• 제목/요약/키워드: Conventional screening system

검색결과 53건 처리시간 0.027초

PC 클러스터 기반의 Multi-HPSO를 이용한 안전도 제약의 경제급전 (The Security Constrained Economic Dispatch with Line Flow Constraints using the Multi PSO Algorithm Based on the PC Cluster System)

  • 장세환;김진호;박종배;박준호
    • 전기학회논문지
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    • 제58권9호
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    • pp.1658-1666
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    • 2009
  • This paper proposes an approach of Mult_HPSO based on the PC cluster system to reduce or remove the stagnation on an early convergence effect of PSO, reduce an execution time and improve a search ability on an optimal solution. Hybrid PSO(HPSO) is combines the PSO(Particle Swarm Optimization) with the mutation of conventional GA(Genetic Algorithm). The conventional PSO has operated a search process in a single swarm. However, Multi_PSO operates a search process through multiple swarms, which increments diversity of expected solutions and reduces the execution time. Multiple Swarms are composed of unsynchronized PC clusters. We apply to SCED(security constrained economic dispatch) problem, a nonlinear optimization problem, which considers line flow constraints and N-1 line contingency constraints. To consider N-1 line contingency in power system, we have chosen critical line contingency through a process of Screening and Selection based on PI(performace Index). We have applied to IEEE 118 bus system for verifying a usefulness of the proposed approaches.

Green Fluorescent Protein-reporter Mammalian One-hybrid System for Identifying Novel Transcriptional Modulators for Human $p14^{ARF}$ Tumor Suppressor Gene

  • Lee, Hye Jin;Yang, Dong Hwa;Yim, Tae Hee;Rhee, Byung Kirl;Kim, Jung-Wook;Lee, Jungwoon;Gim, Jin Bae;Kim, JungHo
    • Animal cells and systems
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    • 제6권4호
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    • pp.317-322
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    • 2002
  • To improve conventional yeast one-hybrid screening, we have developed an efficient mammalian one-hybrid system that allows rapid isolation of com-plementary DNAs which are able to induce human p14$^{ARF}$. tumor suppressor gene. A 1.5 kb promoter region of p14$^{ARF}$ was fused to EGFP to generate ARF promoter-EGFP reporter vector. This reporter plasmid was stably trans-fected into NIH3T3 cells for generation of reporter cell line. When the reporter cell line was infected with E2F-1 together with excess amounts of empty vector, the cells that received the positive modulator were readily identifiable by green fluorescence using FACS. The GFP-positive cells were cloned directly from the cultured cells and expanded in bulk culture. The genomic DNAs from GFP-positive cells were prepared and the CDNA insert in integrated retroviral genome was recovered by PCR using primers annealing to the retroviral vector sequences flanking the insert-cloning site. This system should be useful for efficient screening of expression CDNA libraries in mammalian cells to identify novel upstream regulators for spe-cific genes by one-hybrid interaction.ion.

Integrated Quantitative Phosphoproteomics and Cell-Based Functional Screening Reveals Specific Pathological Cardiac Hypertrophy-Related Phosphorylation Sites

  • Kwon, Hye Kyeong;Choi, Hyunwoo;Park, Sung-Gyoo;Park, Woo Jin;Kim, Do Han;Park, Zee-Yong
    • Molecules and Cells
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    • 제44권7호
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    • pp.500-516
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    • 2021
  • Cardiac hypertrophic signaling cascades resulting in heart failure diseases are mediated by protein phosphorylation. Recent developments in mass spectrometry-based phosphoproteomics have led to the identification of thousands of differentially phosphorylated proteins and their phosphorylation sites. However, functional studies of these differentially phosphorylated proteins have not been conducted in a large-scale or high-throughput manner due to a lack of methods capable of revealing the functional relevance of each phosphorylation site. In this study, an integrated approach combining quantitative phosphoproteomics and cell-based functional screening using phosphorylation competition peptides was developed. A pathological cardiac hypertrophy model, junctate-1 transgenic mice and control mice, were analyzed using label-free quantitative phosphoproteomics to identify differentially phosphorylated proteins and sites. A cell-based functional assay system measuring hypertrophic cell growth of neonatal rat ventricle cardiomyocytes (NRVMs) following phenylephrine treatment was applied, and changes in phosphorylation of individual differentially phosphorylated sites were induced by incorporation of phosphorylation competition peptides conjugated with cell-penetrating peptides. Cell-based functional screening against 18 selected phosphorylation sites identified three phosphorylation sites (Ser-98, Ser-179 of Ldb3, and Ser-1146 of palladin) displaying near-complete inhibition of cardiac hypertrophic growth of NRVMs. Changes in phosphorylation levels of Ser-98 and Ser-179 in Ldb3 were further confirmed in NRVMs and other pathological/physiological hypertrophy models, including transverse aortic constriction and swimming models, using site-specific phospho-antibodies. Our integrated approach can be used to identify functionally important phosphorylation sites among differentially phosphorylated sites, and unlike conventional approaches, it is easily applicable for large-scale and/or high-throughput analyses.

An Efficient and Stable Method for the Transformation of Heterogeneous Genes into Cephalosporium acremonium Mediated by Agrobacterium tumefaciens

  • XU WEI;ZHU CHUNBAO;ZHU BAOQUAN
    • Journal of Microbiology and Biotechnology
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    • 제15권4호
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    • pp.683-688
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    • 2005
  • A transformation system mediated by Agrobacterium tumefaciens is routinely used for the genetic engineering of plants. Here, we report an efficient and stable method for transformation of heterogeneous genes into an industrial Cephalosporium acremonium by using a similar transformation system established in plants. Both the phleomycin-resistant gene and vgb gene were used as screening markers to confirm the success of transformation by either Southern hybridization or PCR amplification. It was found that acetosyringone (AS) was necessary only for protoplast transformation and the heterogeneous genes transferred were integrated into the genome of C. acremonium. The transformation efficiency obtained with this system was much higher than the conventional techniques used for transformation of C. acremonium.

Combinatorial Solid Phase Peptide Synthesis and Bioassays

  • Shin, Dong-Sik;Kim, Do-Hyun;Chung, Woo-Jae;Lee, Yoon-Sik
    • BMB Reports
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    • 제38권5호
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    • pp.517-525
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    • 2005
  • Solid phase peptide synthesis method, which was introduced by Merrifield in 1963, has spawned the concept of combinatorial chemistry. In this review, we summarize the present technologies of solid phase peptide synthesis (SPPS) that are related to combinatorial chemistry. The conventional methods of peptide library synthesis on polymer support are parallel synthesis, split and mix synthesis and reagent mixture synthesis. Combining surface chemistry with the recent technology of microelectronic semiconductor fabrication system, the peptide microarray synthesis methods on a planar solid support are developed, which leads to spatially addressable peptide library. There are two kinds of peptide microarray synthesis methodologies: pre-synthesized peptide immobilization onto a glass or membrane substrate and in situ peptide synthesis by a photolithography or the SPOT method. This review also discusses the application of peptide libraries for high-throughput bioassays, for example, peptide ligand screening for antibody or cell signaling, enzyme substrate and inhibitor screening as well as other applications.

하이브리드 알고리즘을 응용하여 안전도제약을 만족시키는 최적전력조류 (Security Constrained Optimal Power Flow by Hybrid Algorithms)

  • 김규호;이상봉;이재규;유석구
    • 대한전기학회논문지:전력기술부문A
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    • 제49권6호
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    • pp.305-311
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    • 2000
  • This paper presents a hybrid algorithm for solving optimal power flow(OPF) in order to enhance a systems capability to cope with outages, which is based on combined application of evolutionary computation and local search method. The efficient algorithm combining main advantages of two methods is as follows : Firstly, evolutionary computation is used to perform global exploitation among a population. This gives a good initial point of conventional method. Then, local methods are used to perform local exploitation. The hybrid approach often outperforms either method operating alone and reduces the total computation time. The objective function of the security constrained OPF is the minimization of generation fuel costs and real power losses. The resulting optimal operating point has to be feasible after outages such as any single line outage(respect of voltage magnitude, reactive power generation and power flow limits). In OPF considering security, the outages are selected by contingency ranking method(contingency screening model). The OPF considering security, the outages are selected by contingency ranking method(contingency screening model). The method proposed is applied to IEEE 30 buses system to show its effectiveness.

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Analysis of Kernel Hardness of Korean Wheat Cultivars

  • Hong, Byung-Hee;Park, Chul-Soo
    • 한국작물학회지
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    • 제44권1호
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    • pp.78-85
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    • 1999
  • To investigate kernel hardness, a compression test which is widely used to measure the hardness of individual kernels as a physical testing method was made simultaneously with the measurement of friabilin (15KDa) which is strongly associated with kernel hardness and was recently developed as a biochemical marker for evaluating kernel hardness in 79 Korean wheat varieties and experimental lines. With the scattered diagram based on the principal component analysis from the parameters of the compression test, 79 Korean wheat varieties were classified into three groups based on the principal component analysis. Since conventional methods required large amount of flour samples for analysis of friabilin due to the relatively small amount of friabilin in wheat kernels, those methods had limitations for quality prediction in wheat breeding programs. An extraction of friabilin from the starch of a single kernel through cesium chloride gradient centrifugation was successful in this experiment. Among 79 Korean wheat varieties and experimental lines 50 lines (63.3%) exhibited a friabilin band and 29 lines (36.7%) did not show a friabilin band. In this study, lines that contained high maximum force and the lower ratio of minimum force to maximum force showed the absence of the friabilin band. Identification of friabilin, which is the product of a major gene, could be applied in the screening procedures of kernel hardness. The single kernel analysis system for friabilin was found to be an easy, simple and effective screening method for early generation materials in a wheat breeding program for quality improvement.

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In-Vitro Anticancer and Free Radical Scavenging Potential of Compound Formulation Used in Unani System of Medicine

  • Mannan, Mohd Nazir;Kazmi, Munawwar Husain;Chakraborty, Alokananda;Zakir, Mohammad;Ahmad, Tasleem;Lahari, K.
    • 셀메드
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    • 제10권4호
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    • pp.27.1-27.6
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    • 2020
  • Cancer is one of the leading cause of mortality in India as well as worldwide. The management of cancer by conventional therapy has shown life threatening adverse effects. The researchers are now exploring the natural way of treatment. Unani system of medicine have rich literature for cancer and many compound formulations have been described in this system. Unani system of medicine is based on holistic approach and treat human being as a unit with natural herbs, mineral and animal origin drugs. An important compound Unani formulation (CUF) from the literature has been chosen to explore the Unani claim of its anticancer activity. The phytochemical constituents were assessed using standard phytochemical screening method. Antioxidant property of this formulation was assessed by DPPH assay. The DPPH free radical scavenging assay was carried out by colorimetric method and ascorbic acid was taken as a positive control. Three different extracts of CUF on different concentrations were used to screening on human breast cancer (BCC) MCF-7 cell line. For the estimation of in-vitro cytotoxic potency of the investigated extracts was assessed on MTT assay by using trypan blue method and paclitaxel was used as the standard. Hydro-ethanolic (HE) extract showed highest free radical scavenging activity among all extracts. DPPH Assay showed substantial antioxidant activity of these extracts in hydro-ethanol extract at 1㎍ concentration of CUF. The CUF showed antioxidant and anticancer activity. The claim made by Unani physician has been proved.

광선의 차폐가 없는 광시야 비축 2반사광학계 설계 (Optical Design of a Wide-field Off-axis Two-mirror System without Ray Obstruction)

  • 오혜진;이종웅
    • 한국광학회지
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    • 제28권6호
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    • pp.263-272
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    • 2017
  • 넓은 시야를 가지는 광학계에서는 전반부는 음의 굴절능, 후반부는 양의 굴절능을 가지는 inverted telephoto 구조가 일반적으로 사용되고 있고, 2반사광학계에서는 inverse Cassegrain system이 inverted telephoto 구조에 해당한다. 하지만 통상적인 회전대칭 구성의 inverse Cassegrain system에서는 시야가 가려지고 광선의 차폐가 심한 문제점이 있다. 이 연구에서는 이점을 고려하여 inverse Cassegrain system의 제2면에 조리개를 두어 시야를 넓히고, 광선의 차폐가 없는 비축시야만을 사용하는 광시야 비축 2반사광학계를 설계하였다.

의료분쟁 해결제도의 개혁-미국 및 일본의 경험을 중심으로- (The Proposal of Reforming for Resolving Medical Malpractice Disputes)

  • 이규식
    • 보건행정학회지
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    • 제1권1호
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    • pp.72-94
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    • 1991
  • The number of disputes between physicians and patients caused by medical malpractice are showing a sharp increasing over the past several years. The disputes on medical malpractice may be resolved either in court or by direct negotiation between both sides concerned. There are no special acts relating to the civil or penal liability of the physicians in Korea. The medical disputes are decided merely through legal technicalities and without reference to actual medical practice. The current system which does not compensate injured patients adequately or equitably leads to taking a long time consuming for dispute resolution processes. The things make worsed, the problem is due to not being of insurance system or a proper funds for compensation. This research proposes a outline of new and comprehensive alternative for these problems and failure of conventional resolution of medical disputes. So far, we have learned lessons from the excperiencies of resolving medical malpractice disputes of Japan and the United States. The proposal first calls for an administrative arbitration and pretrial screening panels as a condition precedent to trial. The proposal also includes to facilitate with the funds for compensating the injured.

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