• Journal of Pharmaceutical Investigation
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• 제29권4호
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• pp.287-293
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• 1999

Transport study of horseradish peroxidase and transferrin-horseradish peroxidase conjugate was performed using an in vitro Caco-2 cell cultured monolayer grown on a polycarbonate membrane of $Transwell^{\circledR}$, Horseradish peroxidase was not transported across Caco-2 cell monolayer. Transferrin-horseradish peroxidase conjugate was transported through Caco-2 cell monolayer. The apparent membrane permeability coefficient $(P_{app})$ of transferrin horseradish peroxidase conjugate was $6.54{\times}10^{-7}\;cm/sec$. The $P_{app}$ value of transferrin-horseradish peroxidase conjugate across Caco-2 cell monolayer was increased to $11.9{\times}10^{-7}\;cm/sec$ in the presence of $50\;{mu}g/ml$ brefeldin-A. These results suggest the transferrin receptor mediated transcytosis of transferrin-horseradish peroxidase conjugate across Caco-2 cell monolayer.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2004년도 제22회 춘계학술대회논문집
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• pp.831-838
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• 2004

A fluid-solid conjugate solver has been newly developed and applied to an actual engine disk system. Most of the currently available conjugate solvers lack the special thermal modeling for turbomachinery disk system applications. In the present new code, these special models are implemented to expand the applicability of the conjugate method and to reduce the required computational resources. Most of the conjugate analysis work so far are limited to the axisymmetric framework. However, the actual disk system includes several non-axisymmetric components which inevitably affect the local heat transfer phenomena. Also the previous work devoted to this area usually concentrate their efforts on the steady-state thermal field, although the one in the transient condition is more critical to the engine components. This paper presents full 3D conjugate analysis of a single stage high pressure turbine rotor-stator disk system to assess the three-dimensional effects (Fig. 1). The analysis is carried out not only in the steady-state but also in the engine accelerating transient condition. The predicted temperatures shows good agreement with measured data.

• 대한기계학회논문집B
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• 제30권3호
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• pp.238-245
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• 2006

This paper proposes appropriate conjugate parameters and dimensionless temperatures to analysis the conjugate problem of heat conduction in solid wall coupled with laminar film condensation flow adjacent to horizontal flat plate. An efficient methods for some fluids are proposed for its solution. The momentum and energy balance equations are reduced to a nonlinear system of ordinary differential equations with four parameters: the Prandtl number, Pr, Modified Jacob number, $Ja^*/Pr$, defined by an overall temperature difference, a property ratio $\sqrt{\rho_l{\mu}_l/{\rho_v{\mu}_v}$ and the conjugate parameter $\zeta$. The obtained similarity solution reveals the effect of the conjugate parameter, and the results are compared with the simplified solution. The variations of the heat transfer rates as well as the interface temperature and frictions along the plate are shown explicitly.

• Journal of Pharmaceutical Investigation
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• 제29권3호
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• pp.211-216
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• 1999

To evaluate the thrombolytic activity of streptokinase-dextran conjugate, a rat model of arterial thrombosis was used. Briefly, the femoral artery was exposed and a filter paper saturated with 70% $FeCl_3$ solution was placed around the femoral artery in order to stop the blood flow. Six minutes after the stop of the blood flow in the femoral artery, streptokinase $(10000{\sim}30000\;units\;per\;rat)$ or streptokinase-dextran conjugate $(5000{\sim}17000\;units\;per\;rat)$ was administered by i.v. bolus injection through the femoral vein. Then the blood flow in the femoral artery was monitored using a Doppler laser flow meter. The i.v. bolus administration of streptokinase could not restore the blood flow in the femoral artery in the dose range of $10000{\sim}30000$ units per rat. The i.v. bolus administration of streptokinase-dextran conjugate could restore the blood flow in the femoral artery in the dose range of $5000{\sim}17000$ units per rat. A good correlation between the dose of streptokinase-dextran conjugate and the total thrombolytic effect was observed. In addition, the lag time between the injection of streptokinase-dextran conjugate and the restoring of the blood flow was decreased as the i.v. dose of streptokinase dextran conjugate increased. These results show the superior beneficial effect of streptokinase-dextran conjugate compared with the unconjugated streptokinase with respect to the elongation of thrombolytic activity, the administration method (single injection versus continuous infusion), and the reduced dose necessary for a equivalent thrombolytic effect.

• Biomolecules & Therapeutics
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• 제23권6호
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• pp.493-509
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• 2015

Antibody-drug conjugates utilize the antibody as a delivery vehicle for highly potent cytotoxic molecules with specificity for tumor-associated antigens for cancer therapy. Critical parameters that govern successful antibody-drug conjugate development for clinical use include the selection of the tumor target antigen, the antibody against the target, the cytotoxic molecule, the linker bridging the cytotoxic molecule and the antibody, and the conjugation chemistry used for the attachment of the cytotoxic molecule to the antibody. Advancements in these core antibody-drug conjugate technology are reflected by recent approval of Adectris$^{(R)}$(anti-CD30-drug conjugate) and Kadcyla$^{(R)}$(anti-HER2 drug conjugate). The potential approval of an anti-CD22 conjugate and promising new clinical data for anti-CD19 and anti-CD33 conjugates are additional advancements. Enrichment of antibody-drug conjugates with newly developed potent cytotoxic molecules and linkers are also in the pipeline for various tumor targets. However, the complexity of antibody-drug conjugate components, conjugation methods, and off-target toxicities still pose challenges for the strategic design of antibody-drug conjugates to achieve their fullest therapeutic potential. This review will discuss the emergence of clinical antibody-drug conjugates, current trends in optimization strategies, and recent study results for antibody-drug conjugates that have incorporated the latest optimization strategies. Future challenges and perspectives toward making antibody-drug conjugates more amendable for broader disease indications are also discussed.

• 한국기계가공학회지
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• 제2권2호
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• pp.52-59
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• 2003

Cam mechanism is a machine part frequently used in machinery. Specially, conjugate cam mechanism is very suitable for the high speed working and the heavy power translation. Then a conjugate cam mechanism need high precision for the relations between cam profiles and follower rollers. So, its design and manufacturing are very difficult. Thus, this study is a branch of exclusive CAM systems for design and NC machining of conjugate earn mechanism based on a master plate earn profile in order to exchange an old plate cam mechanism to a new conjugate earn mechanism. For the design of the other cam profile by using a master cam profile, some calculation processes were used by vector summation methods, from master cam profile data to the center data of master follower, from the center data of master follower to the center data of the other follower considered in link mechanism, and offsetting in the center direction of base circle of the other cam from the center data of the other follower. Finally, a sample conjugate cam was selected and machined m order to prove the contents of this study.

• Journal of Microbiology and Biotechnology
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• 제20권10호
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• pp.1424-1429
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• 2010

A poly(${\gamma}$-glutamic acid) (${\gamma}$PGA)-cholesterol conjugate was synthesized and its properties were then evaluated. The conjugate exhibited an amphiphilic nature derived from the hydrophilic ${\gamma}$PGA backbone and the hydrophobic cholesterol side chain. The conjugate spontaneously formed nanoparticles, becoming an aqueous solution when at low concentrations, and at high concentrations the result was the formation of a physical gel. By utilizing the self-aggregating properties of the conjugate in water, an artificial chaperone was developed. A complex of protein, with the nanoparticles of the conjugate, was formed and the protein was released upon the dissociation of the nanoparticles through the addition of ${\beta}$-cyclodextrin. For denatured carbonic anhydrase, the activity was recovered in the artificial chaperone of the nanoparticle conjugate.

• Journal of applied mathematics & informatics
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• 제29권5_6호
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• pp.1303-1309
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• 2011

Based on the PRP method, a new spectral PRP conjugate gradient method has been proposed to solve general unconstrained optimization problems which produce sufficient descent search direction at every iteration without any line search. Under the Wolfe line search, we prove the global convergence of the new method for general nonconvex functions. The numerical results show that the new method is efficient for the given test problems.

• 생약학회지
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• 제19권2호
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• pp.127-132
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• 1988

In the course of the immunological studies on the natural products, the antibody specific to saikosaponin a was producted. Saikosaponin a was treated with succinic anhydride to give 6'-O-hemisuccinyl saikosaponin a, which was successively converted to saikosaponin a-BSA conjugate (4. 5 mole saikosaponin a/mole of BSA) by carbodiimide method. The antibody obtained from rabbits immunized with saikosaponin a-BSA conjugate as usual manner reacted with both the conjugate and BSA, while after the absorption with BSA, the antibody reacted with the conjugate alone.

• 충청수학회지
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• 제11권1호
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• pp.129-142
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• 1998

In this paper, we propose a blocked variant of the Conjugate Gradient method which performs as well as and has coarser parallelism than the classical Conjugate Gradient method.