• Journal of Microbiology and Biotechnology
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• 제31권4호
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• pp.501-509
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• 2021

More than half the world's population is thought to be infected with Helicobacter pylori. Although the majority of infected people are asymptomatic, H. pylori infection may cause gastric ulcers and deadly gastric cancer. Owing to the difficulty and invasiveness of current routine culture and diagnostic methods, a highly sensitive and specific noninvasive assay for H. pylori is of interest. This study highlighted the design and performance of a colorimetric magneto loop-mediated isothermal amplification (CM-LAMP) assay to detect H. pylori in spiked saliva samples. LF primers were coated on magnetic nanoparticles by carbodiimide-induced immobilization and functionally used for solid-phase amplification. During the LAMP reaction at 66℃, biotin-tagged FIPs were incorporated into LAMP amplicons. The colorimetric signal developed after the addition of NeutrAvidin horseradish peroxidase conjugate (NA-HRP) and ABTS. None of the tested microorganisms, including closely related bacteria, was shown positive by the CM-LAMP assay except H. pylori isolates. This novel platform was highly specific and 100-fold more sensitive (40 CFU/ml or 0.2 CFU per reaction) than the PCR and conventional LAMP assays for the detection of H. pylori in spiked saliva. Our results demonstrated the feasibility of using this noninvasive molecular diagnostic test to detect H. pylori in saliva samples.

• Annals of Surgical Treatment and Research
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• 제95권5호
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• pp.249-257
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• 2018

Purpose: Multidrug resistance-associated protein (MRP) 2 is a glutathione conjugate in the canalicular membrane of hepatocytes. Early graft damage after liver transplantation (LT) can result in alteration of MRP2 expression. The purpose of this study was to evaluate the relationship between the pattern of MRP2 alteration and graft outcome. Methods: Forty-one paraffin-embedded liver graft tissues obtained by protocol biopsy within 2 months after LT; these were stained using monoclonal antibodies of MRP2. We selected 15 live donor biopsy samples as a control, that showed homogenous canalicular staining for MRP2. The pattern of canalicular MRP2 staining of graft was classified into 3 types: homogenous (type C0), focal (type C1), and no (type C2,) staining of the canaliculi. Results: In total, 17.1% graft tissues were type C0, 36.6% were type C1, and 46.3% were type C2. The median operation time was longer in patients with type C2 (562.6 minutes) than in patients with type C0 (393.8 minutes) (P = 0.038). The rates of posttransplant complications were higher in patients with type C2 (100%) than in patients with type C0 (42.9%) and C1 (73.3%) (P < 0.001). Conclusion: MRP2 expression pattern was altered in 82.9% after LT. The pattern of MRP2 alteration was associated with longer operation time and higher rates of post-LT complications.

• 한국산학기술학회논문지
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• 제20권7호
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• pp.423-429
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• 2019

최근에 초고속 광대역 무선통신 전송 시스템의 수신 성능 개선을 위한 다양한 기술들이 제안되고 있다. 본 논문에서는 주파수 공간 블록 부호화된 단일 측대 파형 SC (Single Carrier) FDMA (Frequency Division Multiple Access) 전송 시스템을 제안한다. 본 논문에서 제안된 주파수 공간 블록 부호화된 단일 측대 파형 SC FDMA 전송 시스템에서는 PAM (Pulse Amplitude Modulation) 신호의 DFT (Discrete Fourier Transform) 확산에 따라 생성되는 켤레 복소수 대칭을 활용하여 주파수 공간 블록 부호를 구현한다. 이를 통해 시스템 계산 복잡도를 크게 증가시키지 않으면서 단일 측대 파형 SC FDMA 전송 시스템의 성능 개선을 위한 송신 다이버시티 이득을 획득할 수 있다. 본 논문에서 제안된 주파수 공간 블록 부호화 단일 측대 파형 SC FDMA 시스템의 신호 대 잡음 비 성능이 단일 안테나 기반의 단일 측대 파형 SC FDMA 시스템보다 $10^{-2}$ 심볼 오류율 수준에서 약 4 dB 이상 개선되는 것을 실험을 통해 확인할 수 있다.

• 한국측량학회지
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• 제38권6호
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• pp.655-661
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• 2020

SGM (Semi Global Matching)은 입체영상 간 모든점의 매칭점을 찾기 때문에 고해상도 위성영상으로부터 고밀도 수치표면모델 제작이 가능하다. 그러나 물과 그림자, 폐색 지역이 포함되면 그 주변지점에도 오매칭의 영향을 준다. 특히 우리나라 아파트 구조물과 같이 시차가 크고 길쭉한 형태의 건물에서는 50cm급 고해상도 위성영상에 SGM 방법을 적용하더라도 건물의 3차원 복원은 어렵다. 따라서 본 연구는 1m급 해상도의 IKONOS-2 입체 위성 영상으로부터 분류영상과 에지영상을 이용한 SGM 기법을 제안, 적용하여 수치표면모델을 제작하고 일반 SGM 방법, ERDAS 소프트웨어의 고밀도 ABM (Area Based Matching) 매칭으로 제작한 수치표면모델과 비교하였다. 실험 대상지역에는 제안방법의 아파트 수치표면모델 결과가 가장 우수하였다. 결과적으로, 해상도가 1m 임에도 불구하고 제안방법을 이용하여 건물 수치표면모델의 윤곽선을 기존 방법에 비해 더욱 더 선명하게 표현할 수 있었다.

• 대한의용생체공학회:의공학회지
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• 제42권3호
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• pp.125-142
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• 2021

The POCT (point-of-care test) sensing that has been a fast-developing field is expected to be a next generation technology in health care. The POCT sensors for the detection of proteins, small molecules and especially nucleic acids have lately attracted considerable attention. According to the World Health Organization (WHO), the POCT methods are required to follow the ASSURED guidelines (Affordable, Sensitive, Specific, User- friendly, Robust and rapid, Equipment-free, Deliverable to all people who need the test). Recently, several CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) based diagnostic techniques using the sensitive gene recognition function of CRISPR have been reported. CRISPR/Cas (Cas, CRISPR associated protein) systems based detection technology is the most innovative gene analysis technology that is following the ASSURED guidelines. It is being re-emerged as a powerful diagnostic tool that can detect nucleic acids due to its characteristics that enable rapid, sensitive and specific analyses of nucleic acid. The first CRISPR-based diagnosis begins with the discovery of the additional function of Cas13a. The enzymatic cleavage occurs when the conjugate of Cas protein and CRISPR RNA (crRNA) detect a specific complementary sequence of the target sequence. Enzymatic cleavage occurs on not only the target sequence, but also all surrounding non-target single-stranded RNAs. This discovery was immediately utilized as a biosensor, and numerous sensor studies using CRISPR have been reported since then. In this review, the concept of CRISPR, the characteristics of the Cas protein required for CRISPR diagnosis, the current research trends of CRISPR diagnostic technology, and some aspects to be improved in the future are covered.

• Animal Bioscience
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• 제35권7호
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• pp.1091-1099
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• 2022

Objective: The aim of this work was the characterization of the qualitative aspects of meat obtained from Teramana goats, an Italian indigenous breed of the Abruzzo region. Specifically, the study included a comparison with meat samples deriving from Saanen goat kids reared in the same environment and applying the same feeding protocol. Methods: Upon reaching about 7 months of age the animals were slaughtered and samples of muscle tissue were collected to be analyzed. Specifically, meat samples were subjected to evaluations of the physical parameters, including color and the meat ability to retain water, in addition to chemical evaluations that were focused to the determination of the total lipids amount, fatty acids composition, lipid oxidation, and volatile profile. Results: The meat samples obtained from the indigenous breed showed a less intense reddish color and no significant variations for the muscle tissue tendency to retain water, both regarding fresh and cooked meat. Several differences were instead observed in the fatty acid profile. The Teramana samples were richer in saturated fatty acids (p<0.01) and interestingly showed higher concentrations of rumenic acid (p<0.05), a conjugate of linoleic acid that has been associated with important health benefits for the consumers. Another important finding for these meat samples was the marked resistance to oxidative events, as evidenced by the thiobarbituric acid reactive substances-test (p<0.05) and by the characterization of the volatile profile that highlighted a strong reduction in the relative percentage of hexanal (p<0.05), commonly associated to lipid oxidation and the development of unpleasant aromatic notes. Conclusion: The collected data, therefore appeared useful for the valorization of the food product derived from the Teramana goat, although no sensory information has been collected to define the degree of acceptability by the consumers.

• 한국산업정보학회논문지
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• 제26권6호
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• pp.1-9
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• 2021

주파수 전송 효율을 높이기 위하여 주파수 대역에서의 켤레 복소수 대칭 특성을 갖는 단일 측대 파형 전송 시스템의 연구가 이루어지고 있다. 또한, 직교 블록 부호(시공간 및 주파수 공간 블록 부호)를 활용한 전송 시스템의 다이버시티 성능 이득에 대하여 많은 연구가 진행되어 왔다. 본 논문에서는 4개의 송신 안테나를 갖는 3/4 비율의 주파수 공간 블록 부호 단일 측대 파형 SC(single carrier) FDMA(frequency division multiple access) 시스템을 구현한다. 3/4 비율의 4×4 직교 블록 부호를 적용함으로써 기존의 단일 안테나 기반 단일 측대 파형 SC FDMA 시스템과 2개의 송신 안테나를 이용한 2×2 주파수 공간 블록 부호 단일 측대 파형 SC FDMA 시스템보다 뛰어난 수신 성능을 확보할 수 있음을 실험 결과를 통해 확인할 수 있다.

• Animal Bioscience
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• 제35권1호
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• pp.105-114
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• 2022

Objective: This work was carried out to evaluate the effect of pasture (PA) feeding on buffalo meat quality compared with buffaloes reared intensively with the use of corn silage as a forage base or alternatively with polyphite meadow hay (PH). Methods: Thirty Mediterranean bull buffaloes were distributed into three experimental diet groups: maize silage (MS), PH, and PA. The animals were slaughtered at a live weight of 250 kg, and carcass and meat quality were evaluated. After 7 days of ageing, physical and chemical parameters of longissimus thoracis muscle were determined. To evaluate lipid oxidation the thiobarbituric acid reactive substances was tested at 7 and 14 days, and also the fatty acid profile was recorded by gas chromatography. Results: The PA group, even if it showed carcass parameters lower than those of the silage maize group, reported a good meat percentage (60.59% vs 58.46%, respectively) and lower fat percentage (p<0.001). PA-fed animals showed meat redness, and even if only on raw meat, shear force was higher than the others. Low values of conjugate linoleic acid, polyunsaturated fatty acids, and n-3 were reported in the silage maize group. Principal component analysis (PCA) clearly showed the influence of different diets on meat quality, and PCA1 and PCA2 explained 82% of the variability. Conclusion: Buffaloes reared on PA had meat with high nutritional value even if they showed poor carcass performance compared to the animals fed on MS. Buffaloes fed on polyphite hay were in an intermediate position, similar to grazing animals, according to the same nutritional determinations.

• Journal of Dairy Science and Biotechnology
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• 제40권4호
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• pp.143-150
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• 2022

본 연구에서는 분리유청단백질과 이눌린을 이용하여 마이야르 공액체를 성공적으로 제조하였다. 제조된 마이야르 공액체의 제조공정요인인 pH와 열처리 온도는 마이야르 공액체의 기능적 특성(유화특성, 표면특성, 항산화특성)에 영향을 미치는 핵심 제조공정임을 알 수 있었으며, 상대적으로 마이야르 반응이 활발한 pH 8.4와 열처리 온도 70℃에서 마이야르 공액체의 기능적 특성이 향상되었음을 확인하였다.

• 한국측량학회지
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• 제39권6호
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• pp.533-540
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• 2021

고해상도 위성영상은 극지 내륙 지역과 같이 접근이 어려운 지역에 대해서도 공간 정보를 획득할 수 있는 탁월한 접근성을 가진다. 고해상도 위성영상으로부터 도출되는 공간정보의 위치 정확도를 향상시키기 위해서는 기준점을 활용하는데, 이러한 접근 불가 지역에 대해서는 기준점 획득이 쉽지 않기 때문에 여러 보조 데이터를 쓰기도 하나, 그러한 보조 데이터 마저 획득이 어려운 지역이 존재한다. 따라서 본 논문에서는 완전한 무기준점 기반으로 위치 정확도를 향상시키기 위한 방법으로 멀티 영상의 번들조정을 기반으로 정확도 향상의 정도를 평가하였다. 멀티 영상 조정을 위해 영상 간의 매칭점를 추출하여 활용하였고, 개별 영상 또는 스테레오 영상의 조정이 아닌 전체 영상의 통합 센서 모델링을 구현하여 정확도 향상 정도를 평가하였다. 실험으로 아리랑 3A 영상을 활용하였으며, 실험결과 RMSE (Root Mean Square Error) 오차의 현격한 향상은 도출하기 어려웠으나, 최대오차를 감소시키는 효과가 있었으며, 특히 표고 방향으로의 과대오차를 감소시키는데 효과적임을 알 수 있었다.