• Molecules and Cells
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• 제21권2호
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• pp.308-313
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• 2006

Amygdalin is a cyanogenic glycoside compound which is commonly found in the pits of many fruits and raw nuts. Although amygdalin itself is not toxic, it can release cyanide (CN) after hydrolysis when the pits and nuts are crushed, moistened and incubated, possibly within the gastrointestinal tract. CN reversibly inhibits cellular oxidizing enzymes and cyanide poisoning generates a range of clinical symptoms. As some pits and nuts may contain unusually high levels of amygdalin such that there is a sufficient amount to induce critical CN poisoning in humans, the detection of abnormal content of amygdalin in those pits and nuts can be a life-saving measure. Although there are various methods to detect amygdalin in food extracts, an enzyme immunoassay has not been developed for this purpose. In this study we immunized New Zealand White rabbits with an amygdalin-KLH (keyhole limpet hemocyanin) conjugate and succeeded in raising anti-sera reactive to amygdalin, proving that amygdalin can behave as a hapten in rabbits. Using this polyclonal antibody, we developed a competition enzyme immunoassay for determination of amygdalin concentration in aqueous solutions. This technique was able to effectively detect abnormally high amygdalin content in various seeds and nuts. In conclusion, we proved that enzyme immunoassay can be used to determine the amount of amygdalin in food extracts, which will allow automated analysis with high throughput.

• Journal of Animal Science and Technology
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• 제47권1호
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• pp.19-28
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• 2005

The objectives of the current study were to develop polyclonal antibodies in sheep against adipocyte plasma membrane(APM) proteins isolated from swine, to investigate tissue specificity, and to determine cytotoxic effects of antiserum on swine adipocytes. Plasma membrane proteins from adipocyte, brain, heart, kidney, liver, and spleen were isolated using a 32% sucrose gradient. Adult male sheep was immunized three times at three week interval with the purified swine APM proteins. Antiserum was taken from immunized sheep at 10, 12, and 14 days after the third immunization. Antiserum expressed strong reactivity with APM proteins determined by enzyme-linked immunosorbent assay(ELISA), and the reactivity could be detected at dilutions in excess of 1 : 81,000. Antiserum showed very low binding affinity with proteins isolated from brain, heart, kidney, liver, or spleen. Tissue specificity of the antiserum was reconfirmed by Western immunoblotting using anti-sheep immunoglobulin G•alkalinephosphatase conjugate as a secondary antibody. The reactivity of antiserum to the external surface of fixed swine adipocytes was confmned by an immunohistochemical technique using anti-sheep immunoglobulin G-FITC. Confluent swine adipocytes in culture were lysed by antiserum treatment and cytosolie lactate dehydrogenase(LDH) was released as a dose-dependent patterns while adipocytes treated with normal sheep serum maintained their integrity and expressed low level of LDH. These results implicate that fat contents in the pigs can be reduced by immunological methods.

• The Korean Journal of Physiology and Pharmacology
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• 제19권4호
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• pp.319-325
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• 2015

Among solute carrier proteins, the organic anion transporters (OATs) play an important role for the elimination or reabsorption of endogenous and exogenous negatively charged anionic compounds. Among OATs, SLC22A9 (hOAT7) transports estrone sulfate with high affinity. The net decrease of estrogen, especially in post-menopausal women induces rapid bone loss. The present study was performed to search the SNP within exon regions of SLC22A9 in Korean females with osteoporosis. Fifty healthy controls and 50 osteoporosis patients were screened for the genetic polymorphism in the coding region of SLC22A9 using GC-clamped PCR and denaturing gradient gel electrophoresis (DGGE). Six SNPs were found on the SLC22A9 gene from Korean women with/without osteoporosis. The SNPs were located as follows: two SNPs in the osteoporosis group (A645G and T1277C), three SNPs in the control group (G1449T, C1467T and C1487T) and one SNP in both the osteoporosis and control groups (G767A). The G767A, T1277C and C1487T SNPs result in an amino acid substitution, from synonymous vs nonsynonymous substitution arginine to glutamine (R256Q), phenylalanine to serine (F426S) and proline to leucine (P496L), respectively. The Km values and Vmax of the wild type, R256Q, P496L and F426S were 8.84, 8.87, 9.83 and $12.74{\mu}M$, and 1.97, 1.96, 2.06 and 1.55 pmol/oocyte/h, respectively. The present study demonstrates that the SLC22A9 variant F426S is causing inter-individual variation that is leading to the differences in transport of the steroid sulfate conjugate (estrone sulfate) and, therefore this could be used as a marker for certain disease including osteoporosis.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2006년도 춘계종합학술대회
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• pp.361-364
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• 2006

광 전송로로 비영 분산천이 광섬유 (NZ-DSF ; Non Zero - Dispersion Shifted Fiber)를 이용한 $8\times40$ Gbps WDM 시스템에서 모든 채널을 효과적으로 보상할 수 있는 광 위상 공액기 (OPC ; Optical Phase Conjugate.)의 최적 위치와 광섬유의 최적 분산 계수 값을 도출하는 수치적 방법을 제안하였다. 이 방법의 유용성을 확인하기 위하여 도출된 두 최적 파라미터를 갖는 시스템에서의 비트 에러율 (BER ; Bit Error Rate) 특성을 현재 일반화된 MSSI (Mid-Span Spectral Inversion)에서의 BER 특성과 비교하였다. 최적 파라미터들을 갖는 OPC를 WDM 시스템에 적용하게 되면 채널 간 파워 패널티를 4배 이상으로 감소시킬 수 있다는 것을 알 수 있었다.

• 터널과지하공간
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• 제17권2호
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• pp.119-127
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• 2007

임계면법을 적용하여 횡등방성 암석의 강도이방성을 해석하는 방법을 제안하였다. 암석의 파괴는 Hoek-Brown 파괴기준을 따르는 것으로 가정하였다. Hoek-Brown의 경험적 파괴기준식에 대응되는 Mohr 포착선식을 이용하고 강도상수인 m과 s를 방향에 따른 스칼라 함수로 정의하여 이방성 파괴함수를 구성하였다. 이방성 파괴함수를 최대고 하는 임계면의 방향을 찾기 위하여 직접 최적화기법의 하나인 공액구배법을 적용하였다. 횡등방성 안석에 대한 기존 이방성 강도모델이 대부분 삼축압축실험과 동일한 응력조건에서만 적용할 수 있는데 반하여 이 연구에서 제안된 방법은 일반적인 3차원 응력조건에도 쉽게 적용할 수 있다는 장점을 가지고 있다. 삼축압축실험의 모사를 통하여 얻어진 삼축압축강도와 파괴면의 경사에 분석을 통하여 제안된 방법의 적합성을 검토하였다.

• 한국식품과학회지
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• 제32권5호
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• pp.1009-1014
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• 2000

PA를 분석하기 위하여 효소면역측정법을 개발하고자 하였다. Bc방법과 Po방법으로 BSA에 PA를 conjugation하여 각각의 PA-BSA conjugate(PA-BSA[Bc]와 PA-BSA[Po])를 제조하였으며, 이를 토끼에 면역하여 항PA-BSA 항체를 얻었다. 항PA-BSA[Po] 항체를 사용한 ciELISA의 결과에서 경합반응이 제대로 일어나지 않았기 때문에, 식품 속에 있는 PA를 검출하기 위해서 PA-BSA[Po]을 코팅한 후 항PA-BSA[Bc] 항체를 사용하였다. 이 결과에서 PA의 검출한계가 1 ppm인 것을 확인할 수 있었으며, 교차반응을 통해 PA 유도체들에 대해서도 항PA-BSA[Bc] 항체가 PA에 대해 특이성이 매우 강하였다. 또한, MBA의 결과에서는 그 검출한계가 10ppb인 것을 확인할 수 있었다. 분석시료인 계란(109%), 상추(64%), 소간(344%)의 식품시료에 대한 실험에서 상추를 제외하고는 ciELISA는 MBA의 결과와 비교해 볼 때 양호한 결과를 얻을 수 있었다. 그러므로, ciELISA는 MBA보다 분석시간, 교차반응 등의 면에서 장점이 있어 식품 중 PA의 검출에 효과적으로 활용할 수 있을 것이다.

• 한국항해항만학회지
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• 제32권10호
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• pp.805-811
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• 2008

이 논문은 차량용 사이드 미러에 적합한 광대역 RFID 태그안테나를 설계하고, 차체에 의한 지향성의 특성을 제시한다. 제안된 태그 안테나는 인식거리와 광대역 특성을 향상시키기 위해 대칭 구조로 설계되었다 제안된 태그 안테나($30\;mm{\times}24\;mm{\times}1\;mm$)는 910 MHz에서 공진하며 대역폭은 780 MHz($540\;MHz{\sim}1320\;MHz$)를 가진다. 칩 임피던스는 16 - $j131\;{\Omega}$이고, 상용 칩의 복소 공액 임피던스가 태그 안테나의 설계에 사용되었다. 제안된 태그 안테나는 차량의 사이드 미러의 내부에 위치한다. 사이드 미러의 유전율뿐만 아니라 차체(도체)에 대한 태그 안테나의 효과를 평가하기 위해, 지향패턴 측정과 인식거리가 계산되고 측정되었다. 차량용 RFID 시스템을 위한 최적의 위치가 사이드 미러 내부에서 관측되었으며, 계산된 결과들은 측정된 결과들과 잘 일치하였다.

• 약학회지
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• 제57권1호
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• pp.1-7
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• 2013

Induction of effective and increased levels of antibody production may be major points in vaccine development. This is especially the case when the antigenic sources are carbohydrates. Thus, in our Lab various types of formulations such as liposomal and conjugate vaccines have been researched. However, the fastidious formulation process and high costs are a problem. For this reason, there is currently a focus on utilizing immunoadjuvants. In this present study, we tested whether ginsenosides Re (a panaxdiol) and Rg1 (a panaxtriol) from Panax ginseng have immunoadjuvant activity against the cell wall of Candida albicans (CACW). The resulting data showed that Rd and Rg1 caused LPS-treated B lymphocytes to be proliferative. Rd had greater proliferation activity than that of Rg1. In the murine model of antibody production, CACW combined with Rd [CACW/Rd/IFA] or Rg1 [CACW/Rg1/IFA] increased the production of antibodies specific to C. albicans when compared to the antibody production by [CACW/IFA]-induction, which was used as a negative control (P<0.05). In the case of [CFA/Rd/IFA], the antibody production was almost twice as that of the CFA. In addition, formulations containing either had a prolonged antibody inducing activity as compared to the CFA formula. In conclusion, Rd and Rg1 have an immunologic activity, and yet Rd can be a better candidate than Rg1 for a new immunoadjuvant.

• Tribology and Lubricants
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• 제37권4호
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• pp.117-124
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• 2021

In this study, we perform a 3D CFD conjugate analysis according to the shape of the foil ramp of the air foil thrust bearing, analyze the flow characteristics inside the bearing, and compare the results corresponding to the two shapes. Air has a lower viscosity than lubricating oil. Therefore, the thrust runner of the bearing must rotate at high speed to support the load. The gap between thrust runner and foil is significantly smaller than that of the oil bearing. Hence, it is crucial to analyze the complex flow characteristics inside the bearing to predict the complex flow inside the bearing and performance of the bearing. In addition, flow characteristics may appear differently depending on the ramp shape of the bearing foil, which may affect bearing performance. In this study, we numerically analyze the main flow path of air flowing into the bearing and the secondary flow path used for cooling the bearing using the commercial CFD software ANSYS CFX and compare the flow characteristics for straight and curved foil ramp shapes. Notably, there is a difference in the speed of the flowing air according to the shape of the ramp, which affects the bearing performance.

• Investigative Magnetic Resonance Imaging
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• 제25권3호
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• pp.141-155
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• 2021

Purpose: To develop a 3D magnetic resonance fingerprinting (MRF) method for application in high resolution knee cartilage PD, T₁, T₂ mapping. Materials and Methods: A novel 3D acquisition trajectory with golden-angle rotating radial in k_xy direction and interleaved echo planar imaging (EPI) acquisition in the k_z direction was implemented in the MRF framework. A centric order was applied to the interleaved EPI acquisition to reduce Nyquist ghosting artifact due to field inhomogeneity. For the reconstruction, singular value decomposition (SVD) compression method was used to accelerate reconstruction time and conjugate gradient sensitivity-encoding (CG-SENSE) was performed to overcome low SNR of the high resolution data. Phantom experiments were performed to verify the proposed method. In vivo experiments were performed on 6 healthy volunteers and 2 early osteoarthritis (OA) patients. Results: In the phantom experiments, the T₁ and T₂ values of the proposed method were in good agreement with the spin-echo references. The results from the in vivo scans showed high quality proton density (PD), T₁, T₂ map with EPI echo train length (N_ETL = 4), acceleration factor in through plane (R_z = 5), and number of radial spokes (N_spk = 4). In patients, high T₂ values (50-60 ms) were seen in all transverse, sagittal, and coronal views and the damaged cartilage regions were in agreement with the hyper-intensity regions shown on conventional turbo spin-echo (TSE) images. Conclusion: The proposed 3D MRF method can acquire high resolution (0.5 mm³) quantitative maps in practical scan time (~ 7 min and 10 sec) with full coverage of the knee (FOV: 160 × 160 × 120 mm³).