DOI QR코드

DOI QR Code

버섯균에 의한 염료의 탈색

Decoloration of Polycyclic Aromatic Dyes by Mushroom Fungi

  • ;
  • ;
  • 서승염 (공주대학교 자연과학대학)
  • 발행 : 2009.06.30

초록

화학적으로 안정한 다환 방향족 염료을 페수처리시설이나 천연적인 분해에 통해서 제거시키는 것이 비효율적인 것으로 알려져 있다. 다환 방향족 염료를 제거를 위하여 우리는 여러 버섯균류의 조사하였다. 한국의 야산에서 채집된 230종의 버섯으로부터 조직분리를 통하여 100개 버섯 균을 분리하였다. 염료(Bromophenol Blue, Congo Red, 혹은 Methylene Blue)를 함유한 배지에 분리된 버섯 균을 10%로 접종한 후 7일 동안 정치배양 혹은 진탕배양하여 염료제거능이 우수한 6 종의 버섯 균을 선발하였다. 이렇게 선발된 버섯 균을 대상으로 다핵방향족 염료를 제거하는 능력을 조사하였다. 모든 버섯 균이 진탕배양 시보다 정치배양 시 더 우수한 염료제거 능을 보였다. 염료에 따른 염료제거능은 대부분의 경우에서 Methylene Blue, Bromophenol Blue, Congo Red 순으로 증가하였다. 위 세 염료 중에서 Congo Red가 가장 많이 흡착되었다.

As waste-water disposal plants and oxidative biodegradation for the removal of waste polyaromatic dyes are proved to be ineffective due to the chemical stability of dyes, we studied various strains of mushroom fungi for the removal of these dyes. 100 fungi were isolated from the mushroom samples of 230 species collected in Korea. The growth medium containing a dye (Bromophenol Blue, Congo Red, or Methylene Blue) was inoculated to 10% and incubated for 7 days without shaking. The six strains which removed dyes effectively were selected for further studies with respect to removal of polycyclic aromatic dyes. For all strains, the rate of decoloration of dyes was increasing with Methylene Blue, Bromophenol Blue and Congo Red. The rate of decoloration was higher with stationary culture than with shaking culture. Adsorption of the dyes was the highest with Congo Red.

키워드

참고문헌

  1. Abadulla, E., Tzanov, T., Costa, S., Robra, K. H., Cavaco-Paulo, A. and Gubitz, G. M. 2000. Decoloration and detoxification of textile dyes with a laccase from Trametes hirsuta. Appl. Environ. Microbiol. 66: 3357-3362. https://doi.org/10.1128/AEM.66.8.3357-3362.2000
  2. An, H., Qian, Y., Gu, X. and Tang, W. Z. 1996. Biological treatment of dye wastewaters using an anaerobic-oxic system. Chemosphere 33: 2533-2542. https://doi.org/10.1016/S0045-6535(96)00349-9
  3. Cripps, C., Bumpus, J. A. and Aust, S. D. 1990. Biodegradation of azo and heterocyclic dyes by Phanerochaete chrysosporium. Appl. Environ. Microbiol. 56: 1114-1118.
  4. Delee, W., O'Neill, C., Hawkes, F. R. and Pinheiro, H. M. 1998. Anaerobic treatment of textile effluents: a review. J. Chem. Technol. Biotechnol. 73: 323-335. https://doi.org/10.1002/(SICI)1097-4660(199812)73:4<323::AID-JCTB976>3.0.CO;2-S
  5. Desphande, S. D. 2001. Ecofriendly dyeing of synthetic fibres. Ind. J. Fibre Text. Res. 26: 136-142.
  6. Forgacs, E., Cserha'ti, T., and Oros, G. 2004. Removal of synthetic dyes from wastewaters: a review. Environment International 30: 953-971. https://doi.org/10.1016/j.envint.2004.02.001
  7. Gupta, G. S., Shukla, S. P., Prasad. G. and Singh, V. N. 1992. China clay as an adsorbent for dye house wastewaters. Environ. Technol. 13: 925-936. https://doi.org/10.1080/09593339209385228
  8. Hao, O. J., Kim, H. and Chiang, P. C. 2000. Decolorization of wastewater. Crit. Rev. Environ. Sci. Technol. 30: 449-502. https://doi.org/10.1080/10643380091184237
  9. Hsu, T. C and Chiang, C. S. 1997. Activated sludge treatment of dispersed dye factory wastewater. J. Environ. Sci. Health. Part A, Environ. Sci. Eng. Toxic Hazard. Substance Control A. 32: 1921-1932. https://doi.org/10.1080/10934529709376655
  10. Ivanov, K., Gruber, E., Schempp, W. and Kirov, D. 1996. Possibilities of using zeolite as filler and carrier for dye stuffs in paper. Das Papier 50: 456-460 [in German].
  11. Jger, A., Croan, S. and Kirk, T. K. 1985. Production of ligninases and degradation of lignin in agitated submerged cultures of Phanerochaete chrysosporium. Appl. Environ. Microbiol. 50: 1274-1278.
  12. Kabadasil, I., Tnay, O. and Orhon, D. 1999. Wastewater control and management in a leather tanning district. Water Sci. Technol. 40: 261-267. https://doi.org/10.1016/S0273-1223(99)00393-5
  13. Kanekar, P. and Sarnaik, S. 1991. An activated sludge process to reduce the pollution load of a dye-industry waste. Environ. Pollut. 70: 27-33. https://doi.org/10.1016/0269-7491(91)90129-K
  14. Kirk, T. K., Nakatsubo, F. and Reid, I. D. 1983. Further study discounts role for singlet oxygen in fungal degradation of lignin model compounds. Biochem Biophys Res Commun. 111: 200-204. https://doi.org/10.1016/S0006-291X(83)80136-3
  15. Kuo, W. G. 1992. Decolorizing dye wastewater with Fenton's reagent. Water Res. 26: 881-886. https://doi.org/10.1016/0043-1354(92)90192-7
  16. Leatham, G. F., Crawford, R. L. and Kirk, T, K. 1983. Degradation of Phenolic Compounds and Ring Cleavage of Catechol by Phanerochaete chrysosporium. Appl. Environ. Microbiol. 46: 191-197.
  17. Leisola, M. S., Kozulic, B., Meussdoerffer, F. and Fiechter, A 1987. Homology among multiple extracellular peroxidases from Phanerochaete chrysosporium. J. Biol. Chem. 262: 419-424.
  18. Maurya, N. S., Mittal, A. K, Cornel, P. and Rother, E. 2006. Biosorption of dyes using dead macro fungi: effect of dye structure, ionic strength and pH. Bioresour Technol. 97: 512-521. https://doi.org/10.1016/j.biortech.2005.02.045
  19. Morgan-Sagastume, J. M, Jimenez, B. and Noyola, A. 1997. Tracer studies in a laboratory and pilot scale UASB reactor. Environ. Technol. 18: 817-826. https://doi.org/10.1080/09593331808616600
  20. Namasivayam, C. and Yamuna, R. T. 1992. Removal of rhodamine-B by biogas waste slurry from aqueous solution. Water Air Soil Pollut. 65:1 01-109. https://doi.org/10.1007/BF00482752
  21. Ollikka, P., Alhonmki, K., Leppnen, V. M., Glumoff, T., Raijola, T. and Suominen, I. 1993. Decolorization of Azo, Triphenyl Methane, Heterocyclic, and Polymeric Dyes by Lignin Peroxidase Isoenzymes from Phanerochaete chrysosporium. Appl. Environ. Microbiol. 59: 4010-4016.
  22. Orth, A. B., Royse, D. J. and Tien, M. 1993. Ubiquity of lignin-degrading peroxidases among various wood-degrading fungi. Appl. Environ. Microbiol. 59: 4017-23.
  23. Pacioni. 1981. Simon and Schuster's Guide to Mushrooms. Simon and Schuster Inc. New York, USA.
  24. Podgronik, H., Podgronik, A., Milavec, P. and Perdih, A 2001. The effect of agitation and nitrogen concentration on lignin peroxidase (LiP) isoform composition during fermentation of Phanerochaete chrysosporium. J. Biotechnol. 88: 173-176. https://doi.org/10.1016/S0168-1656(01)00270-X
  25. Reutergradh, L. B. and Iangpashuk, M. 1997. Photocatalytic decolorization of reactive azo dye: a comparison between TiO2 and CdS photocatalysis. Chemosphere 35: 585-596. https://doi.org/10.1016/S0045-6535(97)00122-7
  26. Roy, D., Wang, G. T. and Adrian, D. D. 1993. A simplified solution technique for carbon adsorption model. Water Res. 27: 1033-1040. https://doi.org/10.1016/0043-1354(93)90067-R
  27. Scarpi, C., Ninci, F., Centini, M. and Anselmi, C. 1998. Highperformance liquid chromatography determination of direct and temporary dyes in natural hair colourings. J. Chromatogr A. 796: 319-325. https://doi.org/10.1016/S0021-9673(97)01015-7
  28. Schliephaje, K. and Lonergan, G. T. 1996. Laccase variation during dye decolorisation in a 200 L packed-bad bioreactor. Biotechnol. Lett. 18: 881-886. https://doi.org/10.1007/BF00154614
  29. Shaul, G. M., Holdsworth, T. J., Dempsey, C. R. and Dostal, K. A. 1991. Fate of water soluble azo dyes in the activated sludge process. Chemosphere 22: 107-119. https://doi.org/10.1016/0045-6535(91)90269-J
  30. Shimada, M., Nakatsubo, F., Higuchi, T. and Kirk, T. K. 1981. Biosynthesis of the secondary metabolite veratryl alcohol in relation to lignin degradation in Phanerochaete chrysosporium. Arch. Microbiol. 129: 321-324. https://doi.org/10.1007/BF00414706
  31. Stolz, A. 2001. Basic and applied aspects in the microbial degradation of azo dyes. Appl. Microbiol. Biotechnol. 56: 69-80. https://doi.org/10.1007/s002530100686
  32. Tang, W. Z. and An, H. 1995. UV/TiO2 photocatalytic oxidation of commercial dyes in aqueous solutions. Chemosphere 31:4158-4170. https://doi.org/10.1016/0045-6535(95)80015-D
  33. Venkatadri, R. and Irvine, R. L. 1990. Effect of agitation on ligninase activity and ligninase production by Phanerochaete chrysosporium. Appl. Environ. Microbiol. 56: 2684-2691.
  34. Wagner, R. W. and Lindsey, J. S. 1996. Boron-dipyrromethane dyes for incorporation in synthetic multi-pigment lightharvesting arrays. Pure Appl. Chem. 68: 1373-1380. https://doi.org/10.1351/pac199668071373
  35. Wang, X. Y. and Yu, J. 1998. Adsorption and degradation of synthetic dyes on the mycelium of Trametese versicolor. Water Sci. Technol. 38: 233238. https://doi.org/10.1016/S0273-1223(98)00532-0
  36. Windholz, M., Budavari S., Blumettiet R. F., and Otterbein. E.S. 1983, The Merck Index. Rahway N. J., U. S. A.
  37. Wolff, S. P., Garner, A. and Dean, R. T. 1986. Free radicals, lipids and protein degradation. Trends Biochem. Sci. 11: 27-31 https://doi.org/10.1016/0968-0004(86)90228-8
  38. Young, L. and Yu, J. 1997. Ligninase-catalysed decolorization of synthetic dyes. Water Res. 31: 1187-1193. https://doi.org/10.1016/S0043-1354(96)00380-6
  39. Zhang, T. C., Fu, Y. C. and Bishop, P. L. 1995. Transport and biodegradation of toxic organics in biofilms. J. Hazard Mater 41: 267-285. https://doi.org/10.1016/0304-3894(94)00118-Z