• Journal of Applied Biological Chemistry
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• v.59 no.3
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• pp.207-213
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• 2016

Little information about the biological activities of Citrus limon (lemon) leaves has been reported, whereas the fruit of Citrus limon (lemon) has been well-documented to contain various pro-health bio-functional compounds. In the present study, the antiproliferative activities of the lemon leaves were evaluated using several cancer cell lines. From the n-hexane, chloroform, ethyl acetate, n-butanol, and water fractions of methanolic extract of the leaves, the chloroform fraction of lemon leaves (CFLL) showed the most potent antiproliferative activity in the AGS human gastric cancer cells. The current study demonstrates that CFLL induces apoptosis in AGS cells, as evidenced by an increase in apoptotic bodies, cell population in the sub-G1 phase, Bax/Bcl-2 ratio, and cleavage of poly (ADP-ribose) polymerase (PARP), caspase-3 and caspase-9. Compositional analysis of the CFLL using gas chromatography mass spectrometry (GC-MS) resulted in the identification of 27 compounds including trans, trans-farnesol (3.19 %), farnesol (3.26 %), vanillic acid (1.45 %), (-)-loliolide (5.24 %) and palmitic acid (6.96 %). Understanding the modes of action of these compounds individually and/or synergistically would provide useful information about their applications in cancer prevention and therapy.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.16 no.6
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• pp.1-6
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• 2017

Recently, the brittle materials such as ceramics, glass, sapphire and textile material have been widely used in semiconductors, aerospace and automobile owing to high functional characteristics. On the other hand, it has the characteristics of difficult-to-cut material relative to all materials. In this study, diamond electro-deposited band-saw machine was developed to operate stably using water-coolant type through relative motion between band-saw tool and $Al_2O_3$ material. High densified $Al_2O_3$ material was manufactured by spark plasma sintering method. The bulk density was observed by the Archimedes law and the theoretical density was estimated to be $3.88g/cm^3$ and its hardness 14.7 MPa. From the dicing sawing test of $Al_2O_3$ specimen, behavior of surface roughness and band-saw wear are dominantly affected by the increase of the band-saw linear velocity. Additionally, an continuous pattern type of diamond band-saw was a very effective due to entry impact as a one-off for brittle material.

• Journal of Physiology & Pathology in Korean Medicine
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• v.35 no.3
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• pp.97-103
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• 2021

Perilla frutescens (P. frutescens) is an important herb used for many purposes such as medicinal, aromatic, and functional food in Asian countries and has beneficial effects such as antioxidant activity, anti-inflammation activity, anti-depression activity, and anxiolytic activity. However, there have been no studies on the protective effect of P. frutescens extract (PFE) on amnesia in vivo. The present study aimed to investigate whether PFE protects memory deficit using a scopolamine-induced mice model and elucidate the underlying mechanisms involved. The protective effect of PFE against scopolamine-induced memory deficits was investigated using Y-maze, passive avoidance, and Morris water maze tests. Furthermore, the potential mechanisms of PFE in improving memory capabilities related to the cholinergic system and antioxidant activity were examined. PFE significantly increased spontaneous alternation in the Y-maze test, step-through latency in the passive avoidance test, and swimming time in the target quadrant in the probe test when compared to the scopolamine-treated group. Likewise, PFE significantly decreased escapes latency in the Morris water maze test. PFE could not regulate cholinergic function in acetylcholine level and acetylcholine esterase activity. However, PFE increased DPPH radical scavenging activity dose-dependently and total polyphenol content was 127.7±1.2 ㎍ GAE/mg. The results showed that the PFE could be a preventive and/or therapeutic candidate for memory and cognitive dysfunction in Alzheimer's disease.

• The Journal of the Convergence on Culture Technology
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• v.10 no.2
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• pp.73-80
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• 2024

To determinate the content of acanthoic acid and kaurenoic acid in 70% EtOH and hot water extracts of Eleutherococcus gracilistylus, quantitative analysis of each compound in samples was carried out by a HPLC-UVD. Also, the identification of each acompound in samples was successfully assigned by LC-MS analysis. In result, the contents of acanthoic acid and kaurenoic acid in 70% ethanoic extracts were 28.84±0.21 mg/g (2.88%), 26.38±1.63 mg/g (2.64%), respectively. However, the content of two compounds in hot-water extracts was not observed. In conclusion, it shows that 70% ethanol as a best extraction solvent to extract the acanthoic acid and its metabolite from Eleutherococcus gracilistylus was better than hot-water solvent. The 70% ethanol complex extract of Allium Hookeri and Eleutherococcus gracilistylus showed better effectiveness. In addition, the 70% ethanol extract complex of Allium Hookeri and Eleutherococcus gracilistylus showed better effects than the hot water solvent of DPPH radical scavenging ability, total polyphenols, and flavonoids content. The anti-inflammatory activity were significantly or partially reduced by treatment with ethanol extract complex(SEC) by Allium Hookeri and Eleutherococcus gracilistylus.

• Journal of Digital Convergence
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• v.16 no.7
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• pp.89-96
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• 2018

In this paper, we proposed a parametric estimation method for estimating H/W cost by using the development data of professional service robot in Korea. In addition, we derived the factors and weights that we can estimate the costs depending on the application environmental conditions of the robot. For the analysis, we developed the equation of professional service robot cost estimation using parametric method. We also derived the adjustment factors and following weights through FGI and Delphi for environmental conditions. We have developed a cost estimation equation that reflects the weight, volume, and manufacturing difficulty, and can derive a relational equation that reflects the environmental factors(dust/water, heat/cold, safety, test, technology innovation). This provides an objective basis for estimating the cost of professional service robots and will lead to ongoing research for estimating the H/W development cost of professional service robots. In the future, we will increase reliability by collecting abundant data, and will strengthen models through finding functional factors.

• Food Science and Preservation
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• v.24 no.6
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• pp.834-841
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• 2017

This study was carried out to investigate the anti-oxidative and anti-inflammatory effects of hot water extracts of Ligularia fischeri cultivated in Youngyanggun. We obtained hot water extract (HWE) and cold water extract (CWE) from L. fischeri. The anti-oxidative activities of L. fischeri extracts were measured by 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity. The anti-inflammatory effects of L. fischeri were evaluated in human mast cell line-1 (HMC-1) cells stimulated with phorbol-12-myristate-13-acetate plus A23187 (PMACI). The solid yields of HWE was 150% higher than CWE solid yield. Total polyphenol contents of HWE were $198.07{\pm}0.24mg/g$. The value of anti-oxidative activities of HWE were shown $IC_{50}$ $28.2{\pm}0.04ug/mL$. We showed that HWE significantly reduced the PMACI-induced the production of IL-6 (0.01-1 mg/mL), IL-8 (0.1-1 mg/mL), and $TNF-{\alpha}$ (0.01-1 mg/mL). These results indicate that the HWE of L. fischeri can be used as a functional material due to its antioxidant and anti-inflammatory activities.

• Journal of the Korea Organic Resources Recycling Association
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• v.31 no.2
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• pp.63-71
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• 2023

Increased nitrogen in the water system has become an important environmental problem around the world, as it causes eutrophication, algae bloom, and red tide, destroys the water system, and undermines water's self-purification. The most common form of nitrogen in the water system is ammonium ion (NH₄⁺), and the largest portion of ammonium ions comes from wastewater. NH₄⁺ is a major contributor to eutrophication, which calls for appropriate treatment and measures for ammonium removal. This study produced biochar by applying Sorghum × drummondii, a type of biomass with a great growth profile, analyzed the adsorption capacity of Sorghum × drummondii biochar produced from the changing carbonization temperature condition of 200 to 400℃ in the ammonium ion range of 10 to 100 ppm, and used the results to evaluate its potential as an adsorbent. Carbonization decomposed the chemical structure of Sorghum × drummondii and increased the content of carbon and fixed carbon in the biochar. The biochar's pH and electrical conductivity showed high adsorption potential for cations due to electrical conductivity as its pH and electrical conductivity increased along with higher carbonization temperature. Based on the results of an adsorption experiment, the biochar showed 54.5% and 17.4% in the maximum and minimum NH₄-N removal efficiency as the concentration of NH₄-N increased, and higher carbonization temperature facilitated the adsorption of pollutants due to the biochar's increased pores and specific surface area and subsequently improved NH₄-N removal efficiency. FT-IR analysis showed that the overall surface functional groups decreased due to high temperature from carbonization.

• Journal of Life Science
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• v.33 no.5
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• pp.383-390
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• 2023

The goal of this study was to identify new bioactive peptides in extracts derived from Tenebrio molitor (T. molitor) larvae for the development of functional foods. After extraction from freeze-dried T. molitor larvae with various solvents on time course, the extracts showed the highest 2,2,1-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity at 5 and 10 hr per total protein and solid contents, respectively. When the water extract was fractionated, a high methanol concentration led to a reduced level of high-molecular-weight proteins in the centrifugal supernatant, whereas increased DPPH activity in the supernatants suggests low-molecular-weight peptides may mediate antioxidant activity in the supernatant. Most of the organic solvent partitions, excluding butanol, showed similar activities in the water phases, and the organic solvent partition fraction exhibited a 28~44% decrease in activity following heat treatment, implying that some components in the fraction become unstable in the presence of heat. The addition of proteinase K to the water extract increased DPPH activity by 10~20%, suggesting that peptides, when released from total proteins, partially increase antioxidant activity. Therefore, we suggest that the antioxidants in T. molitor larval extracts make them a potential source of functional animal food.

• Food Science of Animal Resources
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• v.37 no.5
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• pp.743-751
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• 2017

During slaughtering, animal blood is typically discarded, resulting in water pollution. However, this discarded blood has valuable components, such as immunoglobulin (Ig). Although several studies have been conducted to develop methods for effective recycling of slaughterhouse blood, they have not been commercially utilized in Korea. Here, we extracted an Ig-rich fraction from porcine blood that was then subjected to various in vitro tests, including pathogen growth inhibition, antigenic cross-reactivity, and anti-toxin activity. The porcine immunoglobulin concentrate (PIC) was effectively purified by eliminating other components, such as albumin, and consisted of approximately $63.2{\pm}2.9%$ IgG and $7.2{\pm}0.4%$ IgM on a protein basis. The results showed that it significantly suppressed the growth of pathogenic bacteria, and bound to all tested pathogens, including both gram-positive and gram-negative species, although the degree of activity differed according to strain. The PIC bound to two types of lipopolysaccharide (LPS) obtained from Escherichia coli O111:B4 and Salmonella enterica serotype typhimurium in a concentration-dependent manner. In addition, the PIC restored the proliferation activity of the lymphoblast K-562 cells when co-incubated with pathogenic LPS. These results confirm that the PIC prepared in this study is a potentially valuable functional food material or diet supplement as an alternative to antibiotics that can protect animals from pathogenic bacteria.

• Atmosphere
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• v.20 no.2
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• pp.131-151
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• 2010

This study establishes a conceptual model to analyze heavy rainfall events in Korea using multi-functional transport satellite-1R satellite images. Three heavy rainfall episodes in two major synoptic types, such as synoptic low (SL) type and synoptic flow convergence (SC) type, are analyzed through a conceptual model procedure which proceeds on two steps: 1) conveyer belt model analysis to detect convective area, and 2) cloud top temperature analysis from black body temperature (TBB) data to distinguish convective cloud from stratiform cloud, and eventually estimate heavy rainfall area and intensity. Major synoptic patterns causing heavy rainfall are Changma, synoptic low approach, upper level low in the SL type, and upper level low, indirect effect of typhoon, convergence of tropical air in the SC type. The relationship between rainfall and TBBs in overall well resolved areas of heavy rainfall. The SC type tended to underestimate the intensity of heavy rainfall, but the analysis with the use of water vapor channel has improved the performance. The conceptual model improved a concrete utilization of images and data of satellite, as summarizing characteristics of major synoptic type causing heavy rainfall and composing an algorism to assess the area and intensity of heavy rainfall. The further assessment with various cases is required for the operational use.