• Korean Journal of Agricultural Science
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• v.20 no.1
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• pp.34-42
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• 1993

Effects of salt concentrations on the cell growth and the pigment accumulation were investigated in cell suspension culture of Vitis vinifera and Phytolacca americana L.. The growth pattern of vine cell in control was showed the normal exponential growth pattern, but in the dilution media delay the exponential growth pattern from 4 to 8 days after culture. Maximal accumulation of anthocyanin was observed at 12 days after culture in all treatments. In cell suspension culture of Phytolacca, accumulation of betacyanin occurred in parallel with the cell growth pattern and maximal accumulation of betacyanin was observed after 8 days of culture. In the vine cell culture, the cell growth was showed the peak at 87.6mM of sucrose in the medium and reduced at over this concentration. Maximal anthocyanin accumulation was showed at 146mM of sucrose. In the higher concentrations of sucrose, the cell growth was rapidly decreased, but the accumulation of anthocyanin was not. Otherwise, in case of Phytolacca cell culture, betacyanin accumulation was showed in parallel with the cell growth increased with sucrose concentration. It was suggested that the anthocyanin of vine and the betacyanin of Phytolacca were controlled by different mechanisms.

• Korean Journal of Agricultural Science
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• v.27 no.2
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• pp.86-94
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• 2000

This study was carried out to determine the effectiveness of uniconazole in ameliorating low and high temperature injury in tomato plants(Lycopersicon esculentum Mill. cvs. Fireball and Patio). Plants were given a soil drench of 0, 0.001, 0.01 or $0.1mg{\cdot}pot^{-1}$ uniconazole, and after 14 days, were treated with 12-h day/12-h night cycles at $25/25^{\circ}C$, $2.5/25^{\circ}C$, $25/2.5^{\circ}C$ or $40/40^{\circ}C$ for 4 days in controlled-environment chamber. Number of damaged leaves per plant, reduction of stem elongation, and overall injury were high at $2.5/25^{\circ}C$, but more reduction of leaf elongation, delay of flowering, and abortion of floral bud were observed in plants at $40/40^{\circ}C$. There was difference in degree of injury between cultivars, thus, 'Fireball' was much affected by unfavorable temperature regimes. All concentrations of uniconazole reduced leaf and stem elongation, increased total chlorophyll concentration, delayed flowering, and significantly provided protection against low and high temperature injury in two cultivars. In general, the application of uniconazole did not inhibit flowering delay and floral bud abortion induced by high and low temperature exposure. Our results support the hypothesis that the role of uniconazole is related to defense system against oxidative stress induced by low temperature stress. Further research is required to clarifu the phytoprotective mechanism of this compound agaist high temperature stress.

• Korean Journal of Agricultural Science
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• v.38 no.4
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• pp.631-639
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• 2011

This research was conducted to investigate potential use of New Zealand spinach (Tetragonia tetragonoides) as a new vegetable crop which will be cultivating in salt-affected soils including reclaimed land. Traditionally New Zealand spinach has been studied to explore functional compound or salt removing potential. To cultivate the crop species in the salt-affected soil widely, it is essential to obtain salt and soil texture responses under the controlled environment. Fifty nine New Zealand spinach ecotypes native to Korean peninsula first collected over seashore areas, and primitive habitat soil environment was evaluated by analyzing soil chemical properties from 32 locations. Different textures of sandy, silt loam, and sandy loam soils were prepared from nearby sources of sea shore, upland and paddy soils, respectively. Target salinity levels of 16.0 dS/m, 27.5 dS/m, 39.9 dS/m, and 52.4 dS/m in electrical conductivity (ECw) were achieved by diluting of 25, 50, 75, 100% (v/v) sea water to tap water (control, 0.6 dS/m), respectively. Various measurements responding to soil texture and irrigation salinity included plant height, root length, fresh weight (FW), dry weight (DW), leaf parameters (leaf number, leaf length, leaf width), lateral branching, and inorganic ion content. was found to adapt to diverse habitats ranging various soil chemical properties including soil pH, organic matter, exchangeable bases, EC, and cation exchange capacity (CEC) in Korea. Responding to soil texture, New Zealand spinach grew better in silt loam and sandy loam soil than in sandy soil. Higher yield (FW and DW) seemed to be associated with branch number (r=0.99 and 0.99, respectively), followed by plant height (r=0.94 and 0.97, respectively) and leaf number (r=0.89 and 0.84, respectively). Plant height, FW, and DW of the New Zealand spinach accessions were decreased with increasing irrigation salinity, while root length was not significantly different compared to control. Based on previous report, more narrow spectrum of salinity range (up to 16 dS/m) needs to be further studied in order to obtain more accurate salinity responses of the plant. As expected, leaf Na content was increased significantly with increasing salinity, while K and Ca contents decreased. Growth responses to soil texture and irrigation salinity implied the potential use of New Zealand spinach as a leafy vegetable in salt-affected soil constructed with silt loam or sandy loam soils.

• Journal of Bio-Environment Control
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• v.27 no.4
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• pp.415-423
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• 2018

The aim of this study was to evaluate the individual glucosinolate (GSL), total phenol, total flavonoid, and vitamin C content, and antioxidant activity under various light quality condition, mainly focusing on red (R) to far-red (FR) light ratios in three Brassica sprouts (radish, Chinese cabbage, and broccoli). Three R/FR ratio of 0.6, 1.3, and 2.0 were exposed to 5-day old sprouts for 48 h in a controlled environment, and the targeted phytochemical contents and antioxidant activities were compared with three separate control plot of dark, fluorescent, and red:blue 8:2 conditions. Total GSL content was highest in broccoli among the cultivars throughout the respective treatments, and increased with the increasing of R/FR ratio in the broccoli sprouts, while the content showed non-significant results in the Chinese cabbage sprouts. The progoitrin, a major GSL in Chinese Cabbage and broccoli, content decreased by upto 38% and 69%, respectively, with decreasing the R/FR ratio compared to the control plots (fluorescent, red:blue 8:2, and dark condition). The contents of phenol, flavonoid, and vitamin C were lowest in dark condition in all the three Brassica sprouts. The total phenol content and antioxidant activities increased with decreasing the R/FR ratio in all the Brassica sprouts, while total flavonoid and vitamin C content showed different patterns depending upon the Brassica sprouts. These results suggest that additional use of FR is expected to improve the functional quality of Brassica sprouts in different ways.

• Korean Journal of Agricultural Science
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• v.10 no.1
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• pp.74-83
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• 1983

In order to examine the relationship of fruit abscission and ethylene evolution in 'Jojuro' and 'Imamuraaki' pear trees, the thinning chemicals, ${\beta}$-naphthalene acetic acid (NAA), O, O-dimethyl-O-(3-methyl-4 nitrophenyl) posphorothioate (MEP), 1-naphthyl N-methyl carbamate (carbaryl), and 2-chloroethyl phosphonic acid (ethephon) were applied. Concurrently the effect of calcium acetate on the control of fruit abscission were examined when calcium acetate was added to ethephon and carbaryl. 1. All the applied chemicals (MEP, NAA, carbaryl and ethephon) was effective to abscise fruits in 'Jojuro', and MEP and carbaryl in 'Imamuraaki's. 2. The application of ethephon increased the ethylene evolution but other chemicals did not increase it. 3. There were no significant differences in total sugar contents of fruits by MEP, NAA, carbaryl and ethephon treatments. 4. When the calcium acetate was added to the ethephon and carbaryl, the thinning effects were offsetted. 5. The rate of defoliation due to ethephon treatment was controlled by addition of calcium acetate at 0.1~0.25 M. It was clarified that fruit abscission induced by thinning chemicals except ethephon was not directly related to ethylene production and that degree of fruit thinning can be regulated by addition of calcium acetate to thinning chemicals.

• Horticultural Science & Technology
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• v.34 no.3
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• pp.424-432
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• 2016

This experiment was carried out to compare growth and fruit setting characteristics in ten winter-planted paprika (Capsicim annuum L.) cultivars. Five red line paprika cultivars ('Maduro', 'Maranello', 'Nagano', 'Sirocco' and 'Special') and five yellow line paprika cultivars ('Coletti', 'Sven', 'Thialf', 'Volante' and 'Zagato') were used for this study. The experiment was performed for 42 weeks, from winter of 2013 to autumn of 2014. Based on the growth stages of paprika, growth characteristics were investigated six times, and each investigation was categorized from group 1 through group 6. The relative internode ratio showed a normal range at the early growth stage, but tended to gradually decline as growth progressed. This trend was greater in red line paprika than in yellow line paprika. Among the cultivars used for this experiment, 'Special' and 'Zagato' showed growth inhibition, whereas 'Maranello' and 'Volante' kept a balanced growth during summer cultivation. The fruit set percentage in yellow paprika was 10.8% higher than in red paprika, while the number of branches in yellow paprika was 4.1% less than in red paprika. When measured after the full fruiting age, from June to July, the number of fruit set in group 4 was much lower in 'Sirocco' and 'Coletti' than in any of the other cultivars, indicating that they were more sensitive to the growth environment during the rainy season. These findings suggest that 'Maranello', 'Nagano', 'Sven', 'Thialf', and 'Volante' could be effectively used for summer cultivation in a high-plastic-film greenhouse, and 'Maranello', 'Special', 'Volante', and 'Zagato' could be good candidates for a low-plastic-film greenhouse.

• Journal of Bio-Environment Control
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• v.30 no.3
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• pp.175-182
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• 2021

This experiment was conducted to find out the possibility of use of reused rockwool and comparison of growth, productivity and quality of tomatoes according to the use of rockwool and coir medium. The experiment was conducted in an automatic controlled greenhouse at Andong National University, College of Life Science, located in Andong, Gyeongsangbuk-do.. As a result of the experiment, there was no difference in the number of leaves, plant height, and leaf area between treatments, and the crown diameter was slightly higher in rockwool medium, also there was no difference between reused rockwool and coir medium. Fruit productivity showed different responses depending on the cultivation environment, but there was no significant difference between rockwool, reused rockwool and coir medium. In addition, the quality of fruit was observed to be different according to the concentration of EC in the medium. Therefore, in tomato hydroponic cultivation, there was no difference in the type of medium in growth, productivity, fruit quality and the environmental and water management had a great effect, and it is expected that the reuse of rockwool will have a positive effect on the economic point of view.

• Horticultural Science & Technology
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• v.34 no.2
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• pp.294-304
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• 2016

Members of the genus Brassica, which are known as oil crops or cruciferous vegetables, are widely cultivated in Canada, Australia, Asian and Europe. Because Brassica species have high yields, are well adapted to their environments, and are self-incompatible, the germplasm is abundant. Previous studies have reported abundant genetic diversity even within Brassica subspecies. In Korea, fresh cabbage leaves are eaten with roast meat, and to meet the current popular demand, new varieties are being increasingly bred. To determine the genetic diversity and relationships among the cabbage vegetables in Korea, we evaluated the genetic variation of 18 accessions based on 5S and 18S ribosomal RNA (rRNA) gene sequences. We detected many variable nucleotide sites, especially in the 5S rRNA gene sequences. Because the length of the 18S rRNA gene might influence the dissimilarity rate statistics, we used both the 5S and 18S sequences to analyze the phylogenetic relationships. S7 (B. oleracea) showed the most distant phylogenetic relationship with the other Brassica species. Interestingly, B2 (B. oleracea), B15, and B18 (B. campestris) have three different types of leaf profiles, and were divided into one group, and the other Brassica species formed another group. Statistical analysis of interspecies and intraspecies genetic distances revealed that B. campestris L. showed higher genetic diversity than B. oleracea L. This work provides additional data that facilitates the evaluation of the genetic variation and relationships among Brassica species. The results could be used in functional plant breeding programs to improve Brassica crops.

• Research in Community and Public Health Nursing
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• v.14 no.2
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• pp.287-298
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• 2003

The purpose of the study was to develop an integrated prevention program to strengthen elders self-care capability and to examine its effectiveness on their psychological condition. This study used one group pre- and post-test design. Subjects were 85 elderly residents (over 65 years of age) who lived alone, and received free basic medical care and social welfare services in a rural community in Korea. Subject eligibility criteria for this study were to an elders who 1) is not currently taking any anti-depressant medication 2) is able to communicate, and 3) agrees to participate in this study. The integrated program was composed of horticulture, reminiscence, and friendship activities. Twelve sessions were provided for 12 weeks in community-based partnerships to achieve better outcomes. The intervention was case-managed by a public health nurse and aided by six volunteers. The main outcome variable was depression, which was assessed by using 15 items selected from the Geriatric Depression Scale-short form Korean version. Socio-demographic characteristics, functional status, and satisfaction with social support were used as covariates. Results showed that there was a significant intervention effect at post-intervention time point compared to pre-intervention time point(E.S. 0.94). Multiple linear regression analysis showed significant interaction effects between intervention and satisfaction with social support. These findings must be interpreted within the context that an effects of an integrated program could be more synergistically increased when social support factor is considered in the program. A community-based integrated prevention program of depression is effective for vulnerable rural elderly. It is suggested that randomized controlled trials within community setting for better methodological strength as well as multi-level outcomes on community need to be conducted in future.

• Journal of Microbiology and Biotechnology
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• v.26 no.9
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• pp.1542-1550
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• 2016

This is the first report that paromomycin, an antibiotic derived from Streptomyces sp. AG-P 1441 (AG-P 1441), controlled Phytophthora blight and soft rot diseases caused by Phytophthora capsici and Pectobacterium carotovorum, respectively, in chili pepper (Capsicum annum L.). Chili pepper plants treated with paromomycin by foliar spray or soil drenching 7 days prior to inoculation with P. capsici zoospores showed significant (p < 0.05) reduction in disease severity (%) when compared with untreated control plants. The disease severity of Phytophthora blight was recorded as 8% and 50% for foliar spray and soil drench, respectively, at 1.0 ppm of paromomycin, compared with untreated control, where disease severity was 83% and 100% by foliar spray and soil drench, respectively. A greater reduction of soft rot lesion areas per leaf disk was observed in treated plants using paromomycin (1.0 μg/ml) by infiltration or soil drench in comparison with untreated control plants. Paromomycin treatment did not negatively affect the growth of chili pepper. Furthermore, the treatment slightly promoted growth; this growth was supported by increased chlorophyll content in paromomycin-treated chili pepper plants. Additionally, paromomycin likely induced resistance as confirmed by the expression of pathogenesis-related (PR) genes: PR-1, β-1,3-glucanase, chitinase, PR-4, peroxidase, and PR-10, which enhanced plant defense against P. capsici in chili pepper. This finding indicates that AG-P 1441 plays a role in pathogen resistance upon the activation of defense genes, by secretion of the plant resistance elicitor, paromomycin.