• Journal of Microbiology and Biotechnology
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• v.31 no.4
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• pp.501-509
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• 2021

More than half the world's population is thought to be infected with Helicobacter pylori. Although the majority of infected people are asymptomatic, H. pylori infection may cause gastric ulcers and deadly gastric cancer. Owing to the difficulty and invasiveness of current routine culture and diagnostic methods, a highly sensitive and specific noninvasive assay for H. pylori is of interest. This study highlighted the design and performance of a colorimetric magneto loop-mediated isothermal amplification (CM-LAMP) assay to detect H. pylori in spiked saliva samples. LF primers were coated on magnetic nanoparticles by carbodiimide-induced immobilization and functionally used for solid-phase amplification. During the LAMP reaction at 66℃, biotin-tagged FIPs were incorporated into LAMP amplicons. The colorimetric signal developed after the addition of NeutrAvidin horseradish peroxidase conjugate (NA-HRP) and ABTS. None of the tested microorganisms, including closely related bacteria, was shown positive by the CM-LAMP assay except H. pylori isolates. This novel platform was highly specific and 100-fold more sensitive (40 CFU/ml or 0.2 CFU per reaction) than the PCR and conventional LAMP assays for the detection of H. pylori in spiked saliva. Our results demonstrated the feasibility of using this noninvasive molecular diagnostic test to detect H. pylori in saliva samples.

• Annals of Surgical Treatment and Research
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• v.95 no.5
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• pp.249-257
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• 2018

Purpose: Multidrug resistance-associated protein (MRP) 2 is a glutathione conjugate in the canalicular membrane of hepatocytes. Early graft damage after liver transplantation (LT) can result in alteration of MRP2 expression. The purpose of this study was to evaluate the relationship between the pattern of MRP2 alteration and graft outcome. Methods: Forty-one paraffin-embedded liver graft tissues obtained by protocol biopsy within 2 months after LT; these were stained using monoclonal antibodies of MRP2. We selected 15 live donor biopsy samples as a control, that showed homogenous canalicular staining for MRP2. The pattern of canalicular MRP2 staining of graft was classified into 3 types: homogenous (type C0), focal (type C1), and no (type C2,) staining of the canaliculi. Results: In total, 17.1% graft tissues were type C0, 36.6% were type C1, and 46.3% were type C2. The median operation time was longer in patients with type C2 (562.6 minutes) than in patients with type C0 (393.8 minutes) (P = 0.038). The rates of posttransplant complications were higher in patients with type C2 (100%) than in patients with type C0 (42.9%) and C1 (73.3%) (P < 0.001). Conclusion: MRP2 expression pattern was altered in 82.9% after LT. The pattern of MRP2 alteration was associated with longer operation time and higher rates of post-LT complications.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.7
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• pp.423-429
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• 2019

Recently, a variety of methods for the performance improvement of ultra-high speed wideband wireless transmission systems have been suggested. This paper proposes a space-frequency (SF) block coded single side band (SSB) single carrier (SC)-frequency division multiple access (FDMA) transmission system. In the proposed SSB SC-FDMA system, SF block code is implemented with the complex conjugates, which are formed from discrete Fourier transform (DFT) spreading of pulse amplitude modulation (PAM) signals. As a result, transmit diversity gain can be obtained in the proposed SF block coded SSB SC-FDMA system without any significant increase of the system computational complexity. The simulation result shows that the signal-to-noise power ratio (SNR) performance of the proposed SF block coded SSB SC-FDMA system is approximately 4 dB better than the SNR performance of the conventional SSB SC-FDMA system with single transmit antenna at a symbol error rate (SER) of $10^{-2}$.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.38 no.6
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• pp.655-661
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• 2020

SGM (Semi Global Matching) can be used to find all the conjugate points between stereo images. Therefore, it enables high-density DSM (Digital Surface Model) production from high-resolution satellite images. However, water, shadows, and occlusion areas cause mismatching of the surrounding points in this method. Particularly, in buildings with large-parallax and elongated-shapes such as a Korean style apartment, it is difficult to reconstruct the 3D building even if the SGM method is applied to a high-resolution 50cm satellite image. This study proposed and performed the SGM technique with a classified image and an edge image from the IKONOS-2 satellite stereo-image with a 1m resolution to produce DSM. It was compared with the DSMs from the general SGM and the high-density ABM (Area Based Matching) matching of ERDAS software. The results of the apartment DSM by the proposed method were the best in the test area. As a result, despite the image having a resolution of 1m, the outline of the building DSM could be expressed more clearly than the existing method.

• Journal of Biomedical Engineering Research
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• v.42 no.3
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• pp.125-142
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• 2021

The POCT (point-of-care test) sensing that has been a fast-developing field is expected to be a next generation technology in health care. The POCT sensors for the detection of proteins, small molecules and especially nucleic acids have lately attracted considerable attention. According to the World Health Organization (WHO), the POCT methods are required to follow the ASSURED guidelines (Affordable, Sensitive, Specific, User- friendly, Robust and rapid, Equipment-free, Deliverable to all people who need the test). Recently, several CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) based diagnostic techniques using the sensitive gene recognition function of CRISPR have been reported. CRISPR/Cas (Cas, CRISPR associated protein) systems based detection technology is the most innovative gene analysis technology that is following the ASSURED guidelines. It is being re-emerged as a powerful diagnostic tool that can detect nucleic acids due to its characteristics that enable rapid, sensitive and specific analyses of nucleic acid. The first CRISPR-based diagnosis begins with the discovery of the additional function of Cas13a. The enzymatic cleavage occurs when the conjugate of Cas protein and CRISPR RNA (crRNA) detect a specific complementary sequence of the target sequence. Enzymatic cleavage occurs on not only the target sequence, but also all surrounding non-target single-stranded RNAs. This discovery was immediately utilized as a biosensor, and numerous sensor studies using CRISPR have been reported since then. In this review, the concept of CRISPR, the characteristics of the Cas protein required for CRISPR diagnosis, the current research trends of CRISPR diagnostic technology, and some aspects to be improved in the future are covered.

• Animal Bioscience
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• v.35 no.7
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• pp.1091-1099
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• 2022

Objective: The aim of this work was the characterization of the qualitative aspects of meat obtained from Teramana goats, an Italian indigenous breed of the Abruzzo region. Specifically, the study included a comparison with meat samples deriving from Saanen goat kids reared in the same environment and applying the same feeding protocol. Methods: Upon reaching about 7 months of age the animals were slaughtered and samples of muscle tissue were collected to be analyzed. Specifically, meat samples were subjected to evaluations of the physical parameters, including color and the meat ability to retain water, in addition to chemical evaluations that were focused to the determination of the total lipids amount, fatty acids composition, lipid oxidation, and volatile profile. Results: The meat samples obtained from the indigenous breed showed a less intense reddish color and no significant variations for the muscle tissue tendency to retain water, both regarding fresh and cooked meat. Several differences were instead observed in the fatty acid profile. The Teramana samples were richer in saturated fatty acids (p<0.01) and interestingly showed higher concentrations of rumenic acid (p<0.05), a conjugate of linoleic acid that has been associated with important health benefits for the consumers. Another important finding for these meat samples was the marked resistance to oxidative events, as evidenced by the thiobarbituric acid reactive substances-test (p<0.05) and by the characterization of the volatile profile that highlighted a strong reduction in the relative percentage of hexanal (p<0.05), commonly associated to lipid oxidation and the development of unpleasant aromatic notes. Conclusion: The collected data, therefore appeared useful for the valorization of the food product derived from the Teramana goat, although no sensory information has been collected to define the degree of acceptability by the consumers.

• Journal of Korea Society of Industrial Information Systems
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• v.26 no.6
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• pp.1-9
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• 2021

In order to increase frequency transmission efficiency, single side band(SSB) transmission systems with the complex conjugate symmetry characteristics on a frequency domain have been studied. In addition, orthogonal block codes(space-time or space-frequency block code(SFBC)) for the diversity performance gain of transmission systems have been widely researched. In this paper, we implement a 3/4-rate SFBC SSB single-carrier(SC) frequency division multiple access(FDMA) system with 4 transmit antennas. It can be shown from the simulation results that the proposed SFBC SSB SC FDMA system using the 3/4-rate 4×4 orthogonal block code outperforms the conventional SSB SC FDMA system and the 2×2 SFBC SSB SC FDMA system with 2 transmit antennas.

• Animal Bioscience
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• v.35 no.1
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• pp.105-114
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• 2022

Objective: This work was carried out to evaluate the effect of pasture (PA) feeding on buffalo meat quality compared with buffaloes reared intensively with the use of corn silage as a forage base or alternatively with polyphite meadow hay (PH). Methods: Thirty Mediterranean bull buffaloes were distributed into three experimental diet groups: maize silage (MS), PH, and PA. The animals were slaughtered at a live weight of 250 kg, and carcass and meat quality were evaluated. After 7 days of ageing, physical and chemical parameters of longissimus thoracis muscle were determined. To evaluate lipid oxidation the thiobarbituric acid reactive substances was tested at 7 and 14 days, and also the fatty acid profile was recorded by gas chromatography. Results: The PA group, even if it showed carcass parameters lower than those of the silage maize group, reported a good meat percentage (60.59% vs 58.46%, respectively) and lower fat percentage (p<0.001). PA-fed animals showed meat redness, and even if only on raw meat, shear force was higher than the others. Low values of conjugate linoleic acid, polyunsaturated fatty acids, and n-3 were reported in the silage maize group. Principal component analysis (PCA) clearly showed the influence of different diets on meat quality, and PCA1 and PCA2 explained 82% of the variability. Conclusion: Buffaloes reared on PA had meat with high nutritional value even if they showed poor carcass performance compared to the animals fed on MS. Buffaloes fed on polyphite hay were in an intermediate position, similar to grazing animals, according to the same nutritional determinations.

• Journal of Dairy Science and Biotechnology
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• v.40 no.4
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• pp.143-150
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• 2022

Maillard conjugates are formed by covalent bonds between proteins and polysaccharides. Understanding the functional properties of Maillard conjugates, including emulsifying and antioxidant properties, can be crucial when Maillard conjugates are used in processed foods. This study aimed to manufacture whey protein isolate (WPI)/Inulin Maillard conjugates and investigate how manufacturing variables including heating temperature and pH affect the functional properties of Maillard conjugates. The surface properties, emulsifying properties, and antioxidant properties of Maillard conjugates were assessed by varying heating temperature and pH. The grafting degree of WPI/Inulin Maillard conjugates increased with increasing pH and heating temperature, indicating enhanced conjugation efficiency. Surface hydrophobicity, emulsifying properties (including emulsifying activity index and emulsifying stability index), and ABTS radical scavenging ability of WPI/Inulin Maillard conjugates increased as pH and heating temperature were increased. In conclusion, WPI/Inulin Maillard conjugates were successfully manufactured, and pH and heating temperature were critical factors in enhancing Maillard conjugate functional properties.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.39 no.6
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• pp.533-540
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• 2021

High-resolution satellite images have high potential to acquire geospatial information over inaccessible areas such as Antarctica. Reference data are often required to increase the positional accuracy of the satellite data but the data are not available in many inland areas in Antarctica. Therefore this paper presents a multi-image RPCs (Rational Polynomial Coefficients) sensor modeling without any ground controls or reference data. Conjugate points between multi-images are extracted and used for the multi-image sensor modeling. The experiment was carried out for Kompsat-3A and showed that the significant accuracy increase was not observed but the approach has potential to suppress the maximum errors, especially the vertical errors.