• The Plant Pathology Journal
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• v.31 no.2
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• pp.123-131
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• 2015

Clavibacter michiganensis subsp. sepedonicus (Cms) multiplies very rapidly, passing through the vascular strands and into the stems and petioles of a diseased potato. Therefore, the rapid and specific detection of this pathogen is highly important for the effective control of the pathogen. Although several PCR assays have been developed for detection, they cannot afford specific detection of Cms. Therefore, in this study, a computational genome analysis was performed to compare the sequenced genomes of the C. michiganensis subspecies and to identify an appropriate gene for the development of a subspecies-specific PCR primer set (Cms89F/R). The specificity of the primer set based on the putative phage-related protein was evaluated using genomic DNA from seven isolates of Cms and 27 other reference strains. The Cms89F/R primer set was more specific and sensitive than the existing assays in detecting Cms in in vitro using Cms cells and its genomic DNA. This assay was also able to detect at least $1.47{\times}10^2copies/{\mu}l$ of cloned-amplified target DNA, 5 fg of DNA using genomic DNA or $10^{-6}$ dilution point of 0.12 at $OD_{600}$ units of cells per reaction using a calibrated cell suspension.

• Magazine of the Korean Society of Agricultural Engineers
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• v.17 no.1
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• pp.3702-3711
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• 1975

This study was originated to investigate the imparired parts of the mist and dust blowers and intended to analyze the causes of their failures by the use of the Weibull probability paper. By the use of the paper, the parts which were needed to change the design, the force of the urgency, the mean time between failures of the parts and the basic causes of the troubles could be predicted. The survey showed that the following parts got out of order: (A) flexible rubber hose, (B) blowing fan, (C) lead valve, (D) piston ring. (E) crank main bearing, (F) coil in magneto and (G) needle valve in carburettor. The analysis of the survey indicated that the parts G, C, E and D were belonged to "wear-out failure", and that the mean time between failures became shorter in order as indicated above. To insure longer lives of those parts, it would be necessary to change the design and the material and to upgrade operators mechanical technique of the mist and dust blower. The failure of the parts A, B and F was classified as "randomfailure", and they did not seem to be the "wear-out" at that time. The parts B and F was evaluated as "initial failure". Quality controls and operating tests by the producer. and the operational training for users should be strengthened so as to eliminate these failures. Were the failures only in the parts A, C, D, E, F and G, it could be said that the "reliability" of the mist and dust blower would fall to 10 percent in the second year, and 50 percent of the mist and dust blowers would fail in all parts A, C, D, E, F and G before the blower would have control insect and disease in 58.2 hectares. The use of the Weibull probability paper for analyzing failures of the mist and dust blowers was effective in that it analyzed failure in the relationship between strength of the parts and users actual circumstanoes.

• Korean Journal of Veterinary Research
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• v.39 no.2
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• pp.318-326
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• 1999

Respiratory pathogenic effects of several porcine reproductive and respiratory syndrome virus(PRRSV) isolates were examined in swine tracheal ring(STR) cultures by examining their effect on ciliary activity. One high and one low pathogenic PRRSV isolates were then selected and their pathogenicity investigated in 3-week-old conventional PRRSV-seronegative pigs. Ten pigs each were inoculated intranasally with the high or low pathogenic PRRSV isolate and 6 pigs were sham inoculated as negative controls. Two pigs each from the inoculated group and one pig each from negative control group were killed on 4, 7, 14, 21 and 28 days postinoculation(pI). At necropsy, degrees of gross lung lesion was determined. Turbinate, tonsil, trachea and lung samples were collected for virus isolation or histopathology. Gross lung lesions were observed mainly on 14 days PI with high and low pathogenic isolates inducing moderate diffuse and mild gross lung lesions, respectively. Inoculation of either the high or low pathogenic virus resulted in loss of cilia in ciliated epithelium of turbinates and trachea between 7 and 28 days PI. High pathogenic virus caused increased number of Goblet cells in the tracheal epithelial layer between 4 and 21 days PI whereas the low pathogenic virus did it between 14 and 28 days PI and with a lesser degree. Although both viruses produced interstitial pneumonia, the lesion was less severe with the low pathogenic virus. The isolation of high pathogenic virus from tissues and sera was earlier and more consistent than that of the low pathogenic virus. The agreement between in vitro and in vivo tests indicates that STR cultures may be used as a routine method to determine the respiratory pathogenicity of PRRSV isolates.

• Journal of the Korean Wood Science and Technology
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• v.16 no.1
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• pp.9-20
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• 1988

Lespedeza species have been widely used not only as plant resources for bark, leaves and honey, but also as erosion control materials. This study was carried out to investigate the structural and wood tiber characteristics in order to obtain basic information for the utilization of the wood. The wood structure was investigated for 10 selected species and the wood fiber, for the 5 selected species among Lespedeza species distributed all over the country. The following results were obtained. In the cross section, campylotropis showed ray diffuse porous wood, L. bicolor, L. cyrtobotrya, L. thunbergii var. intermedia, diffuse porous wood, and L. tomentella, L. angustifolioides. L. maritima, L. robusta, L. patentibicolor, ring porous wood. The maximum diameter of a single vessel ranged 66-123 ${\mu}$ in all species. Campylotropis showed the most number of vessels, L. angustifolioides, the least. The number of ray per mm ranged 7-22, Campylotropis showed the most number of rays, L. angustifolioides, the least. In the radial section the average length of vessel ranged 121-250 ${\mu}$ in all species. L. thunbergii var. intermedia showed the longest vessel, L. tomentella, the shortest. In tangential section the average width of the uniseriate ray ranged 9.2-14.7${\mu}$, that of the multiseriate ray, 19.2-42.1 ${\mu}$. The average height of the uniseriate ray ranged 143.0-1162.0 ${\mu}$. The width of fiber ranged 10.12-13.61 ${\mu}$, L. maximowiezii showed the narrowest tiber, L. thunbergii var. intermedia. the widest, the thickness of fiber wall ranged 2.93-3.71 ${\mu}$ in the five species. L. maximowiezii showed the most thin fiber wall, L. thunbergii var. intermedia, the thickest, L. cyrtobotrya showed the difference in the size of fiber between the shade and the sunny sites but L. maximowiezii showed no difference. There was significant difference in fiber length, fiber width and wall thickness between L. eyrtobotrya and L. maximowiezii.

• Membrane Journal
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• v.30 no.3
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• pp.200-204
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• 2020

1,3,5-benzenetricarbonyl trichloride is a chemical substance in which three acyl chlorides are located at 1,3,5 position in the benzene ring, and is an important chemical for the area where the good physical and chemical properties are required through high degree of crosslinking. In particular, it is possible to form a three-dimensional structure having a certain pore size, it is used in various separation and purification fields. However, the high reactivity of acyl chloride has the advantage of a fast reaction rate, which means that it is difficult to control chemically to have a certain performance in other aspects. Therefore, in this study, we observed how the chemical change of 1,3,5-benzenetricarbonyl trichloride affected the membrane performance.

• Journal of Sensor Science and Technology
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• v.8 no.6
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• pp.427-433
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• 1999

In this paper, we have presented an one-axis fluxgate magnetic sensor with ferrite core, excitation, and pick-up coil. This magnetometer is consist of a sensing element, driving circuits for excitation coil and signal processing for detecting second harmonic frequency component which is proportional to the DC magnetic to be measured. The sensor core is excited by a square waveform of voltage through 82 turns of the excitation coil. The second harmonic output of pick-up coil(150 turns) is measured by a FFT spectrum analyzer. This result is compared to output of PSD(phase sensitive detector) unit for detecting a second harmonic component. The measured sensitivity is about 50 V/T at driving frequency of 2 kHz. The nonlinearity of fluxgate magnetic sensor is calculated about 2.0%.

• Journal of Biosystems Engineering
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• v.39 no.1
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• pp.21-33
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• 2014

Background: Significant technological development and changes happened in the tractor industries. Contrariwise, the test procedures of the major standard development organizations (SDO's) remained unchanged or with a little modification over the years, demanding new tractor test standards or improvement of existing ones for tractor performance, safety, and comfort. Purpose: This study focuses on reviewing the research trends regarding performance, safety and comfort evaluation of agricultural tractors. Based on this review, few recommendations were proposed to revise or improve the current test standards. Review: Tractor power take-off power test using the DC electric dynamometer reduced human error in the testing process and increased the accuracy of the test results. GPS signals were used to determine acceleration and converted into torque. High capacity double extended octagonal ring dynamometer has been designed to measure drawbar forces. Numerical optimization methodology has been used to design three-point hitch. Numerous technologies, driving strategies, and transmission characteristics are being considered for reducing emissions of gaseous and particulate pollutants. Engine emission control technology standards need to be revised to meet the exhaust regulations for agricultural tractors. Finite Element Analysis (FEA) program has been used to design Roll-Over Protective Structures (ROPS). Program and methodology has been presented for testing tractor brake systems. Whole-body vibration emission levels have been found to be very dependent upon the nature of field operation performed, and the test track techniques required development/adaptation to improve their suitability during standardized assessment. Emphasizes should be given to improve visibility and thermal environment inside the cab for tractor operator. Tractors need to be evaluated under electromagnetic compatibility test conditions due to large growing of electronic devices. Research trends reviewed in this paper can be considered for possible revision or improvement of tractor performance, safety, and comfort test standards.

• Korean Journal of Bronchoesophagology
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• v.1 no.1
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• pp.136-141
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• 1995

Tracheostomal stenosis after total laryngectomy is a distressing complication which con-tributes significantly to both psychosocial and physical morbidity according to nature and severity in laryngectomee. Sternal stenosis will compromise not only optimal air exchange, crust formation but also the ability to clear tracheobronchial secretion, so pneumonia and atelectasis will develop. Having a number of procedure recommended for correction of such stenosis with limited results. We developed new technique which is based on tracheal advancement flap had been ap-plied to 12 patients, successfully. We think that total or partial tracheal advancement flap technique Is useful for widening the stoma and advantages of this method are following. 1. Simple technique. possible under local anesthesia 2. Healthy tracheal ring facilities width control 3. Less chance of refractory scar stenosis 4. Tracheoesophageal shunt can be constructed after the partial advancement flap.

• Journal of Environmental Science International
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• v.19 no.6
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• pp.747-753
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• 2010

It follows in quality and sewage exclusion method of the investigation objective sector and the Combined Sewer Overflows which is suitable in regional characteristics and the confluence area against the rainfall initially a flow and the medulla and measurement - it analyzes the initial rainfall outflow possibility control plan which is suitable in the domestic actual condition and it proposes the monitor ring plan for the long-term flow and pollution load data accumulation. From the research which it sees the Infiltration water/Influent water and CSOs investigation it passes by the phase of hazard chain and Namwon right time 4 it does not hold reverse under selecting, Measurement it used the hazard automatic flow joint seal Sigma 910 machine and in case 15 minute interval of the I/I, it measured a flow at case 5, 15 minute standing of the CSOs. The water quality investigation for the water leakage investigation of the I/I and the sewage from the point which is identical with flow measurement during on-the-spot inspection duration against 6 items which include the BOD sampling and an analysis, when the rainfall analysis for CSOs fundamental investigation analyzed against 18 items which include the BOD sampling. Consequently, for the optimum interpretation invasion water / inflow water of the this investigation area day average the lowest flow - water quality assessment veterinarian optimum interpretation hazard average per day - lowest flow - it averages a medulla evaluation law department one lowest flow evaluation technique and it selects, it presentation collectively from here it gets, position result with base flow analysis of invasion water / inflow water.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.18 no.12
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• pp.3029-3034
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• 2014

The paper describes the scleral diagnostic system implementation for human eyes by using the scleral color code and vessels sign pattern code generations. The system is based on the high performance DSP image signal processor, programmable gain control for preprocessing and RISC SD frames storage. RGB image signals are optimized by PGC, the edge image is detected form the gray image converted. The processing algorithms are executed by scleral color code generation and scleral vessels sign pattern code creation for discriminating and matching. The scleral symptomatic color code is generated by YCbCr values at memory map tolerated and the vessel sign pattern code is created by digitizing the 24 clock and 13 ring zones, overlay matching and tolerances. The experimental results for performance are that the system runs 40ms, and the color and pattern for diagnostic errors are around 20% and 24% on average. The system and technique enable a scleral diagnosis with subdividing the patterns and patient database.