• Asian-Australasian Journal of Animal Sciences
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• v.20 no.7
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• pp.1030-1038
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• 2007

Results on localization of growth hormone receptor (GHR), interaction of growth hormone (GH) with receptor in buffalo adipose tissue and identification of activated signaling molecules in the action of GH are presented. Bovine GH (bGH) was labeled with fluorescein or biotin. Fluorescein-labelled bGH was used for localization of GHRs in buffalo adipocytes. The receptors were present on the cell surface. The affinity of binding of GH to its receptor was determined by designing an experiment in which buffalo adipose tissue explants, biotinylated GH and streptavidin-peroxidase conjugate were employed. The affinity constant was calculated to be $2{\times}10^8M^{-1}$. The receptor density on adipose tissue was found to be 1 femto mole per mg of tissue. Signalling molecules generated in the action of GH were tentatively identified by employing Western blot and enhanced chemiluminescence techniques using anti-phosphotyrosine antibody. Based on molecular weights of proteins reactive to anti-phosphotyrosine antibody, three signaling molecules viz. insulin receptor substrate, Janus activated kinase (Jak) and mitogen activated protein were tentatively identified. These signaling molecules appeared in a time (incubation time of explants with growth hormone) dependent way. The activation of Jak2 was confirmed by employing anti-Jak2 antibody in a Western blot. The activation of Jak2 occurred during 5 min incubation of buffalo adipose tissue explants with GH and incubation for an additional period, viz. 30 min. or 60 min., resulted in a drastic reduction in activation. The results suggest that Jak2 activation is an early event in the action of GH in buffalo adipose tissue.

• Journal of Microbiology and Biotechnology
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• v.17 no.10
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• pp.1629-1637
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• 2007

An immunochromatography (ICG) strip test using a nanocolloidal gold-antibody probe was developed and optimized for the rapid detection of aflatoxin B1 (AFB1). A monoclonal antibody specific to AFB1 was produced from the cloned hybridoma cell (AF78), coupled with nanocolloidal gold, and distributed on the conjugate pad of the ICG strip test. The visual detection limit of the ICG strip test was 0.5 ng/ml, and this method showed a cross-reaction to aflatoxin B2, G1, and G2. In total, 172 grain and feed samples were collected and analyzed by both the ICG strip test and HPLC. The results of the ICG strip test showed a good agreement with those obtained by HPLC. These results indicated that the ICG strip test has a potential use as a rapid and cost-effective screening tool for the determination of AFB1 in real samples and could be applied to the preliminary screening of mycotoxin in food and agricultural products, generating results within 15 min without complicated steps.

• Korean Journal of Animal Reproduction
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• v.12 no.1
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• pp.51-62
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• 1988

Spleen cells of mouse immunized with hCG were fused with myeloma cell (SP 2/0 Ag 14) to produce monoclonal antibody against hCG. Several clones of hybridoma secreting monoclonal antibody were established and antibodies were characterized in terms of titer, subisotyping and sensitivity in immunoassay. Several methods, for the purification of anti¬bodies, based on gel-filtration, DEAE-ion exchange chromatography and affinity chromatography. were applied and compared each other by the result of SDS-PAGE. Two-site immunochemiluminometric assay (ICMA) involving the use of an excess concentration of a specific monoclonal antibody passively adsorbed onto the walls of plastic tubes and a chemiluminescence labelled antibody conjugate were de¬veloped for the determination of hCG as a preliminary study.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.10
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• pp.1498-1504
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• 2005

Competitive direct ELISA was developed to detect gentamicin residues. Mice immunized with gentamicin-keyhole limpet hemocyanin (KLH) conjugate developed good antiserum titers, which gradually increased with booster injections, indicating immunization was successfully processed. Monoclonal antibody against gentamicin was prepared using hybridoma cells cloned by limit dilution of fused cells. IgG was purified from ascites fluid of hybridoma cell-injected mice through ammonium sulfate precipitation and Sephadex G-25 gel filtration. After the gel filtration, fractions of high antibody titer were further purified through affinity chromatography on protein A/G column. Monoclonal antibody against gentamicin was confirmed as IgG1, which has kappa light chain. Cross-reactivities ($CR_{50}$) of gentamicin monoclonal antibody to other aminoglycosides (kanamycin, neomycin, and streptomycin) were less than 0.005%, indicating the monoclonal antibody was highly specific for gentamicin. Standard curve constructed through competitive direct ELISA showed measurement range (from 80 to 20% of B/$B_0$ ratio) of gentamicin was between 1 and 40 ng/ml, and 50% of B/$B_0$ ratio was about 4 ng/ml. The gentamicin concentration rapidly increased to 1,300 ng/ml after the intramuscular administration up to 2 h, then sharply decreased to less than 300 ng/ml after 4 h of withdrawal, during which the elimination half-life ($t_{1/2}$) of gentamicin in the rabbit plasma was estimated to be 1.8 h. Competitive direct ELISA method developed in this study using the prepared monoclonal antibody is highly sensitive for gentamicin, and could be useful for detecting gentamicin residues in plasma of live animals.

• Journal of the Korean Professional Engineers Association
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• v.27 no.6
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• pp.72-84
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• 1994

This is about an environmental study of groundwater interference by hydrodynamic dispersion between the well A and well C in Dongsam-dong, the Yeongdo Island, Pusan, Korea. The groundwater in the study wells come from the fracture zones deeply seated in welded lapilli rhyodacitic tuff of the Late Cretaceous Yucheon Group. The boring depth at the well A is 190 meters, and the optimal pumping rate of the well A is about 100 cubic meters per day therein. The fractured aquifers in impermeable welded tuff show the conjugate fracturing type and are of anisotropic. The aquifers along two fracture zones in the well A are 80 and 100 meters in depth, respectively. It is not suggested that those fractured aquifers are simply connected between the well A and C. The sea level fluctuation by ebb and high tides in a day is not effective to the groundwater table in the well A. The pumping for 15 days at the well A doesn't give rise to any changes of the groundwater levels in the neighbor well C. The radius of influence of the well A is measured as less than 200 meters. The measuring electric conductivity for the test of salt tracer doesn't testify any relationship between the well A and the well C. There is the main difference between the well A and the well C on the basis of the water analysis of those wells. the well A is located in the high content zone of salt much over the standard value for drinking, whereas the other wells B. C. D are in the low content area of salt below the standard value. It is elucidated for the high content zone of salt in Yeongdo around the well A to have been uplifted over 20 meters.

• Bulletin of the Korean Mathematical Society
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• v.50 no.4
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• pp.1109-1126
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• 2013

Let $\mathbb{M}^3_q(c)$ denote the 3-dimensional space form of index $q=0,1$, and constant curvature $c{\neq}0$. A curve ${\alpha}$ immersed in $\mathbb{M}^3_q(c)$ is said to be a Bertrand curve if there exists another curve ${\beta}$ and a one-to-one correspondence between ${\alpha}$ and ${\beta}$ such that both curves have common principal normal geodesics at corresponding points. We obtain characterizations for both the cases of non-null curves and null curves. For non-null curves our theorem formally agrees with the classical one: non-null Bertrand curves in $\mathbb{M}^3_q(c)$ correspond with curves for which there exist two constants ${\lambda}{\neq}0$ and ${\mu}$ such that ${\lambda}{\kappa}+{\mu}{\tau}=1$, where ${\kappa}$ and ${\tau}$ stand for the curvature and torsion of the curve. As a consequence, non-null helices in $\mathbb{M}^3_q(c)$ are the only twisted curves in $\mathbb{M}^3_q(c)$ having infinite non-null Bertrand conjugate curves. In the case of null curves in the 3-dimensional Lorentzian space forms, we show that a null curve is a Bertrand curve if and only if it has non-zero constant second Frenet curvature. In the particular case where null curves are parametrized by the pseudo-arc length parameter, null helices are the only null Bertrand curves.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.8 no.5
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• pp.1134-1140
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• 2004

In this paper, we investigated the effect of cross phase modulation(XPM) on compensation for WDM channel distortion due to chromatic dispersion, self phase modulation and XPM as a function of fiber dispersion coefficient and modulation format in 320 Gbps WDM systems. The considered WDM transmission system is based on mid-span spectral inversion(MSSI) compensation method, which has highly nonlinear dispersion shifted fiber(HNL-DSF) as nonlinear medium of optical phase conjugator (OPC) in the mid-way of total transmission line. We confirmed that the maximum channel input power resulting 1 dB eye opening penalty is reduced due to XPM effect on channel distortion, even if MSSI method was applied to WDM system. But, we confirmed that the effect of XPM on channel distortion becomes decrease as fiber dispersion coefficient of WDM system becomes larger. Futhermore, we confirmed that NRZ is better than RZ as a modulation format for similarly compensating overall WDM channels in WDM system with large fiber dispersion coefficient in order to minimize the effect of the XPM on channel distortion.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.20 no.12
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• pp.2395-2400
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• 2016

Conventional drug carriers such as liposomes, nanoparticles, polymer micelles, polymeric conjugate and lipid microemulsion for cancer chemotherapy shield normal tissues from toxic drugs to treat cancer cells in tumors. However, inaccurate tumor targeting uncontrolled drug release from the carriers and unwanted accumulation in healthy sites can limit treatment efficacy with current conventional drug carriers with insufficient concentrations of drugs in the tumors and unexpected side effects as a result. Sickle red blood cells show natural tumor preferential accumulation without any manipulation due to the adhesive interaction between molecular receptors on the membrane surface and counter-receptor on endothelial cells. In addition, structural changes of microvascular in tumor sites enhances polymerization of sickle red blood cells. In this research, we examined the use of sickle red blood cells as a new drug carrier with novel tumor targeting and controlled release properties to quantify its therapeutic effects.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.33 no.11A
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• pp.1124-1131
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• 2008

In this paper, we propose a timing synchronization scheme and its hardware architecture of the next generation IEEE 802.11n wireless LAN standard which is based on MIMO-OFDM technique. Proposed timing synchronization method takes two steps which consist of two modified auto-correlators. For coarse timing synchronization, a sliding window differentiator is used after a conventional auto-correlation in order to avoid plateau problem. The conjugate symmetry property of L-LTS is utilized for the simplification of fine timing synchronization. Since cross-correlation based methods are not required, the computational complexity and the number of multipliers can be reduced. In order to reduce the hardware complexity, we have used sign multipliers. Based on simulation results, the proposed method outperforms a conventional method. The proposed scheme can be applied to IEEE 802.11n systems and can easily be expanded to frequency synchronization scheme.

• Korean Journal of Veterinary Research
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• v.43 no.4
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• pp.677-681
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• 2003

Currently, both the indirect fluorescent antibody test (IFAT) and enzyme linked immunosorbent assay (ELISA) have been used to detect Neospora caninum antibodies. Several factors such as the buffers, the conjugate, the pattern of fluorescence, and the cross reactivity with other apicornplexan protozoan, may result in poorly correlated data. The present study was undertaken to develop and evaluate the Neospora agglutination test (NAT) for the detection and quantification of IgG antibodies to N. caninum from various animal species. Compared to the ELISA method, the NAT with a cutoff value of 1:512 gave a high index of coincidence (kappa=0.807) and no cross reactivity to Toxoplasma gondii antiserum. Hence, this NAT method, which did not require a species-specific secondary antibody and expensive tools, would be easily available for the detection of antibodies to N. caninllm of various animal species.