• Title/Summary/Keyword: Composite damage

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Inflammatory Effect of Light-Emitting Diodes Curing Light Irradiation on Raw264.7 Macrophage

  • Jeong, Moon-Jin;Kil, Ki-Sung;Lee, Myoung-Hwa;Lee, Seung-Yeon;Lee, Hye-Jin;Lim, Do-Seon;Jeong, Soon-Jeong
    • Journal of dental hygiene science
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    • v.19 no.2
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    • pp.133-140
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    • 2019
  • Background: The light-emitting diode (LED) curing light used is presumed to be safe. However, the scientific basis for this is unclear, and the safety of LED curing light is still controversial. The purpose of this study was to investigate the effect of LED curing light irradiation according to the conditions applied for the polymerization of composite resins in dental clinic on the cell viability and inflammatory response in Raw264.7 macrophages and to confirm the stability of LED curing light. Methods: Cell viability and cell morphology of Raw264.7 macrophages treated with 100 ng/ml of lipopolysaccharide (LPS) or/and LED curing light with a wavelength of 440~490 nm for 20 seconds were confirmed by methylthiazolydiphenyl-tetrazolium bromide assay and microscopic observation. The production of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) was confirmed by NO assay and $PGE_2$ enzyme-linked immunosorbent assay kit. Expression of interleukin $(IL)-1{\beta}$ and tumor necrosis factor $(TNF)-{\alpha}$ in total RNA and protein was confirmed by reverse transcription polymerase chain reaction and Western blot analysis. Results: The LED curing light did not affect the viability and morphology of normal Raw264.7 cells but affected the cell viability and induced cytotoxicity in the inflammation-induced Raw264.7 cells by LPS. The irradiation of the LED curing light did not progress to the inflammatory state in the inflammation-induced Raw264.7 macrophage. However, LED curing light irradiation in normal Raw264.7 cells induced an increase in NO and $PGE_2$ production and mRNA and protein expression of $(IL)-1{\beta}$ and $(TNF)-{\alpha}$, indicating that it is possible to induce the inflammatory state. Conclusion: The irradiation of LED curing light in RAW264.7 macrophage may induce an excessive inflammatory reaction and damage oral tissues. Therefore, it is necessary to limit the long-term irradiation which is inappropriate when applying LED curing light in a dental clinic.

Effects of red ginseng and Baeknyeoncho complex on the arthritis improvement (홍삼·백년초 복합물이 관절염 개선에 미치는 효과)

  • Lee, Seung-Hyeon;Lee, Chang-Hyun;Kim, Hong-Jun;Kim, Young-Sik;Shin, Min-Ji;Kim, Ji-Hyun;Ma, Sang-Yong;Lee, Se-Youn;Kwon, Jin;Oh, Chan-Ho
    • Herbal Formula Science
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    • v.29 no.2
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    • pp.81-91
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    • 2021
  • Objectives : This study was conducted to evaluate the efficacy of a complex mixture of natural substances of ginseng and baeknyeoncho on the arthritic rats. Methods : In vitro experiments were conducted to ensure the stability of the complex. After setting toxicity and concentration by MTT assay, the antioxidant effect was measured through DPPH and ABTS radical scavenging activity. To confirm the anti-inflammatory effects of the complex, levels of nitric oxide (NO) and pro-inflammatory cytokines (IL-1β, TNF-α) were measured in LPS-treated macrophage cell lines (RAW264.7). We injected monosodium iodoacetate (MIA) 50 μl (60 mg/ml) into knee joints of rats to induce osteoarthritis. The rats were divided into three groups (normal (n=5), control (n=5), and OR (n=5) group). The control group consumed 2 mg/kg of physiological saline once a day for 4 weeks, and the OR group was mixed at a concentration of 416.5 mg/kg of Baengnyeoncho (O) and 208.25 mg/kg of red ginseng (R) and ingested 1 mL each 5 days a week. Results : This complex increased the DPPH and ABTS radical scavenging rate. The complex decreased NO production and pro-inflammatory cytokine production of macrophages. In the OR group, the secretion of cytokine in serum was decreased. In histopathological examination, the joint tissue of the composite showed less damage to the synovial membrane, cartilage, and fibrous tissue than the control group. Conclusions : As a result of this study, natural complexes have antioxidant, anti-inflammatory and cartilage protection effects. Therefore, we expect the complex to be effective in treating osteoarthritis.

Fabrication of Electrospun Composite Membranes with Silk Powder (실크 입자가 도입된 전기방사 복합막 제조)

  • Seo, Young Jin;Kang, Hoseong;Im, Kwang Seop;Choi, Kang-min;Park, Chi Hoon;Nam, Sang Yong;Jang, Hae Nam
    • Membrane Journal
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    • v.32 no.2
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    • pp.133-139
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    • 2022
  • As the issue of reducing greenhouse gases is emerging due to global warming and extreme weather, research on materials capable of radiative cooling without energy consumption is being actively conducted. Among them, silk is known as a natural self-cooling material, but in the conventional mixing process using chemically powdered silk, there is a problem that the radiative cooling effect disappears by the collapses of the intrinsic crystal structure of silk fibroin, so it is difficult to manufacture it in the form of a film or coating agent for radiative cooling. In this study, various types of membranes were manufactured using silk powder that went through a physical pulverization process that does not damage the intrinsic structure of silk fibroin, and the study was conducted to examine its applicability as a coating agent. Electrospun membranes and flat sheet membranes were prepared by using silk fibroin powder for this purpose, and it was observed that the viscosity of the solution had a significant effect on the membrane fabrication and its properties.

Simulation of the effect of inclusions length and angle on the failure behavior of concrete structure under 3D compressive test: Experimental test and numerical simulation

  • Mohammad Saeed, Amini;Vahab, Sarfarazi;Kaveh, Asgari;Xiao, Wang;Mojtaba Moheb, Hoori
    • Steel and Composite Structures
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    • v.46 no.1
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    • pp.53-73
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    • 2023
  • Man-made structure materials like concrete usually contain inclusions. These inclusions affect the mechanical properties of concrete. In this investigation, the influence of inclusion length and inclination angle on three-dimensional failure mechanism of concrete under uniaxial compression were performed using experimental test and numerical simulation. Approach of acoustic emission were jointly used to analyze the damage and fracture process. Besides, by combining the stress-strain behavior, quantitative determination of the thresholds of crack stress were done. concrete specimens with dimensions of 120 mm × 150 mm × 100 mm were provided. One and two holes filled by gypsum are incorporated in concrete samples. To build the inclusion, firstly cylinder steel tube was pre-inserting into the concrete and removing them after the initial hardening of the specimen. Secondly, the gypsum was poured into the holes. Tensile strengths of concrete and gypsum were 2.45 MPa and 1.5 MPa, respectively. The angle bertween inclusions and axial loadind ary from 0 to 90 with increases of 30. The length of inclusion vary from 25 mm to 100 mm with increases of 25 mm. Diameter of the hole was 20 mm. Entirely 20 various models were examined under uniaxial test. Simultaneous with experimental tests, numerical simulation (Particle flow code in two dimension) were carried out on the numerical models containing the inclusions. The numerical model were calibrated firstly by experimental outputs and then failure behavior of models containing inclusions have been investigated. The angle bertween inclusions and axial loadind vary from 0 to 90 with increases of 15. The length of inclusion vary from 25 mm to 100 mm with increases of 25 mm. Entirely 32 various models were examined under uniaxial test. Loading rate was 0.05 mm/sec. The results indicated that when inclusion has occupied 100% of sample thickness, two tensile cracks originated from boundaries of sample and spread parallel to the loading direction until being integrated together. When inclusion has occupied 75% of sample thickness, four tensile cracks originated from boundaries of sample and spread parallel to the loading direction until being integrated together. When inclusions have occupied 50% and 25% of sample thickness, four tensile cracks originated from boundaries of sample and spread parallel to the loading direction until being integrated together. Also the inclusion was failed by one tensile crack. The compressive strength of samples decease with the decreases of the inclusions length, and inclusion angle had some effects on that. Failure of concrete is mostly due to the tensile crack. The behavior of crack, was affected by the inclusion length and inclusion number.

An Experimental Study for Characteristics Evaluation of Cement Mortar Using Infrared Thermography Technique (적외선 화상기법을 이용한 시멘트 모르타르 특성의 실험적 평가)

  • Kwon, Seung-Jun;Maria, Q. Feng
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.30 no.1A
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    • pp.53-59
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    • 2010
  • Recently, NDTs (Non-Destructive Techniques) using infrared camera are widely studied for detection of damage and void in RC (reinforced concrete) structures and they are also considered as an effective techniques for maintenance of infrastructures. The temperature on concrete surface depends on material and thermal properties such as specific heat, thermal conductivity, and thermal diffusion coefficient. Different porosity on cement mortar due to different mixture proportions can show different heat behavior in cooling stage. The porosity can affect physical and durability properties like strength and chloride diffusion coefficient as well. In this paper, active thermography which uses flash for heat induction is utilized and thermal characteristics on surface are evaluated. Samples of cement mortar with W/C (water to cement ratio) of 0.55 and 0.65 are prepared and physical properties like porosity, compressive strength, and chloride diffusion coefficient are evaluated. Then infrared thermography technique is carried out in a constant room condition (temperature $20{\sim}22^{\circ}C$ and relative humidity 55-60%). The mortar samples with higher porosity shows higher residual temperature at the cooling stage and also shows reduced critical time which shows constant temperature due to back wall effect. Furthermore, simple equation for critical time of back wall effect is suggested with porosity and experimental constants. These characteristics indicate the applicability of infrared thermography as an NDT for quality assessment of cement based composite like concrete. Physical properties and thermal behavior in cement mortar with different porosity are analyzed in discussed in this paper.

Stress analysis of high-temperature superconducting wire under electrical/magnetic/bending loads

  • Dongjin Seo;Yunjo Jung;Hong-Gun Kim;Hyung-Seop Shin;Young-Soon Kim
    • Progress in Superconductivity and Cryogenics
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    • v.25 no.4
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    • pp.19-23
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    • 2023
  • The Second-generation high-temperature superconducting (HTS) Rare-Earth Barium Copper Oxide (REBCO) wire is a composite laminate having a multi-layer structure (8 or more layers). HTS wires will undergo multiple loads including the bending-tension loads during winding, high current density, and high magnetic fields. In particular, the wires are subjected to bending stress and magnetic field stress because HTS wires are wound around a circular bobbin when making a high-field magnetic. Each of the different laminated wires inevitably exhibits damage and fracture behavior of wire due to stress deformation, mismatches in thermal, physical, electrical, and magnetic properties. Therefore, when manufacturing high-field magnets and other applications, it is necessary to calculate the stress-strain experienced by high-temperature superconducting wire to present stable operating conditions in the product's use environment. In this study, the finite element model (FEM) was used to simulate the strain-stress characteristics of the HTS wire under high current density and magnetic field, and bending loads. In addition, the result of obtaining the neutral axis of the wire and the simulation result was compared with the theoretical calculation value and reviewed. As a result of the simulation using COMSOL Multiphysics, when a current of 100 A was applied to the wire, the current value showed the difference of 10-9. The stress received by the wire was 501.9 MPa, which showed a theoretically calculated value of 500 MPa and difference of 0.38% between simulation and theoretical method. In addition, the displacement resulted is 30.0012 ㎛, which is very similar to the theoretically calculated value of 30 ㎛. Later, the amount of bending stress by the circular mandrel was received for each layer and the difference with the theoretically obtained the neutral axis result was compared and reviewed. This result will be used as basic data for manufacturing high-field magnets because it can be expanded and analyzed even in the case of wire with magnetic flux pinning.

Experimental Study on the Adhesion and Performance Evaluation of Joints for Modified Polyethylene Coated Steel Pipes (개질 폴리에틸렌 코팅 강관의 부착 및 체결부 성능 평가 연구)

  • Myung Kue Lee;Sanghwan Cho;Min Ook Kim
    • Composites Research
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    • v.37 no.3
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    • pp.238-245
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    • 2024
  • In this study, as part of the development of a monitoring system for the efficient maintenance of steel pipes, an experimental study was conducted to evaluate the performance of steel pipes treated with modified polyethylene coating. In the case of the conventional mechanical pre-coating method, there was a deterioration in polyethylene adhesion during expansion testing, which led to the application of a chemical pre-treatment process using a calcium-mixed phosphate zinc film to resolve this issue. SEM and EDX analyses showed that the densest structure was observed at a Zn/Ca ratio of 1.0, and improved heat resistance compared to the conventional method was confirmed. Additionally, to prevent coating detachment during expansion, an evaluation of adhesion and elongation was conducted on steel pipes with modified polyethylene coating, incorporating materials such as elastomers based on maleic anhydride grafting, metal oxides, blocking agents, and slip agents. Experimental results showed that the specimen (S4) containing all modified materials exhibited more than a 25% performance improvement compared to the specimen (S2) containing only metal oxides. Lastly, the development and performance evaluation of wedge-shaped socketing and pressing wheels, which are part of the pipe fixing accessories, were conducted to prevent surface coating damage on the completed pipes.

Mechanical Performance Study of Flexible Protection Tube for Submarine Cables (해저케이블용 유연보호튜브의 기계적 성능 연구)

  • Kyeong Soo Ahn;Yun Jae Kim;Jin-wook Choe;Jinseok Lim;Sung Woong Choi
    • Composites Research
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    • v.37 no.2
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    • pp.101-107
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    • 2024
  • Demand for submarine cable is increasing due to advances in submarine power transmission technology and submarine cable manufacturing technology. Submarine cable use various types of protective equipment to prevent problems such as high maintenance costs in the event of cable damage and power outages during maintenance periods. Among them, flexible protection tube is a representative protective equipment to protect cables and respond to external forces such as waves and current. The flexible protection tube is made of polyurethane 85A hyperelastic material, so the calculation of mechanical behavior is carried out using mechanical properties based on experimental results. In this study, a study was conducted to determine the bending performance and tensile performance of flexible protection tube through analytical methods. The physical properties obtained through the multiaxial tensile test of polyurethane 85A were used for the analysis. Bending and tensile performance were determined for the maximum bending moment standard of 15 kN·m and the tensile load standard of 50 kN. As a result, it was confirmed that when the maximum bending moment of 15 kN·m of the flexible protection tube occurred, the bending performance of the MBR was secured at 13 m and when a tensile load of 50 kN, it was applied the maximum vertical displacement was 968 mm, confirming that the tensile performance was secured.

TEMPERATURE CHANGE IN THE PULP ACCORDING TO POLISHING CONDITION OF VARIOUS RESTORATIVE MATERIALS (여러가지 수복물의 polishing조건에 따른 치수 온도변화)

  • Baik, Byeong-Ju;Park, Jong-Ha;Yang, Jeong-Suk;Lee, Seung-Young;Kim, Jae-Gon
    • Journal of the korean academy of Pediatric Dentistry
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    • v.26 no.2
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    • pp.365-376
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    • 1999
  • The importance of finishing and polishing the restoration has been described by several authors. The final step provides for improved metallurgical properties, better marginal adaptation, reduced plaque accumulation. Unfortunately, finishing of the restorations can produce damage from temperature rises at the pulpal wall. The aim of this study was to determine the changes in temperature can be occurred during the use of finishing and polishing instruments under a variety of conditions. ; with or without a water coolant, intermittent or continuous operation, high or low rotation speed, remaining dentin thickness and various restorative materials. Class V preparations were cut on extracted molars and restored with composite resin(Z 100), resin-modified glass ionomer cements(Dyract, Fuji II LC), and amalgam. Finishing was done with aluminum oxide coated disc($Sof-lex^{(R)}$ polishing disc, 3M, USA). The following results were obtained. 1. The rise of temperature during polishing of amalgam restorations was the highest among the all experimental groups except polishing with water coolant(P<0.05). However, there were no statistical differences in temperature rises between Z 100, Dyract and Fuji II LC(P>0.05). 2. The intrapulpal temperature was greatly influenced by the applied time, and intermittent polishing was showed significantly lower temperature rises than continuous polishing(P<0.01). 3. The intrapulpal temperature was increased according to the application of polishing regard less of using water coolant. However, polishing with water coolant showed significantly lower temperature in the pulp than not used water coolant(P<0.01).

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A study on the shear bond strengths of orthodontic brackets according to surface treatments and polymerizing techniques. (도재표면의 처리방법과 접착제의 중합방식에 따른 교정용 브라켓의 전단강도의 연구)

  • Kim, Young-Joo;Cha, Kyung-Suk;Lee, Jin-Woo
    • The korean journal of orthodontics
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    • v.29 no.4 s.75
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    • pp.445-456
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    • 1999
  • As a result of increased education and communication, the field of orthodontics has recently been expanded to include a greater number of adult treatment procedures. With this increased demand for adult orthodontic treatment, a problem that frequently arises is the placement of appliances on teeth restored with porcelain. But conventional acid-etching is ineffective in the preparation of porcelain surface for mechanical retention of orthodontic attachments. Also, it is possible to damage on porcelain. The purpose of this study was to evaluate the effect of composite bonding materials and the porcelain surface treatment methods on shear bond strength, and to observe the porcelain fracture rates. To accomplish this purpose, this study was carried out with feldsphatic porcelain, Ceram II. Porcelain surface treatment methods were divided into intact glazed porcelain which had not treatment and surface roughening. Surface roughening by etching with Hydroluoric acid(HF), sandblasting with Microetcher II and compound treatment with etching and sandblasting. Bonding materials were Ortho-two and Transbond. All porcelain specimens were applicated with porcelain primer. 1. In comparision according to porcelain surface treatment, surface roughening groups by HF etching and sandblasting had higher shear bond than intact group. No significant difference was found in Transbond group. 2. Ortho-two group had the higher shear bond strength than that of Transbond group in B:.u etching and sandblasting. 3. E(Transbond. Intact)group had the lowest shear bond strength in all experimental group. The bond strength was higher than clinically successful bond strength. 4. Non-treated group had very higher porcelain rates than treated group. 5. This study indicates that porcelain surface-roughening may not be necessary to attachment of orthodontic brackets to porcelain surfaces.

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