at a similar level of the initiation during production. In C4-adult production, the proportion of harvested copepods containing C4-adult males, females and ovigerus females were 49%, 28%, and 18%, respectively. The daily mean nauplius production of the two trials for 16 days were $8.2{\times}10^5$ and $9.0{\times}10^5$ individuals, respectively. As a result, the continuous production of P. nana using the mass production system was successful. Therefore, the continuous and stable feeding for fish larvae in aquaculture would be possible by the selection of the copepod culture method depending on the mouth size of the fish.

Effects of Temperature and Salinity on Productivity and Growth of Five Copepod Species (수온과 염분에 따른 5종 요각류의 성장과 생산력)

  • LEE Kyun Woo;PARK Heum Gi
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.38 no.1
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    • pp.12-19
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    • 2005
  • This study investigated the productivity and growth of copepods, Sinocalanus tenellus 2 strains, Paracyclopina nana 3 strains, Apocyclops royi 2 strains, Tachidius triangularis 1 strain and Tigriopus sp. 1 strain at different temperatures $(24,\;28\;and\;32^{\circ}C)$ under 15 psu and various salinities (10, 20, 30 psu) at $28^{\circ}C$ for an individual culture. And the mass culture was carried out to evaluate the production of these copepods in the 500 mL vessel. For an individual culture of copepod, survival period of a brood female tended to increase with the decrease of temperature, but was not significantly influenced by salinity. With an increase of temperature and a decrease of salinity, maturation period of nauplii in all species tended to decrease. Total nauglii production from a broodo female at $24^{\circ}C$ was higoher than that from a brood .female at either 28 or $32^{\circ}C$ for S. tenellus and at $32^{\circ}C$ than either 24 or $32^{\circ}C$ for A. royi Haenam stram, but was not influenced by temperature for another copepods. And total nauplii production from a brood female at 10 and 20 psu tended to be higher than that from a brood female at 30 psu, expect for Tigriopus sp. and P. nana Hwajinpo strain. Total nauplii production from a brood female was the highest in Tigriopus sp. (350 nauplii) at $28^{\circ}C$ under 20 psu, followed by A. royi Haenam strain (187 nauplii) at $32^{\circ}C$ under 15 psu and P. nana Hwajinpo strain (152 nauplii) at $24^{\circ}C$ under 10 psu. In the mass culture of each copepod, P. nana was the most productive (26.6 mg dry weight) and this value was two and four times higher than productivity of Tigriopus sp. and A. royi, respectively.

Interspecific relationship between two food organisms in the combination culture tank of rotifer, Brachionus rotundiformis and copepod, Tigriopus japonicus (로티퍼, Brachionus rotundiformis와 코페포다, Tigsiopus japonicus의 혼합 배양조에 있어서 두 종간의 상호 관계)

  • JUNG Min-Min;RHO Sum;KIM Hyeung-Sin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.1
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    • pp.66-69
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    • 2000
  • The common harpacticoida copepod species Tigriopus japnnicus was often obseued in the mass culture of marine rotifer tanks of sea farming stations. In this study, we investigated the co-existing effect of T. iaponicus to the rotifer Brachionus rotundiformis population growth. The culture conditions as temperature, salinity, culture volume, photo period, culture period and observation interval were 25{\circ}C, 22ppt, 40ml, 12L:12D, 16 deys$ and every two days during the experimental period, respectively. Nannochloropsis oculata was used as the food for the two testing organisms. After counting the growth of rotifer and copepod, they were transferred to new culture tank with N. oculata at the density of $7{times}10^5\;cells/ml$. The population growth of B. rotundiformis was suppressed by T. japonicus when these two organisms were mix-cultured comparing to single culture of B. rotundiformis. But population growth of T, japomcus was not affected by the presence of B. rotundiformis. The interspecific relationships of prey-predation and food competition were not observed in the combination culture of rotifer B. rotundiformis and copepod T japonicus.

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Effect of Light Intensity on Survival, Growth and Productivity of the Cyclopoid Copepod Paracyclopina nana: A Laboratory Study (Paracyclopina nana (Crustacea, Cyclopoida)의 생존, 개체발생 및 생산력에 관한 조도의 영향: 실험실내 배양)

  • Lee, Kyun-Woo;Kang, Jung-Hoon;Park, Heum-Gi
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.6
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    • pp.671-676
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    • 2011
  • To determine the optimum light intensity for mass culture of the brackish-water cyclopoid copepod Paracyclopina nana, survival, growth, and productivity of the copepod were examined at several light intensities (0, 10, 100, 500, 1,000 lx). The survival rate of P. nana from nauplius to adult decreased with increasing light intensity. The highest survival rate was found under the dark condition, with 61.7% surviving; no significant difference was observed between 0 and 10 lx (51.7%) and the lowest survival rate was with 100 lx (26.7%). Survival rates at 500 and 1,000 lx were significantly lower in comparison with other conditions. The developmental period from nauplius to copepodid (5.8 days) and to adult (11.8 days) at 10 lx was significantly shorter than in the other treatments. Daily mean nauplius production of adult females over 7 days at 0, 10 and 100 lx was significantly higher than at 500 and 1,000 lx. In the 1,000 lx treatment, 99% of the adult females died on the $14^{th}$ day. The optimum light intensity for the mass culture of P. nana could be 10 lx, which had no adverse effects on survival, development, or reproduction.

Observation of Co-Existing Copepods in the Marine Rotifer Mass Culture Tanks (해산 로티퍼 대량 배양 수조에서 관찰되는 코페포다의 혼재 양상)

  • Jung, Min-Min;Kim, Hyowon;Hur, Jun Wook;Kim, Jung;Kang, Kyoung Ho
    • Journal of Marine Bioscience and Biotechnology
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    • v.9 no.2
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    • pp.30-34
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    • 2017
  • Microorganisms are contained filtered sea water that is why we often observed co-existing copepod species in the large-scale rotifer culture tanks. In this study, we investigated about taxonomical study of co-existing copepods in Brachionus rotifer mass culture tanks of several seafarming centers at Jeju-do and Pusan, Korea by microscope. Also, we counted number of copeods and density in 10 mL of cultured sea water and distinguished growing patterns and sex. As results, we can identified 4 species copepods, 2 species of Tisbe, 1 species of Tigriopus and 1 species of Apocyclops.

Evalution of Three Species of Haptophyte Algae for the Culture of the Marine Copepod Tigriopus japonicus (해산 요각류 Tigriopus japonicus 개체군 성장에 미치는 착편모조의 영향)

  • Kim, Hyeung-Sin;Jung, Min-Min
    • Journal of Aquaculture
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    • v.18 no.2
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    • pp.86-91
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    • 2005
  • Three haptophyte algae, Isochrysis galbana, Prymnesium parvum and P. patelliferum were offered to the marine copepod Tigriopus japonicus as food. Growth rate of larvae, egg production, mortality and comsumption rates of T. japonicus were measured for each of the haptophyte species offered. The growth rate of larvae, egg production and algal ingestion of T. japonicus fed on P. parvum and P. patelliferum were much lower than those fed on I. galbana and corresponding high mortality rates were also observed during the experimental period. The harmful effects observed during the present study indicate that bloom-forming haptophyte algae, P. parvum and P. patelliferum are not suitable feed species for culture of copepod, T. japonicus.

Culturing Method and Dietary Value of Benthic Copepod, Tigriopus japonicus (부착성 요각류, Tigriopus japonicus의 배양방법 및 먹이효율)

  • 박흠기;허성범;김철원
    • Journal of Aquaculture
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    • v.11 no.2
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    • pp.261-269
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    • 1998
  • The harpacticoid copepod, Tigriopus japonicus is one of the important zooplankton as a live food the production of marine fish fry. This reserch was carried out to investigate the culturing method and dietary value of T. japonicus. The reproduction of this copepod was analysed in the culturing systems (tne opened and the closed systems) and the surface area of the substate plates for the mass culture. Survival rate of the mature female and the number of nauplii produced per female for two day was higher in the open culturing system than in the close system. However, the result of survival rate of the nauplius was reversed. Larger surface area of the culturing vessel accommodated higher density of this bentic copepod. In polyculture of T. japonicus and rotifer (B. plicatilis), growth of T. japonicus seems to be dependent on the rotifer density. But rotifer seems to be independent on the copepod. With regard to dietary value, this copepod is better than Artemia nauplius for the larvae of red sea bream and tiger puffer which feed by picking. However, it is inadequate for bottom fish larvae as flounder.

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Parturition and Early Growth of Crowned Seahorse, Hippocampus coronatus in Korea (한국산 해마, Hippocampus coronatus의 출산과 초기성장)

  • Choi Young-Ung;Rho Sum;Jung Min-Min;Lee Young-Don;Noh Gyoung-Ane
    • Journal of Aquaculture
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    • v.19 no.2
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    • pp.109-118
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    • 2006
  • The recent decrease in wild stocks of seahorse, Hippocampus coronatus, has prompted the production of the species in captivity. We here present data on the body color changes of the species according to culture conditions. This investigation examined relationship between adult body size and clutch and growth in 60 days after parturition and survival of juveniles with four fed enriched Artemia nauplii, copepod nauplii and copepod adult. Seahorse adults ($77.77{\pm}9.84mm$, n=12) collected from Kamak Bay and Yeoja Bay from August 2003 to November 2004 gave a parturition of $6\sim75$ juveniles (Mean, 39 juveniles/male/time) for 14 times. A day old seahorses were $14.48{\pm}1.38mm$ (n=15) in standard length (SL) with 14 dorsal fin rays, 12 pectoral fin rays and 4 anal fin rays. Sixty-day old seahorses were $24.65{\pm}0.83mm$ (n=4) in SL. Growth rate of seahorses was 0.18 mm/day at 24 in 60 days after parturition. When offered Artemia nauplii and copepod nauplii, 5 day old sea-horses preferred copepod nauplii to Aremia nauplii with a maximum predation rate of 31 copepod nauplii/sea-horse/h. However 30-day old seahorses preferred selected Artemia nauplii with a maximum predation rate of 14 Artemia nauplii/seahorse/h. Survival was highest (49%) when offered copepod nauplii together with enriched Artemia nauplii.