• Journal of fish pathology
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• v.24 no.3
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• pp.297-305
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• 2011

A trap identification system for isolating functional sequences to allow the constitutive expression of foreign protein from Edwardsiella tarda was developed. Using the green fluorescent protein (GFP) reporter-based trap system, various functional sequences to drive heterologous expression of the GFP were selectable in Escherichia coli host. However from the bioinformatic sequence analysis, all the segments predicted as regulatory regions were not native promoters actually existing upstream of endogenous E. tarda genes. Instead, a number of non-authentic sequences, possibly resulted from the random shuffling and/or intermolecular ligation were also proven to be able to display a potent GFP expression in the recombinant E. coli. Further analysis with selected clones showed that both authentic and non-authentic sequences could function in as a constitutive promoter, leading quite a consistent and stable GFP expression after repetitive subcultures. Microscopic examination also confirmed the uniform pattern of GFP expression in every host bacterium. Semi-quantitative assay of GFP showed that there was no clear relationship between expression levels and organizational features of the promoters trapped. Functional promoter-like elements achieved in the present study could be a good starting material for multivalent genetic engineering of E. tarda in order to produce recombinant vaccines in a cost-effective fashion.

• International Journal of Automotive Technology
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• v.1 no.1
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• pp.35-41
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• 2000

The crashworthiness of vehicles with finite element methods depends on the geometry modeling and the material properties. The vehicle body structures are generally composed of various members such as frames, stamped panels and deep-drawn parts from sheet metals. In order to ensure the impact characteristics of auto-body structures, the dynamic behavior of sheet metals must be examined to provide the appropriate constitutive relation. In this paper, high strain-rate tensile tests have been carried out with a tension type split Hopkinson bar apparatus specially designed for sheet metals. Experimental results from both static and dynamic tests with the tension split Hopkinson bar apparatus are interpolated to construct the Johnson-Cook and a modified Johnson-Cook equation as the constitutive relation, that should be applied to simulation of the dynamic behavior of auto-body structures. Simulation of auto-body structures has been carried out with an elasto-plastic finite element method with explicit time integration. The stress integration scheme with the plastic predictor-elastic corrector method is adopted in order to accurately keep track of the stress-strain relation for the rate-dependent model accurately. The crashworthiness of the structure with quasi-static constitutive relation is compared to the one with the rate-dependent constitutive model. Numerical simulation has been carried out for frontal frames and a hood of an automobile. Deformed shapes and the Impact energy absorption of the structure are investigated with the variation of the strain rate.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2004.05a
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• pp.233-236
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• 2004

The major objective of this paper is to clarify the effect of constitutive laws on bulk forming design based on the ideal flow theory. The latter theory is in general applicable for perfectly/plastic materials. However, its kinematics equations constitute a closed-form system, which are valid for any incompressible materials, therefore enabling us to extend design solutions based on the perfectly/plastic constitutive law to more realistic laws with rate sensitive hardening behavior. In the present paper, several constitutive laws commonly accepted for the modeling of cold and hot metal forming processes are considered and the effect of these laws on one particular plane-strain design is demonstrated. The closed form solution obtained describes a non-trivial nonsteady ideal process. The design solutions based on the ideal flow theory are not unique. To achieve the uniqueness, the criterion that the plastic work required to deform the initial shape of a given class of shapes into a prescribed final shape attains its minimum is adopted. Comparison with a non-ideal process is also made.

• Structural Engineering and Mechanics
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• v.18 no.5
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• pp.591-607
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• 2004

The substance of the use of the derived non-linear creep constitutive equations under variable stress levels (see first part of the paper, Kmet 2004) is explained and the strategy of their application is outlined using the results of one-step creep tests of the steel spiral strand rope as an example. In order to investigate the creep strain increments of cables an experimental set-up was originally designed and a series of tests were carried out. Attention is turned to the individual main steps in the production and application procedure, i.e., to the one-step creep tests, definition of loading history, determination of the kernel functions, selection and definition of constitutive equation and to the comparison of the resulting values considering the product and the additive forms of the approximation of the kernel functions. To this purpose, the parametrical study is performed and the results are presented. The constitutive equations of non-linear creep of cable under variable stress history offer a strong tool for the real simulation of stochastic variable load history and prediction of realistic time-dependent response (current deflection and stress configuration) of structures with cable elements. By means of suitable stress combination and its gradual repeating various loads and times effects can be modelled.

• Fibers and Polymers
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• v.7 no.1
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• pp.42-50
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• 2006

In this paper, elasto-plastic constitutive equations for highly anisotropic and asymmetric materials are developed and their numerical implementation is presented. Some engineering materials such as fiber reinforced composites show different material behavior in the different material directions (anisotropy) as well as in tension and compression (asymmetry). Although these materials have mostly been analyzed using the anisotropic elastic constitutive equations, the necessity of consideration of plastic properties has been frequently reported in the previous works. In order to include both the anisotropic and asymmetric properties of composite materials, the Drucker-Prager yield criterion is modified by adding anisotropic parameters and initial components of translation. The implementation procedure for the developed theory and algorithms is presented based on the implicit finite element scheme. The measured data from the previous work are used to validate the present constitutive equations.

• Geomechanics and Engineering
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• v.5 no.3
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• pp.241-261
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• 2013

The bearing mechanism of pile during installation and loading process which controls the deformation and distribution of strain and stress in the soil surrounding pile tip is complex and full of much uncertainty. It is pointed out that particle crushing occurs in significant stress concentrated region such as the area surrounding pile tip. The solution to this problem requires the understanding and modeling of the mechanical behavior of granular soil under high pressures. This study aims to investigate the sand behavior around pile tip considering the characteristics of sand crushing. The numerical analysis of model pile loading test under different surcharge pressure with constitutive model for sand crushing is presented. This constitutive model is capable of predicting the dilatancy of soil from negative to positive under low confining pressure and only negative dilatancy under high confining pressure. The predicted relationships between the normalized bearing stress and normalized displacement are agreeable with the experimental results during the entire loading process. It is estimated from numerical results that the vertical stress beneath pile tip is up to 20 MPa which is large enough to cause sand to be crushed. The predicted distribution area of volumetric strain represents that the distributed area shaped wedge for volumetric contraction is beneath pile tip and distributed area for volumetric expansion is near the pile shaft. It is demonstrated that the finite element formulation incorporating a constitutive model for sand with crushing is capable of producing reasonable results for the pile loading problem.

• Journal of the Society of Naval Architects of Korea
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• v.45 no.5
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• pp.538-546
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• 2008

In this paper, the constitutive equation is developed to analyze the characteristics of strain-induced plasticity in the range of low temperature of 316 stainless steel. The practical usefulness of the developed equations is evaluated by the comparison between experimental and numerical results. For 316 stainless steel, constitutive equations, which represent the characteristics of nonlinear material behavior under the cryogenic temperature environment, are developed using the Bodner's plasticity model. In order to predict the material behaviour such as damage accumulation, Bodner-Chan's damage model is implemented to the developed constitutive equations. Based on the developed constitutive equations, 3-D finite element analysis program is developed, and verified using experimental results.

• KCI Concrete Journal
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• v.13 no.1
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• pp.19-25
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• 2001

Based on the orthotropic hypoelasticity formulation, a constitutive material model of concrete taking account of triaxial stress state is presented. In this model, the ultimate strength surface of concrete in triaxial stress space is described by the Hsieh's four-parameter surface. On the other hand, the different ultimate strength surface of concrete in strain space is proposed in order to account for increasing ductility in high confinement pressure. Compressive ascending and descending behavior of concrete is considered. Concrete cracking behavior is considered as a smeared crack model, and after cracking, the tensile strain-softening behavior and the shear mechanism of cracked concrete are considered. The proposed constitutive model of concrete is compared with some results obtained from tests under the states of uniaxial, biaxial, and triaxial stresses. In triaxial compressive tests, the peak compressive stress from the predicted results agrees well with the experimental results, and ductility response under high confining pressure matches well the experimental result. The reinforcing bars embedded in concrete are considered as an isoparametric line element which could be easily incorporated into the isoparametric solid element of concrete, and the average stress - average strain relationship of the bar embedded in concrete is considered. From numerical examples for a reinforced concrete simple beam and a structural beam type member, the stress state of concrete in the vicinity of talc critical region is investigated.

• Journal of the Korean Geotechnical Society
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• v.20 no.7
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• pp.79-89
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• 2004

An elastic-plastic-viscous constitutive model was proposed based on a simple formulation scheme. The anisotropic modified Cam-Clay model was extended for the general stress space for the plastic simulation. The generalized viscous theory was simplified and used for the viscous constitutive part. A damage law was incoporated into the proposed constitutive model. The mathematical formulation and development of the model were performed from the point of view that fewer parameters be better employed. The creep behaviors with or without creep rupture were predicted using the developed model for cohesive soils. The model predictions were favorably compared with the experimental results including the undrained creep rupture, which is an important observed phenomenon for cohesive soils. Despite the simplicity of the constitutive model, it performs well as long as the time to failure ratio of the creep rupture tests is within the same order of magnitude.

• Geotechnical Engineering
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• v.10 no.1
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• pp.7-18
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• 1994

A new model for describing the behavior of clay under monotonic and cyclic loading is proposed. This model uses the hyperbolic representation for the stress -strain relationship in overconsolidated state and it describes undrained effective stress path on the basis of the critical state theory. The developed constitutive model by using an energy dissipation equation can describe the behavior of clay in heavily overconsolidated state as u.ell as lightly overconsolidated state under monotonic loading. In order to extend the model for the behavior of clay under cyclic loading, a shift function of undrained stress spacing ratio is introduced in the constitutive model developed for monotonlc loading. A single additional parameter is required to represent the cyclic effect and it can be reasonably deter mined from the test results. The measured behavior in undrained cyclic triaxial tests has been easily and precisely predicted by the newly developed constitutive model.