• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.203-206
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• 2005

본 연구에서는 생물반응기에서 효율적으로 돼지 전염성 백신을 생산하기 위해 swine testicle cell을 host cell로 하는 백신 생산 시스템을 확립하였고, 이를 위한 최적 MOI, 최적 감염시기, 최적 수확시기 등 백신 생산 조건을 확립하였다. 또한 보다 안전한 백신 생산을 위해 통계적 방법에 의해 무 혈청 배지를 개발하였고 백신 생산 면에서의 가장 우수한 무 혈청 배지를 선정하였다.

• 한국식품영양과학회지
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• 제33권3호
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• pp.500-504
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• 2004

산초는 우리나라를 비롯한 일본, 중국 등지 에 널리 분포하고 있으며 전통적으로 우리 정서에 친숙한 식미와 향기를 가지는 아시아권의 공통적인 향신료이다. 더불어 우리나라에서는 생선회와 어패류의 소비가 증가함에 따라 Vibrio parahaemolyticus에 의한 식중독발생 억제방안의 확립이 필요한 실정이다. 따라서 본 연구는 독성이 없는 새로운 천연 식품 보존제의 개발을 위하여 식품 및 향신료로서 적용 범위가 광범위한 산초를 대상으로 Vibrio parahaemolyticus에 대한 천연항균제의 적용 가능성을 검토하였다. 산초나무 추출물의 항균작용을 알아보기 위해서 Vibrio parahaemolyticus에 대한 항균력 분석에서 종자를 제외한 성분으로 제조한 산초의 주정추출물이 효과가 가장 우수함을 확인하였으며, 이 추출물의 온도 및 pH에 대한 안정성을 검토한 결과 다양한 범위의 온도, pH에 대해서 매우 안정하다는 것을 알 수 있었다. 또한 GC-MS에 의한 산초 추출물의 분리, 동정 결과에서는 estragole이 항균활성을 나타내는 주성분임을 확인하였다. 이 결과를 바탕으로 우리나라에서 많이 사용되는 산초를 Vibrio parahaemolyticus에 의한 식중독을 줄일 수 있는 항균제로 적용할 수 있음을 확인할 수 있었다. 따라서 식품첨가제 및 포장재, 식품포장 외부 충진 물질, 살균소독제 등의 다양한 형태로의 응용이 가능하리라 생각된다.

• 한국식품위생안전성학회지
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• 제12권2호
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• pp.132-140
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• 1997

Additives in plastics are capable of migrating from the packaging materials into the foodstuffs, thereby presenting a source of contamination and a potential health risk to the consumer. The migration from packaging materials into foodstuffs is first of all regulated by examining the amounts of global and specific migrated components. Besides, there is worldwide still a need for practical methods for measuring and monitoring migration from polymers, especially for the testing of migration into fatty foodstuffs. Therefore, these studies were undertaken to investigate the safety status of domestic plastic packaging materials with respect to migration. Another objective of this study was to examine the applicability of ethanol as an alternative fatty food simulant substituting for olive oil and n-heptane. The evaporation residues for various dometic plastic samples determined as described in Korean food laws were in the level from 4.3 to 14.5 mg/$\ell$, which were much lower than the limit value of 150 mg/$\ell$. The global migration values into 95 % ethanol showed to be comparable to those into n-heptane, while the olive oil migration values were comparably higher than those into ethanol or n-heptane and moreover they were not reproducible. The kinetic migration begavior of additives in polyolefin samples into 95% ethanol showed a Fickian diffusion process. The results of these studies on global migration and kinetic testings demonstrate that the ethanol could be successfully substitute for the olive oil and n-heptane as an alternative fatty food simulant, at least in contact with polyoefins.

• 한국축산식품학회지
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• 제40권4호
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• pp.578-587
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• 2020

Clostridioides (Clostridium) difficile is a Gram (+), anaerobic, spore forming, rod shaped bacterium that can produce toxin. The objective of this study is to reveal the presence of C. difficile in meat products, to analyze the ribotype diversity by PCR and to evaluate the antibiotic susceptibility of isolated strains. The organism was isolated in 22 out of 319 (6.9%) examined meat product samples and 9 out of 22 (40.9%) isolates were identified as RT027 and all isolates had the ability of toxin production. In terms of antibiotic susceptibility, all isolates were susceptive to amoxicillin-clavulanic acid, tetracycline and vancomycin and 21 (95.4%) isolates to metronidazole. On the other hand, imipenem and cefotaxim resistance was observed in all. In conclusion, the results of this comprehensive study conducted in Turkey deduced the presence of C. difficile in different meat products. Therefore, these products can be evaluated as a potential contamination source of C. difficile from animals to humans especially for elders, youngsters, long terms wide spectrum antibiotic used and immuno-suppressed individuals.

• 한국산업보건학회지
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• 제5권2호
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• pp.137-146
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• 1995

Twenty(20) large commercial buildings located in Seoul with friable sprayed-on surface insulation material on ceilings were investigated for asbestos content in bulk material by polarized light microscopy and for airborne fiber concentrations in buildings by phase contrast microscopy. In addition, such building-related variables as building age, numbers of traffic, airflow, surface conditions of the ceiling, temperature, and humidity were studied for any correlation with airborne fiber concentrations. The results were as follows: 1. Chrysotile asbestos was found in two bulk samples with 3-5% content and with <1%in one sample out of total 20 bulk samples collected. Glass fiber and mineral wool were the two major constituents of the bulk samples. 2. The ceiling surfaces were very friable in 16 buildings and were relatively hard in 4 buildings. The friability of the surface material was dependent upon the type and the amount of binder that had been mixed with the sprayed-on surface material. 3. Airborne fiber concentrations were log-normally distributed and the geometric mean(geometric standard deviation) fiber concentrations in the underground parking lots, inside buildings, and outdoor ambient air were 0.0063(1.97)f/cc, 0.0068(2.29)f/cc, and 0.0033(2.36)f/cc, respectively. 4. No significant relationship of airborne fiber concentrations and all building-related variables studied except humidity was found. The results of this study suggest that the sprayed-on surface insulation material found in some commercial buildings may possibly be contaminated with asbestos. Since most of the ceiling surfaces surveyed were very friable and poorly maintained and the airborne fiber concentrations were relatively high, there is a possibility of asbestos fiber contamination in these buildings, particularly at those buildings with asbestos-contaminated surface material. Since poorly maintained surface conditions were thought to be a source of high airborne fiber concentrations, there is a urgent need of a systematic operation and maintenance program. Further study of non-occupational asbestos exposure in general population utilizing advanced analytical technique such as transmission electron microscopy is highly recommended.

• 한국지하수토양환경학회지:지하수토양환경
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• 제25권3호
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• pp.43-51
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• 2020

About 90% of groundwater wells in Jeju Island are reported to be under the threat of contamination by infiltration of the surface pollutants. Most of those wells have improperly grouted annulus which is an empty space between the well and the inner casing. As a remedy to this problem, some of the wells were re-grouted by filling the annulus with cement without lifting an inner casing. In order to evaluate whether this method is appropriate for the geological structure of Jeju Island, two wells (W1 and W2) were selected and this method was applied. The water holding capacity did not decrease while the nitrate levels decreased from 16.8 and 20.2 to 6.8 and 13.8 mg/L in W1 and W2, respectively. The higher nitrate level in W2 is deemed to be influenced by the livestock farms located in the upper area of the well. In addition, transmissivity of the vedose zone was higher in W2 than W1, potentially facilitating the transport of nitrate to the groundwater. The overall result of this study suggests re-grouting of wells for the purpose of protecting water quality of goundwater should take into account geological structure of vadose zone as well as appropriate source control of the contaminants.

• 한국토양비료학회지
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• 제47권6호
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• pp.391-397
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• 2014

Acid mine drainage occurrence is a serious environmental problem by mining industry; it usually contain high levels of metal ions, such as iron, copper, zinc, aluminum, and manganese, as well as metalloids of which arsenic is generally of greatest concern. It causes mine impacted soil pollution with mining and smelting activities, fossil fuel combustion, and waste disposal. In the present study, three bacterial strains capable of producing urease were isolated by selective enrichment of heavy metal contaminated soils from a minei-mpacted area. All isolated bacterial strains were identified Sporosarcina pasteurii with more than 98% of similarity, therefore they were named Sporosarcina sp. KM-01, KM-07, and KM-12. The heavy metals detected from the collected mine soils containing bacterial isolates as Mn ($170.50mg\;kg^{-1}$), As ($114.05mg\;kg^{-1}$), Zn ($92.07mg\;kg^{-1}$), Cu ($62.44mg\;kg^{-1}$), and Pb ($40.29mg\;kg^{-1}$). The KM-01, KM-07, and KM-12 strains were shown to be able to precipitate calcium carbonate using urea as a energy source that was amended with calcium chloride. SEM-EDS analyses showed that calcium carbonate was successfully produced and increased with time. To confirm the calcium carbonate precipitation ability, urease activity and precipitate weight were also measured and compared. These results demonstrate that all isolated bacterial strains could potentially be used in the bioremediation of acidic soil contaminated by heavy metals by mining activity.

• 환경영향평가
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• 제19권3호
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• pp.215-222
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• 2010

This research aims to gain the result of assessment of inflow categorized by runoff path using DIROM (Daily Irrigation Reservoir Operation Model) for Baengnyeong-myeon reservior which was built for residents of Baengnyeong island to solve the shortage of drinking water and stable supply of domestic water. The simulation results of DIROM and actual hydrograph of the reservoir show very low correlation with geological characteristics. The simulation results by DIROM after adjusting with modified Tank III model which considers all outflow from Tank II model as interflow among 3 level tanks show good correlation of its regional runoff and inflow characteristics with $R^2$=0.9058. In the study area, diffluence of 37% of rain fall of the study year has been simulated, which shows low result compared to the average river runoff of national water resource. In addition, 34.5% of total inflow to the study reservoir is mainly interflow and baseflow among expected several channels.

• Parasites, Hosts and Diseases
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• 제56권2호
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• pp.205-210
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• 2018

Waterborne parasitic protozoa, particularly Giardia lamblia and Cryptosporidium spp., are common causes of diarrhea and gastroenteritis worldwide. The most frequently identified source of infestation is water, and exposure involves either drinking water or recreation in swimming pools or natural bodies of water. In practice, studies on Cryptosporidium oocysts and Giardia cysts in surface water are challenging owing to the low concentrations of these microorganisms because of dilution. In this study, a 3-year monitoring of Cryptosporidium parvum, Giardia lamblia, and Naegleria fowleri was conducted from August 2014 to June 2016 at 5 surface water sites including 2 lakes, 1 river, and 2 water intake plants. A total of 50 water samples of 40 L were examined. Cryptosporidium oocysts were detected in 22% of samples and Giardia cysts in 32%. Water at the 5 sampling sites was all contaminated with Cryptosporidium oocysts (0-36/L), Giardia cysts (0-39/L), or both. The geometric mean concentrations of Cryptosporidium and Giardia were 1.14 oocysts/L and 4.62 cysts/L, respectively. Thus, effective monitoring plans must take into account the spatial and temporal parameters of contamination because they affect the prevalence and distribution of these protozoan cysts in local water resources.

• Environmental Engineering Research
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• 제12권4호
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• pp.176-193
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• 2007

The ability to measure groundwater contaminant flux is increasingly being recognized as crucial in order to prioritize contaminated site cleanups, estimate the efficiency of remediation technologies, measure rates of natural attenuation, and apply proper source terms to model groundwater contaminant transport. Recently, a number of methods have been developed and subsequently applied to measure contaminant mass flux in groundwater in the field. Flux measurement methods can be categorized as either point methods or integral methods. As the name suggests, point methods measure flux at a specific point or points in the subsurface. To increase confidence in the accuracy of the measurement, it is necessary to increase the number of points (and therefore, the cost) of the sampling network. Integral methods avoid this disadvantage by using pumping wells to interrogate large volumes of the subsurface. Unfortunately, integral methods are expensive because they require that large volumes of contaminated water be extracted and managed. Recent work has investigated the development of an integral method that does not require extraction of contaminated water from the subsurface. We begin with a review of the significance and importance of measuring groundwater contaminant mass flux. We then review groundwater contaminant flux measurement methods that are either currently in use or under development. Finally, we conclude with a qualitative comparison of the various flux measurement methods.