• Journal of Microbiology and Biotechnology
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• v.15 no.1
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• pp.221-225
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• 2005

A Staphylococcus sp. EY-3 with the capability of decolorizing Congo Red was isolated from soil at an effluent treatment plant of a textile and dyeing industry. This strain was able to almost completely decolorize a high concentration of Congo Red in 48 h under aerobic conditions. Optimal color removal (more than 96%) was achieved at 30- 40oC, and no noticeable effects of different pH values (5.5- 8.0) on decolorization were observed. This strain also exhibited a remarkable decolorization capability against azo dyes under aerobic conditions, even at a high concentration (dyes 1 g/l) of dye. The metabolic product of Congo Red degradation by this strain was identified by gas chromatography with mass selective detection (GC/MSD) to be an amine derivative benzidine.

• Textile Coloration and Finishing
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• v.2 no.1
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• pp.8-13
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• 1990

The fading of dyed material by light has long been subject of investigation, yet surprisingly little is known of the fundamental photochemical reactions, because of mainly the complex nature of dye-fibre system. The effect of the chemical structure of dye on lightfastness has been mostly studied when there is substituents on the place satisfied Hammett rule. Therefore, in this investigation the effectiveness of chemical structures of Congo Red and Benzopurpurine 4B unsatisfied Hammett rule on dyeing property and lightfastness of cotton was studied. The results obtained from this study were as follows; 1. Highly polar solvents showed hypsochromic shift. 2. Adsorption isotherm curves of the two dyes were Freundlich type. And Congo Red showed good dyeing property in comparision with Benzopurpurine 4B. 3. The type of the FR curve of the two dyes was first-ordr curve. And the CF curves of the two dyes showed normal fading state during irradiation. 4. During irradiation, the Hue was changed on the part of yellowish. The Value was increasing and the Chroma was decreasing. 5. Benzopurpurine 4B showed good lightfastness in comparision with Congo Red.

• The Korean Journal of Mycology
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• v.37 no.1
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• pp.73-79
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• 2009

As waste-water disposal plants and oxidative biodegradation for the removal of waste polyaromatic dyes are proved to be ineffective due to the chemical stability of dyes, we studied various strains of mushroom fungi for the removal of these dyes. 100 fungi were isolated from the mushroom samples of 230 species collected in Korea. The growth medium containing a dye (Bromophenol Blue, Congo Red, or Methylene Blue) was inoculated to 10% and incubated for 7 days without shaking. The six strains which removed dyes effectively were selected for further studies with respect to removal of polycyclic aromatic dyes. For all strains, the rate of decoloration of dyes was increasing with Methylene Blue, Bromophenol Blue and Congo Red. The rate of decoloration was higher with stationary culture than with shaking culture. Adsorption of the dyes was the highest with Congo Red.

• Journal of Microbiology and Biotechnology
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• v.23 no.6
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• pp.843-849
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• 2013

A dye-decolorizing bacterium was isolated from a soil sample and identified as Bacillus thuringiensis using 16S rRNA sequencing. The bacterium was able to decolorize three different textile dyes, namely, Reactive blue 13, Reactive red 58, and Reactive yellow 42, and a real dyehouse effluent up to 80-95% within 6 h. Some non-textile industrially important dyes were also decolorized to different extents. Fourier transform infrared spectroscopy and gas chromatography-mass spectrometer analysis of the ethyl acetate extract of Congo red dye and its metabolites showed that the bacterium could degrade it by the asymmetric cleavage of the azo bonds to yield sodium (4-amino-3-diazenylnaphthalene-1-sulfonate) and phenylbenzene. Sodium (4-amino-3-diazenylnaphthalene-1-sulfonate) was further oxidized by the ortho-cleavage pathway to yield 2-(1-amino-2-diazenyl-2-formylvinyl) benzoic acid. There was induction of the activities of laccase and azoreductase during the decolorization of Congo red, which suggests their probable role in the biodegradation. B. thuringiensis was found to be versatile and could be used for industrial effluent biodegradation.

• Korean Journal of Agricultural Science
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• v.13 no.1
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• pp.139-146
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• 1986

The characteristics of the amyloid proteins were histochemically investigated with the amyloidladen organs from bovine(15 cases), canine(1 case), feline(1 case), and artificially induced rabbit amyloidosis(1 case). The amyloid-laden organs from bovine, canine and experimental rabbit amyloidosis showed potassium-permanganate-senstive reaction, revealing complete disappearance for Congo red affinity, loss of brick-red colored fluorescence and green birefringence. From these findings, the amyloids from bovine, canine and experimentally induced rabbit amyloidosis were thought to be equivalent to amyloid protein A (AA protein). In the present feline case with amyloidosis, however, the amyloid proteins revealed potassium permanganate-resistant reaction, showing unchanged affinity for Congo red, and immunoglobulin was also deposited in the glomeruli of the kidney. From these findings, the amyloid proteins from feline case with amyloidosis were considered to be equivalent to amyloid light chain-related proteins (AL protein).

• Mycobiology
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• v.38 no.1
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• pp.74-77
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• 2010

To obtain basic information on the detection of cellulolytic activity in Auricularia auricula-judae, the influences of dye reagent, pH, and temperature were assessed. Chromogenic dye (congo red, phenol red, remazol brilliant blue, and trypan blue) was individually incorporated into a medium containing either carboxymethyl-cellulose, Avicel, or D-cellobiose as a polysaccharide carbon substrate. The other assessments utilized pHs ranging from 4.5 to 8.0 and temperatures from $15\sim35^{\circ}C$. Overall, when A. auricula-judae species were transferred onto media contained Congo red and adjusted pH 7.0 and then incubated at $25^{\circ}C$ for 5 days, the clear zone indicative of cellulolytic activity was more pronounced.

• Journal of Applied Biological Chemistry
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• v.44 no.1
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• pp.27-31
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• 2001

Using a new evaluation method, Congo red method, the foliar uptakes of eight fungicides were measured. Among the fungicides tested, fenarimol, myclobutanil, edifenphos, isoprothiolane, and tricyclazole were found to be penetrable (11.0~42.8%), and were continuously absorbed into the leaf regardless of their formulation types. Procymidone, dimethomorpb, and ethaboxan were seldomly absorbed into plant leaf. The uptakes of dimethomorph and ethaboxam were facilitated (18.1~22.7%) in the presence of Koremul-OE-20, as an activator, showing plateau phase at 12h after application. Foliar uptake of fenarimol and myclobutanil into cucumber plant by spray application was larger than that by dropping application, while observed opposite in the cases of dimethomorph and ethaboxam containing activator. Congo red method in conjunction with spraying application used in the present study produced the clear results on foliar uptake of fungicides and therefore proved to be better than the conventional method in which the dropping application of pesticide has been used.

• Textile Coloration and Finishing
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• v.3 no.3
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• pp.15-22
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• 1991

The effects of electrolytes on color fastness properties of cotton fabric with Congo Red have been studied at $90^{\circ}C$. Each dyeing carried with $10 \times 10^{-6}M$ of Congo Red and with various concentration of electrolytes. The results obtained from this study were as follow 1. In the case of none, the fastness to washing became worse, but the fastness to light better with increasing dye concentration. 2. In the case of additing an electrolyte, the fastness to washing became worse and the fastness to light better than the case of none. The samples had become yellower after washing test. Yellowness had been reduced after fade-O-meter test. 3. What kind of electrolytes could not have any effect on the fastness to washing, but they influenced the fastness to light in the order of LiCl

• Microbiology and Biotechnology Letters
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• v.12 no.4
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• pp.305-309
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• 1984

A selective medium which allows growth of only cellulolytic bacteria was developed. The medium composed of 0.5% carboxymethylcellulose (CMC), 0.005% yeast extract, minerals and agar. Colony formation on this medium indicates overall activities of cellulose utilization. A subsequent test with Congo Red dye could distinguish extracellular cellulolysis from intracellular type.

• Analytical Science and Technology
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• v.14 no.3
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• pp.259-265
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• 2001

Photocatalytic degradation of Congo red was performed using various semiconductors as ZnO, CdS, rutile-$TiO_2$ or mixed rutile-$TiO_2$/ZnO. The change of degradation of the dye was investigated by UV-visible spectrophotometric method. The photocatalytic action of CdS was greater than ZnO and rutile-$TiO_2$ in account of low band gap energy of CdS. The rate of photocatalytic degradation reaction increased drastically in according to increasing ratio of ZnO on mixed rutile-$TiO_2$/ZnO. These photocatalytic effect of rutile-$TiO_2$ was suppressed by more stable rutile-$TiO_2$, doping the hydrolysis product with $Zn^{2+}$ prior to calcination onto the rutile-$TiO_2$ surface.