• Title/Summary/Keyword: Concentration measurement

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Measurement of Bacterial (Escherichia coil) Concentration by Flow Cytometry

  • Ji, Suk;Lee, Jung-Ok;Choi, Young-Nim
    • International Journal of Oral Biology
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    • v.30 no.2
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    • pp.65-69
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    • 2005
  • Periodontitis is a multi-microbial disease and the comparison of a series of periodontopathogenic and non-periodontopathogenic bacteria in terms of microbe-host interaction may provide clues to understand the microbial etiology of the disease better. When we deal with twenty different bacterial species in a study, the first technical issue is how to measure the accurate concentration and use the same number of bacterial cells. We measured bacterial concentration by enumerating bacteria stained with SYTOX green for constant time using a flow cytometer and compared the results with those obtained by plate counting. Concentrations calculated by two different methods were very close. Therefore, flow cytometric counting allowed the rapid analysis of live/dead bacteria, offering the advantage of turbidity measurement and that of colony counting together.

Wear Debris Coacentration Measurement by Laser Beam Attenuation (광감쇄를 이용한 마모입자의 농도 측정)

  • 강기호;손정영;전형욱;윤의성;안효석
    • Tribology and Lubricants
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    • v.6 no.2
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    • pp.94-98
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    • 1990
  • The size and concentration of wear debris in lubricating oil often reveal the operating condition of the rotating machinery. To evaluate the possible application of light attenuation measurement for the estimation of wear debris concentration in the lubricating oil, the light transmittance through the lubricating oil cell contaminated with various concentrations of diatomire particles was measured, the attenuation coefficient was estimated from the transmittance measurement and the coefficients were compared with those obtained from the scattering theory. The comparision showed good agreements between them. It is also noted that the experimentally determined attenuation coefficient showed almost linear relation with particle weight coucentrations for the concentrations within the range of 2000 ppm. For the case of 0 ppm weight concentration of diatomire particles in the lubricating oil cell, the thickness of the cell required to give $100 \muW$ light attenuation is 7.75 mm. This result indicates that the light attenuation method will be one of the possible candidates of machine failure diagnostic sensors for the estimation of wear debris concentration in the lubricating oil.

Can $CO_2$ concentration at one level of eddy covariance measurement be used to estimate storage term over forest\ulcorner

  • Choi, Tae-Jin;Chae, Nam-Yi;Kim, Joon;Lim, Jong-Hwan
    • Proceedings of the Korean Environmental Sciences Society Conference
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    • 2003.11a
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    • pp.47-50
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    • 2003
  • $CO_2$ concentration profile was measured to investigate whether $CO_2$ concentration at one level (i.e., eddy covariance measurement level) can be used to estimate storage term without significant uncertainty at broadleaf deciduous forest at Kwangneung experiment forest in Korea. Based on t-test with significance level of 5%, there was no statistical difference between storage term from one-level $CO_2$ concentration and one from $CO_2$ profile measurement. Storage term constitutes on average 5% of half hourly net ecosystem exchange (NEE) even at unstable stability (i.e., well mixed condition), indicating that storage term should be considered even at daytime, which is sometimes neglected.

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A Study on the Turbulent Characteristics of Rushton Turbine Mixer by Simultaneous Measurement of Velocity and Concentration Field with Stereo-PIV/PLIF Technique (Stereo-PIV/PLIF의 속도장과 농도장 동시측정 기법을 이용한 러쉬톤 교반기내 난류특성에 관한 연구)

  • Min, Young-Uk;Kim, Yun-Gi;Kim, Kyung-Chun
    • Proceedings of the KSME Conference
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    • 2003.11a
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    • pp.694-699
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    • 2003
  • Simultaneous measurement with PLIF(Planar Laser-Induced Fluorescence) and Stereo-PIV(Stereoscopic Particle Image Velocimetry) was performed to investigate the structural characteristics of flow field in Rushton Turbine Mixer. Instantaneous 3D velocity fields are measured by two 2K ${\times}$ 2K CCD cameras focused on an object plane with the angular displacement methods while the concentration fields are obtained through the measurement of the fluorescence intensity of Rhodamine B tracer excited by the second pulse of Nd:Yag laser light. Image distortion due to the camera view-angle is compensated by a mapping function. Finally, the spatial structures of turbulent mixing around Rushton turbine were identified by the calculation of cross-correlation fields between the velocity and concentration field.

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Development of a Concentration Measurement System for Pickling Line Control (산세라인 자동화를 위한 농도 측정 시스템 개발)

  • Park, Hyoung-Kuk;Lee, Jong-Hyun;Noh, Ill-Hwan
    • Journal of Institute of Control, Robotics and Systems
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    • v.19 no.10
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    • pp.891-895
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    • 2013
  • This paper proposes the development of a new method for online analysis which measured acid concentration in a pickling line. Pickling is the most important step to remove surface scale layers and is strongly depending on the exactly controlled pickling liquor composition. Today, there is no feasible system available for the online control of pickling lines. Within this paper, new methods for online analysis of pickling liquors have been tested and implemented into an overall pickling process control tool. This method measured simultaneously the hydrochloric acid and iron ion concentration in a solution of hydrochloric acid by measuring the ultrasonic speed, the solution temperature, and the electrical conductivity. Experimental results showed excellent precision and the measurement error was ${\pm}2g/l$ compared with the neutralization titration measurement.

Development of an Efficiency Calibration Model Optimization Method for Improving In-Situ Gamma-Ray Measurement for Non-Standard NORM Residues (비정형 공정부산물 In-Situ 감마선 측정 정확도 향상을 위한 효율교정 모델 최적화 방법 개발)

  • WooCheol Choi;Tae-Hoon Jeon;Jung-Ho Song;KwangPyo Kim
    • Journal of Radiation Industry
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    • v.17 no.4
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    • pp.471-479
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    • 2023
  • In In-situ radioactivity measurement techniques, efficiency calibration models use predefined models to simulate a sample's geometry and radioactivity distribution. However, simplified efficiency calibration models lead to uncertainties in the efficiency curves, which in turn affect the radioactivity concentration results. This study aims to develop an efficiency calibration optimization methodology to improve the accuracy of in-situ gamma radiation measurements for byproducts from industrial facilities. To accomplish the objective, a drive mechanism for rotational measurement of an byproduct simulator and a sample was constructed. Using ISOCS, an efficiency calibration model of the designed object was generated. Then, the sensitivity analysis of the efficiency calibration model was performed, and the efficiency curve of the efficiency calibration model was optimized using the sensitivity analysis results. Finally, the radiation concentration of the simulated subject was estimated, compared, and evaluated with the designed certification value. For the sensitivity assessment of the influencing factors of the efficiency calibration model, the ISOCS Uncertainty Estimator was used for the horizontal and vertical size and density of the measured object. The standard deviation of the measurement efficiency as a function of the longitudinal size and density of the efficiency calibration model decreased with increasing energy region. When using the optimized efficiency calibration model, the measurement efficiency using IUE was improved compared to the measurement efficiency using ISOCS at the energy of 228Ac (911 keV) for the nuclide under analysis. Using the ISOCS efficiency calibration method, the difference between the measured radiation concentration and the design value for each simulated subject measurement direction was 4.1% (1% to 10%) on average. The difference between the estimated radioactivity concentration and the design value was 3.6% (1~8%) on average when using the ISOCS IUE efficiency calibration method, which was closer to the design value than the efficiency calibration method using ISOCS. In other words, the estimated radioactivity concentration using the optimized efficiency curve was similar to the designed radioactivity concentration. The results of this study can be utilized as the main basis for the development of regulatory technologies for the treatment and disposal of waste generated during the operation, maintenance, and facility replacement of domestic byproduct generation facilities.

Uncertainty evaluation in electrochemical noise resistance measurement (전기화학적 노이즈 저항 측정에서의 불확도 평가)

  • Kim, Jong Jip;Kang, Su Yeon
    • Corrosion Science and Technology
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    • v.12 no.5
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    • pp.220-226
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    • 2013
  • The uncertainty in statistical noise resistance measurement was evaluated for a type 316 stainless steel in NaCl solutions at room temperature. Sensitivity coefficients were determined for measurands or variables such as NaCl concentration, pH, solution temperature, surface roughness, inert gas flow rate and bias potential amplitude. The coefficients were larger for the variables such as NaCl concentration, pH, inert gas flow rate and solution temperature, and they were the major factors increasing the combined standard uncertainty of noise resistance. However, the contribution to the uncertainty in noise resistance measurement from the above variables was remarkably low compared to that from repeated measurements of noise resistance, and thus, it is difficult to lower the uncertainty in noise resistance measurement significantly by lowering the uncertainties related with NaCl concentration, pH, inert gas flow rate and solution temperature. In addition, the uncertainty in noise resistance measurement was high amounting to 17.3 % of the mean, indicating that the reliability in measurement of noise resistance is low.

Setting the Current Air Quality Concentration Using the National Atmosphere Measurement Network

  • CHO, Dong-Myung;LEE, Ju-Yeon;KWON, Lee-Seung;KIM, Su-Hye;KWON, Woo-Taeg
    • Journal of Wellbeing Management and Applied Psychology
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    • v.4 no.3
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    • pp.23-33
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    • 2021
  • Purpose: In the course of the domestic environmental impact assessment, the current status survey was improperly conducted, and the issue of false and inaccurate environmental impact assessment reports has been raised several times recently through media reports. Research design, data and methodology: There is a continuous demand for improvement measures for the current status measurement method, such as having difficulties in securing a normal measurement date in consideration of equipment operation and rainfall days in the field. Results: In addition, in order to grasp the general air quality status of the evaluation target area, it is necessary to check the various current status concentrations by season and time series per year. However, there is a problem that is currently being carried out based on limited results such as measurement for 1 day or 3 days. Conclusions: Therefore, in this study, based on the national atmospheric measurement network, an inverse distance weighted (IDW) interpolation method was applied to calculate the current state concentration. This study suggested a method to use it in preparing the air quality item in the environmental impact assessment report.

Simultaneous Measurement of Liquid-level, Concentration and Temperature of a Urea Tank using Ultrasonic and Electrical Conductivity Sensors (초음파와 전기전도 센서를 이용한 우레아 탱크 수위, 농도 및 온도의 동시 측정 방안)

  • Choi, Byungchul;Kim, Taewook
    • Journal of Power System Engineering
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    • v.21 no.5
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    • pp.71-78
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    • 2017
  • The purpose of this study is to propose the basic data for the development of a sensor capable of simultaneously measuring the liquid-level, concentration and temperature of a urea tank using ultrasonic and electrical conductivity sensors for diesel vehicles with a urea-SCR system. It was found that the liquid-level of the urea tank using the ultrasonic sensor showed a good linearity with the actual liquid-level, and the urea concentration maintained good linearity in the range of 32.5 wt% to 10 wt%. It was an effective measurement of urea concentration to use the electrical conductivity sensor in the temperature range of $-10{\sim}22^{\circ}C$ and to use the ultrasonic sensor at $22^{\circ}C$ or more. Simultaneous measurement of concentration, liquid-level and temperature of the urea tank will be possible by attaching the electrical conductivity sensor and the ultrasonic sensor (split-type) to one sensor together.

On-line Measurement of $H_2$O/LiBr Concentration using Electric Conductivity (전기 전도도를 이용한 $H_2$O/LiBr 용액의 실시간 농도의 측정)

  • 박찬우;김정환;강용태
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.14 no.12
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    • pp.1078-1083
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    • 2002
  • The objectives of this paper are to measure the concentration of $H_2$O/LiBr solution by measuring the electrical conductivity and to study the effect of the solution temperature and the concentration on the electrical conductivity of the solution. The solution temperature ranges $20^{\circ},\;40^{\circ},\;and\; 60^{\circ}$ for a fixed concentration during the experiment. The valid ranges of the concentration are two regions, low concentration region (1~20% of LiBr) and high concentration region (55~66% of LiBr). The results show that the conductivity of the solution increases linearly with increasing the solution temperature while it increases without creasing the concentration lower than about 35% of LiBr and decreases with increasing the concentration higher than 35%. This paper proposes experimental correlations for the concentration as functions of the solution temperature and the concentration with error band of $\pm7$% for the low concentration region and $\pm1$% for the high concentration region, respectively. The experimental correlation can be practically used in the on-line measurement without any sampling of solution from the closed system.