• Journal of the Computational Structural Engineering Institute of Korea
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• v.12 no.3
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• pp.385-396
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• 1999

In the process of structural design for large-scale structures with several thousands of degrees of freedom, a plethora of structural calculations with large amount of data storage are required to obtain the forces and displacements of the members. However, current computational environment with single microprocessor such as a personal computer or a workstation is not capable of generating a high-level of efficiency in structural analysis and design process for large-scale structures. In this paper, a high-performance parallel computing system interconnected by a network of personal computers is proposed for an efficient structural analysis. Two distributed structural analysis algorithms are developed in the form of distributed or parallel preconditioned conjugate gradient (DPCG) method. To enhance the performance of the developed distributed structural analysis algorithms, the number of communications and the size of data to be communicated are minimized. These algorithms are applied to the structural analyses of three large space structures as well as a 144-story tube-in-tube framed structure.

• Journal of Korean Society of Steel Construction
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• v.11 no.2 s.39
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• pp.213-222
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• 1999

Elastic-plastic methods have been used for the better prediction of the actual behavior of continuous-composite plate girder bridges in the overload and maximum load analysis. The structural evaluation using ALFD(Alternate Load Factor Design) uses the elastic-plastic analysis. The plastic rotations that remain after the load is removed can be occurred by the yielding locations of the maximum moment section. This situation can occur due to the residual stresses even if the moment is below the theoretical yield moment. The local yielding causes positive automoments that assure elastic behavior under subsequent overloads. In this study, the automoments at the piers occurred due to the unit plastic rotations and other locations were calculated by the conjugate-beam method and three-moment equation, using the nine design span with progressively smaller pier sections. The automoments were determined by the developed computer programs in this study in which the moments and plastic rotations from the continuity and moment-inelastic rotation relationships must be equal. And also the ratings of 3-span continuous composite plate girder bridges with non-compact section were carried out according to the Korean Highway Bridge Specification.

• Journal of Microbiology and Biotechnology
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• v.17 no.10
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• pp.1629-1637
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• 2007

An immunochromatography (ICG) strip test using a nanocolloidal gold-antibody probe was developed and optimized for the rapid detection of aflatoxin B1 (AFB1). A monoclonal antibody specific to AFB1 was produced from the cloned hybridoma cell (AF78), coupled with nanocolloidal gold, and distributed on the conjugate pad of the ICG strip test. The visual detection limit of the ICG strip test was 0.5 ng/ml, and this method showed a cross-reaction to aflatoxin B2, G1, and G2. In total, 172 grain and feed samples were collected and analyzed by both the ICG strip test and HPLC. The results of the ICG strip test showed a good agreement with those obtained by HPLC. These results indicated that the ICG strip test has a potential use as a rapid and cost-effective screening tool for the determination of AFB1 in real samples and could be applied to the preliminary screening of mycotoxin in food and agricultural products, generating results within 15 min without complicated steps.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.10
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• pp.1498-1504
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• 2005

Competitive direct ELISA was developed to detect gentamicin residues. Mice immunized with gentamicin-keyhole limpet hemocyanin (KLH) conjugate developed good antiserum titers, which gradually increased with booster injections, indicating immunization was successfully processed. Monoclonal antibody against gentamicin was prepared using hybridoma cells cloned by limit dilution of fused cells. IgG was purified from ascites fluid of hybridoma cell-injected mice through ammonium sulfate precipitation and Sephadex G-25 gel filtration. After the gel filtration, fractions of high antibody titer were further purified through affinity chromatography on protein A/G column. Monoclonal antibody against gentamicin was confirmed as IgG1, which has kappa light chain. Cross-reactivities ($CR_{50}$) of gentamicin monoclonal antibody to other aminoglycosides (kanamycin, neomycin, and streptomycin) were less than 0.005%, indicating the monoclonal antibody was highly specific for gentamicin. Standard curve constructed through competitive direct ELISA showed measurement range (from 80 to 20% of B/$B_0$ ratio) of gentamicin was between 1 and 40 ng/ml, and 50% of B/$B_0$ ratio was about 4 ng/ml. The gentamicin concentration rapidly increased to 1,300 ng/ml after the intramuscular administration up to 2 h, then sharply decreased to less than 300 ng/ml after 4 h of withdrawal, during which the elimination half-life ($t_{1/2}$) of gentamicin in the rabbit plasma was estimated to be 1.8 h. Competitive direct ELISA method developed in this study using the prepared monoclonal antibody is highly sensitive for gentamicin, and could be useful for detecting gentamicin residues in plasma of live animals.

• Biomedical Science Letters
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• v.5 no.1
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• pp.11-15
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• 1999

Thyroxine (3,5,3',5'-L-tetraiodothyronine; T$_4$) is the most commonly measured thyroid hermono for the diagnosis of various thyroid disorders. Although radioimmunoassay (RIA) is still considered as the reference technique for the measurement of T$_4$, it is generally regarded that RIA has its primary disadventages in handling the wastes and controling the human and material resources. Therefore, establishment of enzyme-linked immunosorbent assay (ELISA) has of great significance. To verify the usefulness of our enzyme immunoassay, we have obtained the standard dose response curve of T$_4$ in patient's sera which is inversely proportional to the amount of herseradish peroxidase (HRP) conjugated monoclonal antibody of T$_4$ bound to the wells. The correlation coefficient (r) between the ELISA and chemiluminescent assay was 0.444 (n=38). Thus we have investigated the establishment of rapid and sensitive competitive ELISA assay method for detection of T$_4$ in patient's sera.

• Journal of Korean Society for Geospatial Information Science
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• v.7 no.2 s.14
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• pp.47-56
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• 1999

In this study, tie point observation in aerial triangulation was automated by the image processing methods. The technique includes boundary extraction and We matching processes. The procedures were applied to extract points of Interest and to find their conjugate points in the other images. The image coordinates of the identified points were then used to compute their absolute coordinates. An algorithm was developed in this study for the automation of observation in aerial triangulation, which is a manual process of selecting a tie point and recording the image coordinate of the selected point. The developed algorithm automates this process through the application of a mathematical operator to extract points of interest from an arbitrary image. The root m square error of image coordinates of the developed algorithm is $6.8{\mu}m$, which is close to that of the present analytical method. In a manual environment, the accuracy of the result of a photogrammetric process is heavily dependant on the level of skill and experience of the human operator. No such problem exists in an automated system. Also, as a result of the automated system, the time spent in the observation process could be reduced by a factor of 61.2%, thereby reducing the overall cost.

• Journal of Digital Contents Society
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• v.10 no.4
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• pp.579-585
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• 2009

In [9], Tyrtshnikov proposed a preconditioned approach to derive a general solution from a Toeplitz linear system. Furthermore, the process of selecting a preconditioner matrix from symmetric Toeplitz matrix, which has been used in previous studies, is introduced. This research introduces a new method for finding the preconditioner in a Toeplitz system. Also, through analyzing these preconditioners, it is derived that eigenvalues of a symmetric Toeplitz are very close to eigenvalues of a new preconditioner for T. It is shown that if the spectrum of the preconditioned system $C_0^{-1}T$ is clustered around 1, then the convergence rate of the preconditioned system is superlinear. From these results, it is determined to get the superliner at the convergence rate by our good preconditioner $C_0$. Moreover, an advantage is driven by increasing various applications i. e. image processing, signal processing, etc. in this study from the proposed preconditioners for Toeplitz matrices. Another characteristic, which this research holds, is that the preconditioner retains the properties of the Toeplitz matrix.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.34-34
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• 2017

Various extracts of roots, stems, and leaves of Phalaenopsis Sogo Yukidian 'V3' were evaluated for total phenolics, total flavonoids, and antioxidant capacity. The conjugate form of stem samples contained the highest total phenolics ($5.092{\pm}0.739mg$ gallic acid equivalent per g dry weight） and the highest total flavonoids ($2.218{\pm}0.021mg$ rutin equivalent per g dry weight) was found in the hexane extract of leaves. The ethyl acetate extract of roots showed the maximum antioxidant activity as compared to other extracts. Of which, the $IC_{50}$ value of this sample were 0.070 mg/mL and 0.450 mg/mL in the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and reducing power, respectively, while the lipid peroxidation inhibition (LPI) value was as 94.2% by ${\beta}$-carotene bleaching method. Five phenolic compounds including caffeic acid, syringic acid, vanillin, ellagic acid, and cinnamic acid were quantified by high-performance liquid chromatography (HPLC). It is suggested that roots of the hybrid Phalaenopsis Sogo Yukidian 'V3' can be explodited as an effective source of antioxidants.

• Journal of Microbiology and Biotechnology
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• v.19 no.1
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• pp.83-92
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• 2009

Individual immunochromatographic assays (ICG) for ochratoxin A (OTA) and zearalenone (ZEA) were optimized and used in the development of a one-step simultaneous immunochromatographic assay (OS-ICG) for the rapid multianalysis of two mycotoxins in corn samples. The nitrocellulose membrane of the OS-ICG was treated with OTA-bovine serum albumin (BSA), ZEA-ovalbumin (OVA), and anti-mouse IgG in the OTA test, ZEA test, and control zones, respectively. Monoclonal antibody-gold conjugates (OTA3 MAb-gold and ZEA2C5 MAb-gold) were sprayed onto the conjugate pad. The visual detection limits were 2.5 and 5 ng/ml for OTA and ZEA, respectively, and the results were obtained within 15 min after starting the analysis. An efficient, simple, and rapid extraction method using 30% MeOH/PBS was established and validated by analyzing the corn samples spiked with OTA/ZEA mixtures (0/0, 5/10, 10/20, and $20/30\;{\mu}g/kg$). The cut-off values of the OS-ICG for the spiked corn were 5 and $10\;{\mu}g/kg$ for OTA and ZEA, respectively. Natural corn samples were analyzed by OS-ICG, direct competitive enzyme-linked immunosorbent assay (DC-ELISA), and HPLC. Results of the OS-ICG were in good agreement with those obtained by DC-ELISA and HPLC. The developed OS-ICG offers a rapid, easy-to-use, and portable analytical system and can be used as a convenient qualitative tool for the on-site simultaneous determination of OTA and ZEA in cereals, food, and agricultural products in one analytical cycle.

• The Journal of the Korean Society for Microbiology
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• v.22 no.4
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• pp.377-392
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• 1987

Monoclonal antibody to the Escherichia coli heat-stable enterotoxin(ST) was produced to develop a rapid and convenient diagnostic method to the ST. The toxin was purified from culture supernatant of enterotoxigenic E. coli O148H28($ST^+/LT^+$) and conjugated to bovine serum albumin(BSA). The ST-BSA conjugate was used to immunize BALB/c mice and the immune spleen cells from these mice were fused with $P3{\times}63$ Ag8.V653 plasmacytoma cells. Hybridomas were screened by ELISA and positive hybridomas were cloned by limiting dilution. Finally, one stable clone (AS36) specific to ST was selected for further growth and characterization. Antibody titers of culture supernatant and ascitic fluid from BALB/c mice were 1:1,024 and 1:20,480 respectively in ELISA. The isotype and subclass of monoclonal antibody was IgG1 in sandwich ELISA. To test the neutralizing effect of monoclonal antibody on toxin activity of ST, mixture of ascitic fluid and ST was assayed by infant mouse assay and this monoclonel antibody was proved to be a neutralizing antibody. The titer of ascitic fluid which completely neutralized biological activity of 4 units of ST was 1:4. Purified ST was quantitatively measured by competitive ELISA and minimum amount of ST detectable by this assay was 250pg, which was an amount six-fold smaller than that detectable by infant mouse assay. Four reference strains of enterotoxigenic E. coli from WHO were detected by competitive ELISA and highly specific, sensitive and reproducible result was obtained.