• 한국식품과학회지
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• 제34권1호
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• pp.65-72
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• 2002

식혜 제조시 당화에 영향을 미치는 인자인 엿기름 추출온도와 시간, 엿기름과 물의 비율, 밥의 양을 반응표면분석법의 중심합성계획에 따라 설계하여 각각 조건들의 영향을 dual cathod system으로 구성된 바이오센서로 행하였을 때 포도당과 맥아당의 측정은 엿기름 추출온도 및 각각의 조건들은 포도당과 맥아당의 최적조건이 약간은 다른 결과를 보였는데 포도당에서는 $60^{\circ}C$, 맥아당은 $55^{\circ}C$에서 최적의 온도를 나타내었고 엿기름 추출시간은 포도당이 6시간 30분, 맥아당은 5시간 45분이었으며 물과 엿기름의 비율은 포도당과 맥아당 각각 1:6.3, 1:8.8이었고 엿기름 추출액과 고두밥의 양에서는 0.48%는 포도당, 0.6%는 맥아당이 최적조건이었다. 반응표면분석법에 의하여 유의차를 검증한 결과 그 상관관계를 비교했을 때 유의성이 높게 나타났다.

• The Journal of Advanced Prosthodontics
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• 제12권4호
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• pp.233-238
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• 2020

PURPOSE. This study aims to compare the marginal fitness of two types of implant-supported fixed dental prosthesis, i.e., cementless fixation (CL.F) system and cement-retained type. MATERIALS AND METHODS. In each group, ten specimens were assessed. Each specimen comprised implant lab analog, titanium abutment fabricated with a 2-degree tapered axial wall, and zirconia crown. The crown of the CL.F system was retained by frictional force between abutment and relined composite resin. In the cement-retained type, zinc oxide eugenol cement was used to set crown and abutment. All specimens were sterilized with ethylene oxide, immersed in Prevotella intermedia culture in a 50 mL tube, and incubated with rotation. After 48 h, the specimens were washed thoroughly before separating the crown and abutment. The bacteria that penetrated into the crown-abutment interface were collected by washing with 500 µL of sterile saline. The bacterial cell number was quantified using the agar plate count technique. The BacTiter-Glo Microbial Cell Viability Assay Kit was used to measure bacterial adenosine triphosphate (ATP)-bioluminescence, which reflects the bacterial viability. The t-test was performed, and the significance level was set at 5%. RESULTS. The number of penetrating bacterial cells assessed by colony-forming units was approximately 33% lower in the CL.F system than in the cement-retained type (P<.05). ATP-bioluminescence was approximately 41% lower in the CL.F system than in the cement-retained type (P<.05). CONCLUSION. The CL.F system is more resistant to bacterial penetration into the abutment-crown interface than the cement-retained type, thereby indicating a precise marginal fit.

• Journal of Microbiology and Biotechnology
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• 제33권4호
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• pp.419-429
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• 2023

Ultraviolet C (UV-C, 200-280 nm) light has germicidal properties that inactivate a wide range of pathogenic and spoilage microorganisms. UV-C has been extensively studied as an alternative to thermal decontamination of fruit juices. Recent studies suggest that the efficacy of UV-C irradiation in reducing microorganisms in fruit juices is greatly dependent on the characteristics of the target microorganisms, juice matrices, and parameters of the UV-C treatment procedure, such as equipment and processing. Based on evidence from recent studies, this review describes how the characteristics of target microorganisms (e.g., type of microorganism/strain, acid adaptation, physiological states, single/composite inoculum, spore, etc.) and fruit juice matrices (e.g., UV absorbance, UV transmittance, turbidity, soluble solid content, pH, color, etc.) affect the efficacy of UV-C. We also discuss the influences on UV-C treatment efficacy of parameters, including UV-C light source, reactor conditions (e.g., continuous/batch, size, thickness, volume, diameter, outer case, configuration/arrangement), pumping/flow system conditions (e.g., sample flow rate and pattern, sample residence time, number of cycles), homogenization conditions (e.g., continuous flow/recirculation, stirring, mixing), and cleaning capability of the reactor. The collective facts indicate the immense potential of UV-C irradiation in the fruit juice industry. Existing drawbacks need to be addressed in future studies before the technique is applicable at the industrial scale.

• Journal of Animal Science and Technology
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• 제60권11호
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• pp.27.1-27.8
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• 2018

Background: Enteric methane ($CH_4$) accounts for about 70% of total $CH_4$ emissions from the ruminant animals. Researchers are exploring ways to mitigate enteric $CH_4$ emissions from ruminants. Recently, nano zinc oxide (nZnO) has shown potential in reducing $CH_4$ and hydrogen sulfide ($H_2S$) production from the liquid manure under anaerobic storage conditions. Four different levels of nZnO and two types of feed were mixed with rumen fluid to investigate the efficacy of nZnO in mitigating gaseous production. Methods: All experiments with four replicates were conducted in batches in 250 mL glass bottles paired with the ANKOM$^{RF}$ wireless gas production monitoring system. Gas production was monitored continuously for 72 h at a constant temperature of $39{\pm}1^{\circ}C$ in a water bath. Headspace gas samples were collected using gas-tight syringes from the Tedlar bags connected to the glass bottles and analyzed for greenhouse gases ($CH_4$ and carbon dioxide-$CO_2$) and $H_2S$ concentrations. $CH_4$ and $CO_2$ gas concentrations were analyzed using an SRI-8610 Gas Chromatograph and $H_2S$ concentrations were measured using a Jerome 631X meter. At the same time, substrate (i.e. mixed rumen fluid+ NP treatment+ feed composite) samples were collected from the glass bottles at the beginning and at the end of an experiment for bacterial counts, and volatile fatty acids (VFAs) analysis. Results: Compared to the control treatment the $H_2S$ and GHGs concentration reduction after 72 h of the tested nZnO levels varied between 4.89 to 53.65%. Additionally, 0.47 to 22.21% microbial population reduction was observed from the applied nZnO treatments. Application of nZnO at a rate of $1000{\mu}g\;g^{-1}$ have exhibited the highest amount of concentration reductions for all three gases and microbial population. Conclusion: Results suggest that both 500 and $1000{\mu}g\;g^{-1}$ nZnO application levels have the potential to reduce GHG and $H_2S$ concentrations.

• 한국습지학회지
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• 제22권4호
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• pp.312-320
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• 2020

도시 환경문제 및 개발사업 환경영향 저감을 위하여 자연적 물 순환 기능을 가진 다양한 LID가 적용되고 있다. 그러나 LID 시설의 과도한 침투와 증발산은 LID 내부 토양을 건조화시켜 식물과 미생물 활동성을 떨어뜨리고 환경저감 능력을 감소시킨다. 본 연구는 LID 시설의 관리 방안을 도출하기 위하여 복합적인 센서를 적용한 실시간 측정 시스템을 개발하고자 하였다. 측정 가능한 센서와 사물인터넷(IoT) 적용 실험은 아크릴 상자에 형상화한 인공습지에서 수행되었다. 적용되는 센서는 분산형으로 설치되는 LID를 고려하여 저비용으로 구축하고자 하였으며 비교적 저렴하면서 상용화되어있는 아두이노와 라즈베리 파이를 기반으로 하였다. 그리고 LID 시설의 현재 상태와 유지관리 및 이상기후 시 영향을 분석하기 위해 복합적인 센서 측정 개발에 목표를 두었다. 센서는 풍향·풍속, 강우량, 이산화탄소, 미세먼지, 온도·습도, 산성도, 위치 정보 등을 실시간으로 측정하도록 하였다. 또한 측정된 데이터의 수집, 전송 및 결과 확인을 위하여 데이터 수집 장치, 저장 서버 프로그램 및 PC와 모바일 활용 결과 확인 프로그램을 개발하였다. 각 센서를 통해 얻은 측정값들은 Wifi 모듈을 통해 관리 서버로 전달되고 실시간으로 데이터베이스 서버에 저장된다. 본 연구에서 수행한 4개월간의 측정 결과를 분석한 결과 LID 시설에 ICT 기술 적용의 안정성과 적용 가능성을 확인하였다. 실시간으로 측정된 값은 LID 시설의 기능 평가 및 유지관리 방안 도출을 위한 빅데이터 활용이 가능한 것으로 나타났다.