• Molecules and Cells
• /
• v.28 no.6
• /
• pp.529-536
• /
• 2009

Genome sequencing of the pig is being accelerated because of its importance as an evolutionary and biomedical model animal as well as a major livestock animal. However, information on expressed porcine genes is insufficient to allow annotation and use of the genomic information. A series of expressed sequence tags of 5' ends of five full-length enriched cDNA libraries (SUSFLECKs) were functionally characterized. SUSFLECKs were constructed from porcine abdominal fat, induced fat cells, loin muscle, liver, and pituitary gland, and were composed of non-normalized and normalized libraries. A total of 55,658 ESTs that were sequenced once from the 5′ ends of clones were produced and assembled into 17,684 unique sequences with 7,736 contigs and 9,948 singletons. In Gene Ontology analysis, two significant biological process leaf nodes were found: gluconeogenesis and translation elongation. In functional domain analysis based on the Pfam database, the beta transducin repeat domain of WD40 protein was the most frequently occurring domain. Twelve genes, including SLC25A6, EEF1G, EEF1A1, COX1, ACTA1, SLA, and ANXA2, were significantly more abundant in fat tissues than in loin muscle, liver, and pituitary gland in the SUSFLECKs. These characteristics of SUSFLECKs determined by EST analysis can provide important insight to discover the functional pathways in gene networks and to expand our understanding of energy metabolism in the pig.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.2 no.2
• /
• pp.50-61
• /
• 2000

The ground around the portal of a tunnel is the most typical part showing the 3-dimensional mechanical behavior in the tunnel. The portal slope is constructed at the weathered soft rock-mass, and remains as a potential sliding mass. The slope failure around the tunnel portal may happen drastically and induce the great disaster; hence, for the permanent stability several special techniques are required. To solve this problem, the ground around the tunnel portal may be excavated in the arch shape to develop the arching effect in horizontal direction. With the arch-type portal slope, one can reduce considerably the excavation mass and the damage of environments. This approach has not been attempted yet due to the lack of understanding and the well-defined analyzing method, so the retaining wall type portal is more universal. The 3-dimensional finite element analyses were carried out to prove that the arch type is more advantageous in safety and cost than the right angle type. The influence of the tunnel construction sequence and the strength of the rock-mass on the slope stability was investigated by focusing on the maximum shear strain in the slope, and the yield zone at the tunnel face.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.3 no.1
• /
• pp.1-12
• /
• 1983

The wave and hydrologic climate at a beach location are everchanging and coastal engineers have always been interested in the deformation of the natural beach caused by wave action over short or long interval of time. The drift of sand on a beach particulary manifests itself when blocked construction of seawalls, jetties, breakerwaters and groins etc.${\cdots}$ For this reason, the understanding and evaluation of littoral drift has been especially important to the coastal engineers. The resulting of the sand drift movement, such as the type of beach profile, width of beach, storage volume of the littoral sand over the profile are rapid everchange. We have studied the geological changes due to the littoral drift on a beach with field investigation and model tests in laboratory. But, it is impossible to make quantative correct analysis because of the factors are everchange and complicate. And then, most of study are incline to qualitative analysis. In this paper, authors studied mainly on the transition of beach profile and sediment storage on the profile using statistical field data as qualitative analytical method. The used theoretical beach transition model by Sonu and Beek have developed to obtain the change of HAEUNDAE beach backed with seawall. Results of this study indicate that the transition model are useful in the analysis of beach profile changement and the littoral drift movement on the beach. Qualitative analysises for HAEUNDAE beach are as follows. 1) Transition sequence of profile has 4 major transition for one cycle. 2) Storage sediment model of beach profile by Sonu and Beek well coincided with HAEUNDAE beach. 3) Seasonal cycle has ill-balanced process for the 5-yr. investigation.

• Horticultural Science & Technology
• /
• v.32 no.6
• /
• pp.853-863
• /
• 2014

This study was carried out to construct a DNA profile database of 100 strawberry cultivars using microsatellite markers. Two hundred seventy four microsatellite primer pairs were screened with a set of 21 strawberry cultivars with different morphological traits. Twenty five primer pairs were selected because they produced reliable and reproducible fingerprints. These primer pairs were used to develop DNA profiles of 100 strawberry cultivars. Three to thirteen alleles were detected by each marker with an average of 7.50. The average polymorphism information content varied from 0.331 to 841 (average 0.706). Cluster analysis showed that the 100 cultivars were divided into 7 major groups reflecting geographic origin and pedigree information. Moreover, most of the cultivars could be discriminated by marker genotypes. These markers will be useful as a tool for the protection of plant breeders' intellectual property rights in addition to providing the means to intervene seed disputes relating to variety authentication.

• Journal of Life Science
• /
• v.19 no.6
• /
• pp.809-817
• /
• 2009

The phenotypes associated with a gene function are often the best clue to its role in the plant. Transgenic plants ectopically expressing or suppressing a gene can provide useful information related to the gene function. In this study, we constructed three vectors - pFGL571, pFGL846 and pFGL847 - for the Agrobacterium-mediated ectopic expression of plant genes using pPZP211 and modified CaMV 35S, UBQ3 or UBQ10 promoters. The three vectors have several merits such as small size, high copy in bacteria, enough restriction enzyme sites in multi cloning sites and nucleotide sequence information. Analysis of transgenic plants containing GUS or sGFP reporter genes under the control of modified CaMV 35S, UBQ3 or UBQI0 promoter revealed that all of the three promoters showed high activities during most developmental stages after germination and in floral organs. Furthermore, we generated a RNAi module vector, pFGL727, to suppress plant gene expressions and confirmed that pFGL727 is useful for the suppression of a gene expression using rice transgenic plants. Taken together, our new vectors would be very useful for the ectopic expression or the suppression of plant genes.

• Korean Journal of Ecology and Environment
• /
• v.38 no.2 s.112
• /
• pp.137-145
• /
• 2005

Traditional screening techniques have missed up to 99% of microbial resources existing in the nature. Strategies of direct cloning of environmental DNAs comprising tine genetic blueprints of entire microbial metagenomes provide vastly more genetic information than is contained in the culturable. Therefore, one way to screening the useful gene in a variety of environments is the construction of metagenomic DNA library. In this study, the water samples were collected from Daecheong Reservoir in the mid Korea, and analyzed by T-RFLP to examine the diversity of the microbial communities. The crude DNAs were extracted by SDS-based freezing-thawing method and then further purified using an $UltraClean^{TM}kit$ (MoBio, USA). The metagenomic libraries were constructed with the DNAs partially digested with EcoRI, BamHI, and SacII in Escherichia coli DH10B using the pBACe3.6 vector. About 14.0 Mb of metagenomic libraries were obtained with average inserts 13 ${\sim}$ 15 kb in size. The genes responsible for degradation of 1, 2-dichloroethane (1, 2-DCE) via hydrolytic dehalogenation were identified from the metagenomic libraries by colony hybridization. The 1, 2-dichloroethane dehalogenase gene (dhlA) was cloned and its nucleotide sequence was analyzed. The activity of the 1, 2-DCE dehalogenase was highly expressed to the substrate. These results indicated that the dhlA gene identified from the metagenomes derived from Deacheong Reservoir might be useful to develop a potent strain for degradation of 1, 2-DCE.

• Journal of The Korean Society of Agricultural Engineers
• /
• v.59 no.3
• /
• pp.83-96
• /
• 2017

This study presented a surface water quality modeling framework considering the spatial resolution of pollutant load estimation to better represent stream water quality characteristics in the Saemangeum watershed which has been focused on keeping its water resources sustainable after the Saemangeum embankment construction. The watershed delineated into 804 sub-watersheds in total based on the administrative districts, which were units for pollutant load estimation and counted as 739 in the watershed, Digital Elevation Model (DEM), and agricultural structures such as drainage canal. The established model consists of 7 Mangyung (MG) sub-models, 7 Dongjin (DJ) sub-models, and 3 Reclaimed sub-models, and the sub-models were simulated in a sequence of upstream to downstream based on its connectivity. The hydrologic calibration and validation of the model were conducted from 14 flow stations for the period of 2009 and 2013 using an automatic calibration scheme. The model performance to the hydrologic stations for calibration and validation showed that the Nash-Sutcliffe coefficient (NSE) ranged from 0.66 to 0.97, PBIAS were -31.0~16.5 %, and $R^2$ were from 0.75 to 0.98, respectively in a monthly time step and therefore, the model showed its hydrological applicability to the watershed. The water quality calibration and validation were conducted based on the 29 stations with the water quality constituents of DO, BOD, TN, and TP during the same period with the flow. The water quality model were manually calibrated, and generally showed an applicability by resulting reasonable variability and seasonality, although some exceptional simulation results were identified in some upstream stations under low-flow conditions. The spatial subdivision in the model framework were compared with previous studies to assess the consideration of administrative boundaries for watershed delineation, and this study outperformed in flow, but showed a similar level of model performance in water quality. The framework presented here can be applicable in a regional scale watershed as well as in a need of fine-resolution simulation.

• Journal of the Korean Society for Marine Environment & Energy
• /
• v.12 no.3
• /
• pp.188-196
• /
• 2009

This study conducted numerical simulations to analyze the wave characteristics(distribution) depending on the directional changes of waves in the Nakdong river estuary by using SWAN(Simulating WAves Nearshore) model. The results from the tests are summarized as below. The wave height rates are generally highly distributed with the incident waves from the S, SSE, SSW, SE, SW in sequence. When the waves from the S, SSW, SSE directions are predominant, the bigger waves were observed in front of sandbars. According to the results of the wave steepness against the wave direction, at the east coast of Gadeok island(northwest of Nakdong estuary), where has mild seabed slopes, the wave height rates distribute in the range of 0.4~0.6; the wave height rates over the west coastal region of Dadeapo(southeast of Nakdong estuary) are 0.5~0.6. The wave height rate tends to be rapidly decreased over the east region of Nakdong river estuary rather than its west region.

• Nuclear Engineering and Technology
• /
• v.40 no.6
• /
• pp.451-458
• /
• 2008

After more than 10 years construction, KSTAR (Korea Superconducting Tokamak Advanced Research) had finally completed its assembly in June 2007, and then achieved the goal of first-plasma in July 2008 through the four month's commissioning. KSTAR was constructed with fully superconducting magnets with material of $Nb_3Sn$ and NbTi, and their operation temperatures are maintained below 4.5K by the help of Helium Refrigerator System. During the first-plasma operation, plasmas of maximum current of 133kA and maximum pulse width of 865ms were obtained. The KSTAR Integrated Control System (KICS) has successfully fulfilled its missions of surveillance, device operation, machine protection interlock, and data acquisition and management. These and more were all KSTAR commissioning requirements. For reliable and safe operation of KSTAR, 17 local control systems were developed. Those systems must be integrated into the logically single control system, and operate regardless of their platforms and location installed. In order to meet these requirements, KICS was developed as a network-based distributed system and adopted a new framework, named as EPICS (Experimental Physics and Industrial Control System). Also, KICS has some features in KSTAR operation. It performs not only 24 hour continuous plant operation, but the shot-based real-time feedback control by exchanging the initiatives of operation between a central controller and a plasma control system in accordance with the operation sequence. For the diagnosis and analysis of plasma, 11 types of diagnostic system were implemented in KSTAR, and the acquired data from them were archived using MDSpius (Model Driven System), which is widely used in data management of fusion control systems. This paper will cover the design and implementation of the KSTAR integrated control system and the data management and visualization systems. Commissioning results will be introduced in brief.

• Journal of the Institute of Electronics Engineers of Korea SC
• /
• v.39 no.6
• /
• pp.42-48
• /
• 2002

The design, construction and performance test of a convenient multi-purpose irradiator is described. A multi-purpose irradiator using Cesium-137 has been developed for studies of low dose radiation effects in biology and for calibration of Thermo Luminescent dosimeter(TLD). During the operation, three rods of radioactive material which are 10cm in length revolve 180 degrees and irradiate biological samples, or TLD, and return to their shielded position, after the programmed time. A programmable Logic Controller(PLC) controls the sequence of operation, interlock, motor rotation and safety system. The rotation speed of biological samples can vary up to 20 RPM. A real time monitoring system was also incorporated to check and control the operation status of the irradiator. The capacity of the irradiation chamber was 4.5 liters. The isodose distribution at arbitrary vertical planes was measured by using film dosimetry. The dose-rate was 0.13 cGy/min in air and 0.11 cGy/min in water equivalent material in the case of Cesium-137. Range of activity was 2 Ci. The homogeneity of dose distribution in the chamber was ${\pm}$7%. The actual radiation level on the surface was within permissible levels. The irradiator had a maximum 0.35 mR/min radiation leakage on its surface.